Abstract:
:Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testing of presumed pluripotent stem cells. The gold standard for pluripotent stem cells is to test if the cells are capable of contributing to germline chimeras. Differential staining can be used to evaluate the possibility of chimeric contribution; if the cells are located in the area of the ICM they are likely to contribute to the fetus and if they are located in the area of the TE they are likely to contribute to the fetal membranes. In this article, we optimize on methods for embryo staining and mounting so that the exact location of injected stem cells within preimplantation porcine embryos can be evaluated.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Secher JO,Freude KK,Li R,Callesen Hdoi
10.1089/scd.2014.0503subject
Has Abstractpub_date
2015-05-01 00:00:00pages
1141-5issue
9eissn
1547-3287issn
1557-8534journal_volume
24pub_type
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0252
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0351
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
pub_type: 杂志文章
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更新日期:2006-08-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0220
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章
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journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2009.0402
更新日期:2010-01-01 00:00:00
abstract::Embryonic stem cells (ESCs) have the capacity to form all the tissues in the body and hence, directed differentiation of ESCs along specific lineages represents a means to generate therapeutically useful cell types. It has been postulated that, during in vitro differentiation, ES cells sequentially pass through simila...
journal_title:Stem cells and development
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2012.0373
更新日期:2013-01-15 00:00:00
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journal_title:Stem cells and development
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更新日期:2016-05-01 00:00:00
abstract::Body axes and germ layers evolve at gastrulation, and in mammals are driven by many genes; however, what orchestrates the genetic pathways during gastrulation remains elusive. Previously, we presented evidence that microRNA-17 (miRNA-17) family members, miR-17-5p, miR-20a, miR-93, and miR-106a were differentially expr...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0051
更新日期:2015-02-01 00:00:00