Abstract:
:The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor-based cell therapy. However, the field of CPC biology suffers from the lack of human CPC-specific markers. Therefore, we raised a panel of monoclonal antibodies (mAb) against CPCs. Of this panel of antibodies, we show that mAb C1096 recognizes a progenitor-like population in the fetal and adult human heart and partially colocalize with reported CPC populations in vitro. Furthermore, mAb C1096 can be used to isolate a multipotent progenitor population from human heart tissue. Interestingly, the two lead candidates, mAb C1096 and mAb C19, recognize glycosylated residues on PECAM1 (platelet and endothelial cell adhesion molecule 1) and GRP78, respectively, and de-N-glycosylation significantly abolishes their binding. Thereby, this report describes new clinically applicable antibodies against human CPCs, and for the first time demonstrates the importance of glycosylated residues as CPCs specific markers.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Moerkamp AT,Leung HW,Bax NAM,Holst S,Lodder K,Berends T,Dingenouts CKE,Choo A,Smits AM,Goumans MJdoi
10.1089/scd.2017.0048subject
Has Abstractpub_date
2017-11-01 00:00:00pages
1552-1565issue
21eissn
1547-3287issn
1557-8534journal_volume
26pub_type
杂志文章abstract::In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0142
更新日期:2010-02-01 00:00:00
abstract::Cell replacement therapy holds great promise for treating a wide range of human disorders. However, ensuring the predictable differentiation of transplanted stem cells, eliminating their risk of tumor formation, and generating fully functional cells after transplantation remain major challenges in regenerative medicin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0555
更新日期:2011-11-01 00:00:00
abstract::Liver transplantation is the only radical treatment for decompensated cirrhosis, but its use is limited owing to a shortage of donors; hence, there is an urgent need for new treatments. Previously, we developed a liver-regeneration therapy using autologous bone marrow-derived mesenchymal stem cells (BMSCs), which is u...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0123
更新日期:2020-11-15 00:00:00
abstract::Chronic idiopathic neutropenia (CIN) is a granulopoiesis disorder associated with an inhibitory bone marrow (BM) microenvironment consisting of activated T-lymphocytes and pro-inflammatory mediators. In this study, we investigated the possible involvement of BM mesenchymal stem cells (MSCs) in the pathophysiology of C...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0425
更新日期:2011-08-01 00:00:00
abstract::Members of helix-loop-helix (HLH) protein family of Id (inhibitor of differentiation) dimerize with bHLH transcription factors and function as negative regulators of differentiation during development. Most of inhibitory roles of Id proteins have been demonstrated in non-neural tissues, and their roles in the developi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0093
更新日期:2010-06-01 00:00:00
abstract::The CD34 surface antigen has been recognized as a marker of hematopoietic stem cells (HSCs) and is widely used for HSC selection as well as for quality control in HSC transplantation. CD34 has been implicated in cytoadhesion signaling, and its expression has been suggested to reflect the activation state of hematopoie...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.0056
更新日期:2007-04-01 00:00:00
abstract::Lymphedema is a medically irreversible condition for which currently conservative and surgical therapies are either ineffective or impractical. The potential use of progenitor and stem cell-based therapies has offered a paradigm that may provide alternative treatment options for lymphatic disorders. Moreover, basic re...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0390
更新日期:2015-02-01 00:00:00
abstract::Human embryonic stem (hES) cells, like other stem cells, have the capacity to self-renew without differentiation. Although hES cells can be differentiated to many different tissue types in vitro, clinical uses have not yet been realized from the study of hES cells. Anticipation that these cells would be immediately us...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/154732804323099172
更新日期:2004-06-01 00:00:00
abstract::Previously, we have shown that increasing the intracellular cGMP concentration enhances the sonic hedgehog (Shh) response in neural plate cells. The use of two mouse embryonic stem (ES) cell lines allowed a highly sensitive and reproducible quantification of the Shh response in neuralized embryoid bodies. Here we demo...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.647
更新日期:2006-10-01 00:00:00
abstract::P311 is a newly discovered functional gene, and it has been proved to play a key role in blood pressure homeostasis, glioblastoma invasion, renal fibrosis, hypertrophic scar formation, and others. In this study, for the first time, we found that P311 could enhance reepithelialization during wound healing via promoting...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0249
更新日期:2017-03-15 00:00:00
abstract::There is surprising confusion surrounding the concept of biological totipotency, both within the scientific community and in society at large. Increasingly, ethical objections to scientific research have both practical and political implications. Ethical controversy surrounding an area of research can have a chilling ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0364
更新日期:2014-04-15 00:00:00
abstract::In the seminiferous epithelium of the testis, Sertoli cells are key niche cells directing proliferation and differentiation of spermatogonial stem cells (SSCs) into spermatozoa. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), which is essential for SSC self-renewal and progenitor expansion. W...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0318
更新日期:2017-04-15 00:00:00
abstract::White matter damage persists in hypoxic-ischemic newborns even when treated with hypothermia. We have previously shown that intraventricular delivery of human glial progenitors (GPs) at the neonatal stage is capable of replacing abnormal host glia and rescuing the lifespan of dysmyelinated mice. However, such transpla...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0172
更新日期:2019-04-15 00:00:00
abstract::Basic helix-loop-helix (bHLH) transcription factors are pivotal regulators of cellular differentiation and development. The bHLH factor, Twist-1 has previously been found to control bone marrow stromal/stem cells (BMSC) self-renewal, life span, and differentiation, however not much is known about its mechanism of acti...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0471
更新日期:2015-06-01 00:00:00
abstract::Embryonic stem cells (ESCs) have the capacity to form all the tissues in the body and hence, directed differentiation of ESCs along specific lineages represents a means to generate therapeutically useful cell types. It has been postulated that, during in vitro differentiation, ES cells sequentially pass through simila...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.656
更新日期:2005-12-01 00:00:00
abstract::The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0234
更新日期:2013-04-15 00:00:00
abstract::Bronchopulmonary dysplasia (BPD), the main complication of extreme prematurity, has lifelong consequences for lung health. Mesenchymal stromal cells (MSCs) prevent lung injury in experimental BPD in newborn rodents when given in the immediate neonatal period. Whether MSC therapy can restore normal lung growth after es...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0116
更新日期:2020-03-15 00:00:00
abstract::Human mesenchymal stromal cells (hMSCs) show great potential for clinical and experimental use due to their capacity to self-renew and differentiate into multiple mesenchymal lineages. However, disadvantages of primary cultures of hMSCs are the limited in vitro lifespan, and the variable properties of cells from diffe...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0599
更新日期:2014-10-01 00:00:00
abstract::Retinoic acid (RA) induces mouse F9 cells to form primitive endoderm (PrE) and increased levels of reactive oxygen species (ROS) accompany differentiation. ROS are obligatory for differentiation and while H2O2 alone induces PrE, antioxidants attenuate the response to RA. Evidence shows that ROS can modulate the Wnt/β-...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0010
更新日期:2014-05-15 00:00:00
abstract::Human stem cells from various sources have potential therapeutic applications. The clinical implementation of these therapies introduces the need for methods of noninvasive tracking of cells. The purpose of this study was to evaluate a high resolution magnetic resonance imaging (MRI) technique for in vivo detection an...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0028
更新日期:2008-12-01 00:00:00
abstract::Embryonic stem cell (ESC) research gave rise to the possibility that stem cell therapy could be used in the treatment of incurable diseases such as neurodegenerative disorders. However, problems related to the tumorigenicity of undifferentiated ESCs must be resolved before such cells can be used in the application of ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0523
更新日期:2011-01-01 00:00:00
abstract::A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0168
更新日期:2021-01-11 00:00:00
abstract::Studies on rodent bone marrow stromal cells (MSCs) have revealed a capacity, for at least a portion of cells, to express neuron-like traits after differentiation in culture. Little, however, is known about the ability of human MSCs in this regard. We show here that incubation with certain differentiation cocktails, pa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2004.13.625
更新日期:2004-12-01 00:00:00
abstract::Mesenchymal stems cells (MSCs) are a population of multipotent cells residing in several readily available adult tissue compartments, thus allowing for their ex vivo expansion. To date, therapeutic applications of MSCs have focused on their ability to hone to and support the repair of damaged tissues. More recent evid...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2011.0404
更新日期:2012-02-10 00:00:00
abstract::Human embryonic stem cells (hESCs) exhibiting skewed X chromosome inactivation (XCI) have been reported. The copy number variations (CNVs), loss of heterozygosity (LOH), or single-nucleotide variant (SNV) events in those epigenetically distinct cells remain unknown, and whether such genetic abnormalities will influenc...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0463
更新日期:2015-08-01 00:00:00
abstract::We have previously isolated mesenchymal stromal cells (MSCs) from the tracheal aspirates of premature neonates with respiratory distress. Although isolation of MSCs correlates with the development of bronchopulmonary dysplasia, the physiologic role of these cells remains unclear. To address this, we further characteri...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0494
更新日期:2011-11-01 00:00:00
abstract::Death ligands and their tumor necrosis factor receptor (TNFR) family receptors are the best-characterized and most efficient inducers of apoptotic signaling in somatic cells. In this study, we analyzed whether these prototypic activators of apoptosis are also expressed and able to be activated in human pluripotent ste...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0057
更新日期:2013-11-15 00:00:00
abstract::Administration of human bone marrow-derived mesenchymal stem cells (BM-MSCs) significantly alleviates allergic airway inflammation. There are no studies that refer to the role of microRNAs (miRNAs) after the BM-MSCs treatment in airway allergic inflammation. We induced a mouse model of asthma and performed the transpl...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0339
更新日期:2016-06-15 00:00:00
abstract::The secretome of human amniotic fluid stem cells (AFSCs) has great potential as a therapeutic agent in regenerative medicine. However, it must be produced in a clinically compliant manner before it can be used in humans. In this study, we developed a means of producing a biologically active secretome from AFSCs that i...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0089
更新日期:2017-09-15 00:00:00
abstract::Retinoic acid (RA) is present at sites of neurogenesis in both the embryonic and adult brain. While it is widely accepted that RA signaling is involved in the regulation of neural stem cell differentiation, little is known about vitamin A utilization and biosynthesis of active retinoids in the neurogenic niches, or ab...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0422
更新日期:2013-10-15 00:00:00