Abstract：
:Mesenchymal stem cells (MSCs) have attracted much attention as potential platforms for transgene delivery and cell-based therapy for human disease. MSCs have the capability to self-renew and retain multipotency after extensive expansion in vitro, making them attractive targets for ex vivo modification and autologous transplantation. Viral vectors, including lentiviral vectors, provide an efficient means for transgene delivery into human MSCs. In contrast, mouse MSCs have proven more difficult to transduce with lentiviral vectors than their human counterparts, and because many studies use mouse models of human disease, an improved method of transduction would facilitate studies using ex vivo-modified mouse MSCs. We have worked toward improving the production of human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors and optimizing transduction conditions for mouse MSCs using lentivirus vectors pseudotyped with the vesicular stomatitis virus G glycoprotein (VSV-G), the ecotropic murine leukemia virus envelope glycoprotein (MLV-E), and the glycoproteins derived from the Armstrong and WE strains of lymphocytic choriomeningitis virus (LCMV-Arm, LCMV-WE). Mouse MSCs were readily transduced following overnight incubation using a multiplicity of infection of at least 40. Alternatively, mouse MSCs in suspension were readily transduced after a 1-h exposure to lentiviral pseudotypes immediately following trypsin treatment or retrieval from storage in liquid nitrogen. LCMV-WE pseudotypes resulted in efficient transduction of mouse MSCs with less toxicity than VSV-G pseudotypes. In conclusion, our improved production and transduction conditions for lentiviral vectors resulted in efficient transduction of mouse MSCs, and these improvements should facilitate the application of such cells in the context of mouse models of human disease.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Ricks DM,Kutner R,Zhang XY,Welsh DA,Reiser Jdoi
10.1089/scd.2007.0194subject
Has Abstractpub_date
2008-06-01 00:00:00pages
441-50issue
3eissn
1547-3287issn
1557-8534journal_volume
17pub_type
杂志文章abstract：:We determined whether cartilage could be engineered from mesenchymal progenitor cells (MPCs) normally found in amniotic fluid. Mesenchymal amniocytes were isolated from ovine amniotic fluid samples (n = 5) and had their identity confirmed by immunocytochemistry. Cells were expanded and then cultured as micromass pelle...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2006.15.245
更新日期：2006-04-01 00:00:00
abstract：:Aneuploidy is commonly seen in human preimplantation embryos, most particularly at the cleavage stage because of genome activation by third cell division. Aneuploid embryos have been used for the derivation of normal embryonic stem cell (ESC) lines and developmental modeling. This review addresses aneuploidies in huma...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2013.0053
更新日期：2013-09-01 00:00:00
abstract：:Cell replacement therapy holds great promise for treating a wide range of human disorders. However, ensuring the predictable differentiation of transplanted stem cells, eliminating their risk of tumor formation, and generating fully functional cells after transplantation remain major challenges in regenerative medicin...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0555
更新日期：2011-11-01 00:00:00
abstract：:The enhanced proliferation of mesenchymal stem cells (MSCs) can be helpful for the clinical translation of cell therapy. Low-level laser irradiation (LLLI) has been demonstrated as regulating MSC proliferation. MicroRNAs (miRNAs) are involved in various pathophysiologic processes in stem cells, but the role of miRNAs ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0695
更新日期：2012-09-01 00:00:00
abstract：:Subarachnoid hemorrhage (SAH) is a type of hemorrhagic stroke with significant morbidity and mortality, and it often leads to poor clinical outcome. Although great efforts have been made toward animal and clinical studies, optimal therapy of SAH remains a challenge for scientists and clinicians. Increasing evidence su...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2019.0219
更新日期：2020-02-15 00:00:00
abstract：:Regenerative medicine, a paragon of future healthcare, holds unprecedented potential in extending the reach of treatment modalities for individuals across diseases and lifespan. Emerging regenerative technologies, focused on structural repair and functional restoration, signal a radical transformation in medical and s...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0448
更新日期：2013-12-01 00:00:00
abstract：:The regenerative capacity of the endometrium has been attributed to resident stem/progenitor cells. A number of stem/progenitor markers have been reported for human endometrial stem/progenitor cells; however, the lack of convenient markers in the mouse has made experimental investigation into endometrial regeneration ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2018.0133
更新日期：2019-01-01 00:00:00
abstract：:Cell mobilization, a process that influences circulation, margination, and finally, homing play key roles in the regeneration processes mediated by stem cells. Recent studies as well as prior studies from our group indicate an important role of the spleen in hematopoietic reconstitution, but to date the role of the sp...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0068
更新日期：2012-03-20 00:00:00
abstract：:The hedgehog (Hh) signaling pathway plays fundamental roles during embryonic development and tumorigenesis. Previously, we have shown that ablation of the tumor suppressor and negative regulator, Suppressor of fused (Sufu), within this pathway causes embryonic lethality around E9.5 in the mouse. In this study, we exam...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0050
更新日期：2015-11-01 00:00:00
abstract：:There are a limited number of therapies available to prevent heart failure following myocardial infarction. One novel therapy that is currently being pursued is the implantation of cardiac progenitor cells (CPCs); however, their responses to oxidative stress during differentiation have yet to be elucidated. The object...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0673
更新日期：2013-09-01 00:00:00
abstract：:The regeneration of lost periodontal ligament (PDL) and alveolar bone is the purpose of periodontal tissue engineering. The goal of the present study was to assess the suitability of 3 odontogenic progenitor populations from dental pulp, PDL, and dental follicle for periodontal regeneration when exposed to natural and...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2010.0431
更新日期：2011-10-01 00:00:00
abstract：:The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0234
更新日期：2013-04-15 00:00:00
abstract：:In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0588
更新日期：2014-07-15 00:00:00
abstract：:Members of helix-loop-helix (HLH) protein family of Id (inhibitor of differentiation) dimerize with bHLH transcription factors and function as negative regulators of differentiation during development. Most of inhibitory roles of Id proteins have been demonstrated in non-neural tissues, and their roles in the developi...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0093
更新日期：2010-06-01 00:00:00
abstract：:Periodontitis is an infectious disease characterized by chronic inflammation and progressive destruction of periodontal tissues. Chronic inflammatory environment may affect immunomodulatory function of periodontal ligament stem cells (PDLSCs) and promote shift toward proinflammatory phenotype contributing to propagati...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2019.0021
更新日期：2019-08-01 00:00:00
abstract：:Embryonic stem (ES) cells give rise to mesodermal progenitors that differentiate to hematopoietic and cardiovascular cells. The wnt signaling pathway plays multiple roles in cardiovascular development through a network of intracellular effectors. To monitor global changes in wnt signaling during ES cell differentiatio...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0326
更新日期：2012-01-01 00:00:00
abstract：:The corneal endothelium is believed to be developmentally originated from the periocular mesenchyme via the neural crest. Human corneal endothelial progenitor cells (HCEPs) have been investigated because of their potential availability for the tissue regenerative medicine. However, the existence and the properties of ...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2013.0387
更新日期：2014-09-15 00:00:00
abstract：:The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2011.0600
更新日期：2012-08-10 00:00:00
abstract：:Fertility clinics worldwide routinely produce a large volume of 'waste' follicular aspirate, which is potentially an abundant source of immature ovarian follicles. Current attempts to cultivate these further in vitro to yield viable mature oocytes for fertility treatment have not yet achieved much success. Instead, re...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2005.14.11
更新日期：2005-02-01 00:00:00
abstract：:Diseases of the digestive tract are complex and encompass a broad spectrum of different pathogeneses (inflammatory, ischemic, neoplastic, and functional deficit). The digestive tract is not a sterile environment, and its organs are composed of tissues with different embryologic origin and different morphologic and fun...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2010.0277
更新日期：2011-07-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) from bone marrow (BM) are widely used for bone and less for cartilage tissue regeneration due to their self-renewal and differentiating properties into osteogenic or chondrogenic lineages. This review considers the last decade of clinical trials involving a two-step procedure, by expandin...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2012.0373
更新日期：2013-01-15 00:00:00
abstract：:Central nervous system (CNS) inflammation is primarily driven by microglial cells which secrete proinflammatory cytokines and undergo proliferation upon activation, as it occurs in neurodegenerative diseases. Uncontrolled or prolonged CNS inflammation is potentially harmful and can result in cellular damage. Recently,...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0268
更新日期：2016-07-15 00:00:00
abstract：:Human embryonic stem cells (hESCs) exhibiting skewed X chromosome inactivation (XCI) have been reported. The copy number variations (CNVs), loss of heterozygosity (LOH), or single-nucleotide variant (SNV) events in those epigenetically distinct cells remain unknown, and whether such genetic abnormalities will influenc...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2014.0463
更新日期：2015-08-01 00:00:00
abstract：:Fluorescence in situ hybridization (FISH) was used to analyze peripheral blood stem cells (PBSC) and stem cell assays (SCA) derived from them in 2 patients with acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality prior to undergoing autologous stem cell transplantation. In both cases, the de...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/154732804773099227
更新日期：2004-02-01 00:00:00
abstract：:Progenitor expansion during development is a highly regulated process dictating the final organ size, while expansion of specific progenitor populators can adjust the final cellular composition of the organ. Understanding factors involved in these pathways is required to develop cell-based therapies such as β-cell tra...
journal_title：Stem cells and development
pub_type： 杂志文章,评审
doi：10.1089/scd.2013.0106
更新日期：2013-11-15 00:00:00
abstract：:Embryonic stem cells (ESCs) posses the ability to self-renew and differentiate into a multitude of lineages, including the osteogenic lineage in vitro. Currently, most approaches have focused on embryonic body (EB)-mediated osteogenic differentiation, which relies on formation of all three germ layers resulting in lim...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2007.0228
更新日期：2008-10-01 00:00:00
abstract：:Administration of human bone marrow-derived mesenchymal stem cells (BM-MSCs) significantly alleviates allergic airway inflammation. There are no studies that refer to the role of microRNAs (miRNAs) after the BM-MSCs treatment in airway allergic inflammation. We induced a mouse model of asthma and performed the transpl...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2015.0339
更新日期：2016-06-15 00:00:00
abstract：:Myocardial infarction is a major public health problem that causes significant mortality despite recent advances in its prevention and treatment. Therefore, approaches based on adult stem cells represent a promising alternative to conventional therapies for this life-threatening condition. Mesenchymal stem cells (MSCs...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2008.0292
更新日期：2009-07-01 00:00:00
abstract：:Bone marrow human mesenchymal stem cells (hMSCs) are known to survive in serum-free media, when most normal somatic cells do not survive. We found that the endogenously-activated bone morphogenetic protein (BMP) pathway is involved in this cellular behavior. Under this culture condition, phosphorylated Smad1 (PSmad1),...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2009.0020
更新日期：2009-11-01 00:00:00
abstract：:There is an increasing need for an animal model that can be used to translate basic research into clinical therapy. We documented the differentiation and functional competence of embryonic stem cell (ESC)-derived endothelial cells in baboons. Baboon angioblasts were sequentially differentiated from embryoid body cultu...
journal_title：Stem cells and development
pub_type： 杂志文章
doi：10.1089/scd.2012.0313
更新日期：2013-02-15 00:00:00
