Abstract:
:The development of methods to induce differentiation of human mesenchymal stem cells (hMSCs) has opened the possibility of using these cells in regenerative or reparative therapies. However, the low frequency of hMSCs in tissue means it is often necessary to expand these cells extensively in vitro. In this study, we evaluated the effects of long-term serial passage on the characteristics of bone marrow-derived hMSC populations. In addition, we examined the effect on subsequent hMSC neural differentiation ability, which has not been reported earlier. The hMSC population examined was found to maintain a stable phenotype during the first 6-8 passages of culture as assessed by proliferative ability, morphological appearance, and surface antigen, gene and protein expression, and also expressed pluripotency and neural lineage markers constitutively in the undifferentiated state. Long-term subcultivation neither resulted in spontaneous neural differentiation nor compromised the ability of hMSCs to develop toward an early neuronal fate. In addition, the transformation elicited in hMSC cultures in response to cytokine-based neuronal differentiation was examined by live cell microscopy. We demonstrated, for the first time, that the observed changes result from active and dynamic processes involving outgrowth and motility of cellular extensions, processes entirely distinct from the rapid epiphenomena of cytotoxicity and cytoskeleton disruption generated by chemical induction methods. Cytokine-induced differentiation of hMSCs was also associated with upregulation of early neural gene and protein expression. These findings support the neuronal differentiation capability of hMSCs, although further investigation is required to confirm the ability to attain a mature neuronal phenotype.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Khoo ML,Shen B,Tao H,Ma DDdoi
10.1089/scd.2007.0185subject
Has Abstractpub_date
2008-10-01 00:00:00pages
883-96issue
5eissn
1547-3287issn
1557-8534journal_volume
17pub_type
杂志文章abstract::The mental retardation-associated protein, srGAP3 is highly expressed in neurogenic sites. It is thought to regulate the key aspects of neuronal development and functions. Little is known about the interaction between srGAP3 and immature neural stem cells/neural progenitor cells (NSCs/NPCs). In the current study, the ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0455
更新日期:2013-06-01 00:00:00
abstract::Chronic idiopathic neutropenia (CIN) is a granulopoiesis disorder associated with an inhibitory bone marrow (BM) microenvironment consisting of activated T-lymphocytes and pro-inflammatory mediators. In this study, we investigated the possible involvement of BM mesenchymal stem cells (MSCs) in the pathophysiology of C...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0425
更新日期:2011-08-01 00:00:00
abstract::Administration of human bone marrow-derived mesenchymal stem cells (BM-MSCs) significantly alleviates allergic airway inflammation. There are no studies that refer to the role of microRNAs (miRNAs) after the BM-MSCs treatment in airway allergic inflammation. We induced a mouse model of asthma and performed the transpl...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0339
更新日期:2016-06-15 00:00:00
abstract::Phenotypic guidance of embryonic stem (ES) cell fate is paramount if these cells are to be used for tissue repair and regeneration. Our objective was to compare two different cell culture feeders and their effect on proliferation, apoptosis, and differentiation of human (h) ES cells. HSF-6 hES cells were grown in Knoc...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.0109
更新日期:2007-06-01 00:00:00
abstract::The field of regenerative medicine has increasingly recognized the importance to be inspired by developmental processes to identify signaling pathways crucial for 3D organogenesis and tissue regeneration. Here, we aimed at recapitulating the first events occurring during limb development (ie, cell condensation and exp...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0216
更新日期:2013-11-01 00:00:00
abstract::Inducing a stable and predictable program of neural cell fate in pluripotent cells in vitro is an important goal for utilizing these cells for modeling human disease mechanisms. However, the extent to which in vitro neural specification recapitulates in vivo neural specification remains to be fully established. We pre...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0593
更新日期:2012-10-10 00:00:00
abstract::Bronchopulmonary dysplasia (BPD), the main complication of extreme prematurity, has lifelong consequences for lung health. Mesenchymal stromal cells (MSCs) prevent lung injury in experimental BPD in newborn rodents when given in the immediate neonatal period. Whether MSC therapy can restore normal lung growth after es...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0116
更新日期:2020-03-15 00:00:00
abstract::The placenta is a transient organ that develops upon the initiation of pregnancy and is essential for embryonic development and fetal survival. The rodent placenta consists of distinct lineages and includes cell types that are analogous to those that make up the human placenta. Trophoblast cells within the labyrinth l...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0092
更新日期:2014-12-01 00:00:00
abstract::The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0234
更新日期:2013-04-15 00:00:00
abstract::Transplantation of retinal cells has recently provided a promising therapeutic approach for retinal degeneration. Here, we differentiated initially retinal progenitors (RPs) from adherent feeder-free human embryonic stem cells (hESCs) with the use of defined media supplemented with a specific combination of growth fac...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0073
更新日期:2012-01-01 00:00:00
abstract::The success rate of human embryonic stem cell (hESC) derivation depends on both culture conditions and embryo quality and is routinely determined by morphological criteria. However, high incidence of chromosomal abnormality even in high-grade cleavage embryos from in vitro fertilization (IVF) patients suggests that th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0136
更新日期:2010-01-01 00:00:00
abstract::Herein we review recent data that support host tolerance of allogeneic adult mesenchymal stem cells (MSC). Evidence is emerging that donor MSC deploy a very powerful array of mechanisms that allow escape from host allogeneic responses. These mechanisms include limited expression of alloantigen by the stem cell and cel...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2005.14.252
更新日期:2005-06-01 00:00:00
abstract::Mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. The elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. In this study, we used an ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0685
更新日期:2013-07-15 00:00:00
abstract::Extracellular stresses influence transcription factor (TF) expression and therefore lineage identity in the peri-implantation mouse embryo and its stem cells. This potentially affects pregnancy outcome. To understand the effects of stress signaling during this critical period of pregnancy, we exposed cultured murine e...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0157
更新日期:2014-12-15 00:00:00
abstract::Special features of mesenchymal stem cells (MSCs) have made them a popular tool in cell therapy and tissue engineering. Although mouse animal models and murine MSCs are common tools in this field, our understanding of the effect of in vitro expansion on the behavior of these cells is poor and controversial. In additio...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0266
更新日期:2011-08-01 00:00:00
abstract::Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect of GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth in vitro and of animal models on hematopoietic rege...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0230
更新日期:2015-03-15 00:00:00
abstract::A murine stromal cell line (OP9-DL1) expressing a notch ligand, Delta-like-1, has been shown to be able to drive the differentiation of both murine and human hematopoietic progenitors into T cells in vitro. Further studies showed that hematopoietic progenitors transduced by a retroviral vector to express a human CD8 T...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0021
更新日期:2009-03-01 00:00:00
abstract::Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0503
更新日期:2015-05-01 00:00:00
abstract::Aneuploidy is commonly seen in human preimplantation embryos, most particularly at the cleavage stage because of genome activation by third cell division. Aneuploid embryos have been used for the derivation of normal embryonic stem cell (ESC) lines and developmental modeling. This review addresses aneuploidies in huma...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0053
更新日期:2013-09-01 00:00:00
abstract::Self-renewal and differentiation of endogenous stem cells (SCs) are essential for adult tissue homoeostasis and intrinsic healing capacity. In this study, we hypothesize that penis contains a small population of endogenous SCs, which might help rejuvenation of damaged erectile function. In this study, 60 newborn male ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0380
更新日期:2015-03-15 00:00:00
abstract::Pluripotent stem cell-derived endothelial cells (ECs) have great potential to be used in vascular therapy or tissue engineering. It is also much desired to obtain arterial or venous ECs for specific applications. Factors that are critical for the proper arterial or venous differentiation from pluripotent stem cells st...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0240
更新日期:2018-04-01 00:00:00
abstract::Dental follicle stem cells (DFSCs), which are the stem cells present in the dental follicle tissue of the tooth germ and are derived from the neural crest, are direct precursor cells of periodontal tissues and can form the periodontal ligament, cementum, and alveolar bone proper in the late stage of tooth development....
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2019.0012
更新日期:2019-08-01 00:00:00
abstract::Regenerative therapy of the salivary gland (SG) is a promising therapeutic approach for irreversible hyposalivation in patients with head and neck cancer treated by radiotherapy. However, little is known about the molecular regulators of stem/progenitor cell activity and regenerative processes in the SG. Wnt/β-catenin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0499
更新日期:2010-11-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0029
更新日期:2018-01-15 00:00:00
abstract::Human embryonic stem (HUES) cells are derived from early individual embryos with unique genetic printing. However, how their epigenetic status might affect their potential to differentiate toward specific lineages remains a puzzling question. Using chromatin immunoprecipitation (ChIP)-polymerase chain reaction and ChI...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0345
更新日期:2015-02-01 00:00:00
abstract::Resveratrol is a natural polyphenol antioxidant that has been shown to facilitate osteogenic differentiation. A recent breakthrough has demonstrated that ectopic expression of four genes is sufficient to reprogram murine and human fibroblasts into induced pluripotent stem (iPS) cells. However, the roles of resveratrol...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0186
更新日期:2010-02-01 00:00:00
abstract::The clinical use of stem cells in cell-based therapeutics for degenerative diseases requires development of criteria for defining normal stem cells to ensure safe transplantation. Currently, identification of abnormal from normal stem cells is based on extensive ex vivo and in vivo testing. Raman microscopy is a label...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0600
更新日期:2012-08-10 00:00:00
abstract::With the recent advances in regenerative medicine, nanotechnology has created a niche for itself as a promising avenue in this field. Innumerable studies have been carried out by researchers using virus-based methodologies for the purpose of epigenetic reprogramming. Although this method is ostensibly safe, nonetheles...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0309
更新日期:2015-03-01 00:00:00
abstract::Misexpression of the double homeodomain protein DUX4 in muscle is believed to cause facioscapulohumeral muscular dystrophy (FSHD). Although strategies are being devised to inhibit DUX4 activity in FSHD, there is little known about the normal function of this protein. Expression of DUX4 has been reported in pluripotent...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0643
更新日期:2013-09-01 00:00:00
abstract::Human mesenchymal stem cells (MSCs) are a promising tool for therapeutic applications in cell-based therapy and regenerative medicine, and MSCs from the human palatine tonsils have recently been used as a new tissue source. However, the understanding of the proliferation and differentiation capacity of tonsil-derived ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0061
更新日期:2016-08-01 00:00:00