Abstract:
:Transcription factor FoxA1 plays a critical role during embryonic development and is activated during retinoic acid (RA)-induced neural differentiation of pluripotent P19 embryonal carcinoma cells at the early stage, which is marked by decreased expression of Nanog and increased expression of neural stem cell marker Nestin. To further understand how FoxA1 mediates neural differentiation, we have overexpressed FoxA1 through an adenovirus vector in P19 cells and identified that early neurogenesis-related sonic hedgehog (Shh) gene is activated directly by FoxA1. Knockdown of FoxA1 expression during P19 cell neural differentiation results in prevention of Shh and Nestin induction. FoxA1 binds to Shh promoter at -486 to -462 bp region and activates the promoter in cotransfection assays. Furthermore, overexpression of FoxA1 alone in P19 cells stimulates expression of Nestin and results in decreased protein levels of Nanog. During RA-induced P19 cell differentiation, elevated levels of FoxA1 increase the population of neurons, evidenced by stimulated expression of neuron-specific Neurofilament-1 and Tubulin betaIII. Together, our results suggest a critical involvement of FoxA1 in stimulating neural differentiation of pluripotent stem cells at early stages.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Tan Y,Xie Z,Ding M,Wang Z,Yu Q,Meng L,Zhu H,Huang X,Yu L,Meng X,Chen Ydoi
10.1089/scd.2009.0386subject
Has Abstractpub_date
2010-09-01 00:00:00pages
1365-74issue
9eissn
1547-3287issn
1557-8534journal_volume
19pub_type
杂志文章abstract::The mammalian inner ear is vulnerable to genetic disorders and aging, as well as to injuries caused by overstimulation, ototoxic drugs, and viral infections. Due to the poor regeneration of the sensory epithelium and the spiral ganglion neurons in the adult mammalian inner ear, cell replacement therapy strategies have...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2006.15.449
更新日期:2006-06-01 00:00:00
abstract::Embryonic stem cell (ESC) research gave rise to the possibility that stem cell therapy could be used in the treatment of incurable diseases such as neurodegenerative disorders. However, problems related to the tumorigenicity of undifferentiated ESCs must be resolved before such cells can be used in the application of ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0523
更新日期:2011-01-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) hold great promise in the field of regenerative medicine due to their ability to create a variable localized anti-inflammatory effect in injuries such as Crohn's disease and osteoarthritis or by incorporation in tissue engineered constructs. Currently, the MSC literature uses rodents f...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0145
更新日期:2020-06-01 00:00:00
abstract::Rare heterozygous deletions in the neurexin 1 (NRXN1) gene robustly increase an individual's risk of developing neurological and psychiatric disorders. However, the molecular bases by which different mutations result in different clinical presentations, with variable penetrance, are unknown. To better understand the m...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0017
更新日期:2020-09-01 00:00:00
abstract::Tendons and ligaments (T/L) are dense connective tissues of mesodermal origin. During embryonic development, the tendon-specific cells descend from a sub-set of mesenchymal progenitors condensed in the syndetome, a dorsolateral domain of the sclerotome. These cells are defined by the expression of the transcription fa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0150
更新日期:2012-04-10 00:00:00
abstract::The CD34 surface antigen has been recognized as a marker of hematopoietic stem cells (HSCs) and is widely used for HSC selection as well as for quality control in HSC transplantation. CD34 has been implicated in cytoadhesion signaling, and its expression has been suggested to reflect the activation state of hematopoie...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.0056
更新日期:2007-04-01 00:00:00
abstract::Cell replacement therapy holds great promise for treating a wide range of human disorders. However, ensuring the predictable differentiation of transplanted stem cells, eliminating their risk of tumor formation, and generating fully functional cells after transplantation remain major challenges in regenerative medicin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0555
更新日期:2011-11-01 00:00:00
abstract::Retinoic acid (RA) induces mouse F9 cells to form primitive endoderm (PrE) and increased levels of reactive oxygen species (ROS) accompany differentiation. ROS are obligatory for differentiation and while H2O2 alone induces PrE, antioxidants attenuate the response to RA. Evidence shows that ROS can modulate the Wnt/β-...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0010
更新日期:2014-05-15 00:00:00
abstract::After intra-articular injection, synovium-derived mesenchymal stem cells (SMSCs) can adhere to damaged cartilage (a process called homing) and then repair the cartilage defect. Nonetheless, the main obstacle of the current method is the insufficient homing ratio of SMSCs, which fails to meet the requirements for carti...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0137
更新日期:2020-04-01 00:00:00
abstract::Lymphedema is a medically irreversible condition for which currently conservative and surgical therapies are either ineffective or impractical. The potential use of progenitor and stem cell-based therapies has offered a paradigm that may provide alternative treatment options for lymphatic disorders. Moreover, basic re...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0390
更新日期:2015-02-01 00:00:00
abstract::Regenerating islet (Reg) proteins are involved in the proliferation and differentiation of diverse cell types. However, whether embryonic stem cells (ESCs) express Reg genes and their corresponding proteins remains unknown. In this study, we probed the expression of Reg family members by mouse ESCs (mESCs). Mouse Reg1...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0398
更新日期:2010-09-01 00:00:00
abstract::Conditioned media (CM) of transformed cells, such as the human lung-derived A549 cells, is a useful tool for directing differentiation of embryonic stem cells (ESCs). Previous work indicates that A549-CM induced pulmonary differentiation of mouse ESCs (mESCs). In this study, we compared the effects of A549-CM treatmen...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0042
更新日期:2014-08-15 00:00:00
abstract::Bronchopulmonary dysplasia (BPD), the main complication of extreme prematurity, has lifelong consequences for lung health. Mesenchymal stromal cells (MSCs) prevent lung injury in experimental BPD in newborn rodents when given in the immediate neonatal period. Whether MSC therapy can restore normal lung growth after es...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0116
更新日期:2020-03-15 00:00:00
abstract::Cell therapy is a promising novel option for treatment of cardiovascular disease. Because the role of bone marrow-derived circulating progenitor cells (BM-CPCs) after cell therapy is less clear, we analyzed in this randomized, controlled study the influence of intracoronary autologous freshly isolated bone marrow cell...
journal_title:Stem cells and development
pub_type: 杂志文章,随机对照试验
doi:10.1089/scd.2010.0373
更新日期:2011-09-01 00:00:00
abstract::Progenitor expansion during development is a highly regulated process dictating the final organ size, while expansion of specific progenitor populators can adjust the final cellular composition of the organ. Understanding factors involved in these pathways is required to develop cell-based therapies such as β-cell tra...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2013.0106
更新日期:2013-11-15 00:00:00
abstract::Traumatic spinal cord injury (SCI) leads to a cascade of secondary chemical insults, including oxidative stress and glutamate excitotoxicity, which damage host neurons and glia. Transplantation of exogenous neural stem/progenitor cells (NSPCs) has shown promise in enhancing regeneration after SCI, although survival of...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0389
更新日期:2016-08-15 00:00:00
abstract::The field of regenerative medicine has increasingly recognized the importance to be inspired by developmental processes to identify signaling pathways crucial for 3D organogenesis and tissue regeneration. Here, we aimed at recapitulating the first events occurring during limb development (ie, cell condensation and exp...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0216
更新日期:2013-11-01 00:00:00
abstract::Tissue engineering and cell implantation therapies are gaining popularity because of their potential to repair and regenerate tissues and organs. To investigate the role of inflammatory cytokines in new tissue development in engineered tissues, we have characterized the nature and timing of cell populations forming ne...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0451
更新日期:2013-05-15 00:00:00
abstract::Human embryonic stem cells (hESCs) have the potential to differentiate into many adult cell types, and they are being explored as a resource for cell replacement therapies for multiple diseases. In order to optimize in vitro differentiation protocols, it will be necessary to elucidate regulatory mechanisms that contri...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0224
更新日期:2010-06-01 00:00:00
abstract::In recent years it has been hypothesized that cancer stem cells (CSCs) are the actual driving force of tumor formation, highlighting the need to specifically target CSCs to successfully eradicate cancer growth and recurrence. Particularly, the deregulation of physiological signaling pathways controlling stem cell prol...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2015.0081
更新日期:2015-10-15 00:00:00
abstract::Stem cell factor (SCF) stimulation of the receptor tyrosine kinase c-kit has effects on the proliferation, differentiation, and apoptotic regulation of hematopoietic progenitor cell populations. Rat bone marrow myelomonocytic stem cells (MSC) isolated in vitro by wheat germ agglutinin culture exclusively undergo self-...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.556
更新日期:2005-10-01 00:00:00
abstract::We describe a rapid and efficient 5-step program of defined factors for the genesis of brain myelin-forming oligodendrocytes (OLs) from embryonic stem cells (ESCs). The OLs emerge on the same time frame in vitro as seen in vivo. Factors promoting neural induction (retinoids, noggin) are required, while exogenous Sonic...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0520
更新日期:2010-09-01 00:00:00
abstract::In future, adipose-derived stem cells (ASC) might be used to treat neurological disorders. In this study, the neurotrophic and angiogenic properties of human ASC were evaluated, and their effects in a peripheral nerve injury model were determined. In vitro growth factor stimulation of the cells resulted in increased s...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0396
更新日期:2014-04-01 00:00:00
abstract::Human mesenchymal stromal cells (hMSCs) show great potential for clinical and experimental use due to their capacity to self-renew and differentiate into multiple mesenchymal lineages. However, disadvantages of primary cultures of hMSCs are the limited in vitro lifespan, and the variable properties of cells from diffe...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0599
更新日期:2014-10-01 00:00:00
abstract::To understand which growth factors/cytokines can affect migration of mesenchymal stem cells (MSCs) to injured tissues, we compared the effects of many (26) growth factors/cytokines on the migration activity of rabbit and human MSCs using a microchemotaxis chamber. Among them, platelet-derived growth factor (PDGF)-BB, ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.0032
更新日期:2007-02-01 00:00:00
abstract::To better understand the basis of variation in cellular reprogramming, we performed experiments with two primary objectives: first, to determine the degree of difference, if any, in reprogramming efficiency among cells lines of a similar type after accounting for technical variables, and second, to compare the efficie...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0040
更新日期:2013-10-01 00:00:00
abstract::Regenerative medicine is in demand of adult pluripotent stem cells (PSCs). The "Bonghan System (BHS)" was discovered and suggested to contain cells with regenerative capacity in the early 1960s. It had been ignored for a long time due to the lack of sufficient details of experiments, but about 37 years after the initi...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2015.0024
更新日期:2015-10-01 00:00:00
abstract::Lifelong neurogenesis in the mouse olfactory epithelium (OE) is regulated by the response of stem/progenitor cells to local signals, but embryonic and adult OE progenitors appear to be quite different--with potentially different mechanisms of regulation. A recently identified progenitor unique to embryonic OE--the nes...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0406
更新日期:2013-02-15 00:00:00
abstract::Non-tumorous liver tissue removed during surgery to resect hepatocellular carcinoma (HCC) is potentially a useful source of material from which cells, particularly liver progenitor/stem cells (LPCs), can be isolated to establish cell lines. The purpose of this study was to evaluate the applicability of the "plate-and-...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0304
更新日期:2010-08-01 00:00:00
abstract::PU.1 is a versatile hematopoietic cell-specific ETS-family transcriptional regulator required for the development of both the inborn and the adaptive immunity, owing to its potential ability to regulate the expression of multiple genes specific for different lineages during normal hematopoiesis. It functions in a cell...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2005.14.140
更新日期:2005-04-01 00:00:00