Abstract:
:Geminin is a multifunctional protein previously suggested to both maintain the bone morphogenetic protein inhibition required for neural induction and to control cell-cycle progression and cell fate in the early embryo. Since Geminin is required in the blastocyst on E3.5, we employed shRNA to examine its role during postimplantation development. Geminin knockdown inhibited the epithelial to mesenchymal transition (EMT) required at gastrulation and neural crest delamination, resulting in anterior-posterior axis and patterning defects, while overexpression promoted EMT at both locations. Geminin was negatively correlated with expression of E-cadherin, which is critically involved in controlling epithelial architecture. In addition, Geminin expression level was correlated with Wnt signaling and expression of the Wnt target gene Axin2 and with Msx2, and negatively correlated with the expression of Bmp4 and Neurog1 in quantitative reverse transcriptase-polymerase chain reaction analysis of RNAs from individual embryos. These results suggest that in addition to patterning the early embryo, Geminin plays a previously unrecognized role in EMT via its ability to affect Wnt signaling and E-cadherin expression.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Emmett LS,O'Shea KSdoi
10.1089/scd.2011.0483subject
Has Abstractpub_date
2012-09-01 00:00:00pages
2395-409issue
13eissn
1547-3287issn
1557-8534journal_volume
21pub_type
杂志文章abstract::Human stem cells from various sources have potential therapeutic applications. The clinical implementation of these therapies introduces the need for methods of noninvasive tracking of cells. The purpose of this study was to evaluate a high resolution magnetic resonance imaging (MRI) technique for in vivo detection an...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0028
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abstract::Amniotic fluid stem cells (AFSs) are interesting mesenchymal stem cells (MSCs) that are characterized by their great potential for cell proliferation and differentiation compared with other types of MSCs identified to date. However, MSCs in prolonged culture have been found to exhibit defects in genetic stability and ...
journal_title:Stem cells and development
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doi:10.1089/scd.2012.0371
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abstract::Non-tumorous liver tissue removed during surgery to resect hepatocellular carcinoma (HCC) is potentially a useful source of material from which cells, particularly liver progenitor/stem cells (LPCs), can be isolated to establish cell lines. The purpose of this study was to evaluate the applicability of the "plate-and-...
journal_title:Stem cells and development
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更新日期:2010-08-01 00:00:00
abstract::The mammalian inner ear is vulnerable to genetic disorders and aging, as well as to injuries caused by overstimulation, ototoxic drugs, and viral infections. Due to the poor regeneration of the sensory epithelium and the spiral ganglion neurons in the adult mammalian inner ear, cell replacement therapy strategies have...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2006.15.449
更新日期:2006-06-01 00:00:00
abstract::Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice and these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it is essential to unde...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0334
更新日期:2017-06-01 00:00:00
abstract::Previous studies have transplanted a variety of neural stem cells (NSCs) to the eye in hopes of developing a therapy to replace retinal neurons lost to disease. Successful integration, survival, and differentiation of the cell types has been variably successful. At the moment, little is known about the fundamental bio...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0051
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abstract::The paired type homeobox 6 (Pax6) transcription factor (TF) regulates multiple aspects of neural stem cell (NSC) and neuron development in the embryonic central nervous system. However, less is known about the role of Pax6 in the maintenance and differentiation of adult NSCs and in adult neurogenesis. Using the +/Sey(...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0058
更新日期:2014-12-01 00:00:00
abstract::In this retrospective study, 209 patients with malignant disease were analyzed for levels of T-cell receptor excision circles (TRECs) for the first 24 months after allogeneic stem cell transplantation. CD3(+) cells were separated by direct antibody-coupled magnetic beads, followed by DNA extraction according to a stan...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0588
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abstract::Osteophytes are a distinct feature of osteoarthritis (OA). Their formation may be related to pluripotential cells in the periosteum responding to stimulus during OA. This study aimed to isolate stem cells from osteophyte tissues and to characterize their phenotype, proliferation, and differentiation potential, as well...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0146
更新日期:2008-04-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2010.0277
更新日期:2011-07-01 00:00:00
abstract::Retinoic acid (RA) induces mouse F9 cells to form primitive endoderm (PrE) and increased levels of reactive oxygen species (ROS) accompany differentiation. ROS are obligatory for differentiation and while H2O2 alone induces PrE, antioxidants attenuate the response to RA. Evidence shows that ROS can modulate the Wnt/β-...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0010
更新日期:2014-05-15 00:00:00
abstract::N-methyl-d-aspartate receptors (NMDARs) are expressed abundantly in the brain and play an important role in the regulation of neuronal development, learning, memory, neurodegenerative diseases, and neurogenesis, all of which are extensively discussed in reviews published elsewhere. While the number of research article...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2016.0325
更新日期:2017-06-01 00:00:00
abstract::Multipotent mesenchymal stromal cells (MSCs) are more and more frequently used to treat orthopedic injuries in horses. However, these cells are limited in their expandability and differentiation capacity. Recently, the first equine-induced pluripotent stem cell (iPSC) lines were reported by us [ 1 ]. In vitro differen...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0409
更新日期:2016-01-01 00:00:00
abstract::The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor-based ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0048
更新日期:2017-11-01 00:00:00
abstract::The generation of Schwann cells from precursors within adult skin and bone marrow is of significant clinical interest because of the opportunities for disease modelling and strategies for remyelination. Recent evidence has suggested that glial cells can be generated from (i) mesenchymal stem cells (MSCs) within adult ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0371
更新日期:2010-07-01 00:00:00
abstract::Postnatal pancreas is a potential source for progenitor cells to generate endocrine β-cells for treating type 1 diabetes. However, it remains unclear whether young (1-week-old) pancreas harbors multipotent progenitors capable of differentiating into duct, acinar, and endocrine cells. Laminin is an extracellular matrix...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0007
更新日期:2015-09-01 00:00:00
abstract::There is an increasing need for an animal model that can be used to translate basic research into clinical therapy. We documented the differentiation and functional competence of embryonic stem cell (ESC)-derived endothelial cells in baboons. Baboon angioblasts were sequentially differentiated from embryoid body cultu...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0313
更新日期:2013-02-15 00:00:00
abstract::The capability to form all cell types of the body is a unique feature of stem cells. However, many questions remain concerning the mechanisms regulating differentiation potential. The derivation of spermatogonial cell lines (SGs) from mouse and human, which can differentiate across germ-layer borders, suggested male g...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0290
更新日期:2011-08-01 00:00:00
abstract::Ossification of the posterior longitudinal ligament (OPLL) is characterized by ectopic OPLL. To date, the specific molecular pathogenesis of OPLL has not been clearly elucidated. In this study, bone marrow-derived mesenchymal stem cells obtained from healthy donors (HD-MSCs) and patients with OPLL (OPLL-MSCs) were cul...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0117
更新日期:2020-12-01 00:00:00
abstract::Members of the homeobox (Hox) gene family are known to mediate expansion of hematopoietic stem cells (HSCs) and progenitors. The absence of oncogenic properties promoted HOXB4 as prime candidate in the quest to expand HSCs for clinical purposes. Despite its potential to expand HSCs, studies with mutant mice showed tha...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0259
更新日期:2012-01-01 00:00:00
abstract::We have isolated a novel progenitor cell population from adult rat pancreatic ducts, termed pancreatic-derived progenitor cells (PDPCs). Here, we report the in vitro culture, selection, and characterization of Thy1.1-positive and Thy1.1-negative PDPC subpopulations. These cells exhibit bipotentiality for differentiati...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0301
更新日期:2009-12-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0375
更新日期:2014-04-15 00:00:00
abstract::Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell prepar...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0265
更新日期:2011-04-01 00:00:00
abstract::The increasing use of mesenchymal stromal cells (MSC) in clinical cellular therapy requires a safe and controlled production process compliant with Good Manufacturing Practice guidelines. Pooled blood group AB human serum (HS) has been used to replace fetal bovine serum (FBS), critically rated by the regulatory agenci...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0051
更新日期:2013-03-01 00:00:00
abstract::In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0142
更新日期:2010-02-01 00:00:00
abstract::To better understand the basis of variation in cellular reprogramming, we performed experiments with two primary objectives: first, to determine the degree of difference, if any, in reprogramming efficiency among cells lines of a similar type after accounting for technical variables, and second, to compare the efficie...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0040
更新日期:2013-10-01 00:00:00
abstract::After intra-articular injection, synovium-derived mesenchymal stem cells (SMSCs) can adhere to damaged cartilage (a process called homing) and then repair the cartilage defect. Nonetheless, the main obstacle of the current method is the insufficient homing ratio of SMSCs, which fails to meet the requirements for carti...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2019.0137
更新日期:2020-04-01 00:00:00
abstract::We have previously isolated mesenchymal stromal cells (MSCs) from the tracheal aspirates of premature neonates with respiratory distress. Although isolation of MSCs correlates with the development of bronchopulmonary dysplasia, the physiologic role of these cells remains unclear. To address this, we further characteri...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0494
更新日期:2011-11-01 00:00:00
abstract::Special features of mesenchymal stem cells (MSCs) have made them a popular tool in cell therapy and tissue engineering. Although mouse animal models and murine MSCs are common tools in this field, our understanding of the effect of in vitro expansion on the behavior of these cells is poor and controversial. In additio...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0266
更新日期:2011-08-01 00:00:00
abstract::Oligodendrogenesis encompasses lineage specification of neural progenitor cells (NPCs) and differentiation into oligodendrocytes that ultimately culminates in the myelination of central nervous system axons. Each individual process must be tightly regulated by extracellular and cell-intrinsic mechanisms, whose identit...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0137
更新日期:2012-07-20 00:00:00