Abstract:
:Oligodendrogenesis encompasses lineage specification of neural progenitor cells (NPCs) and differentiation into oligodendrocytes that ultimately culminates in the myelination of central nervous system axons. Each individual process must be tightly regulated by extracellular and cell-intrinsic mechanisms, whose identities are barely understood. We had previously demonstrated that soluble factors derived from rat mesenchymal stem cells (MSCs) induce oligodendrogenesis in differentiating adult NPCs under differentiation conditions. However, since lineage specification predominantly occurs in proliferating progenitors and not necessarily during early differentiation, we investigated if soluble factors derived from MSCs are able to prime NPCs to the oligodendroglial fate already under proliferation conditions. Therefore, we analyzed the effects of a 3 weeks stimulation of adult NPCs under proliferation conditions with conditioned media derived from MSCs (MSC-CM) in terms of cell morphology, proliferation, cell-specific marker expression profile, response to growth factor withdrawal (GFW), cell-lineage restriction, and expression of glial fate determinants. While MSC-CM did not affect the proliferation rate of NPCs, it boosted the formation of 2', 3'-cyclic-nucleotide-3'-phosphodieesterase (CNPase)- and myelin basic protein-expressing oligodendrocytes after GFW, even when cells were exposed to an astrogenic milieu. Moreover, it reinforced the proper development of oligodendrocytes, since it ensured a sustained expression of the functional marker CNPase. Finally, the presence of MSC-CM reduced the anti-oligodendrogenic determinant Id2 in proliferating NPCs, thus increasing the relative proportion of the pro-oligodendrogenic factor Olig2 expression. In summary, MSCs prime proliferating progenitors and, thus, reinforce cell fate choice and accelerate differentiation toward the oligodendrocyte lineage. The present findings underscore the potential use of MSCs in cell therapies for remyelination such as in multiple sclerosis and spinal cord injury. Moreover, they urge the identification of the oligodendrogenic activity(ies) derived from MSCs to develop novel molecular therapies for demyelinating diseases.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Steffenhagen C,Dechant FX,Oberbauer E,Furtner T,Weidner N,Küry P,Aigner L,Rivera FJdoi
10.1089/scd.2011.0137subject
Has Abstractpub_date
2012-07-20 00:00:00pages
1838-51issue
11eissn
1547-3287issn
1557-8534journal_volume
21pub_type
杂志文章abstract::Improvement in angiogenesis using mesenchymal stem cells (MSCs) is evolving as an option in patients with vascular insufficiencies. The paracrine factors secreted by MSCs have been attributed to the angiogenic response. This study was conducted to identify the factors secreted by umbilical cord-derived MSCs (UCMSCs) t...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0184
更新日期:2015-02-15 00:00:00
abstract::Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs), and induced PSCs (iPSCs) are able to self-renew and differentiate into a multitude of specialized cellular lineages. In these cells, the pluripotential identity is maintained by a group of transcription factors (TFs). Among these factors, SOX TFs pl...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0297
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abstract::Embryonic stem (ES) cells are recognized as an excellent cell culture model for studying developmental mechanisms and their therapeutic modulations. The aim of this work was to define whether using magnetofection was an efficient way to manipulate stem cells genetically without adversely affecting their proliferation ...
journal_title:Stem cells and development
pub_type: 杂志文章
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abstract::In future, adipose-derived stem cells (ASC) might be used to treat neurological disorders. In this study, the neurotrophic and angiogenic properties of human ASC were evaluated, and their effects in a peripheral nerve injury model were determined. In vitro growth factor stimulation of the cells resulted in increased s...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0396
更新日期:2014-04-01 00:00:00
abstract::In the mammalian olfactory epithelium (OE), olfactory receptor neurons (ORNs) are continuously regenerated throughout the animal's lifetime. Horizontal basal cells (HBCs) in the OE express the epithelial marker keratin 5 (K5) and the stem cell marker Pax6 and are considered relatively quiescent tissue stem cells in th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0011
更新日期:2015-08-15 00:00:00
abstract::Halting the first clinical trial on the use of embryonic stem cell derivatives for spinal cord injury resulted in disappointment and created concerns about the future use of pluripotent stem cell-based therapy in the treatment of human diseases. This article presents reflections and concerns related to the halted embr...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0266
更新日期:2014-01-01 00:00:00
abstract::Cell replacement therapy holds great promise for treating a wide range of human disorders. However, ensuring the predictable differentiation of transplanted stem cells, eliminating their risk of tumor formation, and generating fully functional cells after transplantation remain major challenges in regenerative medicin...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0555
更新日期:2011-11-01 00:00:00
abstract::In spina bifida, the neural tube fails to close during the embryonic period. Exposure of the neural tube to the amniotic fluid during pregnancy causes additional neural damage. Intrauterine tissue engineering using a biomaterial seeded with stem cells might prevent this additional damage. For this purpose, autologous ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0334
更新日期:2014-03-01 00:00:00
abstract::Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice and these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it is essential to unde...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0334
更新日期:2017-06-01 00:00:00
abstract::On May 6th 2014, the European Court of Human Rights added yet a new element to the judicial history of stem cells as it ruled in Durisotto v. Italy [appeal n. 62804/13]. The ruling rejected a patient claim to access an unproven cell therapy-an outcome that is certainly to be welcomed. However, this ruling is a missed ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0361
更新日期:2014-12-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2006.15.575
更新日期:2006-08-01 00:00:00
abstract::Expression of the transcription factor Snail is required for normal vasculogenesis in the developing mouse embryo. In addition, tumors expressing Snail have been associated with a more malignant phenotype, with both increased invasive properties and angiogenesis. Although the relationship between Snail and vasculogene...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0194
更新日期:2012-01-20 00:00:00
abstract::Self-renewal and differentiation of endogenous stem cells (SCs) are essential for adult tissue homoeostasis and intrinsic healing capacity. In this study, we hypothesize that penis contains a small population of endogenous SCs, which might help rejuvenation of damaged erectile function. In this study, 60 newborn male ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0380
更新日期:2015-03-15 00:00:00
abstract::Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0138
更新日期:2010-06-01 00:00:00
abstract::There have been reports of marrow cells converting into pulmonary epithelial cells after marrow transplantation in irradiated mice. We evaluated the impact of whole bone marrow (WBM) infusion in mice, with or without total body irradiation (TBI), treated with saline or monocrotaline (MCT), which induces pulmonary hype...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0237
更新日期:2009-06-01 00:00:00
abstract::Fertility clinics worldwide routinely produce a large volume of 'waste' follicular aspirate, which is potentially an abundant source of immature ovarian follicles. Current attempts to cultivate these further in vitro to yield viable mature oocytes for fertility treatment have not yet achieved much success. Instead, re...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.11
更新日期:2005-02-01 00:00:00
abstract::We have isolated a novel progenitor cell population from adult rat pancreatic ducts, termed pancreatic-derived progenitor cells (PDPCs). Here, we report the in vitro culture, selection, and characterization of Thy1.1-positive and Thy1.1-negative PDPC subpopulations. These cells exhibit bipotentiality for differentiati...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0301
更新日期:2009-12-01 00:00:00
abstract::Studies on rodent bone marrow stromal cells (MSCs) have revealed a capacity, for at least a portion of cells, to express neuron-like traits after differentiation in culture. Little, however, is known about the ability of human MSCs in this regard. We show here that incubation with certain differentiation cocktails, pa...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2004.13.625
更新日期:2004-12-01 00:00:00
abstract::Lymphedema is a medically irreversible condition for which currently conservative and surgical therapies are either ineffective or impractical. The potential use of progenitor and stem cell-based therapies has offered a paradigm that may provide alternative treatment options for lymphatic disorders. Moreover, basic re...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0390
更新日期:2015-02-01 00:00:00
abstract::Electrical stimulation (ES) of cells has been shown to induce a variety of responses, such as cytoskeleton rearrangements, migration, proliferation, and differentiation. In this study, we have investigated whether monophasic and biphasic pulsed ES could exert any effect on the proliferation and differentiation of huma...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0375
更新日期:2014-04-15 00:00:00
abstract::CD41 (αIIb integrin), a specific marker for megakaryocytes and platelets, was recently shown to mark the initiation of definitive hematopoiesis in mouse embryos. However, whether embryonic CD41(+) populations have a nonhematopoietic potential remains elusive. Here, we report that the CD41(+) cells from the mouse E11.0...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0572
更新日期:2012-09-20 00:00:00
abstract::The regenerative capacity of the endometrium has been attributed to resident stem/progenitor cells. A number of stem/progenitor markers have been reported for human endometrial stem/progenitor cells; however, the lack of convenient markers in the mouse has made experimental investigation into endometrial regeneration ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2018.0133
更新日期:2019-01-01 00:00:00
abstract::Misexpression of the double homeodomain protein DUX4 in muscle is believed to cause facioscapulohumeral muscular dystrophy (FSHD). Although strategies are being devised to inhibit DUX4 activity in FSHD, there is little known about the normal function of this protein. Expression of DUX4 has been reported in pluripotent...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0643
更新日期:2013-09-01 00:00:00
abstract::Human mesenchymal stem cells (MSCs) are a promising tool for therapeutic applications in cell-based therapy and regenerative medicine, and MSCs from the human palatine tonsils have recently been used as a new tissue source. However, the understanding of the proliferation and differentiation capacity of tonsil-derived ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2016.0061
更新日期:2016-08-01 00:00:00
abstract::Amniotic fluid stem cells (AFSs) are interesting mesenchymal stem cells (MSCs) that are characterized by their great potential for cell proliferation and differentiation compared with other types of MSCs identified to date. However, MSCs in prolonged culture have been found to exhibit defects in genetic stability and ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0371
更新日期:2013-05-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) have a superior immunomodulatory capacity compared to other cells of the immune system, and they hold great promise for treating various immune disorders. However, their regulatory effects on the maturation of immature dendritic cells (imDCs) are not fully understood. In this study, we sh...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0235
更新日期:2018-04-15 00:00:00
abstract::The AMP-activated protein kinase (AMPK) mediates rapid, stress-induced loss of the inhibitor of differentiation (Id)2 in blastocysts and trophoblast stem cells (TSC), and a lasting differentiation in TSC. However, it is not known if AMPK regulates other potency factors or regulates them before the blastocyst stage. Th...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0352
更新日期:2013-05-15 00:00:00
abstract::Stem cell factor (SCF) stimulation of the receptor tyrosine kinase c-kit has effects on the proliferation, differentiation, and apoptotic regulation of hematopoietic progenitor cell populations. Rat bone marrow myelomonocytic stem cells (MSC) isolated in vitro by wheat germ agglutinin culture exclusively undergo self-...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2005.14.556
更新日期:2005-10-01 00:00:00
abstract::The mechanisms responsible for the inhibitory effects of mesenchymal stem cells (MSCs) on dendritic cells (DCs) are still poorly understood. Our investigation of the potential signaling pathways revealed for the first time that human umbilical-cord-derived MSCs (UC-MSCs) instruct DCs to acquire tolerogenic phenotypes ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0559
更新日期:2014-09-01 00:00:00
abstract::The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0262
更新日期:2009-01-01 00:00:00