Abstract:
:Hepatic ischemia reperfusion injury (IRI) remains a major obstacle in liver resection and transplantation surgery, especially in diseased organs. Human mesenchymal stromal cells (MSCs) are reported to acutely alleviate hepatic IRI in mice by releasing bioactive membrane-enclosed extracellular vesicles (EVs), but the long-term effects of MSC-derived EV on hepatic IRI are unknown. Given the considerable differentiation capacity of fibroblasts (FBs) during wound healing and their morphological similarities with MSC, the present study aimed to investigate the potential of these two cell types and their cell-derived EV in attenuating liver damage after IRI. EVs were isolated and purified from the supernatant of MSC and FB cultures and, subsequently, characterized by electron microscopy, nanoparticle tracking analysis, and western blot. Liver injury and organ regeneration in a murine in vivo model of IRI were assessed by serum transaminase levels, histopathology, and immunohistochemistry. Changes in expression of inflammation-associated genes within liver tissue were evaluated by reverse transcriptase quantitative polymerase chain reaction. MSC, MSC-derived EV, FB, and FB-derived EV were systemically administered before hepatic IRI. We found that MSC and MSC-derived EV decreased serum transaminase levels, reduced hepatic necrosis, increased the amount of Ki67-positive hepatocytes, and repressed the transcription of inflammation-associated genes. Although they had no impact on organ damage, FB and FB-derived EV showed some regenerative potential in the late phase of hepatic IRI. Compared to FB, MSC and their derived EV had a stronger potential to attenuate liver damage and improve organ regeneration after hepatic IRI. These results suggest that the key therapeutic factors are located within EV.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Anger F,Camara M,Ellinger E,Germer CT,Schlegel N,Otto C,Klein Idoi
10.1089/scd.2019.0085subject
Has Abstractpub_date
2019-11-01 00:00:00pages
1451-1462issue
21eissn
1547-3287issn
1557-8534journal_volume
28pub_type
杂志文章abstract::Immunodeficient mice transplanted with human hematopoietic stem cells (HSCs) have been referred to as "Human Immune System" (HIS) mice and are a translational platform for studying human immune responses in vivo. Human HSC sources used in generating HIS mice include fetal liver (FL), umbilical cord blood (CB), and adu...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0156
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abstract::CD41 (αIIb integrin), a specific marker for megakaryocytes and platelets, was recently shown to mark the initiation of definitive hematopoiesis in mouse embryos. However, whether embryonic CD41(+) populations have a nonhematopoietic potential remains elusive. Here, we report that the CD41(+) cells from the mouse E11.0...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0572
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abstract::The mechanisms responsible for the inhibitory effects of mesenchymal stem cells (MSCs) on dendritic cells (DCs) are still poorly understood. Our investigation of the potential signaling pathways revealed for the first time that human umbilical-cord-derived MSCs (UC-MSCs) instruct DCs to acquire tolerogenic phenotypes ...
journal_title:Stem cells and development
pub_type: 杂志文章
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abstract::Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for address...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0174
更新日期:2021-02-01 00:00:00
abstract::On May 6th 2014, the European Court of Human Rights added yet a new element to the judicial history of stem cells as it ruled in Durisotto v. Italy [appeal n. 62804/13]. The ruling rejected a patient claim to access an unproven cell therapy-an outcome that is certainly to be welcomed. However, this ruling is a missed ...
journal_title:Stem cells and development
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doi:10.1089/scd.2014.0361
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abstract::The design of reliable biocompatible and biodegradable scaffolds remains one of the most important challenges for tissue engineering. In fact, properly designed scaffolds must display an adequate and interconnected porosity to facilitate cell spreading and colonization of the inner layers, and must release physical si...
journal_title:Stem cells and development
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doi:10.1089/scd.2017.0051
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abstract::Bone marrow-mesenchymal stem cells (BM-MSC) ameliorate renal dysfunction and repair tubular damage of acute kidney injury by locally releasing growth factors, including the insulin-like growth factor-1 (IGF-1). The restricted homing of BM-MSC at the site of injury led us to investigate a possible gene-based communicat...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0266
更新日期:2013-03-01 00:00:00
abstract::Esophagus and trachea arise from a common origin, the anterior foregut tube. The compartmentalization process of the foregut into the esophagus and trachea is still poorly understood. Esophageal atresia/tracheoesophageal fistula (EA/TEF) is one of the most common gastrointestinal congenital defects with an incidence r...
journal_title:Stem cells and development
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doi:10.1089/scd.2020.0075
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abstract::The corneal endothelium is believed to be developmentally originated from the periocular mesenchyme via the neural crest. Human corneal endothelial progenitor cells (HCEPs) have been investigated because of their potential availability for the tissue regenerative medicine. However, the existence and the properties of ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0387
更新日期:2014-09-15 00:00:00
abstract::Special features of mesenchymal stem cells (MSCs) have made them a popular tool in cell therapy and tissue engineering. Although mouse animal models and murine MSCs are common tools in this field, our understanding of the effect of in vitro expansion on the behavior of these cells is poor and controversial. In additio...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2010.0266
更新日期:2011-08-01 00:00:00
abstract::A common observation in metabolic disorders and aging is the elevation of free fatty acids, which can form ectopic fat deposition and result in lipotoxicity. Ectopic fat deposition of skeletal muscle has been recognized as an important component of aging, frailty and sarcopenia. Previous studies have suggested that li...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2020.0168
更新日期:2021-01-11 00:00:00
abstract::The difficulty in long-term expansion of mesenchymal stem cells (MSCs) using standard culture systems without the loss of their stem cell properties suggests that a critical feature of their microenvironment necessary for retention of stem cell properties is absent in these culture systems. We report here the reconsti...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0217
更新日期:2010-07-01 00:00:00
abstract::There is surprising confusion surrounding the concept of biological totipotency, both within the scientific community and in society at large. Increasingly, ethical objections to scientific research have both practical and political implications. Ethical controversy surrounding an area of research can have a chilling ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0364
更新日期:2014-04-15 00:00:00
abstract::Spiral ganglion neurons (SGNs) are usually damaged in sensorineural hearing loss. SGN-derived neural stem cells (NSCs) have been identified and proposed to differentiate into neurons to replace damaged SGNs. However, it remains obscure whether SGN-NSC-derived neurons (ScNs) are electrophysiologically functional and po...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2015.0345
更新日期:2016-05-15 00:00:00
abstract::Retinoic acid (RA) is present at sites of neurogenesis in both the embryonic and adult brain. While it is widely accepted that RA signaling is involved in the regulation of neural stem cell differentiation, little is known about vitamin A utilization and biosynthesis of active retinoids in the neurogenic niches, or ab...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2012.0422
更新日期:2013-10-15 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2013.0588
更新日期:2014-07-15 00:00:00
abstract::Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. In this study, we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from random positioning machine (...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2017.0262
更新日期:2018-06-15 00:00:00
abstract::Myocardial infarction (MI) results in loss of myofibers in the ischemic zone of the heart, followed by scar formation. These factors increase barriers to mobilization of mesenchymal stem cells (MSC), thereby impeding their effectiveness in cardiac repair. This study examined MSC overexpressing CXCR4 (MSC(CX4)) to dete...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2011.0126
更新日期:2012-03-20 00:00:00
abstract::Betacellulin (BTC), a ligand of the epidermal growth factor receptor, has been shown to promote growth and differentiation of pancreatic β-cells and to improve glucose metabolism in experimental diabetic rodent models. Mesenchymal stem cells (MSCs) have been already proved to be multipotent. Recent work has attributed...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0490
更新日期:2011-02-01 00:00:00
abstract::In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GF...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0142
更新日期:2010-02-01 00:00:00
abstract::Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts fro...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.0032
更新日期:2007-10-01 00:00:00
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journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0520
更新日期:2010-09-01 00:00:00
abstract::Aneurysm refers to the dilation of the vessel wall for more than 50%. Abdominal aortic aneurysm (AAA) refers to the dilation and weakening of all three layers of the abdominal aorta, which mostly occur infrarenally. The population aged above 50 years is at risk of AAA development, while a familiar history doubles the ...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2014.0517
更新日期:2015-07-01 00:00:00
abstract::Over the past 20 months, reports claiming the generation of induced pluripotent stem (iPS) cells with characteristics identical to those of embryonic stem (ES) cells from nonembryonic tissue have captured great attention in both the scientific community and the general public. In the light of the continuing controvers...
journal_title:Stem cells and development
pub_type: 杂志文章,评审
doi:10.1089/scd.2008.0062
更新日期:2008-06-01 00:00:00
abstract::Resistance to imatinib can occur in patients with chronic myelogenous leukemia (CML). In this study, we report mechanisms of action of histone deacetylase (HDAC) inhibitor, depsipeptide (FK228) in BCR/ABL-expressing cell lines and its effectiveness in imatinib-resistant cells from patients with blast crisis of CML. FK...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2007.9994
更新日期:2007-06-01 00:00:00
abstract::True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on a...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0124
更新日期:2014-08-15 00:00:00
abstract::Allergic asthma is an inflammatory airway disease caused by T helper type 2 (Th2)-driven immune responses. Recent studies have demonstrated that adipose-derived stromal cells (ASC) have an immunosuppressive effect on T-cell activity. This study was performed to investigate whether ASC can inhibit Th2-dependent allergi...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2009.0513
更新日期:2010-11-01 00:00:00
abstract::There have been reports of marrow cells converting into pulmonary epithelial cells after marrow transplantation in irradiated mice. We evaluated the impact of whole bone marrow (WBM) infusion in mice, with or without total body irradiation (TBI), treated with saline or monocrotaline (MCT), which induces pulmonary hype...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2008.0237
更新日期:2009-06-01 00:00:00
abstract::Self-renewal and differentiation of endogenous stem cells (SCs) are essential for adult tissue homoeostasis and intrinsic healing capacity. In this study, we hypothesize that penis contains a small population of endogenous SCs, which might help rejuvenation of damaged erectile function. In this study, 60 newborn male ...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0380
更新日期:2015-03-15 00:00:00
abstract::Human embryonic stem cells (hESCs) exhibiting skewed X chromosome inactivation (XCI) have been reported. The copy number variations (CNVs), loss of heterozygosity (LOH), or single-nucleotide variant (SNV) events in those epigenetically distinct cells remain unknown, and whether such genetic abnormalities will influenc...
journal_title:Stem cells and development
pub_type: 杂志文章
doi:10.1089/scd.2014.0463
更新日期:2015-08-01 00:00:00