Abstract:
:The induction and detection of chemotactic migration in mesenchymal stem cells (MSC) are complicated by their adherent nature. We describe here the experimental details for inducing the transmigration of MSC seeded in microchemotaxis chambers and reliably identifying the translocated cells. A combination of 8-microm pore-sized membranes and a two-step staining procedure resulted in the detection of MSC migration against a gradient of human plasma which plateaued after 4 h. Microscopic enumeration of the transmigrated cells clearly distinguished contaminating nonmigrated cells from transmigrated cells. Chemokinetic and chemotactic effects could be separated and were influenced by precoated fibronectin or vitronectin. Thus, the technique described allows rapid and reliable induction and determination of migration in adherent MSC.
journal_name
Stem Cells Devjournal_title
Stem cells and developmentauthors
Rüster B,Grace B,Seitz O,Seifried E,Henschler Rdoi
10.1089/scd.2005.14.231keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
231-5issue
2eissn
1547-3287issn
1557-8534journal_volume
14pub_type
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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journal_title:Stem cells and development
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