Myosin IC generates power over a range of loads via a new tension-sensing mechanism.

abstract：:This work argues that, in a polarized environment, scientists can minimize the likelihood that the audience's biased processing will lead to rejection of their message if they not only eschew advocacy but also, convey that they are sharers of knowledge faithful to science's way of knowing and respectful of the audienc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamieson KH,Hardy BW

更新日期：2014-09-16 00:00:00

abstract：:The DNA in chromatin of isolated thymus nuclei, and in two different preparations of deoxyribonucleoprotein extracted from chromatin, has been digested with DNase. By variation of the time of reaction and the concentration of enzyme, and by comparison of the action of the enzyme on (protein-free) DNA with its action o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mirsky AE

更新日期：1971-12-01 00:00:00

abstract：:Fodrin, a protein composed of two polypeptides with molecular weights of 250,000 and 240,000, is concentrated in the cortical cytoplasm of neurons, and moves down the axons by the process of axonal transport. We have used immunofluorescence techniques to determine whether fodrin antigens also move in non-neuronal cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine J,Willard M

更新日期：1983-01-01 00:00:00

abstract：:One of the earliest detectable events during plant-pathogen interaction is a rapid increase in ethylene biosynthesis. This gaseous plant stress hormone may be a signal for plants to activate defense mechanisms against invading pathogens such as bacteria, fungi, and viruses. The effect of ethylene on four plant genes i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ecker JR,Davis RW

更新日期：1987-08-01 00:00:00

abstract：:A form of two-dimensional (2D) vibrational spectroscopy, which uses two ultrafast IR laser pulses, is used to examine the structure of a cyclic penta-peptide in solution. Spectrally resolved cross peaks occur in the off-diagonal region of the 2D IR spectrum of the amide I region, analogous to those in 2D NMR spectrosc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamm P,Lim M,DeGrado WF,Hochstrasser RM

更新日期：1999-03-02 00:00:00

abstract：:The mRNA for organic anion transport protein (oatp) was previously shown to be present in abundance in liver and kidney, and in small amounts in brain. Data obtained from experiments with reverse transcriptase-PCR techniques and in situ hybridization analysis showed that the oatp mRNA is present within the brain, loca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Angeletti RH,Novikoff PM,Juvvadi SR,Fritschy JM,Meier PJ,Wolkoff AW

更新日期：1997-01-07 00:00:00

abstract：:Adenine-thymine (AT)-rich interactive domain 5a (Arid5a) is an RNA-binding protein found in the cytoplasm and nucleus of normally growing cells. Although Arid5a is known to play an important role in immune regulation, whether and how Arid5a subcellular localization impacts immune regulation has remained unclear. In th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higa M,Oka M,Fujihara Y,Masuda K,Yoneda Y,Kishimoto T

更新日期：2018-02-06 00:00:00

abstract：:Theory predicts that double-core-hole (DCH) spectroscopy can provide a new powerful means of differentiating between similar chemical systems with a sensitivity not hitherto possible. Although DCH ionization on a single site in molecules was recently measured with double- and single-photon absorption, double-core hole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berrah N,Fang L,Murphy B,Osipov T,Ueda K,Kukk E,Feifel R,van der Meulen P,Salen P,Schmidt HT,Thomas RD,Larsson M,Richter R,Prince KC,Bozek JD,Bostedt C,Wada S,Piancastelli MN,Tashiro M,Ehara M

更新日期：2011-10-11 00:00:00

abstract：:The Hippo pathway is an evolutionarily conserved regulator of organ growth and tumorigenesis. In Drosophila, oncogenic RasV12 cooperates with loss-of-cell polarity to promote Hippo pathway-dependent tumor growth. To identify additional factors that modulate this signaling, we performed a genetic screen utilizing the D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo X,Sun Y,Azad T,Janse van Rensburg HJ,Luo J,Yang S,Liu P,Lv Z,Zhan M,Lu L,Zhou Y,Ma X,Zhang X,Yang X,Xue L

更新日期：2020-12-01 00:00:00

abstract：:Murine mucopolysaccharidosis type VII is a heritable disease caused by a spontaneous mutation, gus(mps), closely linked to the beta-glucuronidase structural gene on chromosome 5. Mice homozygous for the mutation have a > 200-fold decrease in beta-glucuronidase mRNA levels and virtually no enzyme activity detectable by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sands MS,Birkenmeier EH

更新日期：1993-07-15 00:00:00

abstract：:Type IV pili (Tfp) are highly conserved macromolecular structures that fulfill diverse cellular functions, such as adhesion to host cells, the import of extracellular DNA, kin recognition, and cell motility (twitching). Outstandingly, twitching motility enables a poorly understood process by which highly coordinated g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mercier R,Bautista S,Delannoy M,Gibert M,Guiseppi A,Herrou J,Mauriello EMF,Mignot T

更新日期：2020-11-10 00:00:00

abstract：:The three-dimensional structures of avian H5 and swine H9 influenza hemagglutinins (HAs) from viruses closely related to those that caused outbreaks of human disease in Hong Kong in 1997 and 1999 were determined bound to avian and human cell receptor analogs. Emerging influenza pandemics have been accompanied by the e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ha Y,Stevens DJ,Skehel JJ,Wiley DC

更新日期：2001-09-25 00:00:00

abstract：:A core operation in speech production is the preparation of words from a semantic base. The theory of lexical access reviewed in this article covers a sequence of processing stages beginning with the speaker's focusing on a target concept and ending with the initiation of articulation. The initial stages of preparatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levelt WJ

更新日期：2001-11-06 00:00:00

abstract：:The developmental plasticity of plants relies on the remarkable ability of the meristems to integrate nutrient and energy availability with environmental signals. Meristems in root and shoot apexes share highly similar molecular players but are spatially separated by soil. Whether and how these two meristematic tissue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Cai W,Liu Y,Li H,Fu L,Liu Z,Xu L,Liu H,Xu T,Xiong Y

更新日期：2017-03-07 00:00:00

abstract：:Integral feedback control is a basic engineering strategy for ensuring that the output of a system robustly tracks its desired value independent of noise or variations in system parameters. In biological systems, it is common for the response to an extracellular stimulus to return to its prestimulus value even in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yi TM,Huang Y,Simon MI,Doyle J

更新日期：2000-04-25 00:00:00

abstract：:Poly(A)-containing HeLa cell mRNA prepared from cells labeled with [methyl-(3)H]methionine or [(32)P]phosphate was found to contain a variety of methylated, blocked 5'-terminal structures of two general types: m(7)GpppN(7)-Np and m(7)GpppN(m)-N(m)-Np. In addition, about one-third of the [(3)H]methyl label was present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furuichi Y,Morgan M,Shatkin AJ,Jelinek W,Salditt-Georgieff M,Darnell JE

更新日期：1975-05-01 00:00:00

abstract：:Retrotransposons and retroviruses integrate nonrandomly into eukaryotic genomes. For the yeast retrotransposon Ty5, integration preferentially occurs within domains of heterochromatin. Targeting to these locations is determined by interactions between an amino acid sequence motif at the C terminus of Ty5 integrase (IN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu Y,Dai J,Fuerst PG,Voytas DF

更新日期：2003-05-13 00:00:00

abstract：:Solar photovoltaic (PV) electricity generation is expanding rapidly in China, with total capacity projected to be 400 GW by 2030. However, severe aerosol pollution over China reduces solar radiation reaching the surface. We estimate the aerosol impact on solar PV electricity generation at the provincial and regional g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Wagner F,Peng W,Yang J,Mauzerall DL

更新日期：2017-11-07 00:00:00

abstract：:Two of the most important models to account for the specificity and sensitivity of the T cell receptor (TCR) are the kinetic proofreading and serial ligation models. However, although kinetic proofreading provides a means for individual TCRs to measure accurately the length of time they are engaged and signal appropri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan C,George AJ,Stark J

更新日期：2001-05-08 00:00:00

abstract：:Escherichia coli DnaK acts as a molecular chaperone through its ATP-regulated binding and release of polypeptide substrates. Overexpressing a C-terminal fragment (CTF) of DnaK (Gly-384 to Lys-638) containing the polypeptide substrate binding domain is lethal in wild-type E. coli. This dominant-negative phenotype may r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burkholder WF,Zhao X,Zhu X,Hendrickson WA,Gragerov A,Gottesman ME

更新日期：1996-10-01 00:00:00

abstract：:Bone morphogenetic protein 4 (BMP-4) induces ventral mesoderm but represses dorsal mesoderm formation in Xenopus embryos. We show that BMP-4 inhibits two signaling pathways regulating dorsal mesoderm formation, the induction of dorsal mesoderm (Spemann organizer) and the dorsalization of ventral mesoderm. Ectopic expr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Re'em-Kalma Y,Lamb T,Frank D

更新日期：1995-12-19 00:00:00

abstract：:We present a simple theory that uses thermodynamic parameters to predict the probability that a protein retains the wild-type structure after one or more random amino acid substitutions. Our theory predicts that for large numbers of substitutions the probability that a protein retains its structure will decline expone...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bloom JD,Silberg JJ,Wilke CO,Drummond DA,Adami C,Arnold FH

更新日期：2005-01-18 00:00:00

abstract：:It has been shown that the pituitary contains a cytotropic factor (CTF) that stimulates the secretion of catecholamines by dopaminergic neurons of the hypothalamus. In the present study, CTF was purified from rat pituitaries and found by means of mass spectrometric analysis to be adenosine. This finding was corroborat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porter JC,Ijames CF,Wang TC,Markey SP

更新日期：1995-06-06 00:00:00

abstract：:The apoprotein corresponding to the mammalian photoreceptor rhodopsin has been expressed by using suspension cultures of HEK293S cells in defined media that contained 6-15N-lysine and 2-13C-glycine. Typical yields were 1.5-1.8 mg/liter. Incorporation of 6-15N-lysine was quantitative, whereas that of 2-13C-glycine was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eilers M,Reeves PJ,Ying W,Khorana HG,Smith SO

更新日期：1999-01-19 00:00:00

abstract：:HIV-1 infection in the brain induces neuronal apoptosis leading to HIV-associated dementia. To explore the underlying mechanism, we developed a murine model by using human peripheral blood mononuclear cell (PBMC)-transplanted nonobese diabetic (NOD)-severe combined immunodeficiency (SCID) (hu-PBMC-NOD-SCID) mice. Admi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miura Y,Misawa N,Kawano Y,Okada H,Inagaki Y,Yamamoto N,Ito M,Yagita H,Okumura K,Mizusawa H,Koyanagi Y

更新日期：2003-03-04 00:00:00

abstract：:The effect of metabolic depletion on the maturation-associated loss of membrane functions has been studied by using sheep reticulocytes incubated in vitro at 37 degrees C for periods up to 41 hr. ATP was either maintained with glucose, adenosine plus inosine, or depleted with 2-deoxyglucose plus arsenate. Two membrane...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weigensberg AM,Blostein R

更新日期：1983-08-01 00:00:00

abstract：:Spantide (D-Arg1-Pro2-Lys3-Pro4-Gln5-Gln6-D-Trp7-Phe8-D-Trp9-++ +Leu10-Leu11-NH2) was introduced as a tachykinin antagonist in 1984 and has served as a starting point in the design of new antagonists that have proven to be more effective and have exhibited no neurological side effects. The most remarkable and unpredic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Folkers K,Feng DM,Asano N,Håkanson R,Weisenfeld-Hallin Z,Leander S

更新日期：1990-06-01 00:00:00

abstract：:The nucleosomal repeat lengths of bulk chromatin and the chromatin of transcriptionally active and inactive genes were analyzed in two mouse cell lines and adult mouse spleens. The adult beta-globin gene exhibits a nucleosomal repeat length approximately 11 base pairs longer than (i) an inactive embryonic globin gene,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith RD,Seale RL,Yu J

更新日期：1983-09-01 00:00:00

abstract：:The activation of additional promoter sites by production of an alternative sigma subunit for RNA polymerase is a common strategy for the coordinate regulation of gene expression. Many alternative sigma factors control genes for specialized, and often narrowly distributed, functions. For example, most of the alternati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YF,Helmann JD

更新日期：1992-06-01 00:00:00

abstract：:Murine mammary tumor virus (MMTV) expression is analyzed in a T-lymphoid cell line (T1M1) sensitive to the killing effect of glucocorticoids and in two of its variants, one resistant (T1M1r) and one supersensitive (T1M1ss) to glucocorticoid-induced lymphocytolysis. In the T1M1 line, MMTV is expressed and induced appro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mermod JJ,Bourgeois S,Defer N,Crépin M

更新日期：1983-01-01 00:00:00