Abstract:
:Type IV pili (Tfp) are highly conserved macromolecular structures that fulfill diverse cellular functions, such as adhesion to host cells, the import of extracellular DNA, kin recognition, and cell motility (twitching). Outstandingly, twitching motility enables a poorly understood process by which highly coordinated groups of hundreds of cells move in cooperative manner, providing a basis for multicellular behaviors, such as biofilm formation. In the social bacteria Myxococcus xanthus, we know that twitching motility is under the dependence of the small GTPase MglA, but the underlying molecular mechanisms remain elusive. Here we show that MglA complexed to GTP recruits a newly characterized Tfp regulator, termed SgmX, to activate Tfp machines at the bacterial cell pole. This mechanism also ensures spatial regulation of Tfp, explaining how MglA switching provokes directional reversals. This discovery paves the way to elucidate how polar Tfp machines are regulated to coordinate multicellular movements, a conserved feature in twitching bacteria.
journal_name
Proc Natl Acad Sci U S Aauthors
Mercier R,Bautista S,Delannoy M,Gibert M,Guiseppi A,Herrou J,Mauriello EMF,Mignot Tdoi
10.1073/pnas.2002783117subject
Has Abstractpub_date
2020-11-10 00:00:00pages
28366-28373issue
45eissn
0027-8424issn
1091-6490pii
2002783117journal_volume
117pub_type
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