A molecular phylogeny of the marine red algae (Rhodophyta) based on the nuclear small-subunit rRNA gene.

abstract：:Preferential phosphorylation of specific proteins by cAMP-dependent protein kinase (PKA) may be mediated in part by the anchoring of PKA to a family of A-kinase anchor proteins (AKAPs) positioned in close proximity to target proteins. This interaction is thought to depend on binding of the type II regulatory (RII) sub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burton KA,Johnson BD,Hausken ZE,Westenbroek RE,Idzerda RL,Scheuer T,Scott JD,Catterall WA,McKnight GS

更新日期：1997-09-30 00:00:00

abstract：:Cytochrome P450 enzymes are monooxygenases that contain a functional heme b group linked to a conserved cysteine with a thiolate bond. In the native state, the central iron atom is hexacoordinated with a covalently bound water molecule. The exclusion of solvent molecules from the active site is essential for efficient...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oprea TI,Hummer G,Garcia AE

更新日期：1997-03-18 00:00:00

abstract：:For the design of potent subunit vaccines, it is of paramount importance to identify all antigens immunologically recognized by a patient population infected with a pathogen. We have developed a rapid and efficient procedure to identify such commonly recognized antigens, and here we provide a comprehensive in vivo ant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Etz H,Minh DB,Henics T,Dryla A,Winkler B,Triska C,Boyd AP,Söllner J,Schmidt W,von Ahsen U,Buschle M,Gill SR,Kolonay J,Khalak H,Fraser CM,von Gabain A,Nagy E,Meinke A

更新日期：2002-05-14 00:00:00

abstract：:We have reported that normal human salivary gland-derived epithelial cells exclusively express keratinocyte growth factor receptor (KGFR). In the process of malignant transformation of human salivary gland tumors, KGFR gene expression disappeared concomitantly with the de novo expression of the fibroblast growth facto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Wang H,Toratani S,Sato JD,Kan M,McKeehan WL,Okamoto T

更新日期：2001-09-25 00:00:00

abstract：:We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1-->2)[alpha-D-Abe(1-->3)]alpha-D-Manp1-->OMe, where Abe is abequose. The scFv expressed in Escherichia col...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zdanov A,Li Y,Bundle DR,Deng SJ,MacKenzie CR,Narang SA,Young NM,Cygler M

更新日期：1994-07-05 00:00:00

abstract：:Intense proteolysis of cytoskeletal proteins occurs in brain within minutes of transient ischemia, possibly because of the activation of calcium-sensitive proteases (calpains). This proteolytic event precedes overt signs of neuronal degeneration, is most pronounced in regions of selective neuronal vulnerability, and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee KS,Frank S,Vanderklish P,Arai A,Lynch G

更新日期：1991-08-15 00:00:00

abstract：:In order to assess whether DNA rearrangement occurs with equal frequency at each of the several J (joining region) elements in the mouse kappa light chain locus, we set out to determine the relative frequency of usage of J kappa segments in populations of B lymphocytes unperturbed by antigenic selection or cloning. To...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood DL,Coleclough C

更新日期：1984-08-01 00:00:00

abstract：:The adult repertoire of antibody specificities is acquired in a developmentally programmed fashion that, in mouse and man, parallels the ordered rearrangement of a limited number of germ-line heavy chain variable region (VH) gene segments during development. It has been hypothesized that this developmental bias is a c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willems van Dijk K,Milner LA,Sasso EH,Milner EC

更新日期：1992-11-01 00:00:00

abstract：:The nonlinear nature of integration among cortical brain areas renders the effective connectivity between them inherently dynamic and context-sensitive. One emerging architectural principle of functional brain organization, which rests explicitly on these nonlinear interactions, is that neuronal responses expressed at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friston KJ,Büchel C

更新日期：2000-06-20 00:00:00

abstract：:We have previously linked aging, carcinogenesis, and de novo methylation within the promoter of the estrogen receptor (ER) gene in human colon. We now examine the dynamics of this process for the imprinted gene for insulin-like growth factor II (IGF2). In young individuals, the P2-4 promoters of IGF2 are methylated ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Issa JP,Vertino PM,Boehm CD,Newsham IF,Baylin SB

更新日期：1996-10-15 00:00:00

abstract：:Nascent replicative form type II (RFII) DNA of coliphage M13 synthesized in an Escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith DNA polymerase I contains ribonucleotides that are retained in the covalently closed RFI DNA sealed in vitro by the joint action of T5 phage DNA polymerase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dasgupta S,Mitra S

更新日期：1978-01-01 00:00:00

abstract：:Treatment of cultured Hodgkin disease (HD) cells with neuraminidase results in decreased reactivity of monoclonal antibody VIM-D5 with its antigen, the X hapten, a fucosyl-N-acetyllactosamine. The other feature characteristic of HD cells is the expression of high levels of ectosialyltransferase activity. We present ev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paietta E,Stockert RJ,Morell AG,Diehl V,Wiernik PH

更新日期：1986-05-01 00:00:00

abstract：:Agrobacterium tumefaciens can transfer part of its Ti plasmid, the T-DNA, to plant cells where it integrates into the nuclear genome via illegitimate recombination. Integration of the T-DNA results in small deletions of the plant target DNA, and may lead to truncation of the T-DNA borders and the production of filler ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bundock P,Hooykaas PJ

更新日期：1996-12-24 00:00:00

abstract：:We have shown that the silk fibroin gene from Bombyx mori is faithfully transcribed in an in vitro transcription system of the HeLa cell extract prepared by the method of Manley et al. [Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. L. (1980) Proc. Natl. Acad. Sci. USA 77, 3855-3859]. Using this system a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsujimoto Y,Hirose S,Tsuda M,Suzuki Y

更新日期：1981-08-01 00:00:00

abstract：:The development of new antimicrobial drugs is a priority to combat the increasing spread of multidrug-resistant bacteria. This development is especially problematic in gram-negative bacteria due to the outer membrane (OM) permeability barrier and multidrug efflux pumps. Therefore, we screened for compounds that target...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hart EM,Mitchell AM,Konovalova A,Grabowicz M,Sheng J,Han X,Rodriguez-Rivera FP,Schwaid AG,Malinverni JC,Balibar CJ,Bodea S,Si Q,Wang H,Homsher MF,Painter RE,Ogawa AK,Sutterlin H,Roemer T,Black TA,Rothman DM,Walker

更新日期：2019-10-22 00:00:00

abstract：:beta-catenin has functions as both an adhesion and a signaling molecule. Disruption of these functions through mutations of the beta-catenin gene (CTNNB1) may be important in the development of colorectal tumors. We examined the entire coding sequence of beta-catenin by reverse transcriptase-PCR (RT-PCR) and direct se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ilyas M,Tomlinson IP,Rowan A,Pignatelli M,Bodmer WF

更新日期：1997-09-16 00:00:00

abstract：:Exposure to childhood trauma (CT) increases the risk for psychosis and affects the development of brain structures, possibly through oxidative stress. As oxidative stress is also linked to psychosis, it may interact with CT, leading to a more severe clinical phenotype. In 133 patients with early psychosis (EPP), we ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alameda L,Fournier M,Khadimallah I,Griffa A,Cleusix M,Jenni R,Ferrari C,Klauser P,Baumann PS,Cuenod M,Hagmann P,Conus P,Do KQ

更新日期：2018-12-04 00:00:00

abstract：:Human T-lymphotropic virus type 1 (HTLV-1) is a retrovirus that causes malignant and inflammatory diseases in ∼10% of infected people. A typical host has between 10(4) and 10(5) clones of HTLV-1-infected T lymphocytes, each clone distinguished by the genomic integration site of the single-copy HTLV-1 provirus. The HTL...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Satou Y,Miyazato P,Ishihara K,Yaguchi H,Melamed A,Miura M,Fukuda A,Nosaka K,Watanabe T,Rowan AG,Nakao M,Bangham CR

更新日期：2016-03-15 00:00:00

abstract：:The T helper type 2 (Th2) cytokine genes Il4, Il5, and Il13 are contained within a 140-kb region of mouse chromosome 11 and their expression is controlled by a locus control region (LCR) embedded within this locus. The LCR is composed of a number of DNase I-hypersensitive sites (HSs), which are believed to encompass t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams A,Lee GR,Spilianakis CG,Hwang SS,Eisenbarth SC,Flavell RA

更新日期：2013-04-23 00:00:00

abstract：:Reports of structural differences between the brains of men and women, heterosexual and homosexual men, and male-to-female transsexuals and other men have been offered as evidence that the behavioral differences between these groups are likely caused by differences in the early development of the brain. However, a pos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cooke BM,Tabibnia G,Breedlove SM

更新日期：1999-06-22 00:00:00

abstract：:Adjacent paraffin sections of rat hypothalami fixed in Bouin's fluid were treated either with buffer or with luteinizing hormone-releasing hormone (LHRH) before immunocytochemical staining with anti-LHRH. Upon buffer pretreatment, pituitary gonadotrophs were unstained and hypothalamic fibers were stained. Upon LHRH pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sternberger LA,Greenwald JL,Hock D,Elger K,Forssmann WG

更新日期：1981-08-01 00:00:00

abstract：:Melanoma metastases can be categorized by gene expression for the presence of a T-cell-inflamed tumor microenvironment, which correlates with clinical efficacy of immunotherapies. T cells frequently recognize mutational antigens corresponding to nonsynonymous somatic mutations (NSSMs), and in some cases shared differe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spranger S,Luke JJ,Bao R,Zha Y,Hernandez KM,Li Y,Gajewski AP,Andrade J,Gajewski TF

更新日期：2016-11-29 00:00:00

abstract：:Social bees harbor a simple and specialized microbiota that is spatially organized into different gut compartments. Recent results on the potential involvement of bee gut communities in pathogen protection and nutritional function have drawn attention to the impact of the microbiota on bee health. However, the contrib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng H,Powell JE,Steele MI,Dietrich C,Moran NA

更新日期：2017-05-02 00:00:00

abstract：:Fibronectin (FN) forms the primitive fibrillar matrix in both embryos and healing wounds. To study the matrix in living cell cultures, we have constructed a cell line that secretes FN molecules chimeric with green fluorescent protein. These FN-green fluorescent protein molecules were assembled into a typical matrix th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohashi T,Kiehart DP,Erickson HP

更新日期：1999-03-02 00:00:00

abstract：:A power-law distribution of the length of perfectly conserved sequence from mouse/human whole-genome intersection and alignment is exhibited. Spatial correlations of these elements within the mouse genome are studied. It is argued that these power-law distributions and correlations are comprised in part by functional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salerno W,Havlak P,Miller J

更新日期：2006-08-29 00:00:00

abstract：:Studies on the structure-function relationship of diphtheria toxin are reported. New methods are described for the preparation of pure intact ("unnicked") toxin and for the preparation of the individual A and B chains. A biological assay method for the B chain is also presented, as well as a method for the labelling o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Everse J,Lappi DA,Beglau JM,Lee CL,Kaplan NO

更新日期：1977-02-01 00:00:00

abstract：:The lac operon of Escherichia coli spans approximately 5300 base pairs and includes the lacZ, lacY, and lacA genes in addition to the operator, promoter, and transcription termination regions. We report here the sequence of the lacA gene and the region distal to it, confirming the sequence of thiogalactoside transacet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hediger MA,Johnson DF,Nierlich DP,Zabin I

更新日期：1985-10-01 00:00:00

abstract：:Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product intercha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kitts P,Symington L,Burke M,Reed R,Sherratt D

更新日期：1982-01-01 00:00:00

abstract：:Rat brain and adrenal gland were analyzed by hybridization histochemistry using an RNA probe complementary to mRNA for tyrosine 3-hydroxylase (TyrOHase; tyrosine 3-monooxygenase, EC 1.14.16.2), by immunohistochemistry using TyrOHase antiserum, and by retrograde tracing using the fluorescent compound Fast blue. Cell bo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schalling M,Hökfelt T,Wallace B,Goldstein M,Filer D,Yamin C,Schlesinger DH

更新日期：1986-08-01 00:00:00

abstract：:The beta plasmid from Streptococcus faecalis strain DS5, which codes for resistance to erythromycin and lincomycin, was introduced into a Lancefield group F streptococcus, strain DR1501, by transformation. This strain, DR1501 (beta), was found to be an excellent donor of the beta plasmid and readily transferred the re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： LeBlanc DJ,Hawley RJ,Lee LN,St Martin EJ

更新日期：1978-07-01 00:00:00