当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Beta-catenin mutations in cell lines established from human colorectal cancers.

Beta-catenin mutations in cell lines established from human colorectal cancers.

Abstract：

:beta-catenin has functions as both an adhesion and a signaling molecule. Disruption of these functions through mutations of the beta-catenin gene (CTNNB1) may be important in the development of colorectal tumors. We examined the entire coding sequence of beta-catenin by reverse transcriptase-PCR (RT-PCR) and direct sequencing of 23 human colorectal cancer cell lines from 21 patients. In two cell lines, there was apparent instability of the beta-catenin mRNA. Five different mutations (26%) were found in the remaining 21cell lines (from 19 patients). A three-base deletion (codon 45) was identified in the cell line HCT 116, whereas cell lines SW 48, HCA 46, CACO 2, and Colo 201 each contained single-base missense mutations (codons 33, 183, 245, and 287, respectively). All 23 cell lines had full-length beta-catenin protein that was detectable by Western blotting and that coprecipitated with E-cadherin. In three of the cell lines with CTNNB1 mutations, complexes of beta-catenin with alpha-catenin and APC were detectable. In SW48 and HCA 46, however, we did not detect complexes of beta-catenin protein with alpha-catenin and APC, respectively. These results show that selection of CTNNB1 mutations occurs in up to 26% of colorectal cancers from which cell lines are derived. In these cases, mutation selection is probably for altered beta-catenin function, which may significantly alter intracellular signaling and intercellular adhesion and may serve as a complement to APC mutations in the early stages of tumorigenesis.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Ilyas M,Tomlinson IP,Rowan A,Pignatelli M,Bodmer WF

doi

10.1073/pnas.94.19.10330

subject

Has Abstract

pub_date

1997-09-16 00:00:00

pages

10330-4

issue

19

eissn

0027-8424

issn

1091-6490

journal_volume

94

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • The identification of an osteoclastogenesis inhibitor through the inhibition of glyoxalase I.

    abstract：:Osteoclasts, bone-resorptive multinucleated cells derived from hematopoietic stem cells, are associated with many bone-related diseases, such as osteoporosis. Osteoclast-targeting small-molecule inhibitors are valuable tools for studying osteoclast biology and for developing antiresorptive agents. Here, we have discov...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0712239105

    authors： Kawatani M,Okumura H,Honda K,Kanoh N,Muroi M,Dohmae N,Takami M,Kitagawa M,Futamura Y,Imoto M,Osada H

    更新日期：2008-08-19 00:00:00

  • Identification of gene expression profile of dorsal root ganglion in the rat peripheral axotomy model of neuropathic pain.

    abstract：:Phenotypic modification of dorsal root ganglion (DRG) neurons represents an important mechanism underlying neuropathic pain. However, the nerve injury-induced molecular changes are not fully identified. To determine the molecular alterations in a broader way, we have carried out cDNA array on the genes mainly made fro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.122231899

    authors： Xiao HS,Huang QH,Zhang FX,Bao L,Lu YJ,Guo C,Yang L,Huang WJ,Fu G,Xu SH,Cheng XP,Yan Q,Zhu ZD,Zhang X,Chen Z,Han ZG,Zhang X

    更新日期：2002-06-11 00:00:00

  • A circuit for navigation in Caenorhabditis elegans.

    abstract：:Caenorhabditis elegans explores its environment by interrupting its forward movement with occasional turns and reversals. Turns and reversals occur at stable frequencies but irregular intervals, producing probabilistic exploratory behaviors. Here we dissect the roles of individual sensory neurons, interneurons, and mo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0409009101

    authors： Gray JM,Hill JJ,Bargmann CI

    更新日期：2005-03-01 00:00:00

  • Exploiting the synthetic lethality between terminal respiratory oxidases to kill Mycobacterium tuberculosis and clear host infection.

    abstract：:The recent discovery of small molecules targeting the cytochrome bc 1 :aa 3 in Mycobacterium tuberculosis triggered interest in the terminal respiratory oxidases for antituberculosis drug development. The mycobacterial cytochrome...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1706139114

    authors： Kalia NP,Hasenoehrl EJ,Ab Rahman NB,Koh VH,Ang MLT,Sajorda DR,Hards K,Grüber G,Alonso S,Cook GM,Berney M,Pethe K

    更新日期：2017-07-11 00:00:00

  • Isolation and expression of rat liver sepiapterin reductase cDNA.

    abstract：:Sepiapterin reductase (7,8-dihydrobiopterin: NADP+ oxidoreductase, EC 1.1.1.153) catalyzes the terminal step in the biosynthetic pathway for tetrahydrobiopterin, the cofactor necessary for aromatic amino acid hydroxylation. We report here the isolation of a cDNA clone for rat liver sepiapterin reductase. The cDNA has ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.16.6436

    authors： Citron BA,Milstien S,Gutierrez JC,Levine RA,Yanak BL,Kaufman S

    更新日期：1990-08-01 00:00:00

  • Alloreactive immune responses of transgenic mice expressing a foreign transplantation antigen in a soluble form.

    abstract：:Transfection of cells with the H-2Kk gene lacking the transmembrane and cytoplasmic segments resulted in secretion of the H-2Kk protein, as determined by immunoprecipitation with monoclonal anti-H-2Kk antibodies. Transgenic (H-2b X H-2d)F1 mice were established carrying integrated copies of the modified H-2Kk gene. Ex...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.7.2269

    authors： Arnold B,Dill O,Küblbeck G,Jatsch L,Simon MM,Tucker J,Hämmerling GJ

    更新日期：1988-04-01 00:00:00

  • Single molecule physics and chemistry.

    abstract：:New experiments using scanning probe microcopies and advanced optical methods allow us to study molecules as individuals, not just as populations. The findings of these studies not only include the confirmation of results expected from studies of bulk matter, but also give substantially new information concerning the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.20.11075

    authors： Bai C,Wang C,Xie XS,Wolynes PG

    更新日期：1999-09-28 00:00:00

  • Isolation and characterization of delta-subspecies of protein kinase C from rat brain.

    abstract：:The delta-subspecies of protein kinase C (delta PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose fast flow, phenyl 5PW, heparin 5PW, hydroxyapatite, and Mono Q columns. The purified enzyme was a doublet with molecula...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.5.1592

    authors： Ogita K,Miyamoto S,Yamaguchi K,Koide H,Fujisawa N,Kikkawa U,Sahara S,Fukami Y,Nishizuka Y

    更新日期：1992-03-01 00:00:00

  • Poly(A) binding protein abundance regulates eukaryotic translation initiation factor 4F assembly in human cytomegalovirus-infected cells.

    abstract：:By commandeering cellular translation initiation factors, or destroying those dispensable for viral mRNA translation, viruses often suppress host protein synthesis. In contrast, cellular protein synthesis proceeds in human cytomegalovirus (HCMV)-infected cells, forcing viral and cellular mRNAs to compete for limiting ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1202829109

    authors： McKinney C,Perez C,Mohr I

    更新日期：2012-04-10 00:00:00

  • RAPT1, a mammalian homolog of yeast Tor, interacts with the FKBP12/rapamycin complex.

    abstract：:Rapamycin is a potent immunosuppressant that blocks the G1/S transition in antigen-activated T cells and in yeast. The similar effects of rapamycin in animal cells and yeast suggest that the biochemical steps affected by rapamycin are conserved. Using a two-hybrid system we isolated mammalian clones that interact with...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.26.12574

    authors： Chiu MI,Katz H,Berlin V

    更新日期：1994-12-20 00:00:00

  • Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain.

    abstract：:A recent major conceptual advance has been the recognition of the importance of immune system-neuronal interactions in the modulation of brain function, one example of which is spinal pain processing in neuropathic states. Here, we report that in peripheral nerve-injured rats, the lysosomal cysteine protease cathepsin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0610811104

    authors： Clark AK,Yip PK,Grist J,Gentry C,Staniland AA,Marchand F,Dehvari M,Wotherspoon G,Winter J,Ullah J,Bevan S,Malcangio M

    更新日期：2007-06-19 00:00:00

  • Goniometer-based femtosecond crystallography with X-ray free electron lasers.

    abstract：:The emerging method of femtosecond crystallography (FX) may extend the diffraction resolution accessible from small radiation-sensitive crystals and provides a means to determine catalytically accurate structures of acutely radiation-sensitive metalloenzymes. Automated goniometer-based instrumentation developed for us...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418733111

    authors： Cohen AE,Soltis SM,González A,Aguila L,Alonso-Mori R,Barnes CO,Baxter EL,Brehmer W,Brewster AS,Brunger AT,Calero G,Chang JF,Chollet M,Ehrensberger P,Eriksson TL,Feng Y,Hattne J,Hedman B,Hollenbeck M,Holton JM,Keab

    更新日期：2014-12-02 00:00:00

  • Franck-Condon picture of incoherent neutron scattering.

    abstract：:A spectroscopic interpretation of incoherent neutron scattering experiments is presented which is based on Franck-Condon-type probabilities for scattering-induced transitions between quantum states of the target. The resulting expressions for the scattering functions enable an energy landscape-oriented analysis of neu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1718720115

    authors： Kneller GR

    更新日期：2018-09-18 00:00:00

  • Haploinsufficiency of telomerase reverse transcriptase leads to anticipation in autosomal dominant dyskeratosis congenita.

    abstract：:Dyskeratosis congenita is a rare inherited disorder characterized by abnormal skin manifestations. Morbidity and mortality from this disease is usually due to bone marrow failure, but idiopathic pulmonary fibrosis and an increased cancer predisposition also occur. Families with autosomal dominant dyskeratosis congenit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0508124102

    authors： Armanios M,Chen JL,Chang YP,Brodsky RA,Hawkins A,Griffin CA,Eshleman JR,Cohen AR,Chakravarti A,Hamosh A,Greider CW

    更新日期：2005-11-01 00:00:00

  • Macrophage activation: dissociation of cytotoxic activity from Ia-A antigen expression.

    abstract：:Peritoneal macrophages were obtained from DBA/2 mice that were untreated or after the injection of bacillus Calmette-Guerin (BCG), thioglycollate broth, proteose-peptone broth, or gamma-irradiated P-815 tumor cells. These macrophages were "activated" to become cytotoxic for a fibroblast cell line (L 929) by the additi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.7.2031

    authors： Blumenthal EJ,Roberts WK,Vasil A,Talmage DW

    更新日期：1983-04-01 00:00:00

  • Kinetics of mature T-cell development in the thymus.

    abstract：:We have reexamined the balance between cell birth, cell maturation, and cell death in the thymus by labeling dividing thymocytes and their progeny in vivo with [3H]-thymidine, isolating clearly defined subpopulations by fluorescence-activated cell sorting, and determining the distribution of label by autoradiography. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.7.2579

    authors： Egerton M,Scollay R,Shortman K

    更新日期：1990-04-01 00:00:00

  • Class I MHC molecules on hematopoietic cells can support intrathymic positive selection of T cell receptor transgenic T cells.

    abstract：:The identity of cells that mediate positive selection of CD8(+) T cells was investigated in two T cell receptor (TCR) transgenic systems. Irradiated beta(2)-microglobulin mutant mice or mice with mutations in both the K(b) and D(b) genes were repopulated with fetal liver cells from class I(+) TCR transgenic mice. In t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.20.11470

    authors： Zerrahn J,Volkmann A,Coles MC,Held W,Lemonnier FA,Raulet DH

    更新日期：1999-09-28 00:00:00

  • Meiotic recombination frequencies are affected by nutritional states in Saccharomycescerevisiae.

    abstract：:Meiotic recombination in the yeast Saccharomyces cerevisiae is initiated by programmed double-strand breaks at selected sites throughout the genome (hotspots). alpha-Hotspots are binding sites for transcription factors. Double-strand breaks at alpha-hotspots require binding of transcription factor but not high levels ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.201529598

    authors： Abdullah MF,Borts RH

    更新日期：2001-12-04 00:00:00

  • Rosiglitazone increases dendritic spine density and rescues spine loss caused by apolipoprotein E4 in primary cortical neurons.

    abstract：:Convergent evidence has revealed an association between insulin resistance and Alzheimer's disease (AD), and the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist, rosiglitazone, an insulin sensitizer and mitochondrial activator, improves cognition in patients with early or mild-to-moderate AD. Apo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0709906104

    authors： Brodbeck J,Balestra ME,Saunders AM,Roses AD,Mahley RW,Huang Y

    更新日期：2008-01-29 00:00:00

  • Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.

    abstract：:Many bacteria use acyl homoserine lactone signals to monitor cell density in a type of gene regulation termed quorum sensing and response. Synthesis of these signals is directed by homologs of the luxi gene of Vibrio fischeri. This communication resolves two critical issues concerning the synthesis of the V. fischeri ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.18.9505

    authors： Schaefer AL,Val DL,Hanzelka BL,Cronan JE Jr,Greenberg EP

    更新日期：1996-09-03 00:00:00

  • Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules.

    abstract：:Hybridization of human T cells with an azaguanine-resistant human T cell line gave rise to T hybrid cell lines secreting several immunoregulatory molecules. Analyses of karyotypes, HLA phenotypes, and other surface phenotypes, such as T-cell-specific antigens or receptors for sheep erythrocytes and the patterns of mit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.12.7717

    authors： Okada M,Yoshimura N,Kaieda T,Yamamura Y,Kishimoto T

    更新日期：1981-12-01 00:00:00

  • Design of a mechanical clutch-based needle-insertion device.

    abstract：:Insertion of trocars, needles, and catheters into unintended tissues or tissue compartments results in hundreds of thousands of complications annually. Current methods for blood vessel cannulation or epidural, chest tube, and initial trocar placement often involve the blind pass of a needle through several layers of t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0808274106

    authors： Bassett EK,Slocum AH,Masiakos PT,Pryor HI 2nd,Farokhzad OC,Karp JM

    更新日期：2009-04-07 00:00:00

  • Listener evaluations of new and Old Italian violins.

    abstract：:Old Italian violins are routinely credited with playing qualities supposedly unobtainable in new instruments. These qualities include the ability to project their sound more effectively in a concert hall-despite seeming relatively quiet under the ear of the player-compared with new violins. Although researchers have l...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1619443114

    authors： Fritz C,Curtin J,Poitevineau J,Tao FC

    更新日期：2017-05-23 00:00:00

  • Ohnologs are overrepresented in pathogenic copy number mutations.

    abstract：:A number of rare copy number variants (CNVs), including both deletions and duplications, have been associated with developmental disorders, including schizophrenia, autism, intellectual disability, and epilepsy. Pathogenicity may derive from dosage sensitivity of one or more genes contained within the CNV locus. To un...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1309324111

    authors： McLysaght A,Makino T,Grayton HM,Tropeano M,Mitchell KJ,Vassos E,Collier DA

    更新日期：2014-01-07 00:00:00

  • Overexpression of LMO4 induces mammary hyperplasia, promotes cell invasion, and is a predictor of poor outcome in breast cancer.

    abstract：:The zinc finger protein LMO4 is overexpressed in a high proportion of breast carcinomas. Here, we report that overexpression of a mouse mammary tumor virus (MMTV)-Lmo4 transgene in the mouse mammary gland elicits hyperplasia and mammary intraepithelial neoplasia or adenosquamous carcinoma in two transgenic strains wit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0502990102

    authors： Sum EY,Segara D,Duscio B,Bath ML,Field AS,Sutherland RL,Lindeman GJ,Visvader JE

    更新日期：2005-05-24 00:00:00

  • Epithelial membrane protein-1 is a biomarker of gefitinib resistance.

    abstract：:We describe a molecular resistance biomarker to gefitinib, epithelial membrane protein-1 (EMP-1). Gefitinib is a small-molecule inhibitor that competes for the ATP-binding site on EGF receptor (EGFR) and has been approved for patients with advanced lung cancers. Treatment with gefitinib has resulted in clinical benefi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0502113102

    authors： Jain A,Tindell CA,Laux I,Hunter JB,Curran J,Galkin A,Afar DE,Aronson N,Shak S,Natale RB,Agus DB

    更新日期：2005-08-16 00:00:00

  • Interactions of metal-nucleotide complexes with aspartate carbamoyltransferase in the crystalline state.

    abstract：:We report the results of crystallographic difference maps at 3.0-A resolution of complexes of metal-nucleoside triphosphates with aspartate carbamoyltransferase (carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2) from Escherichia coli. The complexes Gd3+-ATP, Al3+-ATP, and Gd3+-CTP bind to the allosteric...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.23.7171

    authors： Honzatko RB,Lipscomb WN

    更新日期：1982-12-01 00:00:00

  • Application of a polarizable force field to calculations of relative protein-ligand binding affinities.

    abstract：:An explicitly polarizable force field based exclusively on quantum data is applied to calculations of relative binding affinities of ligands to proteins. Five ligands, differing by replacement of an atom or functional group, in complexes with three serine proteases-trypsin, thrombin, and urokinase-type plasminogen act...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0803847105

    authors： Khoruzhii O,Donchev AG,Galkin N,Illarionov A,Olevanov M,Ozrin V,Queen C,Tarasov V

    更新日期：2008-07-29 00:00:00

  • Functional interaction with filamin A and intracellular Ca2+ enhance the surface membrane expression of a small-conductance Ca2+-activated K+ (SK2) channel.

    abstract：:For an excitable cell to function properly, a precise number of ion channel proteins need to be trafficked to distinct locations on the cell surface membrane, through a network and anchoring activity of cytoskeletal proteins. Not surprisingly, mutations in anchoring proteins have profound effects on membrane excitabil...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1323541111

    authors： Rafizadeh S,Zhang Z,Woltz RL,Kim HJ,Myers RE,Lu L,Tuteja D,Singapuri A,Bigdeli AA,Harchache SB,Knowlton AA,Yarov-Yarovoy V,Yamoah EN,Chiamvimonvat N

    更新日期：2014-07-08 00:00:00

  • Development and persistence of kindling epilepsy are impaired in mice lacking glial cell line-derived neurotrophic factor family receptor alpha 2.

    abstract：:Seizure activity regulates gene expression for glial cell line-derived neurotrophic factor (GDNF) and neurturin (NRTN), and their receptor components, the transmembrane c-Ret tyrosine kinase and the glycosylphosphatidylinositol-anchored GDNF family receptor (GFR) alpha 1 and alpha 2 in limbic structures. We demonstrat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.22.12312

    authors： Nanobashvili A,Airaksinen MS,Kokaia M,Rossi J,Asztély F,Olofsdotter K,Mohapel P,Saarma M,Lindvall O,Kokaia Z

    更新日期：2000-10-24 00:00:00