Abstract:
:Although epicardial blood flow can be restored by an early intervention in most cases, a lack of adequate reperfusion at the microvascular level is often a limiting prognostic factor of acute myocardial infarction (AMI). Our group has recently found that paracrine factors secreted from apoptotic peripheral blood mononuclear cells (APOSEC) attenuate the extent of myocardial injury. The aim of this study was to determine the influence of APOSEC on microvascular obstruction (MVO) in a porcine AMI model. A single dose of APOSEC was intravenously injected in a closed chest reperfused infarction model. MVO was determined by magnetic resonance imaging and cardiac catheterization. Role of platelet function and vasodilation were monitored by means of ELISA, flow cytometry, aggregometry, western blot and myographic experiments in vitro and in vivo. Treatment of AMI with APOSEC resulted in a significant reduction of MVO. Platelet activation markers were reduced in plasma samples obtained during AMI, suggesting an anti-aggregatory capacity of APOSEC. This finding was confirmed by in vitro tests showing that activation and aggregation of both porcine and human platelets were significantly impaired by co-incubation with APOSEC, paralleled by vasodilator-stimulated phosphoprotein (VASP)-mediated inhibition of platelets. In addition, APOSEC evidenced a significant vasodilatory capacity on coronary arteries via p-eNOS and iNOS activation. Our data give first evidence that APOSEC reduces the extent of MVO during AMI, and suggest that modulation of platelet activation and vasodilation in the initial phase after myocardial infarction contributes to the improved long-term outcome in APOSEC treated animals.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Hoetzenecker K,Assinger A,Lichtenauer M,Mildner M,Schweiger T,Starlinger P,Jakab A,Berényi E,Pavo N,Zimmermann M,Gabriel C,Plass C,Gyöngyösi M,Volf I,Ankersmit HJdoi
10.1007/s00395-012-0292-2subject
Has Abstractpub_date
2012-09-01 00:00:00pages
292issue
5eissn
0300-8428issn
1435-1803journal_volume
107pub_type
杂志文章abstract::Urapidil (URA) is used to treat acute hypertension in patients with coronary artery disease, but the effect of URA on the performance of ischemic myocardium has not yet been investigated. The present study was intended to assess the function of ischemic myocardium following URA administration. In eight anesthetized (p...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907115
更新日期:1990-05-01 00:00:00
abstract::We describe diastolic properties of the myocardium in terms of stress-strain relations. In a mathematical analysis, the equation sigma = alpha(e beta.epsilon-1) of the stress-strain curve can be changed by an increase in the exponent beta or the multiplicative constant alpha. It can be experimentally shown that in hyp...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11289-2_26
更新日期:1987-01-01 00:00:00
abstract::Total global ischaemia of the normothermic working rat heart caused an initial positive inotropic response characterized by vigorous contractions. After +/- 15 s this response reached a peak whereafter the isotonic contraction amplitude started to decline. After +/- 3.5 min the heart ceased to beat. The low level of h...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906862
更新日期:1987-05-01 00:00:00
abstract:OBJECTIVE:Chemokines control the migration of leukocytes to inflamed tissue, and in particular monocyte chemoattractant protein (MCP)-1 has been implicated in the pathogenesis of several cardiovascular disorders such as chronic heart failure (CHF) and myocarditis. We hypothesised that MCP-1 may directly contribute to a...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s003950170042
更新日期:2001-07-01 00:00:00
abstract::Receptor binding studies usually show heterogenic characteristics of the binding isotherm. Scatchard transformation for graphical linearization of the data presentation affects the data inhomogeneously with respect to the experimental error. Thus a non-linear fitting procedure should be preferred for data analysis. Fu...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-642-72376-6_2
更新日期:1984-01-01 00:00:00
abstract::Protection from postconditioning has been documented in in situ animal models and it has been proposed that it is targeting circulating leukocytes. We therefore tested whether postconditioning can protect leukocyte-free, buffer-perfused rabbit hearts. Infarct size was measured with triphenyltetrazolium staining. In co...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-004-0498-4
更新日期:2005-01-01 00:00:00
abstract::The success of therapies targeting myocardial reperfusion injury is limited, while the cardioprotective impact of mitigating ischemia-related damage remains less explored. We have recently shown in a pig model that the intravenous administration of a modified atorvastatin preparation during ischemia attenuates the ris...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-019-0760-z
更新日期:2019-11-28 00:00:00
abstract::This study was aimed to assess the relationship among the voltage-dependent processes underlying the excitation-contraction coupling, viz. force restitution (FR), transmembrane Ca fluxes and Ca release. The experiments (n = 22) were performed on voltage-clamped dog trabeculae in which force and slow inward current wer...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00795054
更新日期:1992-09-01 00:00:00
abstract::Recent publications shown mitochondrial localization of the enzyme nitric oxide synthase (NOS) in a number of tissues. However, conflicting results about mitochondrial NOS (mtNOS) immunoreactivity and enzymatic activity are available to date in the literature. In this study we purified mitochondria from rat hearts and...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-003-0454-3
更新日期:2004-05-01 00:00:00
abstract::Exercise training is a well-known non-pharmacological strategy for the prevention and treatment of cardiovascular diseases. Despite the established phenotypic knowledge, the molecular signature of exercise-induced cardiac remodeling remains poorly characterized. The great majority of studies dedicated to this topic us...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-014-0454-5
更新日期:2015-01-01 00:00:00
abstract::Calcium transport functions of mitochondria and sarcoplasmic reticulum (SR) were studied without prior extraction using isolated rat heart myocytes permeabilized with saponin. Calcium uptake by SR was rapid and its affinity was high in comparison to calcium uptake by mitochondria, which had a higher capacity. The sens...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907975
更新日期:1989-05-01 00:00:00
abstract::Denervation deprives the heart of its normal adrenergic and cholinergic control via the sympathetic and parasympathetic pathways. In a heart which is blood supplied by a donor animal of the same species, normal contractility is maintained, probably by blood borne catecholamines or possibly by unknown inotropic agents ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907778
更新日期:1976-01-01 00:00:00
abstract::The relationship between ischemic contracture and no-reflow phenomenon was studied in 59 isolated rat hearts during global ischemia. The contracture was measured by a water-filled balloon catheter placed in the left ventricular lumen. The time of onset of contracture was changed by preischemic infusion with buffer con...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02005340
更新日期:1982-07-01 00:00:00
abstract::It is generally believed that the increased contractility and tachycardia of the hyperthyroid heart are a result of thyroid hormone-induced alterations of the mechanical and electrical properties of the heart, respectively. We compared the contractility (dP/dtmax) and the spontaneous beating rate of hyperthyroid and e...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906958
更新日期:1988-11-01 00:00:00
abstract::During myocardial ischemia, both the myocardial and serum TNFalpha concentrations are rapidly increased within the area at risk. With prolongation of ischemia and development of cardiomyocyte necrosis, the TNFalpha concentration increases also in the surrounding viable portions of the myocardium. Indeed, in the scenar...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-003-0431-x
更新日期:2004-01-01 00:00:00
abstract::1. Studies on three models of cardiac hypertrophy (aortic constriction, application of isoproterenol, daily administrations of 3,3'5-triiodo-L-thyronine, respectively) reveal that the enhancement of de novo synthesis of adenine nucleotides occurs very early during the development of cardiac hypertorphy and always prec...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906368
更新日期:1977-03-01 00:00:00
abstract::Cardiac mass, cell size and capillary supply were studied in the hearts of spontaneously hypertensive rats (SHR) and compared to genetically similar non-hypertensive Wistar-Kyoto (WKY) of three adult ages: 5, 15 and 23 months. The left ventricular weight of SHR was not significantly greater than that of WKY at 5 month...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908138
更新日期:1984-07-01 00:00:00
abstract::Atrophy of the rat heart induced by hemodynamic unloading after heterotopic transplantation is associated with impaired relaxation while systolic function remains normal when compared to the heart of the recipient animal. To identify possible underlying mechanisms for the above, we studied some aspects of membrane cal...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788540
更新日期:1995-11-01 00:00:00
abstract::Ca2+ overload followed by after-contractions was induced by perfusion of mammalian papillary muscles with O-K0+, high Ca2+ solution. The effect of lipotrophic agents (dexamethasone, indomethacin) or free radical scavengers (alpha-tocopherol, reduced glutathione, synthetic antioxidant) was tested at different stages of...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908177
更新日期:1985-07-01 00:00:00
abstract:AIMS:There is a need for better identification of patients at high risk for malignant arrhythmias who would benefit from implantable cardioverter defibrillators (ICD). The purpose of this study was to assess whether the C825T polymorphism in the G-protein beta3 subunit gene, GNB3, might serve as a genetic marker for re...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-006-0600-9
更新日期:2006-09-01 00:00:00
abstract::Vasoconstrictory and vasodilatory hormone systems may be important in the regulation of peripheral vascular resistance and renal hemodynamics in the early phase of heart failure. The activity of the renin-angiotensin-aldosterone system (RAAS), the sympathetic nervous activity, and, as possible counterregulating system...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907058
更新日期:1987-03-01 00:00:00
abstract::Atrial fibrosis is important for the pathogenesis of atrial fibrillation (AF) but the underlying signal transduction is incompletely understood. We therefore studied the role of microRNA-21 (miR-21) and its downstream target Sprouty 1 (Spry1) during atrial fibrillation. Left atria (LA) from patients with AF showed a 2...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-012-0278-0
更新日期:2012-09-01 00:00:00
abstract::Cardiac alpha- and beta-adrenoceptors and the positive inotropic effects of several adenylate cyclase dependent and independent agents have been measured in papillary muscle strips from patients without, as well as with moderate and severe heart failure. The number of beta-adrenoceptors was found to be decreased depen...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02650352
更新日期:1989-01-01 00:00:00
abstract::Hearts preconditioned by brief ischemia are characterized by a reduced rate of cellular purine metabolite production during subsequent prolonged ischemia; the purpose of this study was to determine if transient exogenous adenosine pretreatment can mimic this phenomenon. The accumulation of interstitial fluid (ISF) pur...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00796210
更新日期:1997-12-01 00:00:00
abstract::Increased activity of the sympathetic system is an important feature contributing to the pathogenesis and progression of chronic heart failure. While the mechanisms and consequences of enhanced norepinephrine release from sympathetic nerves have been intensely studied, the role of the adrenal gland in the development ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-011-0166-z
更新日期:2011-06-01 00:00:00
abstract::Ceramide has been shown to be a key signaling molecule involved in the apoptotic effect of tumor necrosis factor alpha (TNF-alpha) and other cytokines. Given the importance of cytokines such as TNF-alpha in myocardial ischemia-reperfusion injury, we hypothesize that ceramide is increased during ischemia or reperfusion...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s003950170057
更新日期:2001-05-01 00:00:00
abstract::The proximal segments of the left coronary artery are foreshortended in the usual projections. By additional cranio-caudal (half axial) projections the X-ray beam can be directed perpendicular to these segments. In some patients lesions especially of the left main coronary artery or the proximal LAD can be seen or acc...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907787
更新日期:1976-01-01 00:00:00
abstract::Recent studies have demonstrated the presence of autoantibodies against alpha(1)-adrenoceptor (alpha(1)-AAB) in the serum of patients with primary hypertension, and that these autoantibodies exert adrenergic-agonist-like effects. However, their role in the development of hypertension remains unclear. The current study...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-009-0020-8
更新日期:2009-09-01 00:00:00
abstract::The clinical use of positive inotropic agents has been associated with increased mortality, with proarrhythmia speculated to be a contributing factor. This study compares the arrhythmogenic potentials of six positive inotropic agents representing different mechanistic classes: the beta-adrenergic agonist dobutamine, t...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s003950050181
更新日期:2000-06-01 00:00:00
abstract::Cardiac disease is associated with deleterious emission of mitochondrial reactive oxygen species (mito-ROS), as well as enhanced oxidation and activity of the sarcoplasmic reticulum (SR) Ca2+ release channel, the ryanodine receptor (RyR2). The transfer of Ca2+ from the SR via RyR2 to mitochondria is thought to play a ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-020-0797-z
更新日期:2020-05-22 00:00:00