Abstract:
OBJECTIVE:Chemokines control the migration of leukocytes to inflamed tissue, and in particular monocyte chemoattractant protein (MCP)-1 has been implicated in the pathogenesis of several cardiovascular disorders such as chronic heart failure (CHF) and myocarditis. We hypothesised that MCP-1 may directly contribute to an inflammatory response in the cardiomyocytes, and in the present study we examined in adult rat cardiomyocytes: (i) the effect of tumour necrosis factor (TNF)alpha on MCP-1 production, (ii) the effect of MCP-1 on production of other inflammatory cytokines, and (iii) if the anti-inflammatory cytokine interleukin (IL)-10 could suppress any TNFalpha-induced MCP- 1 production. METHODS:We used enzyme immunoassays, RNase protection assays and slot blot analysis to measure protein and mRNA levels of various cytokines in adult rat cardiomyocyte cultures. RESULTS:(i) We found a approximately 6.4-fold increase of the MCP-1 level accompanied by an increase in MCP-1 mRNA accumulation in cardiomyocyte cultures after TNFalpha stimulation. (ii) In contrast, TNFalpha had no effect on IL-10 and only a modest effect on IL-1beta and IL-6 levels in these cells. (iii) Importantly, MCP-1 stimulated inflammatory response in cardiomyocytes by enhancing IL- 1beta and IL-6 levels in these cells as found at both the protein and mRNA level. (iv) Co-stim-ulation with IL-10 resulted in a approximately 55% reduction in TNFalpha-stimulated MCP-1 levels in cardiomyocyte culture supernatants. CONCLUSION:The present study demonstrates for the first time that MCP- 1 can directly affect cardiomyocytes, and we introduce MCP-1 as a potential enhancer and IL- 10 as a potential suppresser of inflammatory responses within the myocardium.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Damås JK,Aukrust P,Ueland T,Odegaard A,Eiken HG,Gullestad L,Sejersted OM,Christensen Gdoi
10.1007/s003950170042keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
345-52issue
4eissn
0300-8428issn
1435-1803journal_volume
96pub_type
杂志文章abstract::We chronically implanted hygroscopic occluders around the left circumflex coronary artery in 49 anesthetized young male domestic pigs and we studied the development of a collateral circulation at 4, 8, 12, and 26 weeks after implantation. At these time intervals groups of animals were again anesthetized, the hearts we...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908204
更新日期:1989-09-01 00:00:00
abstract::Long QT syndrome is a potentially life-threatening disease characterized by delayed repolarization of cardiomyocytes, QT interval prolongation in the electrocardiogram, and a high risk for sudden cardiac death caused by ventricular arrhythmia. The genetic type 3 of this syndrome (LQT3) is caused by gain-of-function mu...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-016-0530-0
更新日期:2016-03-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-012-0306-0
更新日期:2012-11-01 00:00:00
abstract::Exercise training is a well-known non-pharmacological strategy for the prevention and treatment of cardiovascular diseases. Despite the established phenotypic knowledge, the molecular signature of exercise-induced cardiac remodeling remains poorly characterized. The great majority of studies dedicated to this topic us...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-014-0454-5
更新日期:2015-01-01 00:00:00
abstract::The aim of this study was to clarify the role of altered diabetic vascular reactivity in ischaemic heart disease. In diabetic condition, the necrotic area of myocardial infarction was significantly extended and myocardial oedema failed to develop after administration of norepinephrine 2 or 48 hours after ligation of t...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907942
更新日期:1982-09-01 00:00:00
abstract::Our understanding of what constitutes "normal" collagen organization in wound healing after myocardial injury and how such organization is achieved is still in its infancy. However, once this knowledge has been obtained, it may suggest methods of enhancing or even optimizing healing, and thus provide us with the possi...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s003950050201
更新日期:1998-01-01 00:00:00
abstract::Verapamil, at a dose of 1 mg/kg, was given intravenously to anesthetized cats one hour after coronary artery occlusion. Verapamil significantly reduced mean arterial blood pressure, but produced an increase in heart rate, partially offsetting the reduction in myocardial oxygen demand resulting from the reduction in pr...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907648
更新日期:1979-09-01 00:00:00
abstract::It is shown that hypertrophied rat myocardium exhibits marked prolongation of its action potential during non-essential changes both in the resting potential, as well as in the amplitude of the action potential, and during a moderate but nonsignificant reduction in the maximum upstroke velocity. When examining action ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02001397
更新日期:1980-01-01 00:00:00
abstract::Myocardial ischemia-reperfusion (I/R) injury leads to intensive sympathetic nervous system (SNS) activation and inflammatory reactions. Whether renal sympathetic denervation (RDN) could be a new therapeutic strategy to modulate I/R inflammation and reduce infarct size after myocardial I/R injury needs to be explored. ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-020-0776-4
更新日期:2020-01-13 00:00:00
abstract::We have examined the sodium-calcium exchange stoichiometry in Langendorff-perfused rabbit hearts using gamma-emitting tracers under conditions of sodium pump inhibition. Following a 60-min perfusion with 10(-5) acetylstrophanthidin, and extracellular concentrations [Na]o = 70 mM and [Ca]o = 300 microM, intracellular s...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907365
更新日期:1988-05-01 00:00:00
abstract::Implicit in attempts to characterize and purify biologically active factors is the premise that the bioassay system employed will show a progressive increase in the response as the concentration of the responsible factor increases. We employed the hamster cheek pouch to assay the neovascularization potential of growth...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907406
更新日期:1986-05-01 00:00:00
abstract::A survey was made of vagal afferents whose endings originated in cardiopulmonary areas of the Rhesus monkey. Recordings of action potentials from single fiber preparations of the left cervical vagus were made in both open and closed chest monkeys. A total of 425 receptors were identified in sixteen animals. These cons...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02023598
更新日期:1977-07-01 00:00:00
abstract::An increase in extracellular potassium concentration from 4 to 16 mmol/l caused a decrease in membrane potential from -92 to -59 mV and selectively diminished the earlier of two contraction components of guinea-pig papillary muscles at 0.2 Hz stimulation frequency in the presence of noradrenaline. The influence on the...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907089
更新日期:1987-09-01 00:00:00
abstract::Sarcoplasmic reticulum (SR) from left ventricles of rats that developed spontaneous hypertension was studied in vitro. Similar increases of left ventricular mass were found when grouping the animals into mild and severe hypertensives (average systolic arterial pressure of 168 +/- 4 and 202 +/- 6 mmHg, respectively). T...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907362
更新日期:1988-05-01 00:00:00
abstract::In this paper, we review the hypothesis that activated tissue renin-angiotensin systems play a detrimental role in heart failure. The main arguments for this idea are discussed: a) tissue renin-angiotensin systems behave functionally distinct from the circulating renin-angiotensin system; b) tissue renin-angiotensin s...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/BF00795368
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:Ischemic or hypoxic preconditioning has been shown, in multicellular preparations, to reduce post-ischemic injury. In the present study, we attempted to develop a model of preconditioning in isolated rat myocytes in order to facilitate investigation into the mechanism of preconditioning. METHODS:The protecti...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788907
更新日期:1996-05-01 00:00:00
abstract::Endothelin-1 elicited a concentration-dependent positive inotropic effect on the isolated rabbit, guinea pig, and rat, but not on the dog ventricular myocardium. The duration of isometric contractions was prolonged by endothelin-1 in a concentration-dependent manner, mainly by prolongation of relaxation time. Phorbol ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::A reproducible tourniquet-shock has been produced in hind limbs of dogs by unilateral and bilateral extremity ischemia. The following parameters have been measured for analysing the function of the cardiovascular system: mean aortic pressure, heart rate, cardiac output, intraventricular pressure and left ventricular p...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01927866
更新日期:1976-03-01 00:00:00
abstract::Transient receptor potential melastatin-7 (TRPM7) channels have been recently reported in human atrial fibroblasts and are believed to mediate fibrogenesis in human atrial fibrillation. The present study investigates whether TRPM7 channels are expressed in human atrial myocytes using whole-cell patch voltage-clamp, RT...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-012-0282-4
更新日期:2012-09-01 00:00:00
abstract::A new experimental in-vitro model is described, which was used for studying prostacyclin (PGI2)-thromboxane A2 (TXA2)-interactions. Langendorff hearts of guinea pigs are perfused at constant volume with Krebs-Henseleit buffer and washed human platelets (4 x 10(8)/min). PGI2 and TXA2 release are measured by bioassay. T...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908344
更新日期:1981-09-01 00:00:00
abstract::Ischemic heart disease is the main cause of death worldwide and is accelerated by increased levels of low-density lipoprotein cholesterol (LDL-C). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a potent circulating regulator of LDL-C through its ability to induce degradation of the LDL receptor (LDLR) in the...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-017-0619-0
更新日期:2017-05-01 00:00:00
abstract::There is a growing need for pharmacological agents to manage cardiovascular disease in the rapidly growing elderly population. Here, we determine if acetaminophen is efficacious in decreasing age-related increases in cardiac reactive oxygen species (ROS) and apoptosis in aging Fischer 344 X Brown Norway rats. Compared...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-010-0094-3
更新日期:2010-07-01 00:00:00
abstract::32 patients were studied by His-bundle recordings to examine the extent to which frequency and autonomic nervous system influence the conduction velocity in the subdivisions (atrium, AV-node, His-Purkinje system) of the normal PR-interval. The measurements were performed during sinus rhythm and three electrically indu...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract:OBJECTIVE:Two types of hemodynamic overload, pressure and volume overload, result in morphologically distinct types of cardiac remodeling. We explored the possibility that distinct hemodynamic overload may differentially activate the signal transduction pathway. METHODS:Pressure and volume overload were induced by tho...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-004-0482-7
更新日期:2004-09-01 00:00:00
abstract::N,N-dimethylsphingosine (DMS) has been documented to be in vitro protective against myocardial ischemia-reperfusion injury (IRI) and can recruit CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs), which may participate in the cardioprotection. We hypothesized that when in vivo applied after a myocardial ischemia, DMS ma...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-016-0548-3
更新日期:2016-05-01 00:00:00
abstract::X-ray microanalysis was used to compare chemically untreated cryosections of quick-frozen myocardial tissue in "caffeine contracture" with cryosections of normal muscle. Our goal was to find out if it is possible by means of this method to detect changes in the calcium compartmentalization of the myocardial cell occur...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02001396
更新日期:1980-01-01 00:00:00
abstract::Both the severity and duration of postischemic myocardial dysfunction ("stunned" myocardium) are unpredictable and may vary considerably between subjects that underwent apparently similar ischemic insults. To explain this heterogeneous response of the heart to ischemia and reperfusion, we investigated the determinants...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00799686
更新日期:1996-03-01 00:00:00
abstract::Cardiomyopathy is one of the most common causes of chronic heart failure worldwide. Mutations in the gene encoding nexilin (NEXN) occur in patients with both hypertrophic and dilated cardiomyopathy (DCM); however, little is known about the pathophysiological mechanisms and relevance of NEXN to these disorders. Here, w...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-015-0522-5
更新日期:2016-01-01 00:00:00
abstract::This paper reviews the recent data concerning the force-frequency relations in experimental animals with heart failure. After a brief overview of the nature of these relations, which are induced by 2 opposite phenomena, the potentiation of force of contraction by heart rate and the restitution process, the paper revie...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s003950050212
更新日期:1998-01-01 00:00:00
abstract:OBJECTIVES:The impact of acute collagen disruption by the disulfide donor, 5,5'-dithio-2-nitrobenzoic acid (DTNB) on ventricular properties was tested in rat hearts. METHODS:Collagen was degraded acutely in 13 isolated, isovolumically contracting rat hearts by perfusion with 1 mM DTNB added to Krebs-Henseleit solution...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788632
更新日期:1997-06-01 00:00:00