Abstract：
:Transient receptor potential melastatin-7 (TRPM7) channels have been recently reported in human atrial fibroblasts and are believed to mediate fibrogenesis in human atrial fibrillation. The present study investigates whether TRPM7 channels are expressed in human atrial myocytes using whole-cell patch voltage-clamp, RT-PCR and Western blotting analysis. It was found that a gradually activated TRPM7-like current was recorded with a K(+)- and Mg(2+)-free pipette solution in human atrial myocytes. The current was enhanced by removing extracellular Ca(2+) and Mg(2+), and the current increase could be inhibited by Ni(2+) or Ba(2+). The TRPM7-like current was potentiated by acidic pH and inhibited by La(3+) and 2-aminoethoxydiphenyl borate. In addition, Ca(2+)-activated TRPM4-like current was recorded in human atrial myocytes with the addition of the Ca(2+) ionophore A23187 in bath solution. RT-PCR and Western immunoblot analysis revealed that in addition to TRPM4, TRPM7 channel current, mRNA and protein expression were evident in human atrial myocytes. Interestingly, TRPM7 channel protein, but not TRPM4 channel protein, was significantly increased in human atrial specimens from the patients with atrial fibrillation. Our results demonstrate for the first time that functional TRPM7 channels are present in human atrial myocytes, and the channel expression is upregulated in the atria with atrial fibrillation.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Zhang YH,Sun HY,Chen KH,Du XL,Liu B,Cheng LC,Li X,Jin MW,Li GRdoi
10.1007/s00395-012-0282-4subject
Has Abstractpub_date
2012-09-01 00:00:00pages
282issue
5eissn
0300-8428issn
1435-1803journal_volume
107pub_type
杂志文章abstract：:Cardiomyopathy is one of the most common causes of chronic heart failure worldwide. Mutations in the gene encoding nexilin (NEXN) occur in patients with both hypertrophic and dilated cardiomyopathy (DCM); however, little is known about the pathophysiological mechanisms and relevance of NEXN to these disorders. Here, w...
journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950170013
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
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doi：10.1007/BF01907976
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-11374-5_15
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/BF00795358
更新日期：1996-01-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 社论
doi：10.1007/BF00788525
更新日期：1993-01-01 00:00:00
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
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doi：10.1007/BF00788735
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/BF02070160
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
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更新日期：1993-01-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-006-0589-0
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02005337
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906800
更新日期：1978-11-01 00:00:00
abstract：:The influence of PGI2 and iloprost on the contents of high energy phosphates, cAMP, glycolytic metabolites and on cardiac performance was investigated in open-chest dogs subjected to 3 h of ischaemia, induced by occlusion of the left anterior descending coronary artery. The administration of drugs for 2 h, starting 1 ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907227
更新日期：1987-11-01 00:00:00
