Abstract：
:N,N-dimethylsphingosine (DMS) has been documented to be in vitro protective against myocardial ischemia-reperfusion injury (IRI) and can recruit CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs), which may participate in the cardioprotection. We hypothesized that when in vivo applied after a myocardial ischemia, DMS may be cardioprotective by recruiting Tregs. Myocardial IRI was induced in C57BL/6 mice by occluding the left main coronary arteries followed by relaxation, and DMS (0.43 mg/kg) was intravenously injected 5 min after the onset of ischemia. We found that in wild-type (WT) mice, compared with the ischemia-reperfusion group, DMS reduced the infarct size (47.1 ± 8.9 vs. 33.1 ± 3.4 %, p < 0.01), and neutrophil infiltration at 24 h reperfusion (R) evaluated by TTC and immunohistochemical staining, respectively, and increased the aggregation of Tregs [(6 ± 1)/mm(2) vs. (30 ± 4)/mm(2), p < 0.01], peaking at 1 h R by immunofluorescence staining, with reduced gene expression of inflammatory factors at 4 h R in the reperfused myocardium by real-time PCR. This protection was abolished by phosphatidylinositol 3-kinase (PI3K)/Akt inhibitor or Tregs-depleting antibody. Relative to WT mice, the cardioprotection conferred by T cell- and B cell- deficient Rag2 knockout (KO) mice was not strengthened by DMS or by DMS and the adoptive transfer of Tregs from WT mice, but was abolished by DMS and WT non-Tregs and was recaptured by the cotransfer with WT Tregs but not with Akt1(+/-) mice-derived Tregs. In conclusion, applied at an early stage of ischemia, DMS may be in vivo protective against myocardial IRI by recruiting Tregs via PI3K/Akt pathway.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Fang J,Hu F,Ke D,Yan Y,Liao Z,Yuan X,Wu L,Jiang Q,Chen Ldoi
10.1007/s00395-016-0548-3subject
Has Abstractpub_date
2016-05-01 00:00:00pages
32issue
3eissn
0300-8428issn
1435-1803pii
10.1007/s00395-016-0548-3journal_volume
111pub_type
杂志文章abstract：:Recent attention has focused on the concept that mitochondrial dynamics-that is, the balance between mitochondrial fusion and fission (fragmentation)-may play a pivotal role in determining cell fate in the setting of myocardial ischemia-reperfusion injury. In this regard, there is an emerging consensus that: (1) ische...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-016-0578-x
更新日期：2016-09-01 00:00:00
abstract：:The hormone-sensitive adenylate cyclase is a multi-component system embedded in the lipid bilayer of the plasma membrane and serves as a signal transduction system for various membrane receptors. The complete system consists of various receptor molecules, which sensitize the external ligands, the effector enzyme adeny...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907422
更新日期：1986-01-01 00:00:00
abstract：:Short repeated cycles of peripheral ischemia/reperfusion (I/R) can protect distant organs from subsequent prolonged I/R injury; a phenomenon known as remote ischemic preconditioning (RIPC). A RIPC-mediated release of humoral factors might play a key role in this protection and vascular endothelial cells are potential ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-015-0474-9
更新日期：2015-03-01 00:00:00
abstract：:Low-flow perfusion of paced (5 Hz) rat heart Langendorff preparation with Tyrode solution for 60 min caused a reduction or disappearance of left ventricular pressure amplitude. After 60 min of low-flow perfusion myocardial concentrations of high energy phosphates, as well as the energy charged potential, were diminish...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907015
更新日期：1990-01-01 00:00:00
abstract：:Therapeutic angiogenesis improves tissue ischemia by supplementing and supporting the intrinsic process of angiogenesis. Besides direct administration of angiogenic growth factor proteins, injection of naked DNA, non-viral vectors and viral vector constructs carrying angiogenic genes have been used. A novel approach i...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-004-0447-x
更新日期：2004-03-01 00:00:00
abstract：:Recent studies demonstrated that NADPH oxidase 2 (NOX2) expression in myocardium after ischemia-reperfusion (IR) is significantly upregulated. However, the underlying mechanisms remain unknown. This study aims to determine if nuclear cardiac myosin light chain 2 (MYL2), a well-known regulatory subunit of myosin, funct...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-015-0494-5
更新日期：2015-01-01 00:00:00
abstract：:To evaluate the hypothesis that maintenance of the integrity of myocardial membrane systems and prevention of Ca2+ influx into the cell are significant in the survival of ischaemic tissue, the effect of trifluoperazine and lysolecithin, were tested on the recovery of globally ischaemic rat hearts. Trifluoperazine incr...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01908180
更新日期：1985-07-01 00:00:00
abstract：:Phospholamban is a regulatory phosphoprotein which modulates the active transport of Ca2+ by the cardiac sarcoplasmic reticular Ca(2+)-ATPase enzyme (SERCA2) into the lumen of the sarcoplasmic reticulum. Phospholamban, which is a reversible inhibitor of SERCA2, represses the enzyme's activity, and this inhibition is r...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00794064
更新日期：1997-01-01 00:00:00
abstract：:Cardiac physiology and hypertrophy are regulated by the phosphorylation status of many proteins, which is partly controlled by a poorly defined type 2A protein phosphatase-alpha4 intracellular signalling axis. Quantitative PCR analysis revealed that mRNA levels of the type 2A catalytic subunits were differentially exp...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-017-0625-2
更新日期：2017-07-01 00:00:00
abstract：:Ceramide has been shown to be a key signaling molecule involved in the apoptotic effect of tumor necrosis factor alpha (TNF-alpha) and other cytokines. Given the importance of cytokines such as TNF-alpha in myocardial ischemia-reperfusion injury, we hypothesize that ceramide is increased during ischemia or reperfusion...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950170057
更新日期：2001-05-01 00:00:00
abstract：:Necrosis is a type of cell death characterized by a gain in cell volume, swelling of organelles, rupture of the plasma membrane and subsequent loss of intracellular contents. For a long time, the process has been considered as a merely accidental and uncontrolled form of cell death, but accumulating evidence suggests ...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-011-0192-x
更新日期：2011-09-01 00:00:00
abstract：:The genetic defect in the cardiomyopathic (CM) hamster is a mutation in the glycoprotein-sarcoglycan (a component of the dystrophin-glycoprotein complex). Apoptosis has been identified in skeletal muscle of dystrophin-deficient mice, and therefore the role of myocardial apoptosis in relation to oncosis in causing myoc...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s395-002-8389-4
更新日期：2002-01-01 00:00:00
abstract：:A methodological approach based on Frank's pressure-volume (P-V) diagram and model calculations for analyzing the determinants of stroke volume (SV) under conditions of altered ventricular geometrical configuration is discussed with respect to the underlying geometrical models. The approach is generalized in the sense...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02190551
更新日期：1991-03-01 00:00:00
abstract：:Our understanding of what constitutes "normal" collagen organization in wound healing after myocardial injury and how such organization is achieved is still in its infancy. However, once this knowledge has been obtained, it may suggest methods of enhancing or even optimizing healing, and thus provide us with the possi...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s003950050201
更新日期：1998-01-01 00:00:00
abstract：:The effects of a c-myc antisense phosphorothioate DNA oligonucleotide were assessed on the proliferation rate of human arterial smooth muscle cells (HSMCs). Compared to a control oligonucleotide the antisense oligonucleotide suppressed the proliferation of HSMCs in a concentration-dependent manner without a major cyto...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788668
更新日期：1992-11-01 00:00:00
abstract：:We tested the hypotheses that myocardial infarction in mice would lead to progressively worsening heart failure 12-18 weeks later and that exercise testing would provide a suitable means to evaluate left ventricular function sequentially. C57BL/6 mice (n = 69) underwent left coronary artery ligation (n = 50) or thorac...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950200013
更新日期：2002-05-01 00:00:00
abstract：:A reproducible tourniquet-shock has been produced in hind limbs of dogs by unilateral and bilateral extremity ischemia. The following parameters have been measured for analysing the function of the cardiovascular system: mean aortic pressure, heart rate, cardiac output, intraventricular pressure and left ventricular p...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01927866
更新日期：1976-03-01 00:00:00
abstract：:The aim of this study was to investigate the mechanism of beta-adrenergic preconditioning (BPC). The roles of adenosine and its receptor subtypes, the generation of oxygen free radicals (ROS) and activation of the K(ATP) channels as well as the phosphoinositide-3-kinase (PI(3)K)/PKB/Akt and extracellular signal-regula...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0281-5
更新日期：2012-09-01 00:00:00
abstract：:Dopexamine is a newly developed sympathetic catecholamine which combines dopaminergic (DA-1) and beta 2-adrenergic agonist activity with only minor beta 1-adrenergic action. Thereby, this compound exerts systemic and preferential renal vasodilation, causing afterload reduction, increases in cardiac output, and improve...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/BF02650357
更新日期：1989-01-01 00:00:00
abstract：:The relationship between ischemic contracture and no-reflow phenomenon was studied in 59 isolated rat hearts during global ischemia. The contracture was measured by a water-filled balloon catheter placed in the left ventricular lumen. The time of onset of contracture was changed by preischemic infusion with buffer con...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02005340
更新日期：1982-07-01 00:00:00
abstract：:The characterization of various ion transport systems has led to a better understanding of the effects, which seem to take part in the impairment of ischemic and reperfused cardiac tissue. This review discusses the role of the Na+/H+ exchange system in the pathophysiology of ischemia and reperfusion and the beneficial...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/BF00795411
更新日期：1993-09-01 00:00:00
abstract：:The quantitative predominance of free and total cholesterol over the amount of mural calcium is a most significant criterion of healthy human coronary arteries during the whole life span (0-90 years). However, this normal ratio increasingly changes as soon as arteriosclerotic alterations of the coronary walls set in. ...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-85660-0_15
更新日期：1994-01-01 00:00:00
abstract：:The stroke volume at rest of 81 test subjects with no evident cardiac or circulatory dysfunction was determined by impedance cardiography and the measurements were repeated after 11 weeks. In order to attain comparable and reproducible conditions, 44 test subjects were chosen who showed heart rates at rest not higher ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906800
更新日期：1978-11-01 00:00:00
abstract：:PDE3 cyclic nucleotide phosphodiesterase inhibitors raise cAMP and cGMP content in cardiac and vascular myocytes. Their administration to patients with dilated cardiomyopathy leads to improvements in hemodynamic parameters in the short term but reduces survival with chronic administration. The reasons for this 'biphas...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s003950200035
更新日期：2002-01-01 00:00:00
abstract：:The present study was designed to test the hypothesis that in hypertrophied myocardium of patients with hypertrophic obstructive cardiomyopathy (HOCM) a reduced contractile reserve provided by frequency dependent potentiation of force of contraction contributes to the myocardial dysfunction. Myectomy was performed in ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950050134
更新日期：1999-04-01 00:00:00
abstract：:Ex vivo multicellular preparations are essential tools to study tissue physiology. Among them, the recent methodological and technological developments in living myocardial slices (LMS) are attracting increasing interest by the cardiac research field. Despite this, this research model remains poorly perceived and util...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-020-00822-y
更新日期：2020-09-10 00:00:00
abstract：:During long-term treatment with amiodarone, slowing of conduction through the atrioventricular node, a prolongation of the QT-interval, and a prolongation of the atrial and ventricular myocardial refractoriness always developed. During short-term treatment, these effects were not found, except for depression of the AV...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02190546
更新日期：1991-03-01 00:00:00
abstract：:Although it has long been recognized that thyroid hormone is an effective positive inotrope, its efficacy in supporting hemodynamics in the acute setting of ischaemia and reperfusion (R) without worsening reperfusion injury remains largely unknown. Thus, we investigated the effects of triiodothyronine (T3) on reperfus...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-008-0758-4
更新日期：2009-01-01 00:00:00
abstract：:We investigated whether preconditioning could protect several cultured cell lines, to determine whether the protection is specific for cells derived from different myogenic and non-myogenic sources. Ischemia was simulated by centrifugation of cells into a pellet, and cell viability was determined by hypotonic trypan b...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788726
更新日期：1996-11-01 00:00:00
abstract：:An adult rat heart myocyte preparation was used to study the uptake and metabolism of the 1-14C-labelled fatty acids decanoate, laurate, myristate, and palmitate. Metabolism was saturable, but free fatty acid accumulation was not. Use of metabolic inhibitors indicated that metabolism and not uptake was rate limiting a...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
