Abstract:
OBJECTIVE:Ischemic or hypoxic preconditioning has been shown, in multicellular preparations, to reduce post-ischemic injury. In the present study, we attempted to develop a model of preconditioning in isolated rat myocytes in order to facilitate investigation into the mechanism of preconditioning. METHODS:The protective effect of a short period (10 min) of anoxia and reoxygenation against a subsequent longer period of anoxia was studied in single, electrically stimulated (0.2 Hz, 37 degrees C) adult rat cardiac myocytes. The control group received only the long period of anoxia. Three protocols were tested: Protocol 1 in which octanoate was the only substrate; Protocol 2 in which only glucose was present during all normoxic phases and during the preconditioning anoxia and octanoate alone during the prolonged period of anoxia and; Protocol 3 in which protocol 2 was repeated with the addition of adenosine (100 microM) and insulin (15 microU/ml) during the prolonged anoxic period. The end-point of assessment was loss of cell morphology, i.e., hypercontracture (death) or relengthening (survival) on reoxygenation following the prolonged anoxic period. Membrane integrity was also examined at the end of each protocol by observing if the cells excluded trypan blue. RESULTS:No protective effect of preconditioning on cell survival was observed in protocols 1 or 2. In contrast, in protocol 3, a significant protection was observed in the preconditioned versus control group (58% vs 27% survival respectively; p < 0.001). However, in the absence of preconditioning, adenosine and insulin provided no additional protection in the control group. No significant differences in trypan blue exclusion were observed between the groups in any protocol. CONCLUSIONS:These results suggest that preconditioning cannot protect against a subsequent period of anoxia where the accumulation of metabolic products, e.g., adenosine is prevented. However, that protection can be re-instated by the presence of adenosine and insulin during the period of prolonged anoxia. Furthermore, this study suggests that the preconditioning by anoxia may induce a change in the A1-receptor or its second messenger system such that adenosine is able to provide protection.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Cave AC,Adrian S,Apstein CS,Silverman HSdoi
10.1007/BF00788907subject
Has Abstractpub_date
1996-05-01 00:00:00pages
210-8issue
3eissn
0300-8428issn
1435-1803journal_volume
91pub_type
杂志文章abstract::The effects of the potassium channel openers (KCO), cromakalim or pinacidil, were evaluated in an anesthetized porcine model of pacing- and ischemia-induced ventricular fibrillation (VF). Hearts were paced at 180 bpm and the left anterior descending coronary artery was occluded until VF was induced. Reproducible times...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788735
更新日期:1994-03-01 00:00:00
abstract::Myocytes isolated from guinea pig ventricles were voltage-clamped using patch pipettes in the whole-cell configuration. For proper voltage control fast Na+ current was blocked by TTX or inactivated by an appropriate prepulse. Zero-load cell shortening was monitored by a photoelectric device. The mechanical response to...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907923
更新日期:1989-03-01 00:00:00
abstract::In the control perfused working rat hearts subjected to 25 min global ischemia, reperfusion resulted in a 50% recovery of the hemodynamic functions. A concentration-dependent improvement of this recovery and a reduction of the postischemic lactate dehydrogenase (LDH) release was caused by calmidazolium (CMZ), trifluop...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906948
更新日期:1989-11-01 00:00:00
abstract::Hypertension and resulting left ventricular hypertrophy was induced in young male Wistar rats (60 to 70 days old) by narrowing of one renal artery (Goldblatt II). 8 and 24 weeks after operation, myocardial oxygen consumption was measured on a modified in situ heart-lung preparation with nearly isovolumetric left ventr...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908041
更新日期:1982-05-01 00:00:00
abstract::Mitochondria in largely postmitotic cells (e.g. cardiomyocytes, neurons or skeletal muscle myotubes) have a limited life span of a few weeks. Their replacement during normal turnover requires an intergenomic coordination between the mitochondrial genome (mtDNA, encoding for 13 protein subunits of the respiratory chain...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-003-0421-z
更新日期:2003-07-01 00:00:00
abstract::We describe diastolic properties of the myocardium in terms of stress-strain relations. In a mathematical analysis, the equation sigma = alpha(e beta.epsilon-1) of the stress-strain curve can be changed by an increase in the exponent beta or the multiplicative constant alpha. It can be experimentally shown that in hyp...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11289-2_26
更新日期:1987-01-01 00:00:00
abstract::Nitroglycerin (GTN) and other organic nitrates are widely used vasodilators. Their side effects are development of nitrate tolerance and endothelial dysfunction. Given the potential of GTN to induce nitro-oxidative stress, we investigated the interaction between nitro-oxidative DNA damage and vascular dysfunction in e...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-016-0571-4
更新日期:2016-07-01 00:00:00
abstract::The effects of regional hypoxemia and ischemia on epicardial electrogram were studied in anesthetized, open-chest dogs. The left circumflex artery (LCx) was cannulated and perfused with either arterial blood or hypoxic solution. A contact electrode for recording monophasic action potential (MAP) was applied to the epi...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788537
更新日期:1995-11-01 00:00:00
abstract::In Langendorff-perfused guinea pig hearts, local myocardial perfusion was evaluated by analysis of FITC-dextran-150 elution kinetics after bolus injection. Locally estimated half-times of the monoexponential elution curves showed good correlation with global undisturbed coronary flow. After ligation of the left anteri...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01935807
更新日期:1984-01-01 00:00:00
abstract::N,N-dimethylsphingosine (DMS) has been documented to be in vitro protective against myocardial ischemia-reperfusion injury (IRI) and can recruit CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs), which may participate in the cardioprotection. We hypothesized that when in vivo applied after a myocardial ischemia, DMS ma...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-016-0548-3
更新日期:2016-05-01 00:00:00
abstract::Apart from local heterogeneities of myocardial blood flow, the regulation of vascular tone is equally complex. Changes in vascular tone are essential for the adaptation of coronary blood flow to varying metabolic demands. The majority of coronary vascular resistance is found in the microcirculation, that is, in vessel...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s003950050114
更新日期:1998-12-01 00:00:00
abstract::It was previously reported that inhibition of carnitine synthesis by 3-(2,2,2-trimethyl-hydrazinium) propionate (MET-88) restores left ventricular (LV) systolic and diastolic function in rats with myocardial infarction (MI). Preservation of the calcium uptake function of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) is ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s003950070032
更新日期:2000-10-01 00:00:00
abstract::This study was designed to evaluate whether the decreased early survival rate of diabetic rats submitted to acute experimental myocardial infarction can be improved by pretreatment with lidocaine. Male Wistar rats (+/- 210 g) were rendered diabetic with i.v. injection of streptozotocin (50 mg/kg), and only those prese...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907132
更新日期:1990-07-01 00:00:00
abstract::Exercise is an efficient strategy for myocardial protection against ischemia-reperfusion (IR) injury. Although endothelial nitric oxide synthase (eNOS) is phosphorylated and activated during exercise, its role in exercise-induced cardioprotection remains unknown. This study investigated whether modulation of eNOS acti...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-013-0389-2
更新日期:2013-11-01 00:00:00
abstract::The influence of changes in pre- and afterload, heart frequency and inotropic state on the difference between right ventricular dP/dtmax measured isovolumically and auxotonically has been investigated in canine heart-lung preparations. Isovolumic systoles were obtained by occluding the pulmonary artery during diastole...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01909767
更新日期:1976-09-01 00:00:00
abstract::In the isometrically contracting rat trabecular muscle strip preparation, contracture tension was induced either by 10 min of hypoxia (low contracture tension) or by 30 min of joint oxygen and glucose withdrawal (severe contracture tension) at optimum length. Using stepwise 0.1 mm releases, length-tension relationship...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908059
更新日期:1981-11-01 00:00:00
abstract::Two-weekly echocardiographic examinations were conducted in nine SHHF-Mc-fa(cp) rats in comparison with eight age-matched Wistar rats. In the SHHF-rats, characterized by progressive LV-dilation and decreasing contractile function between 77-87 weeks of age, left ventricular (LV) hypertrophy was most sensitively demons...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-003-0401-3
更新日期:2003-09-01 00:00:00
abstract::Successive ischemic episodes are commonly seen in clinical and experimental cardiology. Although prolonged abnormalities of the canine myocardium have been described following a single ischemic episode, it is not known whether myocardial injury is cumulative following multiple ischemic episodes. Dogs were subjected to...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908148
更新日期:1984-07-01 00:00:00
abstract::An adult rat heart myocyte preparation was used to study the uptake and metabolism of the 1-14C-labelled fatty acids decanoate, laurate, myristate, and palmitate. Metabolism was saturable, but free fatty acid accumulation was not. Use of metabolic inhibitors indicated that metabolism and not uptake was rate limiting a...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Sympathetic neural remodeling, which involves the inflammatory response, plays an important role in ventricular arrhythmias (VAs) after myocardial infarction (MI). Adrenergic receptors on macrophages potentially modulate the inflammatory response. We hypothesized that the increased level of catecholamines activates ma...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-020-0813-3
更新日期:2020-08-07 00:00:00
abstract::Vasoconstrictory and vasodilatory hormone systems may be important in the regulation of peripheral vascular resistance and renal hemodynamics in the early phase of heart failure. The activity of the renin-angiotensin-aldosterone system (RAAS), the sympathetic nervous activity, and, as possible counterregulating system...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907058
更新日期:1987-03-01 00:00:00
abstract::There is a growing need for pharmacological agents to manage cardiovascular disease in the rapidly growing elderly population. Here, we determine if acetaminophen is efficacious in decreasing age-related increases in cardiac reactive oxygen species (ROS) and apoptosis in aging Fischer 344 X Brown Norway rats. Compared...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-010-0094-3
更新日期:2010-07-01 00:00:00
abstract::32 patients were studied by His-bundle recordings to examine the extent to which frequency and autonomic nervous system influence the conduction velocity in the subdivisions (atrium, AV-node, His-Purkinje system) of the normal PR-interval. The measurements were performed during sinus rhythm and three electrically indu...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::The ability of the rhesus monkey to form coronary collaterals was tested in ten animals. Ameroid constrictors were implanted on the left circumflex coronary artery and allowed to remain for 12 weeks. One animal died of an acute myocardial infarction nine days after surgery; the remaining animals survived without clini...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906462
更新日期:1983-09-01 00:00:00
abstract::Studies were carried out in 39 barbiturate-anesthetized dogs to determine whether the renin-angiotensin system is important in control of hemodynamics and coronary flow during myocardial ischemia. Plasma renin activity (PRA) was 2.2 +/- 0.4 ng x ml-l x hr-1 immediately before coronary artery occlusion (CAO) and increa...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906463
更新日期:1983-09-01 00:00:00
abstract::The behavior of Ca(2+)-activated K+ channels of large conductance (BKCa) in smooth muscle cells, which were obtained from atherosclerotic plaque material (SMCP) and from media segments (SMCM) of human coronary arteries, were compared using the patch-clamp technique. Voltage-clamp protocols in cell-attached patches rev...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788518
更新日期:1997-08-01 00:00:00
abstract::The effect of 0.1 mg/kg Verapamil on the extent of the ischemic area following ligation of a branch of the left descending coronary artery was studied in anesthetized open chest dogs. The sum of the ST-segment elevation during the entire period of occlusion in the epicardial ECG was 15 mV in control infarction compare...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01905620
更新日期:1975-03-01 00:00:00
abstract::Ischaemic preconditioning is one of the most potent experimental modalities known to decrease infarct size after ischaemia and reperfusion. Much interest has been stimulated by the phenomenon of remote ischaemic conditioning (RIC), in which the preconditioning stimulus is applied to a limb remote from the heart to sti...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-013-0377-6
更新日期:2013-09-01 00:00:00
abstract::The renin angiotensin system and endothelium-derived vasoactive substances are both important regulators of vascular tone. Recent evidence suggests that the two systems may be tightly interconnected and drugs interfering with one system may also affect the other. Beside the circulating renin angiotensin system, a vasc...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/978-3-642-72497-8_2
更新日期:1993-01-01 00:00:00
abstract::From January 2020, coronavirus disease (COVID-19) originated in China has spread around the world. The disease is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The presence of myocarditis, cardiac arrest, and acute heart failure in COVID-19 patients suggests the existence of a relationshi...
journal_title:Basic research in cardiology
pub_type: 社论
doi:10.1007/s00395-020-0791-5
更新日期:2020-04-09 00:00:00