Abstract：
:It was previously reported that inhibition of carnitine synthesis by 3-(2,2,2-trimethyl-hydrazinium) propionate (MET-88) restores left ventricular (LV) systolic and diastolic function in rats with myocardial infarction (MI). Preservation of the calcium uptake function of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) is one of the possible mechanisms by which MET-88 alleviates hemodynamic dysfunction. To test this hypothesis, the effects of MET-88 on protein content of SERCA2 were evaluated using the same rat model of heart failure. Myocardial protein content of hexokinase, which is one of the key enzymes of glucose utilization, was also measured. Either MET-88 (MET-88 group) or a placebo (MI group) was administered for 20 days to rats with MI induced by coronary artery ligation. The control group underwent sham surgery (no ligation) and received placebo. In LV myocardial homogenates, the myocardial SERCA2 protein content was 32% lower (p<0.05) in the MI group than in the control group. However, in the MET-88 group myocardial SERCA2 content was the same as in the control group. Hexokinase I protein content was 29 % lower (p<0.05) in the MI group compared with the control. In contrast, hexokinase II protein content did not differ significantly among the three groups. Consequently, inhibition of carnitine synthesis ameliorates depression of SERCA2 and hexokinase I protein content which may reduce tissue damage caused by MI.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Yonekura K,Eto Y,Yokoyama I,Matsumoto A,Sugiura S,Momomura S,Kirimoto T,Hayashi Y,Omata M,Aoyagi Tdoi
10.1007/s003950070032keywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
343-8issue
5eissn
0300-8428issn
1435-1803journal_volume
95pub_type
杂志文章abstract：:In the isometrically contracting rat trabecular muscle strip preparation, contracture tension was induced either by 10 min of hypoxia (low contracture tension) or by 30 min of joint oxygen and glucose withdrawal (severe contracture tension) at optimum length. Using stepwise 0.1 mm releases, length-tension relationship...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01908059
更新日期：1981-11-01 00:00:00
abstract：:Pressure-overloaded cardiac hypertrophy was induced by abdominal aortic constriction in 10-week-old male Wistar rats. 24-26 weeks after aortic constriction, the hearts were excised and a myocardial mechanical study was performed using isolated left ventricular papillary muscles. There was no significant difference in ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907024
更新日期：1987-07-01 00:00:00
abstract：:A model of vascular endothelial cell is proposed to describe the mechanisms by which cytosolic calcium (Cai) is modulated and endothelium-derived relaxing factor (EDRF) and prostacyclin (PGI2) are released when the cell is stimulated by agonist. The intracellular Ca2+ store of the model cell is comprised of a superfic...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00796518
更新日期：1992-07-01 00:00:00
abstract：OBJECTIVE:Two types of hemodynamic overload, pressure and volume overload, result in morphologically distinct types of cardiac remodeling. We explored the possibility that distinct hemodynamic overload may differentially activate the signal transduction pathway. METHODS:Pressure and volume overload were induced by tho...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-004-0482-7
更新日期：2004-09-01 00:00:00
abstract：:The assessment of endothelium-mediated modulation of coronary vasomotor tone in the intact human circulation under physiologic conditions requires very precise determination of both epicardial artery diameters, reflecting effects within the conduit vessels, as well as coronary blood flow, reflecting effects within the...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-72461-9_18
更新日期：1991-01-01 00:00:00
abstract：:Endothelin-1 elicited a concentration-dependent positive inotropic effect on the isolated rabbit, guinea pig, and rat, but not on the dog ventricular myocardium. The duration of isometric contractions was prolonged by endothelin-1 in a concentration-dependent manner, mainly by prolongation of relaxation time. Phorbol ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:32 patients were studied by His-bundle recordings to examine the extent to which frequency and autonomic nervous system influence the conduction velocity in the subdivisions (atrium, AV-node, His-Purkinje system) of the normal PR-interval. The measurements were performed during sinus rhythm and three electrically indu...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Cardiac hypertrophy which occurs during chronic mechanical overload is one of the numerous examples of biological adaptation to environmental requirements. As such, it is obtained at random by trial and error, and adaptation represents the sum of various modifications in gene expression, including the shift in isoform...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-72474-9_15
更新日期：1992-01-01 00:00:00
abstract：:Experimental evidence indicates that ischemic glycolysis improves myocardial tolerance to low flow ischemia and anoxia, and cellular membrane disruption signals and/or causes transition to irreversible ischemic injury. The objective of this study was to determine the impact of ischemic glycolysis on membrane integrity...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950170013
更新日期：2001-11-01 00:00:00
abstract：:Rate or frequency-dependence is a characteristic property of antiarrhythmic drugs belonging to the Vaughan William classes I and III. The rate-dependence of class I drugs (i.e., increasing blockade of fast Na(+)-channels with faster rates) results from periodical drug binding to Na(+)-channel sites which are preferabl...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s003950050236
更新日期：1998-01-01 00:00:00
abstract：:The effects of regional hypoxemia and ischemia on epicardial electrogram were studied in anesthetized, open-chest dogs. The left circumflex artery (LCx) was cannulated and perfused with either arterial blood or hypoxic solution. A contact electrode for recording monophasic action potential (MAP) was applied to the epi...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788537
更新日期：1995-11-01 00:00:00
abstract：:The cardiac renin angiotensin system (RAS) is the target for number of therapeutic interventions which proved successful in heart failure. Angiotensin converting enzyme (ACE) inhibitors belong to the most efficient strategies available and angiotensin receptor (ATR) antagonists may be comparably effective. The direct ...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s003950050207
更新日期：1998-01-01 00:00:00
abstract：:Pentostatin (2-deoxycoformycin) is a potent inhibitor of adenosine deaminase and has been demonstrated to augment endogenous adenosine levels during regional and global myocardial ischemia. Based on the rationale that increasing endogenous adenosine during ischemia may be cardioprotective, the objective of this study ...
journal_title：Basic research in cardiology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF00789447
更新日期：1995-03-01 00:00:00
abstract：:Recent studies demonstrated that NADPH oxidase 2 (NOX2) expression in myocardium after ischemia-reperfusion (IR) is significantly upregulated. However, the underlying mechanisms remain unknown. This study aims to determine if nuclear cardiac myosin light chain 2 (MYL2), a well-known regulatory subunit of myosin, funct...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-015-0494-5
更新日期：2015-01-01 00:00:00
abstract：:The focal adhesion and microfilament-associated protein VASP is a major substrate of both cAMP- and cGMP-dependent protein kinase in intact human platelets. The recent elucidation of the primary VASP structure and identity of VASP binding proteins suggest that VASP is an important component of focal contacts which lin...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788712
更新日期：1996-09-01 00:00:00
abstract：:K201 has previously been shown to reduce diastolic contractions in vivo during β-adrenergic stimulation and elevated extracellular calcium concentration ([Ca(2+)](o)). The present study characterised the effect of K201 on electrically stimulated and spontaneous diastolic sarcoplasmic reticulum (SR)-mediated Ca(2+) rel...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-011-0218-4
更新日期：2011-11-01 00:00:00
abstract：:We studied TTX-sensitive Na channels in dissociated single ventricular cells from neonatal rats using the patch clamp method for single channel and whole cell recording. In both cases, slowly inactivating or window currents were observed that decayed in a biexponential fashion. Customary models of Na current kinetics ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Recent findings on the induction of anti-apoptotic gene expression in ischemic/reperfused hearts encouraged us to investigate whether ischemic/reperfused hearts may be protected against apoptosis induction. To analyze this hypothesis we performed studies on isolated perfused hearts of rat. For apoptosis induction, hea...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950200053
更新日期：2002-11-01 00:00:00
abstract：:Ultrasonic techniques for the measurement of ventricular dimensions are widely used in acute and chronic experiments. Implantation of ultrasonic crystals is associated with reversible and irreversible myocardial damage which might limit the interpretation of the obtained results, in particular during acute experiments...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907029
更新日期：1987-07-01 00:00:00
abstract：:It is shown that hypertrophied rat myocardium exhibits marked prolongation of its action potential during non-essential changes both in the resting potential, as well as in the amplitude of the action potential, and during a moderate but nonsignificant reduction in the maximum upstroke velocity. When examining action ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02001397
更新日期：1980-01-01 00:00:00
abstract：:Apart from local heterogeneities of myocardial blood flow, the regulation of vascular tone is equally complex. Changes in vascular tone are essential for the adaptation of coronary blood flow to varying metabolic demands. The majority of coronary vascular resistance is found in the microcirculation, that is, in vessel...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s003950050114
更新日期：1998-12-01 00:00:00
abstract：:Cardiac disease is associated with deleterious emission of mitochondrial reactive oxygen species (mito-ROS), as well as enhanced oxidation and activity of the sarcoplasmic reticulum (SR) Ca2+ release channel, the ryanodine receptor (RyR2). The transfer of Ca2+ from the SR via RyR2 to mitochondria is thought to play a ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-020-0797-z
更新日期：2020-05-22 00:00:00
abstract：:We hypothesized that nandrolone (ND)-abuse induces cardiac hypertrophy, increases myocardial susceptibility to ischemia/reperfusion (I/R) injury, and reduces responsiveness to postconditioning (PostC) cardioprotection. Wistar-rats were ND treated for 2 weeks (short_ND) or 10 weeks (long_ND). Vehicle-treated rats serve...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-010-0143-y
更新日期：2011-05-01 00:00:00
abstract：:The role of miR-92a on vascular remodelling after injury is currently unknown. Thus, the aim of the present study was to evaluate the role of miR-92a on rat endothelial and vascular smooth muscle cells proliferation and migration in vitro as well as after balloon injury or arterial stenting in vivo. MiR-92a was highly...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0296-y
更新日期：2012-09-01 00:00:00
abstract：:Coronary vasomotion of normal, stenotic, and poststenotic vessel segments was studied in 18 patients with coronary artery disease at rest, during submaximal bicycle exercise, and 5 min after sublingual nitroglycerin or oral isosorbide-dinitrate (ISDN) spray. Patients were divided into two groups: group 1 consisted of ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-11038-6_28
更新日期：1990-01-01 00:00:00
abstract：:Regional glucose utilization of the developing fetal feline heart was assessed during three stages of gestation and compared with the maternal heart and non-pregnant controls. The specific aims were to determine: 1) if glucose utilization by the whole heart changes from early to late gestation; 2) if there are differe...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02191527
更新日期：1991-07-01 00:00:00
abstract：:Ventricular and atrial cardiac muscle cells isolated from the adult rat have been cultured and characterized. Once established in culture these myocytes resemble morphologically their in vivo counterparts except that they are flattened on the surface of the culture flask. These cultured cells possess differentiated ul...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:We have examined the sodium-calcium exchange stoichiometry in Langendorff-perfused rabbit hearts using gamma-emitting tracers under conditions of sodium pump inhibition. Following a 60-min perfusion with 10(-5) acetylstrophanthidin, and extracellular concentrations [Na]o = 70 mM and [Ca]o = 300 microM, intracellular s...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907365
更新日期：1988-05-01 00:00:00
abstract：:The PKC hypothesis and its variants are attractive but unproved. The chief difficulty is the lack of an objective means to assess whether or not PKC has been activated in hearts preconditioned in vivo with ischemia or in hearts preconditioned by pharmacologic means. The strongest evidence is supporting the PKC hypothe...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00797206
更新日期：1997-01-01 00:00:00
abstract：:A canine model for a standardized induction of collaterals is presented with a fixed external constrictor that is not designed to induce an occlusion of the coronary artery and at least over the timespan of 6 weeks does not impair perfusion under resting conditions in the myocardium-at-risk. The coronary constriction ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906671
更新日期：1983-03-01 00:00:00
