当前位置： SCI文献检索 > BASIC RESEARCH IN CARDIOLOGY期刊下所有文献 > Activation of distinct signal transduction pathways in hypertrophied hearts by pressure and volume overload.

Activation of distinct signal transduction pathways in hypertrophied hearts by pressure and volume overload.

Abstract：

OBJECTIVE:Two types of hemodynamic overload, pressure and volume overload, result in morphologically distinct types of cardiac remodeling. We explored the possibility that distinct hemodynamic overload may differentially activate the signal transduction pathway. METHODS:Pressure and volume overload were induced by thoracic aortic banding and carotid-jugular shunt formation in rabbits, respectively. Phosphorylation activities of mitogen-activated protein (MAP) kinase families, Akt, and signal transducer and activator of transcription (STAT) 3 in the left ventricular myocardium were determined by Western blotting using phospho-specific antibodies and were compared between hypertrophied hearts by pressure and volume overload. RESULTS:Pressure and volume overload produced concentric and eccentric cardiac hypertrophy in rabbits, respectively. In pressure-overloaded hearts, extracellular signal-regulated kinase (ERK) 1/2, p38 MAP kinase, and STAT3 were transiently activated prior to hypertrophic changes. In contrast, activation of ERK1/2, but not p38 MAP kinase and STAT3, was observed only at 12 weeks after shunt surgery. Pressure overload evoked short and biphasic activation of Akt at 15 min and 1 day after aortic banding. In contrast, volume overload induced sustained activation of Akt from 1 day to 1 week. Concordant phosphorylation of downstream targets of Akt, glycogen synthase kinase-3beta (GSK-3beta) and p70 ribosomal S6 kinase (p70(S6K)), in response to Akt activation was observed at 15 min after pressure overload. However in volume-overloaded hearts, phosphorylation of GSK-3beta and p70(S6K) was observed at 6 weeks and at 6 and 12 weeks, respectively, and was not coincident with Akt activation. These findings suggest that phosphorylation of GSK-3beta and p70(S6K) is regulated by an alternative pathway other than Akt in volume-overloaded hearts. CONCLUSION:Pressure and volume overload-induced cardiac hypertrophy is associated with distinct patterns of activation of signal transduction pathways. These data may suggest that stimulus-specific heterogeneity in the signaling pathway plays a role in determining the type of cardiac hypertrophy.

journal_name

Basic Res Cardiol

journal_title

Basic research in cardiology

authors

Miyamoto T,Takeishi Y,Takahashi H,Shishido T,Arimoto T,Tomoike H,Kubota I

doi

10.1007/s00395-004-0482-7

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

328-37

issue

eissn

0300-8428

issn

1435-1803

journal_volume

pub_type

杂志文章

在线工具

Activation of distinct signal transduction pathways in hypertrophied hearts by pressure and volume overload.