Abstract：
:We studied TTX-sensitive Na channels in dissociated single ventricular cells from neonatal rats using the patch clamp method for single channel and whole cell recording. In both cases, slowly inactivating or window currents were observed that decayed in a biexponential fashion. Customary models of Na current kinetics such as the Hodgkin-Huxley model attribute activation to a process that is much faster than inactivation. The model of Aldrich, Corey and Stevens(1), says that inactivation is fast and final and activation is dispersed. We found activation too associated, deactivation too quick, and reopenings too frequent to accept this model for cardiac Na channels. We also found that the predominant set of single Na channels had one open state and two inactivated states. Rarely, a second set of Na channels having about 2/3 the conductance and mean open time of the predominant set was found.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Wilson DL,Lacerda AE,Kunze DL,Brown AMsubject
Has Abstractpub_date
1985-01-01 00:00:00pages
61-4eissn
0300-8428issn
1435-1803journal_volume
80 Suppl 2pub_type
杂志文章abstract：:At 36 degrees C and 2 mM [Ca2+]o, single guinea-pig ventricular myocytes were voltage clamped with patch electrodes. When paired pulsing had potentiated the contraction to the maximum, the cells were shock-frozen for electron probe microanalysis (EPMA). Shock-freezing was timed at the end of diastole (-80 mV) or at di...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-30769-4_9
更新日期：1991-01-01 00:00:00
abstract：:Atherosclerotic coronary arteries are more calcified in patients with than without chronic kidney disease (CKD). We addressed the potential for coronary microvascular obstruction in patients with and without CKD during stenting for saphenous vein aorto-coronary graft (SVG) stenosis under protection with a distal occlu...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0303-3
更新日期：2012-11-01 00:00:00
abstract：:Receptor for advanced glycation end products (RAGE) is associated with inflammation and the progression of cardiovascular diseases. The current study tested the hypothesis that RAGE is involved in the pathogenesis of aortic valve (AV) calcification. Pioglitazone attenuated AV calcification in experimental hypercholest...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0306-0
更新日期：2012-11-01 00:00:00
abstract：:Impaired nitric oxide (NO)-soluble guanylate cyclase (sGC)-cGMP signaling is involved in the pathogenesis of ischemic heart diseases, yet the impact of long-term sGC activation on progressive cardiac remodeling and heart failure after myocardial infarction (MI) has not been explored. Moreover, it is unknown whether st...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-014-0421-1
更新日期：2014-07-01 00:00:00
abstract：:Adult epicardial cells are required for endogenous cardiac repair. After myocardial injury, they are reactivated, undergo epithelial-to-mesenchymal transformation (EMT) and migrate into the injured myocardium where they generate various cell types, including coronary smooth muscle cells and cardiac interstitial fibrob...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-011-0181-0
更新日期：2011-09-01 00:00:00
abstract：:Increased afterload results in 'pathological' cardiac hypertrophy, the most important risk factor for the development of heart failure. Current in vitro models fall short in deciphering the mechanisms of hypertrophy induced by afterload enhancement. The aim of this study was to develop an experimental model that allow...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0307-z
更新日期：2012-11-01 00:00:00
abstract：BACKGROUND:Reopening of the infarct-related coronary artery is the treatment of choice in the clinical setting of acute myocardial infarction. Nevertheless the removal of the total occlusion obtained either by thrombolysis or by primary angioplasty is followed by the ischemia/reperfusion sequelae. One of many proposed ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950200049
更新日期：2002-09-01 00:00:00
abstract：:There is no doubt that major advances have been made in cardiac electrophysiology using single heart cells. Accurate analysis of rapid inward sodium current and the second inward current carried by calcium has resulted in a major re-examination of the steps involved in excitation-contraction coupling in the heart. Fut...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-11041-6_17
更新日期：1985-01-01 00:00:00
abstract：:Receptor binding studies usually show heterogenic characteristics of the binding isotherm. Scatchard transformation for graphical linearization of the data presentation affects the data inhomogeneously with respect to the experimental error. Thus a non-linear fitting procedure should be preferred for data analysis. Fu...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-642-72376-6_2
更新日期：1984-01-01 00:00:00
abstract：:Nitric oxide (NO) exerts several effects on myocardial contraction, including enhancement of relaxation and diastolic function, modulation of beta-adrenergic inotropic responses, and inotropic effects in the absence of agonist pre-stimulation. Different effects have been observed in different species and preparations,...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950050096
更新日期：1998-08-01 00:00:00
abstract：:Using an isolated rat heart preparation (Langendorff perfusion, perfusion pressure 100 cm H2O) the response of the hypertrophied heart (spontaneous hypertensive rats lv/bw ratio 3.6 +/- 0.5) to global normothermic (30 min) and hypothermic (25 degrees C, 120 min) ischemic and cardioplegic arrest and reperfusion (30 min...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-11374-5_12
更新日期：1986-01-01 00:00:00
abstract：:It is commonly thought that the optimal method for intracoronary administration of cells is to stop coronary flow during cell infusion, in order to prolong cell/vascular wall contact, enhance adhesion, and promote extravasation of cells into the interstitial space. However, occlusion of a coronary artery with a balloo...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-015-0503-8
更新日期：2015-09-01 00:00:00
abstract：:Carotid baroreceptor stimulation (CBS) has been shown to improve cardiac dysfunction and pathological structure remodelling. This study aimed to investigate the effects of CBS on the ventricular electrophysiological properties in canines with chronic heart failure (CHF). Thirty-eight beagles were randomized into contr...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-019-0750-1
更新日期：2019-09-09 00:00:00
abstract：:The focal adhesion and microfilament-associated protein VASP is a major substrate of both cAMP- and cGMP-dependent protein kinase in intact human platelets. The recent elucidation of the primary VASP structure and identity of VASP binding proteins suggest that VASP is an important component of focal contacts which lin...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788712
更新日期：1996-09-01 00:00:00
abstract：:Cardiac glycosides like ouabain are used in the therapy of heart failure and atrial fibrillation. They exert a positive inotropic effect on cardiomyocytes by inhibiting the plasma membrane sodium pump (Na,K-ATPase), decreasing the Ca-extrusion by the sarcolemmal cardiac sodium/calcium exchanger (NCX) and increasing th...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-003-0396-9
更新日期：2003-03-01 00:00:00
abstract：:Sarcoplasmic reticulum (SR) from left ventricles of rats that developed spontaneous hypertension was studied in vitro. Similar increases of left ventricular mass were found when grouping the animals into mild and severe hypertensives (average systolic arterial pressure of 168 +/- 4 and 202 +/- 6 mmHg, respectively). T...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907362
更新日期：1988-05-01 00:00:00
abstract：:A model of vascular endothelial cell is proposed to describe the mechanisms by which cytosolic calcium (Cai) is modulated and endothelium-derived relaxing factor (EDRF) and prostacyclin (PGI2) are released when the cell is stimulated by agonist. The intracellular Ca2+ store of the model cell is comprised of a superfic...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00796518
更新日期：1992-07-01 00:00:00
abstract：:Implicit in attempts to characterize and purify biologically active factors is the premise that the bioassay system employed will show a progressive increase in the response as the concentration of the responsible factor increases. We employed the hamster cheek pouch to assay the neovascularization potential of growth...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907406
更新日期：1986-05-01 00:00:00
abstract：:This study aimed to analyze the role of endothelial progenitor cell (EPC)-derived angiogenic factors and chemokines in the multistep process driving angiogenesis with a focus on the recently discovered macrophage migration inhibitory factor (MIF)/chemokine receptor axis. Primary murine and murine embryonic EPCs (eEPCs...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0310-4
更新日期：2013-01-01 00:00:00
abstract：:An ischaemia-like state in cultured heart cells has been obtained by markedly restricting the volume of extracellular medium combined with total deprivation of oxygen (anoxia) and glucose. Cellular injury, as reflected by the release of both cytoplasmic and lysosomal enzymes was significantly greater than during anoxi...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:The stroke volume at rest of 81 test subjects with no evident cardiac or circulatory dysfunction was determined by impedance cardiography and the measurements were repeated after 11 weeks. In order to attain comparable and reproducible conditions, 44 test subjects were chosen who showed heart rates at rest not higher ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906800
更新日期：1978-11-01 00:00:00
abstract：:A computer model is presented for simulation of the spread of activation and repolarization in ventricular myocardium. The program calculating the activation sequence is based on an algorithm similar to Huygen's principle of constructing wavefronts. Physiological parameters of the heart, such as areas of early activat...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02650875
更新日期：1989-07-01 00:00:00
abstract：:In order to clarify the genesis of the characteristic pressure and flow pulse contours of the common carotid artery of man, at first transcutaneous pulse recordings were carried out on healthy resting subjects during breathing of room air and of a mixture of 95% O2 and 5% CO2 (Carbogen). The latter was used to cause v...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907041
更新日期：1977-11-01 00:00:00
abstract：:Because of the close anatomical association between the ventricles, the volume of one ventricle can directly affect the volume and pressure within the other ventricle. To study the mechanical coupling between the ventricles, we modeled the right and left ventricles as a two-compartment model with right wall (Crw), sep...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907759
更新日期：1986-09-01 00:00:00
abstract：:Hypertrophic cardiomyopathy (HCM) is a leading cause of heart failure and sudden death in adolescents and young adults. Recently, the role of the Hippo/YAP pathway has been investigated in the pathogenesis of HCM, although the detailed molecular mechanisms largely remain elusive. In this study, we demonstrated an up-r...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-014-0435-8
更新日期：2014-01-01 00:00:00
abstract：:It has been investigated whether calcium- and verapamil-dependent sensitivities of carotis baroreceptors also exist in man. To answer this question, we pre- and postoperatively measured changes in heart rate during carotid sinus stimulation before and after intravenous administration of 5 mg verapamil in 23 patients w...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00795058
更新日期：1992-09-01 00:00:00
abstract：OBJECTIVE:The clinical usefulness of class III antiarrhythmic drugs for the treatment of tachyarrhythmias is limited by their potential proarrhythmic effects, mainly torsades-depointes (TdP). The goal of this experimental study was to develop an isolated whole-heart model exhibiting typical characteristics of class III...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950050073
更新日期：1998-04-01 00:00:00
abstract：:The endothelin (ET) family of peptides have potent vascular, cardiac and renal actions which may be of pathophysiological importance in congestive heart failure (CHF). In vivo studies with selective and non-selective ET receptor antagonists are required to clarify the role of ET in the pathophysiology of CHF and deter...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/BF00810520
更新日期：1996-01-01 00:00:00
abstract：:The effect of isoproterenol perfusion on cAMP levels and phosphorylase activity was investigated in the spontaneously hypertensive rat (SHR) and Kyoto Wistar normotensive control rat (WKY) heart. The basal force of contraction in physiological salt solution perfused hearts was comparable between SHR and WKY. However, ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907217
更新日期：1983-11-01 00:00:00
abstract：:In Langendorff-perfused guinea pig hearts, local myocardial perfusion was evaluated by analysis of FITC-dextran-150 elution kinetics after bolus injection. Locally estimated half-times of the monoexponential elution curves showed good correlation with global undisturbed coronary flow. After ligation of the left anteri...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01935807
更新日期：1984-01-01 00:00:00
