Abstract:
:It is commonly thought that the optimal method for intracoronary administration of cells is to stop coronary flow during cell infusion, in order to prolong cell/vascular wall contact, enhance adhesion, and promote extravasation of cells into the interstitial space. However, occlusion of a coronary artery with a balloon involves serious risks of vascular damage and/or dissection, particularly in non-stented segments such as those commonly found in patients with heart failure. It remains unknown whether the use of the stop-flow technique results in improved donor cell retention. Acute myocardial infarction was produced in 14 pigs. One to two months later, pigs received 10 million indium-111 oxyquinoline (oxine)-labeled c-kit(pos) human cardiac stem cells (hCSCs) via intracoronary infusion with (n = 7) or without (n = 7) balloon inflation. Pigs received cyclosporine to prevent acute graft rejection. Animals were euthanized 24 h later and hearts harvested for radioactivity measurements. With the stop-flow technique, the retention of hCSCs at 24 h was 5.41 ± 0.80 % of the injected dose (n = 7), compared with 4.87 ± 0.62 % without coronary occlusion (n = 7), (P = 0.60). When cells are delivered intracoronarily in a clinically relevant porcine model of chronic ischemic cardiomyopathy, the use of the stop-flow technique does not result in greater myocardial cell retention at 24 h compared with non-occlusive infusion. These results have practical implications for the design of cell therapy trials. Our observations suggest that the increased risk of complications secondary to coronary manipulation and occlusion is not warranted.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Keith MC,Tokita Y,Tang XL,Ghafghazi S,Moore JB 4th,Hong KU,Elmore JB,Amraotkar AR,Guo H,Ganzel BL,Grubb KJ,Flaherty MP,Vajravelu BN,Wysoczynski M,Bolli Rdoi
10.1007/s00395-015-0503-8subject
Has Abstractpub_date
2015-09-01 00:00:00pages
503issue
5eissn
0300-8428issn
1435-1803journal_volume
110pub_type
杂志文章abstract::The molecular mechanisms through which ghrelin exerts its cardioprotective effects during cardiac remodeling post-myocardial infarction (MI) are poorly understood. The aim of this study was to investigate whether the cardioprotection mechanisms are mediated by modulation of JAK/STAT signaling and what triggers this mo...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-018-0671-4
更新日期:2018-02-01 00:00:00
abstract::Studies were carried out in 39 barbiturate-anesthetized dogs to determine whether the renin-angiotensin system is important in control of hemodynamics and coronary flow during myocardial ischemia. Plasma renin activity (PRA) was 2.2 +/- 0.4 ng x ml-l x hr-1 immediately before coronary artery occlusion (CAO) and increa...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906463
更新日期:1983-09-01 00:00:00
abstract::The effect of isoproterenol perfusion on cAMP levels and phosphorylase activity was investigated in the spontaneously hypertensive rat (SHR) and Kyoto Wistar normotensive control rat (WKY) heart. The basal force of contraction in physiological salt solution perfused hearts was comparable between SHR and WKY. However, ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907217
更新日期:1983-11-01 00:00:00
abstract::The PKC hypothesis and its variants are attractive but unproved. The chief difficulty is the lack of an objective means to assess whether or not PKC has been activated in hearts preconditioned in vivo with ischemia or in hearts preconditioned by pharmacologic means. The strongest evidence is supporting the PKC hypothe...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00797206
更新日期:1997-01-01 00:00:00
abstract::Myocardial stunning, a transient contractile dysfunction that appears following a brief period of ischemia, is at least partly due to the production of oxygen-derived free radicals. The objective of the present study was to determine whether the Ginkgo biloba extract EGb761, which has antioxidant properties in vitro, ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-003-0380-4
更新日期:2003-02-01 00:00:00
abstract::Atherosclerotic coronary arteries are more calcified in patients with than without chronic kidney disease (CKD). We addressed the potential for coronary microvascular obstruction in patients with and without CKD during stenting for saphenous vein aorto-coronary graft (SVG) stenosis under protection with a distal occlu...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-012-0303-3
更新日期:2012-11-01 00:00:00
abstract::This study was designed to evaluate whether the decreased early survival rate of diabetic rats submitted to acute experimental myocardial infarction can be improved by pretreatment with lidocaine. Male Wistar rats (+/- 210 g) were rendered diabetic with i.v. injection of streptozotocin (50 mg/kg), and only those prese...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907132
更新日期:1990-07-01 00:00:00
abstract:OBJECTIVE:Cardiac stunning is the consequence of a brief cardiac ischemia. The underlying mechanism is not completely understood. METHODS:Here we induced cardiac transient ischemia in conscious instrumented dogs by means of an occluder in the left anterior descending coronary artery (LAD). Contractile performance, mon...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-005-0577-9
更新日期:2006-05-01 00:00:00
abstract::The influence of cardiac cholinergic activation was studied in rats and cats on the induction and maintenance of ventricular fibrillation (VF). Acetylcholine (ACH 2-25 micrograms/kg), in doses which did not cause bradycardia or hypotension, induced appearance of spontaneous VF (duration 2-60 sec.) in 9/20 rats which h...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908386
更新日期:1984-11-01 00:00:00
abstract::If everything happens strictly according to the natural laws, which meet extremum principles, in what way can the possibility for life be characterised, so that the optimization processes of evolution can take place? Is it legitimate to "enlarge" the natural laws by certain laws of conservation? The question then aris...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Myocardial ischemia and infarction are the most common substrates for life-threatening ventricular tachyarrhythmias. Experimental and clinical evidence suggests that enhanced activity of the sympathetic nervous system plays an important role in the genesis of ischemia-related arrhythmias. In animal experiments, beta-b...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/978-3-662-11038-6_24
更新日期:1990-01-01 00:00:00
abstract::Transient receptor potential melastatin-7 (TRPM7) channels have been recently reported in human atrial fibroblasts and are believed to mediate fibrogenesis in human atrial fibrillation. The present study investigates whether TRPM7 channels are expressed in human atrial myocytes using whole-cell patch voltage-clamp, RT...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-012-0282-4
更新日期:2012-09-01 00:00:00
abstract::The effect of 0.1 mg/kg Verapamil on the extent of the ischemic area following ligation of a branch of the left descending coronary artery was studied in anesthetized open chest dogs. The sum of the ST-segment elevation during the entire period of occlusion in the epicardial ECG was 15 mV in control infarction compare...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01905620
更新日期:1975-03-01 00:00:00
abstract::Coronary vasomotion of normal, stenotic, and poststenotic vessel segments was studied in 18 patients with coronary artery disease at rest, during submaximal bicycle exercise, and 5 min after sublingual nitroglycerin or oral isosorbide-dinitrate (ISDN) spray. Patients were divided into two groups: group 1 consisted of ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11038-6_28
更新日期:1990-01-01 00:00:00
abstract::Conflicting data on the inotropic effect of insulin are present in the literature suggesting a positive inotropic property or no inotropic effect or even a negative influence. To clarify the reason for these diverging findings, dose-response curves of insulin have been performed in isolated working rat and guinea pig ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-002-0350-2
更新日期:2002-07-01 00:00:00
abstract::Recent attention has focused on the concept that mitochondrial dynamics-that is, the balance between mitochondrial fusion and fission (fragmentation)-may play a pivotal role in determining cell fate in the setting of myocardial ischemia-reperfusion injury. In this regard, there is an emerging consensus that: (1) ische...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-016-0578-x
更新日期:2016-09-01 00:00:00
abstract::Disregarding the influence of thickness on elevated strength of isolated preparations inevitably leads to erroneous tension-frequency relations, especially in the range of high frequencies. Thus, much of the confusion in interpreting the atypical negative staircase phenomenon of the rat heart is due to this. In view o...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11289-2_25
更新日期:1987-01-01 00:00:00
abstract::The aim of the present study was to evaluate the expression of hedgehog (Hh) signaling molecules and the chemotactic activity of Sonic hedgehog (Shh) in monocytes from control (CTR) and diabetic patients with or without coronary artery disease (CAD). Previously several studies demonstrated that exogenous administratio...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-009-0047-x
更新日期:2010-01-01 00:00:00
abstract::To understand the effect of transcription regulation in modulating cardiac aging, we sought to study the role of serum response factor (SRF), a key transcription factor in the heart that is normally increased with senescence and also in congestive heart failure. A Tet-Off gene expression system was used for cardiac-sp...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-006-0634-z
更新日期:2007-05-01 00:00:00
abstract::Hemodynamics and myocardial contractility were evaluated in five unanesthetized calves during acute hypocapnic and isocapnic hypoxia and during acute hypocapnic hypoxia with beta-adrenergic blockade. Both hypocapnic and isocapnic hypoxia, with mean PaO2 levels of 33.1 and 39.1 mm Hg respectively, produced a decline in...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01909762
更新日期:1976-09-01 00:00:00
abstract::32 patients were studied by His-bundle recordings to examine the extent to which frequency and autonomic nervous system influence the conduction velocity in the subdivisions (atrium, AV-node, His-Purkinje system) of the normal PR-interval. The measurements were performed during sinus rhythm and three electrically indu...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::The influence of changes in pre- and afterload, heart frequency and inotropic state on the difference between right ventricular dP/dtmax measured isovolumically and auxotonically has been investigated in canine heart-lung preparations. Isovolumic systoles were obtained by occluding the pulmonary artery during diastole...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01909767
更新日期:1976-09-01 00:00:00
abstract::Adult epicardial cells are required for endogenous cardiac repair. After myocardial injury, they are reactivated, undergo epithelial-to-mesenchymal transformation (EMT) and migrate into the injured myocardium where they generate various cell types, including coronary smooth muscle cells and cardiac interstitial fibrob...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-011-0181-0
更新日期:2011-09-01 00:00:00
abstract::The renin angiotensin system and endothelium-derived vasoactive substances are both important regulators of vascular tone. Recent evidence suggests that the two systems may be tightly interconnected and drugs interfering with one system may also affect the other. Beside the circulating renin angiotensin system, a vasc...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/978-3-642-72497-8_2
更新日期:1993-01-01 00:00:00
abstract::The effects of the potassium channel openers (KCO), cromakalim or pinacidil, were evaluated in an anesthetized porcine model of pacing- and ischemia-induced ventricular fibrillation (VF). Hearts were paced at 180 bpm and the left anterior descending coronary artery was occluded until VF was induced. Reproducible times...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788735
更新日期:1994-03-01 00:00:00
abstract::Atrial fibrillation (AF) is the most prevalent cardiac arrhythmia with a strong genetic component. Molecular pathways involving the homeodomain transcription factor Shox2 control the development and function of the cardiac conduction system in mouse and zebrafish. Here we report the analysis of human SHOX2 as a potent...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-016-0557-2
更新日期:2016-05-01 00:00:00
abstract::In dog myocardium, the changes in the levels of creatine phosphate, inorganic phosphate, ATP, ADP, AMP, adenosine and inosine with 8 min of ischemia and subsequent reperfusion for 2, 4, 8, 16 and 32 min have been followed. Creatine phosphate and inorganic phosphate recovered completely within a few minutes as did the ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907461
更新日期:1986-07-01 00:00:00
abstract::It is generally believed that the increased contractility and tachycardia of the hyperthyroid heart are a result of thyroid hormone-induced alterations of the mechanical and electrical properties of the heart, respectively. We compared the contractility (dP/dtmax) and the spontaneous beating rate of hyperthyroid and e...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906958
更新日期:1988-11-01 00:00:00
abstract:AIMS:We studied the capability of heart rate (HR) adjusted change in multichannel magnetocardiogram (MCG) to detect exercise-induced ischemia. METHODS AND RESULTS:The MCG and 12-lead ECG were recorded simultaneously during supine exercise testing in 17 healthy controls and 24 patients with single vessel coronary arter...
journal_title:Basic research in cardiology
pub_type: 临床试验,杂志文章
doi:10.1007/s395-002-8391-y
更新日期:2002-01-01 00:00:00
abstract::The pathogenesis of atherosclerosis is a multifactorial process. A possible anti-atherosclerotic drug should therefore interfere with different targets that are important during the development of an atherosclerotic lesion. Two of the early events are the activated migration and proliferation of arterial smooth muscle...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907976
更新日期:1989-05-01 00:00:00