Reduced serine-16 and threonine-17 phospholamban phosphorylation in stunning of conscious dogs: no evidence for any involvement of protein kinase A or protein phosphatases.

Abstract:

OBJECTIVE:Cardiac stunning is the consequence of a brief cardiac ischemia. The underlying mechanism is not completely understood. METHODS:Here we induced cardiac transient ischemia in conscious instrumented dogs by means of an occluder in the left anterior descending coronary artery (LAD). Contractile performance, monitored by ultrasound crystals, was reduced during and after ischemia in the LAD area. For control in the same animals cardiac performance was measured in the area of left circumflex coronary artery (Ramus circumflexus, RCx). In the RCx area, no decline in contractility was noted. Tissue was obtained from stunned LAD area and from control areas (RCx). RESULTS:Phospholamban phosphorylation on both serine-16 and threonine-17 was reduced in LAD areas compared to RCx areas. Reduced phosphorylation of PLB is known to inhibit cardiac contractility. While phosphorylation of PLB was reduced, the activity of the appropriate protein phosphatases and protein kinases was not different between tissue obtained from LAD or RCx areas. CONCLUSION:Reduced formation of cAMP might underlie the contractile dysfunction in myocardial stunning.

journal_name

Basic Res Cardiol

authors

Weber T,Neumann J,Meissner A,Grosse Hartlage M,Van Aken H,Hanske G,Schmitz W,Boknik P

doi

10.1007/s00395-005-0577-9

keywords:

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

253-60

issue

3

eissn

0300-8428

issn

1435-1803

journal_volume

101

pub_type

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