Abstract:
:Recent publications shown mitochondrial localization of the enzyme nitric oxide synthase (NOS) in a number of tissues. However, conflicting results about mitochondrial NOS (mtNOS) immunoreactivity and enzymatic activity are available to date in the literature. In this study we purified mitochondria from rat hearts and analysed these preparations for NOS immunoreactivity and activity, showing the presence of either a constitutive (the endothelial isoform) and an inducible NOS immunoreactivity. A basal NOS activity (64.2 +/- 5.1 pmol/mg protein/30 min) was detectable. 1 mM N(G)-Monomethyl-L-arginine (L-NMMA), a competitive inhibitor of all NOS isoforms, caused a drop in NOS activity to 33.8 +/- 1.9 pmol/mg protein/30 min. Simultaneous administration of 10 micro M (S)-2-amino-(1-iminoethylamino)-5- thiopentanoic acid (GW274150), a specific NOS2 inhibitor, together with removal of Ca(2+) and calmodulin (CaM) from the assay buffers, known to interfere with the activity of constitutive NOS isoforms, caused a reduction in NOS activity (17.4 +/- 1.2 pmol/mg protein/30 min). 10 micro M GW274150 reduced NOS activity to 41.6 +/- 4 pmol/mg protein/30 min, while Ca(2+)/CaM withdrawal reduced basal NOS activity to 45.8 +/- 5 pmol/mg protein/30 min. This dual mtNOS machinery is suggested to be involved in modulating cardiac O(2) consumption in different (patho)physiological conditions.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Zanella B,Giordano E,Muscari C,Zini M,Guarnieri Cdoi
10.1007/s00395-003-0454-3keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
159-64issue
3eissn
0300-8428issn
1435-1803journal_volume
99pub_type
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journal_title:Basic research in cardiology
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journal_title:Basic research in cardiology
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doi:10.1007/s00395-005-0577-9
更新日期:2006-05-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/BF02070160
更新日期:1983-07-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Basic research in cardiology
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doi:
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journal_title:Basic research in cardiology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2001-07-01 00:00:00