Abstract:
:Two novel Gd-based contrast agents (CAs) for the molecular imaging of matrix metalloproteinases (MMPs) were synthetized and characterized in vitro and in vivo. These probes were based on the PLG*LWAR peptide sequence, known to be hydrolyzed between Gly and Leu by a broad panel of MMPs. A Gd-DOTA chelate was conjugated to the N-terminal position through an amide bond, either directly to proline (compd Gd-K11) or through a hydrophilic spacer (compd Gd-K11N). Both CA were made strongly amphiphilic by conjugating an alkyl chain at the C-terminus of the peptide sequence. Gd-K11 and Gd-K11N have a good affinity for β-cyclodextrins (K(D) 310 and 670 µ m respectively) and for serum albumin (K(D) 350 and 90 µ m respectively), and can be efficiently cleaved in vitro at the expected site by MMP-2 and MMP-12. Upon MMP-dependent cleavage, the CAs lose the C-terminal tetrapeptide and the alkyl chain, thus undergoing to an amphiphilic-to-hydrophilic transformation that is expected to alter tissue pharmacokinetics. To prove this, Gd-K11 was systemically administered to mice bearing a subcutaneous B16.F10 melanoma, either pre-treated or not with the broad spectrum MMP inhibitor GM6001 (Ilomastat). The washout of the Gd-contrast enhancement in MR images was significantly faster for untreated subjects (displaying MMP activity) with respect to treated ones (MMP activity inhibited). The washout kinetics of Gd-contrast enhancement from the tumor microenvironment could be then interpreted in terms of the local activity of MMPs.
journal_name
Contrast Media Mol Imagingjournal_title
Contrast media & molecular imagingauthors
Gringeri CV,Menchise V,Rizzitelli S,Cittadino E,Catanzaro V,Dati G,Chaabane L,Digilio G,Aime Sdoi
10.1002/cmmi.478subject
Has Abstractpub_date
2012-03-01 00:00:00pages
175-84issue
2eissn
1555-4309issn
1555-4317journal_volume
7pub_type
杂志文章abstract::Melanoma is the most dangerous form of skin cancer and its incidence is rising each year. Because the current methods of diagnosis based on the visual aspect of the tumor show limitations, several new techniques are emerging to help in this diagnosis, amongst which are magnetic resonance imaging (MRI) and electron par...
journal_title:Contrast media & molecular imaging
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abstract::The aim of this work was to investigate the MRI findings on tumor xenografts induced in nude mice by the inoculation of human pancreatic cancer cells labeled with superparamagnetic iron oxide (SPIO), and to monitor the kinetics of SPIO distribution in tumor xenografts. The labeled cancer cells were subcutaneously inoc...
journal_title:Contrast media & molecular imaging
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abstract::Magnetic fluid hyperthermia (MFH) with chemically synthesized nanoparticles is currently used in clinical trials as it destroys tumor cells with an extremely localized deposition of thermal energy. In this paper, we investigated an MFH protocol based on magnetic nanoparticles naturally produced by magnetotactic bacter...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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abstract::For in vivo applications of magnetically labeled stem cells, biological effects of the labeling procedure have to be precluded. This study evaluates the effect of different ferucarbotran cell labeling protocols on chondrogenic differentiation of human mesenchymal stem cells (hMSC) as well as their implications for MR ...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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abstract::Advances in regenerative medicine are rapidly transforming healthcare. A cornerstone of regenerative medicine is the introduction of cells that were grown or manipulated in vitro. Key questions that arise after these cells are re-introduced are: whether these cells are localized in the appropriate site; whether cells ...
journal_title:Contrast media & molecular imaging
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abstract:Aim:To evaluate reproducibility and stability of radiomic features as effects of the use of different volume segmentation methods and reconstruction settings. The potential of radiomics in really capturing the presence of heterogeneous tumor uptake and irregular shape was also investigated. Materials and Methods:An an...
journal_title:Contrast media & molecular imaging
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abstract::The over-expression of sialic acid on the surface of cancer cells compared with normal ones makes this nine-carbon sugar an attractive biomarker for molecular diagnosis and therapy. Here, we describe a study on the molecular recognition of sialic acid end groups on the surface of human glioma cells by (160)Tb-DTPA-EN(...
journal_title:Contrast media & molecular imaging
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abstract::Overexpression of the chemokine-4 receptor (CXCR4) in brain tumors is associated with high cancer cell invasiveness. Recently, we reported the preclinical evaluation of 99mTc-CXCR4-L (cyclo-D-Tyr-D-[NMe]Orn[EDDA-99mTc-6-hydrazinylnicotinyl]-Arg-NaI-Gly) as a SPECT radioligand capable of specifically detecting the CXCR...
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abstract::An experimental setup consisting of a hollow fiber module (HFM) was developed for the in vitro study of contrast agent (CA) permeability. Controllable flow and known fiber characteristics allowed permeability studies under well-defined conditions with CAs of different molecular weight (MW). In the MRI experiments perf...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.262
更新日期:2009-03-01 00:00:00
abstract::In this study we report the development of a bioconjugate between superparamagnetic iron oxide nanoparticles and Phl p5a (one of the major allergens from grass pollen). The bioconjugate also contains an optical probe (Alexa647) conjugated to the nanoparticle via biotin-streptavidin association. We show that this conju...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1466
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abstract::In vivo molecular imaging with targeted MRI contrast agents will require sensitive methods to quantify local concentrations of contrast agent, enabling not only imaging-based recognition of pathological biomarkers but also detection of changes in expression levels as a consequence of disease development, therapeutic i...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.398
更新日期:2011-01-01 00:00:00
abstract::Physicians and scientists use a broad spectrum of terms to classify contrast media (CM)-induced adverse reactions. In particular, the designation of hypersensitivity reactions is quite varied. Consequently, comparisons of different papers dealing with this subject are difficult or even impossible. Moreover, general de...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1705
更新日期:2016-11-01 00:00:00
abstract::Antibody-labeled gold nanoparticles represent a promising novel tool regarding cancer imaging and therapy. Nevertheless, the characterization of biodistribution of such immunonanocarriers has been poorly documented. In this study, the biodistribution of (89)Zr-labeled cetuximab before and after the coupling reaction t...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1539
更新日期:2013-09-01 00:00:00
abstract:Objective:We aimed to propose an automatic method based on Support Vector Machine (SVM) and Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) to segment the tumor lesions of head and neck cancer (HNC). Materials and Methods:120 DCE-MRI samples were collected. Five curve features and two principal componen...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1155/2017/8612519
更新日期:2017-09-07 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,评审
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更新日期:2017-11-21 00:00:00
abstract:Objective:To qualitatively and quantitatively compare the contrast-enhanced magnetic resonance angiography (MRA) and magnetic resonance imaging (MRI) in one-stop shop of abdominal imaging with Gadobutrol and Gd-DTPA at equimolar doses of gadolinium. Materials and Methods:This was a prospective designed, multiple cente...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,多中心研究,随机对照试验
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abstract::Photoacoustic imaging is an emerging imaging modality for noninvasive detection of tissue structural and functional anomalies. Multifunctional microbubbles (MBs) and nanobubbles (NBs) are contrast agents integrating multiple disease-targeting, imaging and therapeutic functions. Multifunctional MBs and NBs represent an...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,评审
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更新日期:2011-09-01 00:00:00
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
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doi:10.1002/cmmi.289
更新日期:2009-09-01 00:00:00
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
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abstract::Chemical exchange saturation transfer (CEST) imaging is an emerging MRI technique relying on the use of endogenous or exogenous molecules containing exchangeable proton pools. The heterogeneity of the water resonance frequency offset plays a key role in the occurrence of artifacts in CEST-MR images. To limit this draw...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.240
更新日期:2008-07-01 00:00:00
abstract::The gonadotropin-releasing hormone (GnRH) receptor is overexpressed in the majority of tumors of the human reproductive system. The purpose of this study was to develop an 18F-labeled peptide for tumor GnRH receptor imaging. In this study, the GnRH (pGlu1-His2-Trp3-Ser4-Tyr5-Gly6-Leu7-Arg8-Pro9-Gly10-NH2) peptide anal...
journal_title:Contrast media & molecular imaging
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更新日期:2019-03-07 00:00:00
abstract::An observational study was set up to assess the feasibility of [18F]FPRGD2 PET/CT for imaging patients with multiple myeloma (MM) and to compare its detection rate with low dose CT alone and combined [18F]NaF/[18F]FDG PET/CT images. Four patients (2 newly diagnosed patients and 2 with relapsed MM) were included and un...
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更新日期:2017-07-27 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1473
更新日期:2012-09-01 00:00:00
abstract::[This corrects the article DOI: 10.1155/2017/7368384.]. ...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,已发布勘误
doi:10.1155/2018/2165693
更新日期:2018-08-23 00:00:00
abstract::The aim of the present study was to test alginate gels of different compositions as a system for controlled release of manganese ions (Mn(2+)) for application in manganese-enhanced MRI (MEMRI), in order to circumvent the challenge of achieving optimal MRI resolution without resorting to high, potentially cytotoxic dos...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
abstract::Various contrast agents (Magnevist, Gadovist and Multihance) loaded into polymeric nanocapsules were synthesized by the inverse miniemulsion technique. The relaxivity of the resultant contrast agents was assessed at 1.5 T magnetic field strength. The ionic relaxivity of the contrast agents could at least be maintained...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.364
更新日期:2010-03-01 00:00:00