Abstract:
:In this study we report the development of a bioconjugate between superparamagnetic iron oxide nanoparticles and Phl p5a (one of the major allergens from grass pollen). The bioconjugate also contains an optical probe (Alexa647) conjugated to the nanoparticle via biotin-streptavidin association. We show that this conjugate has a range of features that makes it a very promising candidate to image the localization of this allergen in vivo: (a) upon conjugation to the iron oxide nanoparticles, the allergen retains its ability to interact with IgE antibodies; (b) the magnetic properties of the iron oxide core of this bioconjugate are suitable for MR imaging; and (c) Alexa647 fluorophore retains its emission properties once attached to the iron oxide nanoparticles, yielding a dual modality MRI-optical probe.
journal_name
Contrast Media Mol Imagingjournal_title
Contrast media & molecular imagingauthors
Herranz F,Schmidt-Weber CB,Shamji MH,Narkus A,Ruiz-Cabello J,Vilar Rdoi
10.1002/cmmi.1466subject
Has Abstractpub_date
2012-07-01 00:00:00pages
435-9issue
4eissn
1555-4309issn
1555-4317journal_volume
7pub_type
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