Abstract:
:Self-aggregation of dyes even at low concentrations poses a considerable challenge in preparing sufficiently bright molecular probes for in vivo imaging, particularly in the conjugation of near infrared cyanine dyes to polypeptides with multiple labeling sites. Such self-aggregation leads to a significant energy transfer between the dyes, resulting in severe quenching and low brightness of the targeted probe. To address this problem, we designed a novel type of dye with an asymmetrical distribution of charge. Asymmetrical distribution prevents the chromophores from π-stacking thus minimizing the energy transfer and fluorescence quenching. The conjugation of the dye to polypeptides showed only a small presence of an H-aggregate band in the absorption spectra and, hence, a relatively high quantum efficiency.
journal_name
Contrast Media Mol Imagingjournal_title
Contrast media & molecular imagingauthors
Zhegalova NG,He S,Zhou H,Kim DM,Berezin MYdoi
10.1002/cmmi.1585subject
Has Abstractpub_date
2014-09-01 00:00:00pages
355-62issue
5eissn
1555-4309issn
1555-4317journal_volume
9pub_type
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