The influence of ferucarbotran on the chondrogenesis of human mesenchymal stem cells.

Abstract:

:For in vivo applications of magnetically labeled stem cells, biological effects of the labeling procedure have to be precluded. This study evaluates the effect of different ferucarbotran cell labeling protocols on chondrogenic differentiation of human mesenchymal stem cells (hMSC) as well as their implications for MR imaging. hMSC were labeled with ferucarbotran using various protocols: cells were labeled with 100 microg Fe/ml for 4 and 18 h and additional samples were cultured for 6 or 12 days after the 18 h labeling. Supplementary samples were labeled by transfection with protamine sulfate. Iron uptake was quantified by ICP-spectrometry and labeled cells were investigated by transmission electron microscopy and by immunostaining for ferucarbotran. The differentiation potential of labeled cells was compared with unlabeled controls by staining with Alcian blue and Hematoxylin and Eosin, then quantified by measurements of glucosaminoglycans (GAG). Contrast agent effect at 3 T was investigated on days 1 and 14 of chondrogenic differentiation by measuring signal-to-noise ratios on T(2)-SE and T(2)*-GE sequences. Iron uptake was significant for all labeling protocols (p < 0.05). The uptake was highest after transfection with protamine sulfate (25.65 +/- 3.96 pg/cell) and lowest at an incubation time of 4 h without transfection (3.21 +/- 0.21 pg/cell). While chondrogenic differentiation was decreased using all labeling protocols, the decrease in GAG synthesis was not significant after labeling for 4 h without transfection. After labeling by simple incubation, chondrogenesis was found to be dose-dependent. MR imaging showed markedly lower SNR values of all labeled cells compared with the unlabeled controls. This contrast agent effect persisted for 14 days and the duration of differentiation. Magnetic labeling of hMSC with ferucarbotran inhibits chondrogenesis in a dose-dependent manner when using simple incubation techniques. When decreasing the incubation time to 4 h, inhibition of chondrogenesis was not significant.

authors

Henning TD,Sutton EJ,Kim A,Golovko D,Horvai A,Ackerman L,Sennino B,McDonald D,Lotz J,Daldrup-Link HE

doi

10.1002/cmmi.276

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

165-73

issue

4

eissn

1555-4309

issn

1555-4317

journal_volume

4

pub_type

杂志文章
  • Novel Gd(III)-based probes for MR molecular imaging of matrix metalloproteinases.

    abstract::Two novel Gd-based contrast agents (CAs) for the molecular imaging of matrix metalloproteinases (MMPs) were synthetized and characterized in vitro and in vivo. These probes were based on the PLG*LWAR peptide sequence, known to be hydrolyzed between Gly and Leu by a broad panel of MMPs. A Gd-DOTA chelate was conjugated...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.478

    authors: Gringeri CV,Menchise V,Rizzitelli S,Cittadino E,Catanzaro V,Dati G,Chaabane L,Digilio G,Aime S

    更新日期:2012-03-01 00:00:00

  • Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies.

    abstract::MRI cell tracking is a promising technique to track various cell types (stem cells, tumor cells, etc.) in living animals. Usually, cells are incubated with iron oxides (T(2) contrast agent) in order to take up the particles before being injected in vivo. Iron oxide quantification is important in such studies for valid...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.497

    authors: Danhier P,De Preter G,Boutry S,Mahieu I,Leveque P,Magat J,Haufroid V,Sonveaux P,Bouzin C,Feron O,Muller RN,Jordan BF,Gallez B

    更新日期:2012-05-01 00:00:00

  • Influence of cell-internalization on relaxometric, optical and compositional properties of targeted paramagnetic quantum dot micelles.

    abstract::Quantum dot micelles (pQDs) with a paramagnetic coating are promising nanoparticles for bimodal molecular imaging. Their bright fluorescence allows for optical detection, while their Gd payload enables visualization with contrast-enhanced MRI. A popular approach in molecular MRI is the targeting of abundantly expresse...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.411

    authors: Starmans LW,Kok MB,Sanders HM,Zhao Y,Donegá Cde M,Meijerink A,Mulder WJ,Grüll H,Strijkers GJ,Nicolay K

    更新日期:2011-03-01 00:00:00

  • Disruptive chemical doping in a ferritin-based iron oxide nanoparticle to decrease r2 and enhance detection with T1-weighted MRI.

    abstract::Inorganic doping was used to create flexible, paramagnetic nanoparticle contrast agents for in vivo molecular magnetic resonance imaging (MRI) with low transverse relaxivity (r2). Most nanoparticle contrast agents formed from superparamagnetic metal oxides are developed with high r2. While sensitive, they can have lim...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.1578

    authors: Clavijo Jordan MV,Beeman SC,Baldelomar EJ,Bennett KM

    更新日期:2014-09-01 00:00:00

  • Considerations for the clinical use of contrast agents for cellular MRI in regenerative medicine.

    abstract::Advances in regenerative medicine are rapidly transforming healthcare. A cornerstone of regenerative medicine is the introduction of cells that were grown or manipulated in vitro. Key questions that arise after these cells are re-introduced are: whether these cells are localized in the appropriate site; whether cells ...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章,评审

    doi:10.1002/cmmi.1547

    authors: Modo M,Kolosnjaj-Tabi J,Nicholls F,Ling W,Wilhelm C,Debarge O,Gazeau F,Clement O

    更新日期:2013-11-01 00:00:00

  • Comparison of 68Ga-DOTA-Siglec-9 and 18F-Fluorodeoxyribose-Siglec-9: Inflammation Imaging and Radiation Dosimetry.

    abstract::Sialic acid-binding immunoglobulin-like lectin 9 (Siglec-9) is a ligand of inflammation-inducible vascular adhesion protein-1 (VAP-1). We compared 68Ga-DOTA- and 18F-fluorodeoxyribose- (FDR-) labeled Siglec-9 motif peptides for PET imaging of inflammation. Methods. Firstly, we examined 68Ga-DOTA-Siglec-9 and 18F-FDR-S...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2017/7645070

    authors: Virtanen H,Silvola JMU,Autio A,Li XG,Liljenbäck H,Hellberg S,Siitonen R,Ståhle M,Käkelä M,Airaksinen AJ,Helariutta K,Tolvanen T,Veres TZ,Saraste A,Knuuti J,Jalkanen S,Roivainen A

    更新日期:2017-12-31 00:00:00

  • Preparation of 68Ga-PSMA-11 with a Synthesis Module for Micro PET-CT Imaging of PSMA Expression during Prostate Cancer Progression.

    abstract:Objective:To synthesize 68Ga-Glu-urea-Lys(Ahx)-HBED-CC (68Ga-PSMA-11) with a synthesis module and investigate PET-CT imaging to monitor PSMA expression during prostate cancer (PCa) progression and tumor growth in mice bearing subcutaneous PCa xenografts. Method:The radiochemical purity and stability of  68Ga-PSMA-11 w...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2018/8046541

    authors: Wang Y,Shao G,Wu J,Cui C,Zang S,Qiu F,Jia R,Wang Z,Wang F

    更新日期:2018-04-26 00:00:00

  • How to perform accurate and reliable measurements of longitudinal and transverse relaxation times of MRI contrast media in aqueous solutions.

    abstract::Knowledge of the longitudinal and transverse relaxation times (T(1) and T(2)) of water protons in an aqueous solution of an MRI contrast agent is essential for its characterization. These parameters can be measured at low field on low resolution spectrometers or at high field on high resolution spectrometers. The reli...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.294

    authors: Henoumont C,Laurent S,Vander Elst L

    更新日期:2009-11-01 00:00:00

  • Quantitative chemical exchange saturation transfer (qCEST) MRI--RF spillover effect-corrected omega plot for simultaneous determination of labile proton fraction ratio and exchange rate.

    abstract::Chemical exchange saturation transfer (CEST) MRI is sensitive to dilute proteins and peptides as well as microenvironmental properties. However, the complexity of the CEST MRI effect, which varies with the labile proton content, exchange rate and experimental conditions, underscores the need for developing quantitativ...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.1569

    authors: Sun PZ,Wang Y,Dai Z,Xiao G,Wu R

    更新日期:2014-07-01 00:00:00

  • Synthesis and in vitro evaluation of MR molecular imaging probes using J591 mAb-conjugated SPIONs for specific detection of prostate cancer.

    abstract::Carcinoma of the prostate is the most frequent diagnosed malignant tumor in men and is the second leading cause of cancer-related death in this group. The cure rate of prostate cancer is highly dependent on the stage of disease at the diagnosis and early detection is key to designing effective treatment strategies. Th...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.1514

    authors: Abdolahi M,Shahbazi-Gahrouei D,Laurent S,Sermeus C,Firozian F,Allen BJ,Boutry S,Muller RN

    更新日期:2013-03-01 00:00:00

  • Classification of acute and delayed contrast media-induced reactions: proposal of a three-step system.

    abstract::Physicians and scientists use a broad spectrum of terms to classify contrast media (CM)-induced adverse reactions. In particular, the designation of hypersensitivity reactions is quite varied. Consequently, comparisons of different papers dealing with this subject are difficult or even impossible. Moreover, general de...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.1475

    authors: Böhm I,Heverhagen JT,Klose KJ

    更新日期:2012-11-01 00:00:00

  • Diagnostic Performance of PET or PET/CT with Different Radiotracers in Patients with Suspicious Lung Cancer or Pleural Tumours according to Published Meta-Analyses.

    abstract:Purpose:Several meta-analyses have reported data about the diagnostic performance of positron emission tomography or positron emission tomography/computed tomography (PET or PET/CT) with different radiotracers in patients with suspicious lung cancer (LC) or pleural tumours (PT). This review article aims at providing an...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章,评审

    doi:10.1155/2020/5282698

    authors: Lococo F,Muoio B,Chiappetta M,Nachira D,Petracca Ciavarella L,Margaritora S,Treglia G

    更新日期:2020-04-25 00:00:00

  • Dynamic manganese-enhanced magnetic resonance imaging can detect chronic cryoinjury-induced infarction in pig hearts in vivo.

    abstract::The purpose was to investigate whether MnCl(2) can serve as an MRI contrast agent to detect chronic cryoinjury infarction in pigs in vivo and whether MnCl(2) causes significant hemodynamic disturbances. Hearts were subjected to a topical 2 min cryothermia to establish myocardial infarction (MI). Thereafter GdDTPA-enha...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.438

    authors: Yang Y,de Gervai PD,Sun J,Gruwel ML,Kupriyanov V

    更新日期:2011-11-01 00:00:00

  • Parameters Influencing PET Imaging Features: A Phantom Study with Irregular and Heterogeneous Synthetic Lesions.

    abstract:Aim:To evaluate reproducibility and stability of radiomic features as effects of the use of different volume segmentation methods and reconstruction settings. The potential of radiomics in really capturing the presence of heterogeneous tumor uptake and irregular shape was also investigated. Materials and Methods:An an...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2018/5324517

    authors: Gallivanone F,Interlenghi M,D'Ambrosio D,Trifirò G,Castiglioni I

    更新日期:2018-09-10 00:00:00

  • MR imaging of human pancreatic cancer xenograft labeled with superparamagnetic iron oxide in nude mice.

    abstract::The aim of this work was to investigate the MRI findings on tumor xenografts induced in nude mice by the inoculation of human pancreatic cancer cells labeled with superparamagnetic iron oxide (SPIO), and to monitor the kinetics of SPIO distribution in tumor xenografts. The labeled cancer cells were subcutaneously inoc...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.465

    authors: Wu CY,Pu Y,Liu G,Shao Y,Ma QS,Zhang XM

    更新日期:2012-01-01 00:00:00

  • Use of 18F-FDG-PET/CT for Retroperitoneal/Intra-Abdominal Soft Tissue Sarcomas.

    abstract:Rationale:To assess the diagnostic value of 18F-FDG-PET/CT for different retroperitoneal soft tissue sarcomas (STS) and other similar tumors. To analyze the predictive value of 18F-FDG-PET/CT for histological grade and main prognostic factors. Methods:195 patients with 44 different diseases have been included. Relatio...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2018/2601281

    authors: Liu DN,Li ZW,Wang HY,Zhao M,Zhao W,Hao CY

    更新日期:2018-07-02 00:00:00

  • Measuring Tumor Metabolism in Pediatric Diffuse Intrinsic Pontine Glioma Using Hyperpolarized Carbon-13 MR Metabolic Imaging.

    abstract:Objective:The purpose of this study was to demonstrate the feasibility of using hyperpolarized carbon-13 (13C) metabolic imaging with [1-13C]-labeled pyruvate for evaluating real-time in vivo metabolism of orthotopic diffuse intrinsic pontine glioma (DIPG) xenografts. Materials and Methods:3D 13C magnetic resonance sp...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2018/3215658

    authors: Autry AW,Hashizume R,James CD,Larson PEZ,Vigneron DB,Park I

    更新日期:2018-07-30 00:00:00

  • Thermodynamic stability and kinetic inertness of a Gd-DTPA bisamide complex grafted onto gold nanoparticles.

    abstract::Gold nanoparticles coated by gadolinium (III) chelates (Au@DTDTPA) where DTDTPA is a dithiolated bisamide derivative of diethylenetriamine-N,N,N',N'',N''-pentaacetic acid (DTPA), constituted contrast agents for both X-ray computed tomography and magnetic resonance imaging. In an MRI context, highly stable Gd(3+) compl...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.1616

    authors: Mogilireddy V,Déchamps-Olivier I,Alric C,Laurent G,Laurent S,Vander Elst L,Muller R,Bazzi R,Roux S,Tillement O,Chuburu F

    更新日期:2015-05-01 00:00:00

  • A First Report on [18F]FPRGD2 PET/CT Imaging in Multiple Myeloma.

    abstract::An observational study was set up to assess the feasibility of [18F]FPRGD2 PET/CT for imaging patients with multiple myeloma (MM) and to compare its detection rate with low dose CT alone and combined [18F]NaF/[18F]FDG PET/CT images. Four patients (2 newly diagnosed patients and 2 with relapsed MM) were included and un...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2017/6162845

    authors: Withofs N,Cousin F,De Prijck B,Bonnet C,Hustinx R,Gambhir SS,Beguin Y,Caers J

    更新日期:2017-07-27 00:00:00

  • An imageable retinoid acid derivative to detect human cancer xenografts and study therapeutic dosing to reduce its toxicity.

    abstract::Developing agents with 'seek, treat and see' capability is critical for personalized molecular medicine. Those agents will specifically target the disease markers for diagnosis and apply the biologically effective dose for treatment. Retinoids regulate a multitude of biological processes. In addition, retinoic acid ca...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.419

    authors: Wang W,Qiu X,Zhang F,Sun J,Cameron AG,Wendt JA,Mawad ME,Ke S

    更新日期:2011-07-01 00:00:00

  • Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma.

    abstract::Glioblastoma multiforme is the most aggressive form of brain tumor. Early and accurate diagnosis of glioma and its borders is an important step for its successful treatment. One of the promising targets for selective visualization of glioma and its margins is connexin 43 (Cx43), which is highly expressed in reactive a...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.1653

    authors: Abakumova T,Abakumov M,Shein S,Chelushkin P,Bychkov D,Mukhin V,Yusubalieva G,Grinenko N,Kabanov A,Nukolova N,Chekhonin V

    更新日期:2016-01-01 00:00:00

  • Assessment of the biodistribution of an [(18) F]FDG-loaded perfluorocarbon double emulsion using dynamic micro-PET in rats.

    abstract::Perfluorocarbon (PFC) double emulsions loaded with a water-soluble, therapeutic agent can be triggered by ultrasound in a process known as acoustic droplet vaporization. Elucidating the stability and biodistribution of these sonosensitive vehicles and encapsulated agents is critical in developing targeted drug deliver...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.1532

    authors: Fabiilli ML,Piert MR,Koeppe RA,Sherman PS,Quesada CA,Kripfgans OD

    更新日期:2013-07-01 00:00:00

  • Quantitative (1)H MRI, (19)F MRI, and (19)F MRS of cell-internalized perfluorocarbon paramagnetic nanoparticles.

    abstract::In vivo molecular imaging with targeted MRI contrast agents will require sensitive methods to quantify local concentrations of contrast agent, enabling not only imaging-based recognition of pathological biomarkers but also detection of changes in expression levels as a consequence of disease development, therapeutic i...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.398

    authors: Kok MB,de Vries A,Abdurrachim D,Prompers JJ,Grüll H,Nicolay K,Strijkers GJ

    更新日期:2011-01-01 00:00:00

  • High-throughput screening of chemical exchange saturation transfer MR contrast agents.

    abstract::A new high-throughput MRI method for screening chemical exchange saturation transfer (CEST) agents is demonstrated, allowing simultaneous testing of multiple samples with minimal attention to sample configuration and shimming of the main magnetic field (B(0)). This approach, which is applicable to diamagnetic, paramag...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.383

    authors: Liu G,Gilad AA,Bulte JW,van Zijl PC,McMahon MT

    更新日期:2010-05-01 00:00:00

  • Improved detection of pancreatic islets in vivo using double contrast.

    abstract::The transplantation of pancreatic islets containing β-cells, which produce insulin, is an alternative approach to the treatment of type 1 diabetes mellitus. The non-invasive visualization of transplanted islets can be performed using MRI; however, this requires labeling of the islets with a suitable contrast agent pri...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.432

    authors: Herynek V,Berková Z,Dovolilová E,Jirák D,Kříž J,Girman P,Saudek F,Hájek M

    更新日期:2011-07-01 00:00:00

  • Clinical and Prognostic Value of PET/CT Imaging with Combination of 68Ga-DOTATATE and 18F-FDG in Gastroenteropancreatic Neuroendocrine Neoplasms.

    abstract:Background:To evaluate the clinical and prognostic value of PET/CT with combination of 68Ga-DOTATATE and 18F-FDG in gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs). Method:83 patients of GEP-NENs who underwent 68Ga-DOTATATE and 18F-FDG PET/CT were enrolled between June 2013 and December 2016. Well-different...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2018/2340389

    authors: Zhang P,Yu J,Li J,Shen L,Li N,Zhu H,Zhai S,Zhang Y,Yang Z,Lu M

    更新日期:2018-02-26 00:00:00

  • Labeling of multiple cell lines using a new iron oxide agent for cell tracking by MRI.

    abstract::Stem cells, cancer cells and immune cells were labeled by co-incubation with a new ultra-small iron oxide nanoparticle called Molday ION Rhodamine-B (MIRB). Iron staining, fluorescence imaging, transmission electron microscopy and flow cytometry were used to assess cell viability, function and labeling efficiency. Thi...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1002/cmmi.456

    authors: McFadden C,Mallett CL,Foster PJ

    更新日期:2011-11-01 00:00:00

  • Hepatic 18F-FDG Uptake Measurements on PET/MR: Impact of Volume of Interest Location on Repeatability.

    abstract:Background:To investigate same day 18F-FDG (Fluorodeoxyglucose) PET (Positron Emission Tomography)/MR (Magnetic Resonance) test-retest repeatability of Standardized Uptake Value measurements normalized for body weight (SUV) and lean body mass (SUL) in different locations in the liver. Methods:This prospective study wa...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章

    doi:10.1155/2017/8639731

    authors: Domachevsky L,Bernstine H,Nidam M,Stein D,Goldberg N,Stern D,Abadi-Korek I,Groshar D

    更新日期:2017-05-30 00:00:00

  • Current Status and Future Perspectives of Artificial Intelligence in Magnetic Resonance Breast Imaging.

    abstract::Recent advances in artificial intelligence (AI) and deep learning (DL) have impacted many scientific fields including biomedical maging. Magnetic resonance imaging (MRI) is a well-established method in breast imaging with several indications including screening, staging, and therapy monitoring. The rapid development a...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章,评审

    doi:10.1155/2020/6805710

    authors: Meyer-Bäse A,Morra L,Meyer-Bäse U,Pinker K

    更新日期:2020-08-28 00:00:00

  • Gadobutrol Precedes Gd-DTPA in Abdominal Contrast-Enhanced MRA and MRI: A Prospective, Multicenter, Intraindividual Study.

    abstract:Objective:To qualitatively and quantitatively compare the contrast-enhanced magnetic resonance angiography (MRA) and magnetic resonance imaging (MRI) in one-stop shop of abdominal imaging with Gadobutrol and Gd-DTPA at equimolar doses of gadolinium. Materials and Methods:This was a prospective designed, multiple cente...

    journal_title:Contrast media & molecular imaging

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1155/2019/9738464

    authors: Liu X,Li Z,Zhang W,Yang C,Diao Y,Duan T,Fu Y,Ren J,Bin S

    更新日期:2019-12-02 00:00:00