Abstract:
:An experimental setup consisting of a hollow fiber module (HFM) was developed for the in vitro study of contrast agent (CA) permeability. Controllable flow and known fiber characteristics allowed permeability studies under well-defined conditions with CAs of different molecular weight (MW). In the MRI experiments performed at 4.7 T, the system was perfused at a constant flow rate (5 ml/min) with water and four CA of different MW: Gd-DOTA (MW=0.6 kDa), P846 (3.5 kDa), P792 (6.5 kDa) and P717 (50.5 kDa). R(1) time courses were measured with a saturation-recovery multi-gradient-echo snapshot sequence in the fiber-free HFM input and the fiber-filled center. Concentration time courses were calculated, and CA extravasation was analyzed with a pharmacokinetic model yielding exchange rate constant k(ie). Only Gd-DOTA (k(ie)=2.37+/-0.16 min(-1)) and P846 (k(ie)=0.58+/-0.17 min(-1)) showed quantifiable extravasation. P717 perfusion yielded an intra-capillary volume fraction of 15.6+/-2.7% compared with 12% estimated from the HFM manufacturer's specifications. In conclusion, the experimental setup allowed classification of in vitro permeability characteristics for CAs with different MW and therefore holds potential for systematic comparison of CAs currently under development.
journal_name
Contrast Media Mol Imagingjournal_title
Contrast media & molecular imagingauthors
Heilmann M,Vautier J,Robert P,Volk Adoi
10.1002/cmmi.262subject
Has Abstractpub_date
2009-03-01 00:00:00pages
66-72issue
2eissn
1555-4309issn
1555-4317journal_volume
4pub_type
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,多中心研究,随机对照试验
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1491
更新日期:2013-01-01 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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更新日期:2018-07-02 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.260
更新日期:2009-01-01 00:00:00
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更新日期:2011-05-01 00:00:00
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journal_title:Contrast media & molecular imaging
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更新日期:2019-04-23 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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更新日期:2011-07-01 00:00:00
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