Abstract:
BACKGROUND:Differentiation of atypical pathogens is important for community-acquired pneumonia (CAP). In this study, we compared sputum and nasopharyngeal swabs (NPS) for use in detection of Mycoplasma pneumoniae (MP), Chlamydophila pneumoniae (CP), and Legionella pneumophila (LP), using Seeplex PneumoBacter ACE Detection Assay (PneumoBacter; Seegene). METHODS:Sputum and NPS specimens were collected from patients in 15 hospitals. DNA was extracted from sputum using QIAamp DNA Stool Mini Kit (Qiagen) and from NPS using easyMAG (bioMérieux). Both types of specimens were evaluated by multiplex PCR using PneumoBacter. To determine the diagnostic performance of this assay, sputum samples were also tested using BD ProbeTec ET Atypical Pneumonia Assay (APA; Becton Dickinson). RESULTS:Among 217 sputum and NPS, 20 (9.2%), 2 (0.9%), and 0 sputum were positive for MP, LP, and CP, respectively, whereas 8 (3.7%) NPS were positive for MP. The sputum APA test yielded 186, 206, and 204 interpretable results for MP, LP, and CP, respectively. Of these, 21 (11.3%) were positive for MP, 2 (1.0%) were positive for LP, and 0 samples were positive for CP. Compared to APA, the sensitivity and specificity of the sputum assay for MP were 95.2% and 100.0%, respectively, whereas for the NPS assay, these were 38.1% and 93.9%. Sputum testing was more sensitive than NPS testing (P=0.002). For LP and CP diagnosis, PneumoBacter and APA tests agreed 100%. CONCLUSIONS:Specimen type is crucial and sputum is preferred over NPS for simultaneous detection of MP, LP, and CP using multiplex PCR in CAP.
journal_name
Ann Lab Medjournal_title
Annals of laboratory medicineauthors
Cho MC,Kim H,An D,Lee M,Noh SA,Kim MN,Chong YP,Woo JHdoi
10.3343/alm.2012.32.2.133subject
Has Abstractpub_date
2012-03-01 00:00:00pages
133-8issue
2eissn
2234-3806issn
2234-3814journal_volume
32pub_type
杂志文章abstract:BACKGROUND:The Automated Fluorescent Immunoassay System (AFIAS) rotavirus assay (Boditech Med Inc., Chuncheon, Korea) is a new rapid antigen test for rotavirus detection. We evaluated the performance of this assay for detecting rotaviruses and their specific genotypes in clinical stool samples. METHODS:AFIAS rotavirus...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2019.39.1.50
更新日期:2019-01-01 00:00:00
abstract::Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood speci...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.2.250
更新日期:2015-03-01 00:00:00
abstract::There have been a number of studies about correlations between HLA genotypes in various ethnic groups and occurrence of various cutaneous adverse drug reactions, ranging in intensity from mild to severe, caused by antiepileptic drugs (AEDs). This is the first report analyzing the HLA genotypes of 9 Korean patients wit...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.5.372
更新日期:2014-09-01 00:00:00
abstract::Metachromatic leukodystrophy (MLD) is an autosomal recessive disease caused by a deficiency in arylsulfatase A (ARSA). However, decreased ARSA activity is also observed in pseudodeficiency (PD). To distinguish between MLD and PD, we performed gene mutation and sulfatide analyses by using dried blood spots (DBSs) from ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.4.458
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and its specific tissue inhibitor - tissue inhibitor of metalloproteinases-1 (TIMP-1) may play an important role in the pathogenesis and spread of cancer. We investigated the plasma levels of M-CSF, MMP-9, and TIMP-1 in compari...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.3.223
更新日期:2016-05-01 00:00:00
abstract::Guidelines recommend that clinical laboratories perform phenotypic tests (nitrocefin-based test and penicillin 10-U [P10] or 1-U [P1] zone edge tests) to detect penicillinase in Staphylococcus aureus isolates. This study aimed to assess the prevalence of blaZ encoding penicillinase and perform various phenotypic tests...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.2.155
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:In the past, ABO incompatibility was an absolute contraindication for solid organ transplantation. However, multiple recent trials have suggested strategies for overcoming the reactions between graft antigens and recipient antibodies that cause graft rejection. In this study, we determined the usefulness of ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2012.32.4.283
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (cari...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2019.39.2.176
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:The storage temperature and time of blood gas samples collected in syringes constitute preanalytical variables that could affect blood gas or lactate concentration measurement results. We analyzed the effect of storage temperature and time delay on arterial or venous blood gas stability related to pH, partia...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.6.448
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.2.142
更新日期:2020-03-01 00:00:00
abstract::The platelet-to-lymphocyte ratio (PLR) has emerged as an informative marker revealing shifts in platelet and lymphocyte counts due to acute inflammatory and prothrombotic states. PLR has been extensively examined in neoplastic diseases accompanied by immune suppression and thrombosis, which can be predicted by combine...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
doi:10.3343/alm.2019.39.4.345
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.4.306
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST an...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.2.194
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:With the advent of sodium glucose co-transporter 2 inhibitors to control glucose and treat diabetes, laboratory data aided by either timed or spot glucose levels in the urine could be used as an alternative marker of drug response. The aim of this study was to assess the agreement between overnight urinary g...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.1.9
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequen...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.6.618
更新日期:2015-11-01 00:00:00
abstract::Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.4.312
更新日期:2020-07-01 00:00:00
abstract::ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2013.33.3.208
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:We reviewed patients with multiple myeloma (MM) in order to assess the incidence of genetic abnormalities and their associations with clinical parameters, risk groups, and prognosis. METHODS:A total of 130 patients with MM were enrolled. The incidences of genetic abnormalities were determined in all patient...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2013.33.4.248
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.4.313
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:The impact of HLA and KIR ligand mismatching on the outcome of hematopoietic stem cell transplantation (HSCT) remains unclear. Previous reports have identified considerable ethnic differences in the impact of HLA and KIR ligand mismatches, as well as KIR ligand status, on HSCT; however, to date, no data has ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.1.111
更新日期:2015-01-01 00:00:00
abstract::Long QT syndrome (LQTS) is an inherited cardiac disease characterized by a prolonged heart rate-corrected QT (QTc) interval. We investigated the genetic causes in patients with prolonged QTc intervals who were negative for pathogenic variants in three major LQTS-related genes (KCNQ1, KCNH2, and SCN5A). Molecular genet...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.1.54
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood cult...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.6.446
更新日期:2014-11-01 00:00:00
abstract::As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven MRSA...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.1.57
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA). METHODS:A total of 530 fresh stool specimens were collec...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.1.15
更新日期:2014-01-01 00:00:00
abstract::Candidemia due to uncommon Candida spp. appears to be increasing in incidence. C. dubliniensis has been increasingly recovered from individuals not infected with HIV. Identification of C. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to C. albicans. We report the first ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2012.32.3.225
更新日期:2012-05-01 00:00:00
abstract::The 2016 WHO diagnostic criteria for chronic myelomonocytic leukemia (CMML) require both absolute and relative monocytosis (≥1×10⁹/L and ≥10% of white blood cell counts) in peripheral blood. Moreover, myeloproliferative neoplasm (MPN) features in bone marrow and/or MPN-associated mutations tend to support MPN with mon...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.5.481
更新日期:2018-09-01 00:00:00
abstract:Background:Plasma cell myeloma (PCM) is caused by immune dysregulation. We evaluated the expression of immune checkpoint programmed cell death protein-1 (PD-1) on T cell subsets in PCM patients according to disease course and cytogenetic abnormalities. This study aimed to find a target group suitable for therapeutic us...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2021.41.3.259
更新日期:2021-05-01 00:00:00
abstract:BACKGROUND:Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.4.291
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.1.50
更新日期:2015-01-01 00:00:00
abstract::Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 20...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.1.55
更新日期:2016-01-01 00:00:00