Abstract:
BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood culture samples from patients with a delta neutrophil index greater than 2.7% were selected from March to July in 2013. Three hundred positive and 1,100 negative for bacterial growth in blood culture bottles samples were tested by conventional and real-time PCR-REBA, respectively. RESULTS:The overall agreement between the conventional identification test and the REBA Sepsis-ID test was 95.3% (286/300). Agreement for gram-positive bacteria, gram-negative bacteria, fungi, and polymicrobials was 94.5% (190/201), 97.3% (71/73), 100% (14/14), and 91.7% (11/12), respectively. The detection rate of the mecA gene from methicillin-resistant Staphylococcus isolates was 97.8% (90/92). The vanA gene was detected in one blood culture sample from which vancomycin-resistant Enterococcus was isolated. When the cycle threshold for real-time PCR was defined as 30.0, 2.4% (26/1,100) of negative blood culture samples tested positive by real-time PCR. CONCLUSIONS:The REBA Sepsis-ID test is capable of simultaneously and quickly detecting both causative agents and antimicrobial resistance genes, such as mecA and van, in blood culture positive samples.
journal_name
Ann Lab Medjournal_title
Annals of laboratory medicineauthors
Park SD,Lee G,Wang HY,Park M,Kim S,Kim H,Kim J,Kim YK,Kim HY,Lee H,Uh Y,Kim JBdoi
10.3343/alm.2014.34.6.446subject
Has Abstractpub_date
2014-11-01 00:00:00pages
446-55issue
6eissn
2234-3806issn
2234-3814journal_volume
34pub_type
杂志文章abstract::Guidelines recommend that clinical laboratories perform phenotypic tests (nitrocefin-based test and penicillin 10-U [P10] or 1-U [P1] zone edge tests) to detect penicillinase in Staphylococcus aureus isolates. This study aimed to assess the prevalence of blaZ encoding penicillinase and perform various phenotypic tests...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.2.155
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reactions. Therefore, a method to measure antibodies, including IgG, is necessary. We investigated ABO antibody titers of healthy individuals using a column ag...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.3.223
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequen...
journal_title:Annals of laboratory medicine
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更新日期:2015-11-01 00:00:00
abstract::Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood speci...
journal_title:Annals of laboratory medicine
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abstract:BACKGROUND:Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread...
journal_title:Annals of laboratory medicine
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abstract::ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...
journal_title:Annals of laboratory medicine
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abstract:BACKGROUND:Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (cari...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a rare inherited disorder characterized by infantile-onset macrocephaly, slow neurologic deterioration, and seizures. Mutations in the causative gene, MLC1, are found in approximately 75% of patients and are inherited in an autosomal recessi...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.6.516
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:Clopidogrel is one of the most commonly used anti-platelet agents in cardiovascular diseases. We analyzed the relationship between the platelet function analyzer (PFA)-200 P2Y (INNOVANCE PFA-200 System, Siemens Healthcare, Germany) results and occurrence of major adverse cardiac events (MACEs) in Korean pati...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.5.413
更新日期:2018-09-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2019.39.3.284
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.1.50
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.3.240
更新日期:2017-05-01 00:00:00
abstract::Procalcitonin (PCT) is used as a biomarker for the diagnosis of sepsis, severe sepsis and septic shock. At the same time, PCT has also been used to guide antibiotic therapy. This review outlines the main indications for PCT measurement and points out possible pitfalls. The classic indications for PCT measurement are: ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
doi:10.3343/alm.2014.34.4.263
更新日期:2014-07-01 00:00:00
abstract::Changes in microparticles (MP) from red blood cell (RBC) concentrates in the context of irradiation have not been investigated. The aim of this study was to evaluate how irradiation affects the number of MPs within transfusion components. Twenty RBC concentrates, within 14 days after donation, were exposed to gamma ra...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.4.362
更新日期:2016-07-01 00:00:00
abstract::Metachromatic leukodystrophy (MLD) is an autosomal recessive disease caused by a deficiency in arylsulfatase A (ARSA). However, decreased ARSA activity is also observed in pseudodeficiency (PD). To distinguish between MLD and PD, we performed gene mutation and sulfatide analyses by using dried blood spots (DBSs) from ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.4.458
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia with a vancomycin minimum inhibitory concentration (MIC) of 2 µg/mL presents a high rate of therapeutic failure in response to vancomycin. In addition, polymorphism in accessory gene regulator (agr) is associated with vancomycin therapeutic effect...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.4.399
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.4.291
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:The clindamycin-resistant erythromycin-susceptible (CRES) phenotype is rare in Streptococcus agalactiae (group B streptococci). We aimed to determine the molecular characteristics of CRES S. agalactiae using whole genome sequencing (WGS). METHODS:Sixty-six S. agalactiae isolates obtained from blood (N=26), ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.5.370
更新日期:2020-09-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.2.137
更新日期:2017-03-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.5.450
更新日期:2016-09-01 00:00:00
abstract::[This corrects the article on p. 439 in vol. 40.]. ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章,已发布勘误
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更新日期:2021-01-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.3.321
更新日期:2015-05-01 00:00:00
abstract::Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 20...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.1.55
更新日期:2016-01-01 00:00:00
abstract::Myocardial infarction (MI) is the leading cause of death in the developed world. Biomarkers have an essential role in diagnosis, risk stratification, guiding management and clinical decision making in the setting of patients presenting with signs and symptoms of MI. Cardiac troponin (cTn) rose to prominence during the...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
doi:10.3343/alm.2013.33.5.309
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND:We evaluated the performance of four commercial nucleic acid amplification tests (NAATs: Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile) for direct and rapid detection of Clostridium difficile toxin genes. METHODS:We compared four NAATs on the same set of 3...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.2.131
更新日期:2016-03-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.5.523
更新日期:2015-09-01 00:00:00
abstract::We aimed to observe antimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections. Two hundred sixty-eight E. coli strains were obtained from outpatients with various infections at different polyclinics at the 82nd Year of State Hospital in Rize, Turkey. Sus...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.2.139
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST an...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.2.194
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:With the advent of sodium glucose co-transporter 2 inhibitors to control glucose and treat diabetes, laboratory data aided by either timed or spot glucose levels in the urine could be used as an alternative marker of drug response. The aim of this study was to assess the agreement between overnight urinary g...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.1.9
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Clarithromycin, amoxicillin, metronidazole, tetracycline, and levofloxacin have been commonly used for the eradication of Helicobacter pylori. We compared the change in antibiotic resistance of H. pylori strains during two separate periods and investigated the effect of antibiotic resistance on H. pylori era...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00