Comparison of red blood cell hemolysis using plasma and serum separation tubes for outpatient specimens.

Abstract:

BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST and SST and applied this for choosing appropriate tubes for clinical laboratories. METHODS:The HI of specimens obtained from outpatients visiting Asan Medical Center between July and December 2012 was analyzed. The HI was scored from 0 to 10 by using the Toshiba 200FR (Toshiba Medical Systems Co., Japan). HI was classified by sample tube type, and significant hemolysis was defined as a HI of 2 or more. For significant hemolysis cases, medical records were reviewed to identify the causes. RESULTS:Among 171,519 specimens, significant hemolysis was observed in 0.66% of specimens (0.68% of PST specimens, 0.46% of SST specimens). The mean HI in PST was 0.18 (SD: 0.43) and that in SST was 0.14 (SD: 0.37). The proportion of significant hemolysis was significantly higher in PST than in SST (P=0.001). The cause of significant hemolysis was identified as chemotherapy and prosthetic valve in 48.1% of specimens. Complex sampling errors may have caused significant hemolysis in the remaining 51.9% of specimens. CONCLUSIONS:The incidence of hemolysis was slightly higher for PST than SST, although both were <1%. PST are thought to be more useful than SST in outpatient testing because of rapid turnaround time, greater sample volume, and less risk of random errors due to fibrin strands.

journal_name

Ann Lab Med

authors

Ko DH,Won D,Jeong TD,Lee W,Chun S,Min WK

doi

10.3343/alm.2015.35.2.194

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

194-7

issue

2

eissn

2234-3806

issn

2234-3814

journal_volume

35

pub_type

杂志文章
  • The impact of HLA and KIR ligand mismatching on unrelated allogeneic hematopoietic stem cell transplantation in Korean adult patients.

    abstract:BACKGROUND:The impact of HLA and KIR ligand mismatching on the outcome of hematopoietic stem cell transplantation (HSCT) remains unclear. Previous reports have identified considerable ethnic differences in the impact of HLA and KIR ligand mismatches, as well as KIR ligand status, on HSCT; however, to date, no data has ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.1.111

    authors: Park H,Rho EY,In JW,Kim I,Yoon SS,Park S,Shin S,Park KU,Song EY

    更新日期:2015-01-01 00:00:00

  • Role of plasma exchange in ABO-incompatible kidney transplantation.

    abstract:BACKGROUND:In the past, ABO incompatibility was an absolute contraindication for solid organ transplantation. However, multiple recent trials have suggested strategies for overcoming the reactions between graft antigens and recipient antibodies that cause graft rejection. In this study, we determined the usefulness of ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.4.283

    authors: Yoo S,Lee EY,Huh KH,Kim MS,Kim YS,Kim HO

    更新日期:2012-07-01 00:00:00

  • Plasma Levels and Diagnostic Utility of Macrophage Colony-Stimulating Factor, Matrix Metalloproteinase-9, and Tissue Inhibitor of Metalloproteinases-1 as New Biomarkers of Breast Cancer.

    abstract:BACKGROUND:Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and its specific tissue inhibitor - tissue inhibitor of metalloproteinases-1 (TIMP-1) may play an important role in the pathogenesis and spread of cancer. We investigated the plasma levels of M-CSF, MMP-9, and TIMP-1 in compari...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2016.36.3.223

    authors: Ławicki S,Głażewska EK,Sobolewska M,Będkowska GE,Szmitkowski M

    更新日期:2016-05-01 00:00:00

  • Characterization of Extended-Spectrum β-Lactamase Genes of Shigella flexneri Isolates With Fosfomycin Resistance From Patients in China.

    abstract:BACKGROUND:The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flex...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2017.37.5.415

    authors: Liu Y,Cheng Y,Yang H,Hu L,Cheng J,Ye Y,Li J

    更新日期:2017-09-01 00:00:00

  • Establishing reference intervals for sex hormones on the analytical platforms Advia Centaur and Immulite 2000XP.

    abstract::Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 20...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2016.36.1.55

    authors: Schüring AN,Kelsch R,Pierściński G,Nofer JR

    更新日期:2016-01-01 00:00:00

  • Clonal spread of carbapenem non-susceptible Acinetobacter baumannii in an intensive care unit in a teaching hospital in China.

    abstract:BACKGROUND:This study was aimed to investigate the genetic diversity and antibiotic resistance profile of the nosocomial infection agent Acinetobacter baumannii from a medical intensive care unit (ICU) in a teaching hospital in Suzhou, China. METHODS:The genetic relationship among A. baumannii isolates in an ICU was i...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.6.413

    authors: Zhong Q,Xu W,Wu Y,Xu H

    更新日期:2012-11-01 00:00:00

  • Accessory Gene Regulator Polymorphism and Vancomycin Minimum Inhibitory Concentration in Methicillin-Resistant Staphylococcus aureus.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia with a vancomycin minimum inhibitory concentration (MIC) of 2 µg/mL presents a high rate of therapeutic failure in response to vancomycin. In addition, polymorphism in accessory gene regulator (agr) is associated with vancomycin therapeutic effect...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.4.399

    authors: Park MJ,Kim HS,Kim HS,Kim JS,Song W,Kim MY,Lee YK,Kang HJ

    更新日期:2015-07-01 00:00:00

  • Application of Multigene Panel Sequencing in Patients with Prolonged Rate-corrected QT Interval and No Pathogenic Variants Detected in KCNQ1, KCNH2, and SCN5A.

    abstract::Long QT syndrome (LQTS) is an inherited cardiac disease characterized by a prolonged heart rate-corrected QT (QTc) interval. We investigated the genetic causes in patients with prolonged QTc intervals who were negative for pathogenic variants in three major LQTS-related genes (KCNQ1, KCNH2, and SCN5A). Molecular genet...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2018.38.1.54

    authors: Seo SH,Kim SY,Cho SI,Park H,Lee S,Choi JM,Kim MJ,Lee JS,Ahn KJ,Song MK,Bae EJ,Park SS,Seong MW

    更新日期:2018-01-01 00:00:00

  • Linezolid Resistance in Methicillin-Resistant Staphylococcus aureus in Korea: High Rate of False Resistance to Linezolid by the VITEK 2 System.

    abstract::As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven MRSA...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2020.40.1.57

    authors: Yoo IY,Kang OK,Shim HJ,Huh HJ,Lee NY

    更新日期:2020-01-01 00:00:00

  • Clinical and microbiological characteristics of six Staphylococcus pettenkoferi isolates from blood samples.

    abstract::Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood speci...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.2.250

    authors: Park S,Chung HS,Lee M

    更新日期:2015-03-01 00:00:00

  • Point of care D-dimer testing in the emergency department: a bioequivalence study.

    abstract:BACKGROUND:D-dimer is used widely as a diagnostic aid in low- and moderate-risk patients with suspected venous thromboembolism (VTE). While our laboratory utilizes VIDAS D-dimer analyzer (bioMérieux SA, France), our emergency department (ED) recently procured a D-dimer analyzer AQT90 FLEX (Radiometer Medical ApS, Denma...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2013.33.1.34

    authors: Perveen S,Unwin D,Shetty AL

    更新日期:2013-01-01 00:00:00

  • Evaluation of PCR-reverse blot hybridization assay, REBA Sepsis-ID test, for simultaneous identification of bacterial pathogens and mecA and van genes from blood culture bottles.

    abstract:BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood cult...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2014.34.6.446

    authors: Park SD,Lee G,Wang HY,Park M,Kim S,Kim H,Kim J,Kim YK,Kim HY,Lee H,Uh Y,Kim JB

    更新日期:2014-11-01 00:00:00

  • Comparison of total and IgG ABO antibody titers in healthy individuals by using tube and column agglutination techniques.

    abstract:BACKGROUND:Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reactions. Therefore, a method to measure antibodies, including IgG, is necessary. We investigated ABO antibody titers of healthy individuals using a column ag...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2014.34.3.223

    authors: Park ES,Jo KI,Shin JW,Park R,Choi TY,Bang HI,Chai GR,Yun SG

    更新日期:2014-05-01 00:00:00

  • Impact of genetic abnormalities on the prognoses and clinical parameters of patients with multiple myeloma.

    abstract:BACKGROUND:We reviewed patients with multiple myeloma (MM) in order to assess the incidence of genetic abnormalities and their associations with clinical parameters, risk groups, and prognosis. METHODS:A total of 130 patients with MM were enrolled. The incidences of genetic abnormalities were determined in all patient...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2013.33.4.248

    authors: Jekarl DW,Min CK,Kwon A,Kim H,Chae H,Kim M,Lim J,Kim Y,Han K

    更新日期:2013-07-01 00:00:00

  • Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana benthamiana.

    abstract:BACKGROUND:Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2017.37.4.313

    authors: Mazalovska M,Varadinov N,Koynarski T,Minkov I,Teoharov P,Lomonossoff GP,Zahmanova G

    更新日期:2017-07-01 00:00:00

  • Single-color multitarget flow cytometry using monoclonal antibodies labeled with different intensities of the same fluorochrome.

    abstract:BACKGROUND:We developed a single-color multitarget flow cytometry (SM-FC) assay, a single-tube assay with graded mean fluorescence intensities (MFIs). We evaluated the repeatability of SM-FC, and its correlation with multicolor flow cytometry (MFC), to assess its application as a routine FC assay. METHODS:We selected ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.3.171

    authors: Park J,Han K

    更新日期:2012-05-01 00:00:00

  • Absence of HLA-B*1502 and HLA-A*3101 alleles in 9 Korean patients with antiepileptic drug-induced skin rash: a preliminary study.

    abstract::There have been a number of studies about correlations between HLA genotypes in various ethnic groups and occurrence of various cutaneous adverse drug reactions, ranging in intensity from mild to severe, caused by antiepileptic drugs (AEDs). This is the first report analyzing the HLA genotypes of 9 Korean patients wit...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2014.34.5.372

    authors: Song JS,Kang ES,Joo EY,Hong SB,Seo DW,Lee SY

    更新日期:2014-09-01 00:00:00

  • Evaluation of Xpert Carba-R Assay v.2 to Detect Carbapenemase Genes in Two Hospitals in Korea.

    abstract:BACKGROUND:As the spread of carbapenemase-producing Enterobacteriaceae poses a critical threat to public health, rapid detection of carbapenemase genes is urgently required for prompt initiation of appropriate antimicrobial therapy and infection control. We evaluated the performance of Xpert Carba-R v.2 (Cepheid, USA) ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2020.40.3.209

    authors: Byun JH,Kim YA,Kim M,Kim B,Choi JY,Park YS,Yong D

    更新日期:2020-05-01 00:00:00

  • Immune Checkpoint Programmed Cell Death Protein-1 (PD-1) Expression on Bone Marrow T Cell Subsets in Patients With Plasma Cell Myeloma.

    abstract:Background:Plasma cell myeloma (PCM) is caused by immune dysregulation. We evaluated the expression of immune checkpoint programmed cell death protein-1 (PD-1) on T cell subsets in PCM patients according to disease course and cytogenetic abnormalities. This study aimed to find a target group suitable for therapeutic us...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2021.41.3.259

    authors: Lee MY,Park CJ,Cho YU,You E,Jang S,Seo EJ,Lee JH,Yoon DH,Suh C

    更新日期:2021-05-01 00:00:00

  • Effectiveness of Plasma and Urine Neutrophil Gelatinase-Associated Lipocalin for Predicting Acute Kidney Injury in High-Risk Patients.

    abstract:BACKGROUND:Neutrophil gelatinase-associated lipocalin (NGAL) is a useful biomarker for acute kidney injury (AKI) prediction. However, studies on whether using both plasma NGAL (PNGAL) and urine NGAL (UNGAL) can improve AKI prediction are limited. We investigated the best approach to predict AKI in high-risk patients wh...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2021.41.1.60

    authors: Yi A,Lee CH,Yun YM,Kim H,Moon HW,Hur M

    更新日期:2021-01-01 00:00:00

  • Highly sensitive and novel point-of-care system, aQcare Chlamydia TRF kit for detecting Chlamydia trachomatis by using europium (Eu) (III) chelated nanoparticles.

    abstract:BACKGROUND:The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.1.50

    authors: Ham JY,Jung J,Hwang BG,Kim WJ,Kim YS,Kim EJ,Cho MY,Hwang MS,Won DI,Suh JS

    更新日期:2015-01-01 00:00:00

  • Update on procalcitonin measurements.

    abstract::Procalcitonin (PCT) is used as a biomarker for the diagnosis of sepsis, severe sepsis and septic shock. At the same time, PCT has also been used to guide antibiotic therapy. This review outlines the main indications for PCT measurement and points out possible pitfalls. The classic indications for PCT measurement are: ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.3343/alm.2014.34.4.263

    authors: Meisner M

    更新日期:2014-07-01 00:00:00

  • New decision criteria for selecting delta check methods based on the ratio of the delta difference to the width of the reference range can be generally applicable for each clinical chemistry test item.

    abstract:BACKGROUND:Many laboratories use 4 delta check methods: delta difference, delta percent change, rate difference, and rate percent change. However, guidelines regarding decision criteria for selecting delta check methods have not yet been provided. We present new decision criteria for selecting delta check methods for e...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.5.345

    authors: Park SH,Kim SY,Lee W,Chun S,Min WK

    更新日期:2012-09-01 00:00:00

  • Galectin-3 Reflects the Echocardiographic Grades of Left Ventricular Diastolic Dysfunction.

    abstract:BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2018.38.4.306

    authors: Ansari U,Behnes M,Hoffmann J,Natale M,Fastner C,El-Battrawy I,Rusnak J,Kim SH,Lang S,Hoffmann U,Bertsch T,Borggrefe M,Akin I

    更新日期:2018-07-01 00:00:00

  • The Platelet-to-Lymphocyte Ratio as an Inflammatory Marker in Rheumatic Diseases.

    abstract::The platelet-to-lymphocyte ratio (PLR) has emerged as an informative marker revealing shifts in platelet and lymphocyte counts due to acute inflammatory and prothrombotic states. PLR has been extensively examined in neoplastic diseases accompanied by immune suppression and thrombosis, which can be predicted by combine...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.3343/alm.2019.39.4.345

    authors: Gasparyan AY,Ayvazyan L,Mukanova U,Yessirkepov M,Kitas GD

    更新日期:2019-07-01 00:00:00

  • Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.

    abstract:BACKGROUND:ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA). METHODS:A total of 530 fresh stool specimens were collec...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2014.34.1.15

    authors: Shin BM,Lee EJ

    更新日期:2014-01-01 00:00:00

  • Erratum: COVID-19 Molecular Testing in Korea: Practical Essentials and Answers From Experts Based on Experiences of Emergency Use Authorization Assays.

    abstract::[This corrects the article on p. 439 in vol. 40.]. ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章,已发布勘误

    doi:10.3343/alm.2021.41.1.126

    authors: Sung H,Roh KH,Hong KH,Seong MW,Ryoo N,Kim HS,Lee J,Kim SY,Ryu SW,Kim MN,Han MG,Lee SW,Lee H,Yoo CK

    更新日期:2021-01-01 00:00:00

  • Duffy blood group genotyping in Thai blood donors.

    abstract:BACKGROUND:Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequen...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.6.618

    authors: Nathalang O,Intharanut K,Siriphanthong K,Nathalang S,Kupatawintu P

    更新日期:2015-11-01 00:00:00

  • Prediction of HLA-DQ in Deceased Donors and its Clinical Significance in Kidney Transplantation.

    abstract:Background:HLA-DQ typing in deceased donors is not mandatory in Korea. Therefore, when patients develop DQ antibodies after kidney transplantation (KT) from deceased donor, it is impossible to determine whether they are donor-specific antibodies (DSA). We developed DQ prediction programs for the HLA gene and evaluated ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2021.41.2.190

    authors: Kim SK,Yang JJ,Hwang SH,Sung H,Shin S,Ko SY,Oh HB

    更新日期:2021-03-01 00:00:00

  • Budget Impact of the Accreditation Program for Clinical Laboratories on Colorectal Cancer Screening via Fecal Immunochemical Testing: Results from the National Cancer Screening Program in Korea.

    abstract:BACKGROUND:False-positive fecal immunochemical test (FIT) results in the National Cancer Screening Program (NCSP) for colorectal cancer may lead to unnecessary procedures, such as colonoscopies, increasing the medical costs. We estimated reductions in the cost of National Health Insurance according to the accreditation...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2018.38.3.249

    authors: Jun JK,Sung NY,Song SH,Hong S,Jang MA,Song J,Kim JH,Min WK,Lee YK

    更新日期:2018-05-01 00:00:00