Development of an Automated Image Analyzer for Microvessel Density Measurement in Bone Marrow Biopsies.

abstract：BACKGROUND:Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (cari...

journal_title：Annals of laboratory medicine

authors： Huh HJ,Lim KR,Ki CS,Huh K,Shim HJ,Song DJ,Kim YJ,Chung DR,Lee NY

更新日期：2019-03-01 00:00:00

abstract：BACKGROUND:The storage temperature and time of blood gas samples collected in syringes constitute preanalytical variables that could affect blood gas or lactate concentration measurement results. We analyzed the effect of storage temperature and time delay on arterial or venous blood gas stability related to pH, partia...

journal_title：Annals of laboratory medicine

authors： Arbiol-Roca A,Imperiali CE,Dot-Bach D,Valero-Politi J,Dastis-Arias M

更新日期：2020-11-01 00:00:00

abstract：BACKGROUND:During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in Korea. We performed analytical and clinical validations of these kits. METH...

journal_title：Annals of laboratory medicine

authors： Kim MN,Ko YJ,Seong MW,Kim JS,Shin BM,Sung H

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:We reviewed patients with multiple myeloma (MM) in order to assess the incidence of genetic abnormalities and their associations with clinical parameters, risk groups, and prognosis. METHODS:A total of 130 patients with MM were enrolled. The incidences of genetic abnormalities were determined in all patient...

journal_title：Annals of laboratory medicine

authors： Jekarl DW,Min CK,Kwon A,Kim H,Chae H,Kim M,Lim J,Kim Y,Han K

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND:Clarithromycin, amoxicillin, metronidazole, tetracycline, and levofloxacin have been commonly used for the eradication of Helicobacter pylori. We compared the change in antibiotic resistance of H. pylori strains during two separate periods and investigated the effect of antibiotic resistance on H. pylori era...

journal_title：Annals of laboratory medicine

authors： An B,Moon BS,Kim H,Lim HC,Lee YC,Lee G,Kim SH,Park M,Kim JB

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:Delamanid, bedaquiline, and linezolid have recently been approved for the treatment of multidrug- and extensively drug-resistant (MDR and XDR, respectively) tuberculosis (TB). To use these drugs effectively, drug susceptibility tests, including rapid molecular techniques, are required for accurate diagnosis ...

journal_title：Annals of laboratory medicine

authors： Yang JS,Kim KJ,Choi H,Lee SH

更新日期：2018-11-01 00:00:00

abstract：BACKGROUND:Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. ...

journal_title：Annals of laboratory medicine

authors： Chang Y,Kim S,Kim Y,Ei PW,Hwang D,Lee J,Chang CL,Lee H

更新日期：2020-03-01 00:00:00

abstract：BACKGROUND:False-positive fecal immunochemical test (FIT) results in the National Cancer Screening Program (NCSP) for colorectal cancer may lead to unnecessary procedures, such as colonoscopies, increasing the medical costs. We estimated reductions in the cost of National Health Insurance according to the accreditation...

journal_title：Annals of laboratory medicine

authors： Jun JK,Sung NY,Song SH,Hong S,Jang MA,Song J,Kim JH,Min WK,Lee YK

更新日期：2018-05-01 00:00:00

abstract：BACKGROUND:We evaluated the performance of four commercial nucleic acid amplification tests (NAATs: Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile) for direct and rapid detection of Clostridium difficile toxin genes. METHODS:We compared four NAATs on the same set of 3...

journal_title：Annals of laboratory medicine

authors： Shin BM,Yoo SM,Shin WC

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND:Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and its specific tissue inhibitor - tissue inhibitor of metalloproteinases-1 (TIMP-1) may play an important role in the pathogenesis and spread of cancer. We investigated the plasma levels of M-CSF, MMP-9, and TIMP-1 in compari...

journal_title：Annals of laboratory medicine

authors： Ławicki S,Głażewska EK,Sobolewska M,Będkowska GE,Szmitkowski M

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:Although Th2 immune activation is predominant in allergic diseases, neopterinlevels and indoleamine 2,3-dioxygenase (IDO)-1 activity (kynurenine:tryptophan ratio), which reflect Th1 immune activity, increase with interferon-gamma (IFN-γ) stimulation. We investigated neopterin, tryptophan, and kynurenine leve...

journal_title：Annals of laboratory medicine

authors： Ünüvar S,Erge D,Kılıçarslan B,Gözükara Bağ HG,Çatal F,Girgin G,Baydar T

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia with a vancomycin minimum inhibitory concentration (MIC) of 2 µg/mL presents a high rate of therapeutic failure in response to vancomycin. In addition, polymorphism in accessory gene regulator (agr) is associated with vancomycin therapeutic effect...

journal_title：Annals of laboratory medicine

authors： Park MJ,Kim HS,Kim HS,Kim JS,Song W,Kim MY,Lee YK,Kang HJ

更新日期：2015-07-01 00:00:00

abstract：:[This corrects the article on p. 439 in vol. 40.]. ...

journal_title：Annals of laboratory medicine

authors： Sung H,Roh KH,Hong KH,Seong MW,Ryoo N,Kim HS,Lee J,Kim SY,Ryu SW,Kim MN,Han MG,Lee SW,Lee H,Yoo CK

更新日期：2021-01-01 00:00:00

abstract：:Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood speci...

journal_title：Annals of laboratory medicine

authors： Park S,Chung HS,Lee M

更新日期：2015-03-01 00:00:00

abstract：:As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven MRSA...

journal_title：Annals of laboratory medicine

authors： Yoo IY,Kang OK,Shim HJ,Huh HJ,Lee NY

更新日期：2020-01-01 00:00:00

abstract：:ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...

journal_title：Annals of laboratory medicine

authors： Jung CL,Cha MK,Jun BH,Hong KS

更新日期：2013-05-01 00:00:00

abstract：:The 2016 WHO diagnostic criteria for chronic myelomonocytic leukemia (CMML) require both absolute and relative monocytosis (≥1×10⁹/L and ≥10% of white blood cell counts) in peripheral blood. Moreover, myeloproliferative neoplasm (MPN) features in bone marrow and/or MPN-associated mutations tend to support MPN with mon...

journal_title：Annals of laboratory medicine

authors： Moon Y,Kim MH,Kim HR,Ahn JY,Huh J,Huh JY,Han JH,Park JS,Cho SR

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flex...

journal_title：Annals of laboratory medicine

authors： Liu Y,Cheng Y,Yang H,Hu L,Cheng J,Ye Y,Li J

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND:Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequ...

journal_title：Annals of laboratory medicine

authors： Jeong JH,Lee HT,Seo JY,Seo YH,Kim KH,Kim MJ,Lee JH,Park J,Hong JS,Park PW,Ahn JY

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing...

journal_title：Annals of laboratory medicine

authors： Yoo IY,Oh JW,Cha HS,Koh EM,Kang ES

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood cult...

journal_title：Annals of laboratory medicine

authors： Park SD,Lee G,Wang HY,Park M,Kim S,Kim H,Kim J,Kim YK,Kim HY,Lee H,Uh Y,Kim JB

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:A newborn screening (NBS) program has been utilized to detect asymptomatic newborns with inherited metabolic diseases (IMDs). There have been some bottlenecks such as false-positives and imprecision in the current NBS tests. To overcome these issues, we developed a multigene panel for IMD testing and investi...

journal_title：Annals of laboratory medicine

authors： Park KJ,Park S,Lee E,Park JH,Park JH,Park HD,Lee SY,Kim JW

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:In the past, ABO incompatibility was an absolute contraindication for solid organ transplantation. However, multiple recent trials have suggested strategies for overcoming the reactions between graft antigens and recipient antibodies that cause graft rejection. In this study, we determined the usefulness of ...

journal_title：Annals of laboratory medicine

authors： Yoo S,Lee EY,Huh KH,Kim MS,Kim YS,Kim HO

更新日期：2012-07-01 00:00:00

abstract：BACKGROUND:D-dimer is used widely as a diagnostic aid in low- and moderate-risk patients with suspected venous thromboembolism (VTE). While our laboratory utilizes VIDAS D-dimer analyzer (bioMérieux SA, France), our emergency department (ED) recently procured a D-dimer analyzer AQT90 FLEX (Radiometer Medical ApS, Denma...

journal_title：Annals of laboratory medicine

authors： Perveen S,Unwin D,Shetty AL

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:With the advent of sodium glucose co-transporter 2 inhibitors to control glucose and treat diabetes, laboratory data aided by either timed or spot glucose levels in the urine could be used as an alternative marker of drug response. The aim of this study was to assess the agreement between overnight urinary g...

journal_title：Annals of laboratory medicine

authors： Kim SR,Lee YH,Lee SG,Lee SH,Kang ES,Cha BS,Lee HC,Kim JH,Lee BW

更新日期：2017-01-01 00:00:00

abstract：:Guidelines recommend that clinical laboratories perform phenotypic tests (nitrocefin-based test and penicillin 10-U [P10] or 1-U [P1] zone edge tests) to detect penicillinase in Staphylococcus aureus isolates. This study aimed to assess the prevalence of blaZ encoding penicillinase and perform various phenotypic tests...

journal_title：Annals of laboratory medicine

authors： Takayama Y,Tanaka T,Oikawa K,Fukano N,Goto M,Takahashi T

更新日期：2018-03-01 00:00:00

abstract：BACKGROUND:Narcolepsy is a neurologic disorder characterized by excessive daytime sleepiness, symptoms of abnormal rapid eye movement (REM) sleep, and a strong association with HLA-DRB1*1501, -DQA1*0102, and -DQB1*0602. Here, we investigated the clinico-physical characteristics of Korean patients with narcolepsy, their...

journal_title：Annals of laboratory medicine

authors： Woo HI,Joo EY,Hong SB,Lee KW,Kang ES

更新日期：2012-01-01 00:00:00

abstract：BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST an...

journal_title：Annals of laboratory medicine

authors： Ko DH,Won D,Jeong TD,Lee W,Chun S,Min WK

更新日期：2015-03-01 00:00:00

abstract：BACKGROUND:ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA). METHODS:A total of 530 fresh stool specimens were collec...

journal_title：Annals of laboratory medicine

authors： Shin BM,Lee EJ

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...

journal_title：Annals of laboratory medicine

authors： Ansari U,Behnes M,Hoffmann J,Natale M,Fastner C,El-Battrawy I,Rusnak J,Kim SH,Lang S,Hoffmann U,Bertsch T,Borggrefe M,Akin I

更新日期：2018-07-01 00:00:00