Development of an Automated Image Analyzer for Microvessel Density Measurement in Bone Marrow Biopsies.


:Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images of BM biopsies stained with anti-CD34 antibodies using two color models. MVD was calculated by merging images from the red and hue channels after eliminating non-microvessels. The analyzer results were compared with those obtained by two experienced hematopathologists in a blinded manner using the 84 BM samples of MM patients. Manual assessment of the MVD by two hematopathologists yielded mean±SD values of 19.4±11.8 and 20.0±11.8. The analyzer generated a mean±SD of 19.5±11.2. The intraclass correlation coefficient (ICC) and Bland-Altman plot of the MVD results demonstrated very good agreement between the automated image analyzer and both hematopathologists (ICC=0.893 [0.840-0.929] and ICC=0.906 [0.859-0.938]). This automated analyzer can provide time- and labor-saving benefits with more objective results in hematology laboratories.


Ann Lab Med


Chung Y,Shin S,Shim H,Sohn JY,Lee DE,Lee H,Eom HS,Kim KG,Kong SY




Has Abstract


2020-07-01 00:00:00














  • Plasma Levels and Diagnostic Utility of Macrophage Colony-Stimulating Factor, Matrix Metalloproteinase-9, and Tissue Inhibitor of Metalloproteinases-1 as New Biomarkers of Breast Cancer.

    abstract:BACKGROUND:Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and its specific tissue inhibitor - tissue inhibitor of metalloproteinases-1 (TIMP-1) may play an important role in the pathogenesis and spread of cancer. We investigated the plasma levels of M-CSF, MMP-9, and TIMP-1 in compari...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ławicki S,Głażewska EK,Sobolewska M,Będkowska GE,Szmitkowski M

    更新日期:2016-05-01 00:00:00

  • Performance of an Automated Fluorescence Antinuclear Antibody Image Analyzer.

    abstract:BACKGROUND:The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yoo IY,Oh JW,Cha HS,Koh EM,Kang ES

    更新日期:2017-05-01 00:00:00

  • Comparative Evaluation Between the RealStar Pneumocystis jirovecii PCR Kit and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR Kit for Detecting P. jirovecii in Non-HIV Immunocompromised Patients.

    abstract:BACKGROUND:Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (cari...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Huh HJ,Lim KR,Ki CS,Huh K,Shim HJ,Song DJ,Kim YJ,Chung DR,Lee NY

    更新日期:2019-03-01 00:00:00

  • Role of plasma exchange in ABO-incompatible kidney transplantation.

    abstract:BACKGROUND:In the past, ABO incompatibility was an absolute contraindication for solid organ transplantation. However, multiple recent trials have suggested strategies for overcoming the reactions between graft antigens and recipient antibodies that cause graft rejection. In this study, we determined the usefulness of ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yoo S,Lee EY,Huh KH,Kim MS,Kim YS,Kim HO

    更新日期:2012-07-01 00:00:00

  • Prediction of HLA-DQ in Deceased Donors and its Clinical Significance in Kidney Transplantation.

    abstract:Background:HLA-DQ typing in deceased donors is not mandatory in Korea. Therefore, when patients develop DQ antibodies after kidney transplantation (KT) from deceased donor, it is impossible to determine whether they are donor-specific antibodies (DSA). We developed DQ prediction programs for the HLA gene and evaluated ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Kim SK,Yang JJ,Hwang SH,Sung H,Shin S,Ko SY,Oh HB

    更新日期:2021-03-01 00:00:00

  • Budget Impact of the Accreditation Program for Clinical Laboratories on Colorectal Cancer Screening via Fecal Immunochemical Testing: Results from the National Cancer Screening Program in Korea.

    abstract:BACKGROUND:False-positive fecal immunochemical test (FIT) results in the National Cancer Screening Program (NCSP) for colorectal cancer may lead to unnecessary procedures, such as colonoscopies, increasing the medical costs. We estimated reductions in the cost of National Health Insurance according to the accreditation...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Jun JK,Sung NY,Song SH,Hong S,Jang MA,Song J,Kim JH,Min WK,Lee YK

    更新日期:2018-05-01 00:00:00

  • Interlaboratory Comparison of the Results of Lifecodes LSA Class I and Class II Single Antigen Kits for Human Leukocyte Antigen Antibody Detection.

    abstract:BACKGROUND:Although single antigen bead assays (SAB) are approved qualitative tests, the median fluorescence intensity (MFI) values obtained from SAB are frequently used in combination with quantitative significances for diagnostic purposes. To gauge the reproducibility of SAB results, we assessed the interlaboratory v...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Oh EJ,Park H,Park KU,Kang ES,Kim HS,Song EY

    更新日期:2015-05-01 00:00:00

  • Evaluation of the QuantaMatrix Multiplexed Assay Platform for Molecular Diagnosis of Multidrug- and Extensively Drug-Resistant Tuberculosis Using Clinical Strains Isolated in Myanmar.

    abstract:BACKGROUND:Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Chang Y,Kim S,Kim Y,Ei PW,Hwang D,Lee J,Chang CL,Lee H

    更新日期:2020-03-01 00:00:00

  • Stability of pH, Blood Gas Partial Pressure, Hemoglobin Oxygen Saturation Fraction, and Lactate Concentration.

    abstract:BACKGROUND:The storage temperature and time of blood gas samples collected in syringes constitute preanalytical variables that could affect blood gas or lactate concentration measurement results. We analyzed the effect of storage temperature and time delay on arterial or venous blood gas stability related to pH, partia...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Arbiol-Roca A,Imperiali CE,Dot-Bach D,Valero-Politi J,Dastis-Arias M

    更新日期:2020-11-01 00:00:00

  • Point of care D-dimer testing in the emergency department: a bioequivalence study.

    abstract:BACKGROUND:D-dimer is used widely as a diagnostic aid in low- and moderate-risk patients with suspected venous thromboembolism (VTE). While our laboratory utilizes VIDAS D-dimer analyzer (bioMérieux SA, France), our emergency department (ED) recently procured a D-dimer analyzer AQT90 FLEX (Radiometer Medical ApS, Denma...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Perveen S,Unwin D,Shetty AL

    更新日期:2013-01-01 00:00:00

  • The first Korean case of candidemia due to Candida dubliniensis.

    abstract::Candidemia due to uncommon Candida spp. appears to be increasing in incidence. C. dubliniensis has been increasingly recovered from individuals not infected with HIV. Identification of C. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to C. albicans. We report the first ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yu N,Kim HR,Lee MK

    更新日期:2012-05-01 00:00:00

  • Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.

    abstract:BACKGROUND:ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA). METHODS:A total of 530 fresh stool specimens were collec...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Shin BM,Lee EJ

    更新日期:2014-01-01 00:00:00

  • The impact of HLA and KIR ligand mismatching on unrelated allogeneic hematopoietic stem cell transplantation in Korean adult patients.

    abstract:BACKGROUND:The impact of HLA and KIR ligand mismatching on the outcome of hematopoietic stem cell transplantation (HSCT) remains unclear. Previous reports have identified considerable ethnic differences in the impact of HLA and KIR ligand mismatches, as well as KIR ligand status, on HSCT; however, to date, no data has ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Park H,Rho EY,In JW,Kim I,Yoon SS,Park S,Shin S,Park KU,Song EY

    更新日期:2015-01-01 00:00:00

  • Highly sensitive and novel point-of-care system, aQcare Chlamydia TRF kit for detecting Chlamydia trachomatis by using europium (Eu) (III) chelated nanoparticles.

    abstract:BACKGROUND:The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ham JY,Jung J,Hwang BG,Kim WJ,Kim YS,Kim EJ,Cho MY,Hwang MS,Won DI,Suh JS

    更新日期:2015-01-01 00:00:00

  • Morning Spot Urine Glucose-to-Creatinine Ratios Predict Overnight Urinary Glucose Excretion in Patients With Type 2 Diabetes.

    abstract:BACKGROUND:With the advent of sodium glucose co-transporter 2 inhibitors to control glucose and treat diabetes, laboratory data aided by either timed or spot glucose levels in the urine could be used as an alternative marker of drug response. The aim of this study was to assess the agreement between overnight urinary g...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Kim SR,Lee YH,Lee SG,Lee SH,Kang ES,Cha BS,Lee HC,Kim JH,Lee BW

    更新日期:2017-01-01 00:00:00

  • Biochemical and Genetic Analysis of Seven Korean Individuals With Suspected Metachromatic Leukodystrophy.

    abstract::Metachromatic leukodystrophy (MLD) is an autosomal recessive disease caused by a deficiency in arylsulfatase A (ARSA). However, decreased ARSA activity is also observed in pseudodeficiency (PD). To distinguish between MLD and PD, we performed gene mutation and sulfatide analyses by using dried blood spots (DBSs) from ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Han M,Jun SH,Lee YJ,Eun BL,Lee SJ,Seong MW,Park SS,Song SH,Park HD,Song J

    更新日期:2015-07-01 00:00:00

  • Prevalence of blaZ Gene and Performance of Phenotypic Tests to Detect Penicillinase in Staphylococcus aureus Isolates from Japan.

    abstract::Guidelines recommend that clinical laboratories perform phenotypic tests (nitrocefin-based test and penicillin 10-U [P10] or 1-U [P1] zone edge tests) to detect penicillinase in Staphylococcus aureus isolates. This study aimed to assess the prevalence of blaZ encoding penicillinase and perform various phenotypic tests...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Takayama Y,Tanaka T,Oikawa K,Fukano N,Goto M,Takahashi T

    更新日期:2018-03-01 00:00:00

  • Evaluation of PCR-reverse blot hybridization assay, REBA Sepsis-ID test, for simultaneous identification of bacterial pathogens and mecA and van genes from blood culture bottles.

    abstract:BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood cult...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Park SD,Lee G,Wang HY,Park M,Kim S,Kim H,Kim J,Kim YK,Kim HY,Lee H,Uh Y,Kim JB

    更新日期:2014-11-01 00:00:00

  • Application of the Whole Genome-Based Bacterial Identification System, TrueBac ID, Using Clinical Isolates That Were Not Identified With Three Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems.

    abstract:BACKGROUND:Next-generation sequencing is increasingly used for taxonomic identification of pathogenic bacterial isolates. We evaluated the performance of a newly introduced whole genome-based bacterial identification system, TrueBac ID (ChunLab Inc., Seoul, Korea), using clinical isolates that were not identified by th...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ha SM,Kim CK,Roh J,Byun JH,Yang SJ,Choi SB,Chun J,Yong D

    更新日期:2019-11-01 00:00:00

  • The Platelet-to-Lymphocyte Ratio as an Inflammatory Marker in Rheumatic Diseases.

    abstract::The platelet-to-lymphocyte ratio (PLR) has emerged as an informative marker revealing shifts in platelet and lymphocyte counts due to acute inflammatory and prothrombotic states. PLR has been extensively examined in neoplastic diseases accompanied by immune suppression and thrombosis, which can be predicted by combine...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章,评审


    authors: Gasparyan AY,Ayvazyan L,Mukanova U,Yessirkepov M,Kitas GD

    更新日期:2019-07-01 00:00:00

  • Delamanid, Bedaquiline, and Linezolid Minimum Inhibitory Concentration Distributions and Resistance-related Gene Mutations in Multidrug-resistant and Extensively Drug-resistant Tuberculosis in Korea.

    abstract:BACKGROUND:Delamanid, bedaquiline, and linezolid have recently been approved for the treatment of multidrug- and extensively drug-resistant (MDR and XDR, respectively) tuberculosis (TB). To use these drugs effectively, drug susceptibility tests, including rapid molecular techniques, are required for accurate diagnosis ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yang JS,Kim KJ,Choi H,Lee SH

    更新日期:2018-11-01 00:00:00

  • A case of IgM deficiency with B cell deficiency detected by ABO discrepancy in a patient with acute osteomyelitis.

    abstract::ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Jung CL,Cha MK,Jun BH,Hong KS

    更新日期:2013-05-01 00:00:00

  • Evaluation of Xpert Carba-R Assay v.2 to Detect Carbapenemase Genes in Two Hospitals in Korea.

    abstract:BACKGROUND:As the spread of carbapenemase-producing Enterobacteriaceae poses a critical threat to public health, rapid detection of carbapenemase genes is urgently required for prompt initiation of appropriate antimicrobial therapy and infection control. We evaluated the performance of Xpert Carba-R v.2 (Cepheid, USA) ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Byun JH,Kim YA,Kim M,Kim B,Choi JY,Park YS,Yong D

    更新日期:2020-05-01 00:00:00

  • Comparison of red blood cell hemolysis using plasma and serum separation tubes for outpatient specimens.

    abstract:BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST an...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ko DH,Won D,Jeong TD,Lee W,Chun S,Min WK

    更新日期:2015-03-01 00:00:00

  • Application of Multigene Panel Sequencing in Patients with Prolonged Rate-corrected QT Interval and No Pathogenic Variants Detected in KCNQ1, KCNH2, and SCN5A.

    abstract::Long QT syndrome (LQTS) is an inherited cardiac disease characterized by a prolonged heart rate-corrected QT (QTc) interval. We investigated the genetic causes in patients with prolonged QTc intervals who were negative for pathogenic variants in three major LQTS-related genes (KCNQ1, KCNH2, and SCN5A). Molecular genet...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Seo SH,Kim SY,Cho SI,Park H,Lee S,Choi JM,Kim MJ,Lee JS,Ahn KJ,Song MK,Bae EJ,Park SS,Seong MW

    更新日期:2018-01-01 00:00:00

  • Antibiotic resistance in Helicobacter pylori strains and its effect on H. pylori eradication rates in a single center in Korea.

    abstract:BACKGROUND:Clarithromycin, amoxicillin, metronidazole, tetracycline, and levofloxacin have been commonly used for the eradication of Helicobacter pylori. We compared the change in antibiotic resistance of H. pylori strains during two separate periods and investigated the effect of antibiotic resistance on H. pylori era...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: An B,Moon BS,Kim H,Lim HC,Lee YC,Lee G,Kim SH,Park M,Kim JB

    更新日期:2013-11-01 00:00:00

  • A Population-Based Genomic Study of Inherited Metabolic Diseases Detected Through Newborn Screening.

    abstract:BACKGROUND:A newborn screening (NBS) program has been utilized to detect asymptomatic newborns with inherited metabolic diseases (IMDs). There have been some bottlenecks such as false-positives and imprecision in the current NBS tests. To overcome these issues, we developed a multigene panel for IMD testing and investi...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Park KJ,Park S,Lee E,Park JH,Park JH,Park HD,Lee SY,Kim JW

    更新日期:2016-11-01 00:00:00

  • Effect of Irradiation on Microparticles in Red Blood Cell Concentrates.

    abstract::Changes in microparticles (MP) from red blood cell (RBC) concentrates in the context of irradiation have not been investigated. The aim of this study was to evaluate how irradiation affects the number of MPs within transfusion components. Twenty RBC concentrates, within 14 days after donation, were exposed to gamma ra...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Cho CH,Yun SG,Koh YE,Lim CS

    更新日期:2016-07-01 00:00:00

  • Establishing reference intervals for sex hormones on the analytical platforms Advia Centaur and Immulite 2000XP.

    abstract::Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 20...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Schüring AN,Kelsch R,Pierściński G,Nofer JR

    更新日期:2016-01-01 00:00:00

  • Platelet Function Analyzer-200 P2Y Results Are Predictive of the Risk of Major Adverse Cardiac Events in Korean Patients Receiving Clopidogrel Therapy Following Acute Coronary Syndrome.

    abstract:BACKGROUND:Clopidogrel is one of the most commonly used anti-platelet agents in cardiovascular diseases. We analyzed the relationship between the platelet function analyzer (PFA)-200 P2Y (INNOVANCE PFA-200 System, Siemens Healthcare, Germany) results and occurrence of major adverse cardiac events (MACEs) in Korean pati...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Lim HH,Li S,An GD,Woo KS,Kim KH,Kim JM,Kim MH,Han JY

    更新日期:2018-09-01 00:00:00