Abstract:
:Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images of BM biopsies stained with anti-CD34 antibodies using two color models. MVD was calculated by merging images from the red and hue channels after eliminating non-microvessels. The analyzer results were compared with those obtained by two experienced hematopathologists in a blinded manner using the 84 BM samples of MM patients. Manual assessment of the MVD by two hematopathologists yielded mean±SD values of 19.4±11.8 and 20.0±11.8. The analyzer generated a mean±SD of 19.5±11.2. The intraclass correlation coefficient (ICC) and Bland-Altman plot of the MVD results demonstrated very good agreement between the automated image analyzer and both hematopathologists (ICC=0.893 [0.840-0.929] and ICC=0.906 [0.859-0.938]). This automated analyzer can provide time- and labor-saving benefits with more objective results in hematology laboratories.
journal_name
Ann Lab Medjournal_title
Annals of laboratory medicineauthors
Chung Y,Shin S,Shim H,Sohn JY,Lee DE,Lee H,Eom HS,Kim KG,Kong SYdoi
10.3343/alm.2020.40.4.312subject
Has Abstractpub_date
2020-07-01 00:00:00pages
312-316issue
4eissn
2234-3806issn
2234-3814pii
40.312journal_volume
40pub_type
杂志文章abstract:BACKGROUND:Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and its specific tissue inhibitor - tissue inhibitor of metalloproteinases-1 (TIMP-1) may play an important role in the pathogenesis and spread of cancer. We investigated the plasma levels of M-CSF, MMP-9, and TIMP-1 in compari...
journal_title:Annals of laboratory medicine
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pub_type: 杂志文章
doi:10.3343/alm.2012.32.4.283
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journal_title:Annals of laboratory medicine
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journal_title:Annals of laboratory medicine
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journal_title:Annals of laboratory medicine
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doi:10.3343/alm.2020.40.2.142
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pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2012.32.3.225
更新日期:2012-05-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.1.15
更新日期:2014-01-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.1.111
更新日期:2015-01-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.1.50
更新日期:2015-01-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.1.9
更新日期:2017-01-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.4.458
更新日期:2015-07-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.2.155
更新日期:2018-03-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.6.446
更新日期:2014-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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更新日期:2016-01-01 00:00:00
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