Development of an Automated Image Analyzer for Microvessel Density Measurement in Bone Marrow Biopsies.

abstract：:The platelet-to-lymphocyte ratio (PLR) has emerged as an informative marker revealing shifts in platelet and lymphocyte counts due to acute inflammatory and prothrombotic states. PLR has been extensively examined in neoplastic diseases accompanied by immune suppression and thrombosis, which can be predicted by combine...

journal_title：Annals of laboratory medicine

authors： Gasparyan AY,Ayvazyan L,Mukanova U,Yessirkepov M,Kitas GD

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:We developed a single-color multitarget flow cytometry (SM-FC) assay, a single-tube assay with graded mean fluorescence intensities (MFIs). We evaluated the repeatability of SM-FC, and its correlation with multicolor flow cytometry (MFC), to assess its application as a routine FC assay. METHODS:We selected ...

journal_title：Annals of laboratory medicine

authors： Park J,Han K

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND:During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in Korea. We performed analytical and clinical validations of these kits. METH...

journal_title：Annals of laboratory medicine

authors： Kim MN,Ko YJ,Seong MW,Kim JS,Shin BM,Sung H

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Although Th2 immune activation is predominant in allergic diseases, neopterinlevels and indoleamine 2,3-dioxygenase (IDO)-1 activity (kynurenine:tryptophan ratio), which reflect Th1 immune activity, increase with interferon-gamma (IFN-γ) stimulation. We investigated neopterin, tryptophan, and kynurenine leve...

journal_title：Annals of laboratory medicine

authors： Ünüvar S,Erge D,Kılıçarslan B,Gözükara Bağ HG,Çatal F,Girgin G,Baydar T

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:The clindamycin-resistant erythromycin-susceptible (CRES) phenotype is rare in Streptococcus agalactiae (group B streptococci). We aimed to determine the molecular characteristics of CRES S. agalactiae using whole genome sequencing (WGS). METHODS:Sixty-six S. agalactiae isolates obtained from blood (N=26), ...

journal_title：Annals of laboratory medicine

authors： Takahashi T,Maeda T,Lee S,Lee DH,Kim S

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:This study was aimed to investigate the genetic diversity and antibiotic resistance profile of the nosocomial infection agent Acinetobacter baumannii from a medical intensive care unit (ICU) in a teaching hospital in Suzhou, China. METHODS:The genetic relationship among A. baumannii isolates in an ICU was i...

journal_title：Annals of laboratory medicine

authors： Zhong Q,Xu W,Wu Y,Xu H

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:D-dimer is used widely as a diagnostic aid in low- and moderate-risk patients with suspected venous thromboembolism (VTE). While our laboratory utilizes VIDAS D-dimer analyzer (bioMérieux SA, France), our emergency department (ED) recently procured a D-dimer analyzer AQT90 FLEX (Radiometer Medical ApS, Denma...

journal_title：Annals of laboratory medicine

authors： Perveen S,Unwin D,Shetty AL

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:Many laboratories use 4 delta check methods: delta difference, delta percent change, rate difference, and rate percent change. However, guidelines regarding decision criteria for selecting delta check methods have not yet been provided. We present new decision criteria for selecting delta check methods for e...

journal_title：Annals of laboratory medicine

authors： Park SH,Kim SY,Lee W,Chun S,Min WK

更新日期：2012-09-01 00:00:00

abstract：:Metachromatic leukodystrophy (MLD) is an autosomal recessive disease caused by a deficiency in arylsulfatase A (ARSA). However, decreased ARSA activity is also observed in pseudodeficiency (PD). To distinguish between MLD and PD, we performed gene mutation and sulfatide analyses by using dried blood spots (DBSs) from ...

journal_title：Annals of laboratory medicine

authors： Han M,Jun SH,Lee YJ,Eun BL,Lee SJ,Seong MW,Park SS,Song SH,Park HD,Song J

更新日期：2015-07-01 00:00:00

abstract：BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood cult...

journal_title：Annals of laboratory medicine

authors： Park SD,Lee G,Wang HY,Park M,Kim S,Kim H,Kim J,Kim YK,Kim HY,Lee H,Uh Y,Kim JB

更新日期：2014-11-01 00:00:00

abstract：:We compared the activities of tedizolid to those of linezolid and other commonly used antimicrobial agents against gram-positive cocci recovered from patients with skin and skin structure infections (SSSIs) and hospital-acquired pneumonia (HAP) in Korean hospitals. Gram-positive isolates were collected from 356 patien...

journal_title：Annals of laboratory medicine

authors： Lee Y,Hong SK,Choi S,Im W,Yong D,Lee K

更新日期：2015-09-01 00:00:00

abstract：:Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 20...

journal_title：Annals of laboratory medicine

authors： Schüring AN,Kelsch R,Pierściński G,Nofer JR

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND:Next-generation sequencing is increasingly used for taxonomic identification of pathogenic bacterial isolates. We evaluated the performance of a newly introduced whole genome-based bacterial identification system, TrueBac ID (ChunLab Inc., Seoul, Korea), using clinical isolates that were not identified by th...

journal_title：Annals of laboratory medicine

authors： Ha SM,Kim CK,Roh J,Byun JH,Yang SJ,Choi SB,Chun J,Yong D

更新日期：2019-11-01 00:00:00

abstract：BACKGROUND:We reviewed patients with multiple myeloma (MM) in order to assess the incidence of genetic abnormalities and their associations with clinical parameters, risk groups, and prognosis. METHODS:A total of 130 patients with MM were enrolled. The incidences of genetic abnormalities were determined in all patient...

journal_title：Annals of laboratory medicine

authors： Jekarl DW,Min CK,Kwon A,Kim H,Chae H,Kim M,Lim J,Kim Y,Han K

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND:Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequ...

journal_title：Annals of laboratory medicine

authors： Jeong JH,Lee HT,Seo JY,Seo YH,Kim KH,Kim MJ,Lee JH,Park J,Hong JS,Park PW,Ahn JY

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:The storage temperature and time of blood gas samples collected in syringes constitute preanalytical variables that could affect blood gas or lactate concentration measurement results. We analyzed the effect of storage temperature and time delay on arterial or venous blood gas stability related to pH, partia...

journal_title：Annals of laboratory medicine

authors： Arbiol-Roca A,Imperiali CE,Dot-Bach D,Valero-Politi J,Dastis-Arias M

更新日期：2020-11-01 00:00:00

abstract：BACKGROUND:Neutrophil gelatinase-associated lipocalin (NGAL) is a useful biomarker for acute kidney injury (AKI) prediction. However, studies on whether using both plasma NGAL (PNGAL) and urine NGAL (UNGAL) can improve AKI prediction are limited. We investigated the best approach to predict AKI in high-risk patients wh...

journal_title：Annals of laboratory medicine

authors： Yi A,Lee CH,Yun YM,Kim H,Moon HW,Hur M

更新日期：2021-01-01 00:00:00

abstract：BACKGROUND:The Automated Fluorescent Immunoassay System (AFIAS) rotavirus assay (Boditech Med Inc., Chuncheon, Korea) is a new rapid antigen test for rotavirus detection. We evaluated the performance of this assay for detecting rotaviruses and their specific genotypes in clinical stool samples. METHODS:AFIAS rotavirus...

journal_title：Annals of laboratory medicine

authors： Kim JS,Lee SK,Ko DH,Hyun J,Kim HS

更新日期：2019-01-01 00:00:00

abstract：:ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...

journal_title：Annals of laboratory medicine

authors： Jung CL,Cha MK,Jun BH,Hong KS

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND:The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flex...

journal_title：Annals of laboratory medicine

authors： Liu Y,Cheng Y,Yang H,Hu L,Cheng J,Ye Y,Li J

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...

journal_title：Annals of laboratory medicine

authors： Ansari U,Behnes M,Hoffmann J,Natale M,Fastner C,El-Battrawy I,Rusnak J,Kim SH,Lang S,Hoffmann U,Bertsch T,Borggrefe M,Akin I

更新日期：2018-07-01 00:00:00

abstract：:Procalcitonin (PCT) is used as a biomarker for the diagnosis of sepsis, severe sepsis and septic shock. At the same time, PCT has also been used to guide antibiotic therapy. This review outlines the main indications for PCT measurement and points out possible pitfalls. The classic indications for PCT measurement are: ...

journal_title：Annals of laboratory medicine

authors： Meisner M

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:Clopidogrel is one of the most commonly used anti-platelet agents in cardiovascular diseases. We analyzed the relationship between the platelet function analyzer (PFA)-200 P2Y (INNOVANCE PFA-200 System, Siemens Healthcare, Germany) results and occurrence of major adverse cardiac events (MACEs) in Korean pati...

journal_title：Annals of laboratory medicine

authors： Lim HH,Li S,An GD,Woo KS,Kim KH,Kim JM,Kim MH,Han JY

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:As the spread of carbapenemase-producing Enterobacteriaceae poses a critical threat to public health, rapid detection of carbapenemase genes is urgently required for prompt initiation of appropriate antimicrobial therapy and infection control. We evaluated the performance of Xpert Carba-R v.2 (Cepheid, USA) ...

journal_title：Annals of laboratory medicine

authors： Byun JH,Kim YA,Kim M,Kim B,Choi JY,Park YS,Yong D

更新日期：2020-05-01 00:00:00

abstract：BACKGROUND:Although single antigen bead assays (SAB) are approved qualitative tests, the median fluorescence intensity (MFI) values obtained from SAB are frequently used in combination with quantitative significances for diagnostic purposes. To gauge the reproducibility of SAB results, we assessed the interlaboratory v...

journal_title：Annals of laboratory medicine

authors： Oh EJ,Park H,Park KU,Kang ES,Kim HS,Song EY

更新日期：2015-05-01 00:00:00

abstract：BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST an...

journal_title：Annals of laboratory medicine

authors： Ko DH,Won D,Jeong TD,Lee W,Chun S,Min WK

更新日期：2015-03-01 00:00:00

abstract：:Candidemia due to uncommon Candida spp. appears to be increasing in incidence. C. dubliniensis has been increasingly recovered from individuals not infected with HIV. Identification of C. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to C. albicans. We report the first ...

journal_title：Annals of laboratory medicine

authors： Yu N,Kim HR,Lee MK

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND:Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread...

journal_title：Annals of laboratory medicine

authors： Mazalovska M,Varadinov N,Koynarski T,Minkov I,Teoharov P,Lomonossoff GP,Zahmanova G

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:The impact of HLA and KIR ligand mismatching on the outcome of hematopoietic stem cell transplantation (HSCT) remains unclear. Previous reports have identified considerable ethnic differences in the impact of HLA and KIR ligand mismatches, as well as KIR ligand status, on HSCT; however, to date, no data has ...

journal_title：Annals of laboratory medicine

authors： Park H,Rho EY,In JW,Kim I,Yoon SS,Park S,Shin S,Park KU,Song EY

更新日期：2015-01-01 00:00:00

abstract：:There have been a number of studies about correlations between HLA genotypes in various ethnic groups and occurrence of various cutaneous adverse drug reactions, ranging in intensity from mild to severe, caused by antiepileptic drugs (AEDs). This is the first report analyzing the HLA genotypes of 9 Korean patients wit...

journal_title：Annals of laboratory medicine

authors： Song JS,Kang ES,Joo EY,Hong SB,Seo DW,Lee SY

更新日期：2014-09-01 00:00:00