Development of an Automated Image Analyzer for Microvessel Density Measurement in Bone Marrow Biopsies.

Abstract:

:Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images of BM biopsies stained with anti-CD34 antibodies using two color models. MVD was calculated by merging images from the red and hue channels after eliminating non-microvessels. The analyzer results were compared with those obtained by two experienced hematopathologists in a blinded manner using the 84 BM samples of MM patients. Manual assessment of the MVD by two hematopathologists yielded mean±SD values of 19.4±11.8 and 20.0±11.8. The analyzer generated a mean±SD of 19.5±11.2. The intraclass correlation coefficient (ICC) and Bland-Altman plot of the MVD results demonstrated very good agreement between the automated image analyzer and both hematopathologists (ICC=0.893 [0.840-0.929] and ICC=0.906 [0.859-0.938]). This automated analyzer can provide time- and labor-saving benefits with more objective results in hematology laboratories.

journal_name

Ann Lab Med

authors

Chung Y,Shin S,Shim H,Sohn JY,Lee DE,Lee H,Eom HS,Kim KG,Kong SY

doi

10.3343/alm.2020.40.4.312

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

312-316

issue

4

eissn

2234-3806

issn

2234-3814

pii

40.312

journal_volume

40

pub_type

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