Development of an Automated Image Analyzer for Microvessel Density Measurement in Bone Marrow Biopsies.


:Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images of BM biopsies stained with anti-CD34 antibodies using two color models. MVD was calculated by merging images from the red and hue channels after eliminating non-microvessels. The analyzer results were compared with those obtained by two experienced hematopathologists in a blinded manner using the 84 BM samples of MM patients. Manual assessment of the MVD by two hematopathologists yielded mean±SD values of 19.4±11.8 and 20.0±11.8. The analyzer generated a mean±SD of 19.5±11.2. The intraclass correlation coefficient (ICC) and Bland-Altman plot of the MVD results demonstrated very good agreement between the automated image analyzer and both hematopathologists (ICC=0.893 [0.840-0.929] and ICC=0.906 [0.859-0.938]). This automated analyzer can provide time- and labor-saving benefits with more objective results in hematology laboratories.


Ann Lab Med


Chung Y,Shin S,Shim H,Sohn JY,Lee DE,Lee H,Eom HS,Kim KG,Kong SY




Has Abstract


2020-07-01 00:00:00














  • Comparative Evaluation Between the RealStar Pneumocystis jirovecii PCR Kit and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR Kit for Detecting P. jirovecii in Non-HIV Immunocompromised Patients.

    abstract:BACKGROUND:Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (cari...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Huh HJ,Lim KR,Ki CS,Huh K,Shim HJ,Song DJ,Kim YJ,Chung DR,Lee NY

    更新日期:2019-03-01 00:00:00

  • Stability of pH, Blood Gas Partial Pressure, Hemoglobin Oxygen Saturation Fraction, and Lactate Concentration.

    abstract:BACKGROUND:The storage temperature and time of blood gas samples collected in syringes constitute preanalytical variables that could affect blood gas or lactate concentration measurement results. We analyzed the effect of storage temperature and time delay on arterial or venous blood gas stability related to pH, partia...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Arbiol-Roca A,Imperiali CE,Dot-Bach D,Valero-Politi J,Dastis-Arias M

    更新日期:2020-11-01 00:00:00

  • Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR.

    abstract:BACKGROUND:During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in Korea. We performed analytical and clinical validations of these kits. METH...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Kim MN,Ko YJ,Seong MW,Kim JS,Shin BM,Sung H

    更新日期:2016-09-01 00:00:00

  • Impact of genetic abnormalities on the prognoses and clinical parameters of patients with multiple myeloma.

    abstract:BACKGROUND:We reviewed patients with multiple myeloma (MM) in order to assess the incidence of genetic abnormalities and their associations with clinical parameters, risk groups, and prognosis. METHODS:A total of 130 patients with MM were enrolled. The incidences of genetic abnormalities were determined in all patient...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Jekarl DW,Min CK,Kwon A,Kim H,Chae H,Kim M,Lim J,Kim Y,Han K

    更新日期:2013-07-01 00:00:00

  • Antibiotic resistance in Helicobacter pylori strains and its effect on H. pylori eradication rates in a single center in Korea.

    abstract:BACKGROUND:Clarithromycin, amoxicillin, metronidazole, tetracycline, and levofloxacin have been commonly used for the eradication of Helicobacter pylori. We compared the change in antibiotic resistance of H. pylori strains during two separate periods and investigated the effect of antibiotic resistance on H. pylori era...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: An B,Moon BS,Kim H,Lim HC,Lee YC,Lee G,Kim SH,Park M,Kim JB

    更新日期:2013-11-01 00:00:00

  • Delamanid, Bedaquiline, and Linezolid Minimum Inhibitory Concentration Distributions and Resistance-related Gene Mutations in Multidrug-resistant and Extensively Drug-resistant Tuberculosis in Korea.

    abstract:BACKGROUND:Delamanid, bedaquiline, and linezolid have recently been approved for the treatment of multidrug- and extensively drug-resistant (MDR and XDR, respectively) tuberculosis (TB). To use these drugs effectively, drug susceptibility tests, including rapid molecular techniques, are required for accurate diagnosis ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yang JS,Kim KJ,Choi H,Lee SH

    更新日期:2018-11-01 00:00:00

  • Evaluation of the QuantaMatrix Multiplexed Assay Platform for Molecular Diagnosis of Multidrug- and Extensively Drug-Resistant Tuberculosis Using Clinical Strains Isolated in Myanmar.

    abstract:BACKGROUND:Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Chang Y,Kim S,Kim Y,Ei PW,Hwang D,Lee J,Chang CL,Lee H

    更新日期:2020-03-01 00:00:00

  • Budget Impact of the Accreditation Program for Clinical Laboratories on Colorectal Cancer Screening via Fecal Immunochemical Testing: Results from the National Cancer Screening Program in Korea.

    abstract:BACKGROUND:False-positive fecal immunochemical test (FIT) results in the National Cancer Screening Program (NCSP) for colorectal cancer may lead to unnecessary procedures, such as colonoscopies, increasing the medical costs. We estimated reductions in the cost of National Health Insurance according to the accreditation...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Jun JK,Sung NY,Song SH,Hong S,Jang MA,Song J,Kim JH,Min WK,Lee YK

    更新日期:2018-05-01 00:00:00

  • Evaluation of Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile Assays for Direct Detection of Toxigenic Clostridium difficile in Stool Specimens.

    abstract:BACKGROUND:We evaluated the performance of four commercial nucleic acid amplification tests (NAATs: Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile) for direct and rapid detection of Clostridium difficile toxin genes. METHODS:We compared four NAATs on the same set of 3...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Shin BM,Yoo SM,Shin WC

    更新日期:2016-03-01 00:00:00

  • Plasma Levels and Diagnostic Utility of Macrophage Colony-Stimulating Factor, Matrix Metalloproteinase-9, and Tissue Inhibitor of Metalloproteinases-1 as New Biomarkers of Breast Cancer.

    abstract:BACKGROUND:Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and its specific tissue inhibitor - tissue inhibitor of metalloproteinases-1 (TIMP-1) may play an important role in the pathogenesis and spread of cancer. We investigated the plasma levels of M-CSF, MMP-9, and TIMP-1 in compari...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ławicki S,Głażewska EK,Sobolewska M,Będkowska GE,Szmitkowski M

    更新日期:2016-05-01 00:00:00

  • Neopterin Levels and Indoleamine 2,3-Dioxygenase Activity as Biomarkers of Immune System Activation and Childhood Allergic Diseases.

    abstract:BACKGROUND:Although Th2 immune activation is predominant in allergic diseases, neopterinlevels and indoleamine 2,3-dioxygenase (IDO)-1 activity (kynurenine:tryptophan ratio), which reflect Th1 immune activity, increase with interferon-gamma (IFN-γ) stimulation. We investigated neopterin, tryptophan, and kynurenine leve...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ünüvar S,Erge D,Kılıçarslan B,Gözükara Bağ HG,Çatal F,Girgin G,Baydar T

    更新日期:2019-05-01 00:00:00

  • Accessory Gene Regulator Polymorphism and Vancomycin Minimum Inhibitory Concentration in Methicillin-Resistant Staphylococcus aureus.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia with a vancomycin minimum inhibitory concentration (MIC) of 2 µg/mL presents a high rate of therapeutic failure in response to vancomycin. In addition, polymorphism in accessory gene regulator (agr) is associated with vancomycin therapeutic effect...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Park MJ,Kim HS,Kim HS,Kim JS,Song W,Kim MY,Lee YK,Kang HJ

    更新日期:2015-07-01 00:00:00

  • Erratum: COVID-19 Molecular Testing in Korea: Practical Essentials and Answers From Experts Based on Experiences of Emergency Use Authorization Assays.

    abstract::[This corrects the article on p. 439 in vol. 40.]. ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章,已发布勘误


    authors: Sung H,Roh KH,Hong KH,Seong MW,Ryoo N,Kim HS,Lee J,Kim SY,Ryu SW,Kim MN,Han MG,Lee SW,Lee H,Yoo CK

    更新日期:2021-01-01 00:00:00

  • Clinical and microbiological characteristics of six Staphylococcus pettenkoferi isolates from blood samples.

    abstract::Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood speci...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Park S,Chung HS,Lee M

    更新日期:2015-03-01 00:00:00

  • Linezolid Resistance in Methicillin-Resistant Staphylococcus aureus in Korea: High Rate of False Resistance to Linezolid by the VITEK 2 System.

    abstract::As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven MRSA...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yoo IY,Kang OK,Shim HJ,Huh HJ,Lee NY

    更新日期:2020-01-01 00:00:00

  • A case of IgM deficiency with B cell deficiency detected by ABO discrepancy in a patient with acute osteomyelitis.

    abstract::ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Jung CL,Cha MK,Jun BH,Hong KS

    更新日期:2013-05-01 00:00:00

  • The 2016 WHO versus 2008 WHO Criteria for the Diagnosis of Chronic Myelomonocytic Leukemia.

    abstract::The 2016 WHO diagnostic criteria for chronic myelomonocytic leukemia (CMML) require both absolute and relative monocytosis (≥1×10⁹/L and ≥10% of white blood cell counts) in peripheral blood. Moreover, myeloproliferative neoplasm (MPN) features in bone marrow and/or MPN-associated mutations tend to support MPN with mon...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Moon Y,Kim MH,Kim HR,Ahn JY,Huh J,Huh JY,Han JH,Park JS,Cho SR

    更新日期:2018-09-01 00:00:00

  • Characterization of Extended-Spectrum β-Lactamase Genes of Shigella flexneri Isolates With Fosfomycin Resistance From Patients in China.

    abstract:BACKGROUND:The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flex...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Liu Y,Cheng Y,Yang H,Hu L,Cheng J,Ye Y,Li J

    更新日期:2017-09-01 00:00:00

  • Screening PCR Versus Sanger Sequencing: Detection of CALR Mutations in Patients With Thrombocytosis.

    abstract:BACKGROUND:Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Jeong JH,Lee HT,Seo JY,Seo YH,Kim KH,Kim MJ,Lee JH,Park J,Hong JS,Park PW,Ahn JY

    更新日期:2016-07-01 00:00:00

  • Performance of an Automated Fluorescence Antinuclear Antibody Image Analyzer.

    abstract:BACKGROUND:The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yoo IY,Oh JW,Cha HS,Koh EM,Kang ES

    更新日期:2017-05-01 00:00:00

  • Evaluation of PCR-reverse blot hybridization assay, REBA Sepsis-ID test, for simultaneous identification of bacterial pathogens and mecA and van genes from blood culture bottles.

    abstract:BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood cult...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Park SD,Lee G,Wang HY,Park M,Kim S,Kim H,Kim J,Kim YK,Kim HY,Lee H,Uh Y,Kim JB

    更新日期:2014-11-01 00:00:00

  • A Population-Based Genomic Study of Inherited Metabolic Diseases Detected Through Newborn Screening.

    abstract:BACKGROUND:A newborn screening (NBS) program has been utilized to detect asymptomatic newborns with inherited metabolic diseases (IMDs). There have been some bottlenecks such as false-positives and imprecision in the current NBS tests. To overcome these issues, we developed a multigene panel for IMD testing and investi...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Park KJ,Park S,Lee E,Park JH,Park JH,Park HD,Lee SY,Kim JW

    更新日期:2016-11-01 00:00:00

  • Role of plasma exchange in ABO-incompatible kidney transplantation.

    abstract:BACKGROUND:In the past, ABO incompatibility was an absolute contraindication for solid organ transplantation. However, multiple recent trials have suggested strategies for overcoming the reactions between graft antigens and recipient antibodies that cause graft rejection. In this study, we determined the usefulness of ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Yoo S,Lee EY,Huh KH,Kim MS,Kim YS,Kim HO

    更新日期:2012-07-01 00:00:00

  • Point of care D-dimer testing in the emergency department: a bioequivalence study.

    abstract:BACKGROUND:D-dimer is used widely as a diagnostic aid in low- and moderate-risk patients with suspected venous thromboembolism (VTE). While our laboratory utilizes VIDAS D-dimer analyzer (bioMérieux SA, France), our emergency department (ED) recently procured a D-dimer analyzer AQT90 FLEX (Radiometer Medical ApS, Denma...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Perveen S,Unwin D,Shetty AL

    更新日期:2013-01-01 00:00:00

  • Morning Spot Urine Glucose-to-Creatinine Ratios Predict Overnight Urinary Glucose Excretion in Patients With Type 2 Diabetes.

    abstract:BACKGROUND:With the advent of sodium glucose co-transporter 2 inhibitors to control glucose and treat diabetes, laboratory data aided by either timed or spot glucose levels in the urine could be used as an alternative marker of drug response. The aim of this study was to assess the agreement between overnight urinary g...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Kim SR,Lee YH,Lee SG,Lee SH,Kang ES,Cha BS,Lee HC,Kim JH,Lee BW

    更新日期:2017-01-01 00:00:00

  • Prevalence of blaZ Gene and Performance of Phenotypic Tests to Detect Penicillinase in Staphylococcus aureus Isolates from Japan.

    abstract::Guidelines recommend that clinical laboratories perform phenotypic tests (nitrocefin-based test and penicillin 10-U [P10] or 1-U [P1] zone edge tests) to detect penicillinase in Staphylococcus aureus isolates. This study aimed to assess the prevalence of blaZ encoding penicillinase and perform various phenotypic tests...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Takayama Y,Tanaka T,Oikawa K,Fukano N,Goto M,Takahashi T

    更新日期:2018-03-01 00:00:00

  • Use of PCR with sequence-specific primers for high-resolution human leukocyte antigen typing of patients with narcolepsy.

    abstract:BACKGROUND:Narcolepsy is a neurologic disorder characterized by excessive daytime sleepiness, symptoms of abnormal rapid eye movement (REM) sleep, and a strong association with HLA-DRB1*1501, -DQA1*0102, and -DQB1*0602. Here, we investigated the clinico-physical characteristics of Korean patients with narcolepsy, their...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Woo HI,Joo EY,Hong SB,Lee KW,Kang ES

    更新日期:2012-01-01 00:00:00

  • Comparison of red blood cell hemolysis using plasma and serum separation tubes for outpatient specimens.

    abstract:BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST an...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ko DH,Won D,Jeong TD,Lee W,Chun S,Min WK

    更新日期:2015-03-01 00:00:00

  • Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.

    abstract:BACKGROUND:ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA). METHODS:A total of 530 fresh stool specimens were collec...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Shin BM,Lee EJ

    更新日期:2014-01-01 00:00:00

  • Galectin-3 Reflects the Echocardiographic Grades of Left Ventricular Diastolic Dysfunction.

    abstract:BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章


    authors: Ansari U,Behnes M,Hoffmann J,Natale M,Fastner C,El-Battrawy I,Rusnak J,Kim SH,Lang S,Hoffmann U,Bertsch T,Borggrefe M,Akin I

    更新日期:2018-07-01 00:00:00