Abstract:
BACKGROUND:ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA). METHODS:A total of 530 fresh stool specimens were collected from patients with clinical signs compatible with C. difficile infection, and cultures for C. difficile were performed on IDCd and CDSA. C. difficile colonies were identified by spore staining, odor, use of an ANI identification test kit (bioMérieux SA), and multiplex PCR for tcdA, tcdB, and tpi. RESULTS:The concordance rate between IDCd and CDSA was 90.6% (480/530). The positivity rates on IDCd on days 1 and 2 (55.6% and 85.0%, respectively) were significantly higher than those on CDSA (19.4% and 75.6%, respectively) (P<0.001 for day 1 and P=0.02 for day 2), but the detection rates on IDCd and CDSA on day 3 were not different (89.4% vs. 82.8%, P=0.0914). On day 3, the recovery rates for non-C. difficile isolates on IDCd and CDSA were 30.2% (160/530) and 22.1% (117/530), respectively (P=0.0075). Clostridium spp. other than C. difficile were the most prevalent non-C. difficile isolates on both media. CONCLUSIONS:The culture positivity rates on IDCd and CDSA were not different on day 3 but IDCd may allow for rapid and sensitive detection of C. difficile within 2 days of cultivation.
journal_name
Ann Lab Medjournal_title
Annals of laboratory medicineauthors
Shin BM,Lee EJdoi
10.3343/alm.2014.34.1.15subject
Has Abstractpub_date
2014-01-01 00:00:00pages
15-9issue
1eissn
2234-3806issn
2234-3814journal_volume
34pub_type
杂志文章abstract:BACKGROUND:The Automated Fluorescent Immunoassay System (AFIAS) rotavirus assay (Boditech Med Inc., Chuncheon, Korea) is a new rapid antigen test for rotavirus detection. We evaluated the performance of this assay for detecting rotaviruses and their specific genotypes in clinical stool samples. METHODS:AFIAS rotavirus...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2019.39.1.50
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:The clindamycin-resistant erythromycin-susceptible (CRES) phenotype is rare in Streptococcus agalactiae (group B streptococci). We aimed to determine the molecular characteristics of CRES S. agalactiae using whole genome sequencing (WGS). METHODS:Sixty-six S. agalactiae isolates obtained from blood (N=26), ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.5.370
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a rare inherited disorder characterized by infantile-onset macrocephaly, slow neurologic deterioration, and seizures. Mutations in the causative gene, MLC1, are found in approximately 75% of patients and are inherited in an autosomal recessi...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.6.516
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:We reviewed patients with multiple myeloma (MM) in order to assess the incidence of genetic abnormalities and their associations with clinical parameters, risk groups, and prognosis. METHODS:A total of 130 patients with MM were enrolled. The incidences of genetic abnormalities were determined in all patient...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2013.33.4.248
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.4.313
更新日期:2017-07-01 00:00:00
abstract::Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.4.312
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:D-dimer is used widely as a diagnostic aid in low- and moderate-risk patients with suspected venous thromboembolism (VTE). While our laboratory utilizes VIDAS D-dimer analyzer (bioMérieux SA, France), our emergency department (ED) recently procured a D-dimer analyzer AQT90 FLEX (Radiometer Medical ApS, Denma...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2013.33.1.34
更新日期:2013-01-01 00:00:00
abstract::As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven MRSA...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.1.57
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Although single antigen bead assays (SAB) are approved qualitative tests, the median fluorescence intensity (MFI) values obtained from SAB are frequently used in combination with quantitative significances for diagnostic purposes. To gauge the reproducibility of SAB results, we assessed the interlaboratory v...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.3.321
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in Korea. We performed analytical and clinical validations of these kits. METH...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.5.450
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:As the spread of carbapenemase-producing Enterobacteriaceae poses a critical threat to public health, rapid detection of carbapenemase genes is urgently required for prompt initiation of appropriate antimicrobial therapy and infection control. We evaluated the performance of Xpert Carba-R v.2 (Cepheid, USA) ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2020.40.3.209
更新日期:2020-05-01 00:00:00
abstract:Background:Plasma cell myeloma (PCM) is caused by immune dysregulation. We evaluated the expression of immune checkpoint programmed cell death protein-1 (PD-1) on T cell subsets in PCM patients according to disease course and cytogenetic abnormalities. This study aimed to find a target group suitable for therapeutic us...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2021.41.3.259
更新日期:2021-05-01 00:00:00
abstract::We compared the activities of tedizolid to those of linezolid and other commonly used antimicrobial agents against gram-positive cocci recovered from patients with skin and skin structure infections (SSSIs) and hospital-acquired pneumonia (HAP) in Korean hospitals. Gram-positive isolates were collected from 356 patien...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.5.523
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.4.306
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:We evaluated the performance of four commercial nucleic acid amplification tests (NAATs: Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile) for direct and rapid detection of Clostridium difficile toxin genes. METHODS:We compared four NAATs on the same set of 3...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.2.131
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.3.240
更新日期:2017-05-01 00:00:00
abstract::Candidemia due to uncommon Candida spp. appears to be increasing in incidence. C. dubliniensis has been increasingly recovered from individuals not infected with HIV. Identification of C. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to C. albicans. We report the first ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2012.32.3.225
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia with a vancomycin minimum inhibitory concentration (MIC) of 2 µg/mL presents a high rate of therapeutic failure in response to vancomycin. In addition, polymorphism in accessory gene regulator (agr) is associated with vancomycin therapeutic effect...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.4.399
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Many laboratories use 4 delta check methods: delta difference, delta percent change, rate difference, and rate percent change. However, guidelines regarding decision criteria for selecting delta check methods have not yet been provided. We present new decision criteria for selecting delta check methods for e...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2012.32.5.345
更新日期:2012-09-01 00:00:00
abstract:Background:HLA-DQ typing in deceased donors is not mandatory in Korea. Therefore, when patients develop DQ antibodies after kidney transplantation (KT) from deceased donor, it is impossible to determine whether they are donor-specific antibodies (DSA). We developed DQ prediction programs for the HLA gene and evaluated ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2021.41.2.190
更新日期:2021-03-01 00:00:00
abstract:BACKGROUND:High-sensitivity cardiac troponin I (hs-cTnI) and the soluble isoform of suppression of tumorigenicity 2 (sST2) are useful prognostic biomarkers in acute coronary syndrome (ACS). The aim of this study was to test the short term prognostic value of sST2 compared with hs-cTnI in patients with chest pain. METH...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.2.137
更新日期:2017-03-01 00:00:00
abstract::Procalcitonin (PCT) is used as a biomarker for the diagnosis of sepsis, severe sepsis and septic shock. At the same time, PCT has also been used to guide antibiotic therapy. This review outlines the main indications for PCT measurement and points out possible pitfalls. The classic indications for PCT measurement are: ...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
doi:10.3343/alm.2014.34.4.263
更新日期:2014-07-01 00:00:00
abstract::Myocardial infarction (MI) is the leading cause of death in the developed world. Biomarkers have an essential role in diagnosis, risk stratification, guiding management and clinical decision making in the setting of patients presenting with signs and symptoms of MI. Cardiac troponin (cTn) rose to prominence during the...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
doi:10.3343/alm.2013.33.5.309
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND:Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (cari...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2019.39.2.176
更新日期:2019-03-01 00:00:00
abstract::Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 20...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.1.55
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.1.50
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Clopidogrel is one of the most commonly used anti-platelet agents in cardiovascular diseases. We analyzed the relationship between the platelet function analyzer (PFA)-200 P2Y (INNOVANCE PFA-200 System, Siemens Healthcare, Germany) results and occurrence of major adverse cardiac events (MACEs) in Korean pati...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2018.38.5.413
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:With the advent of sodium glucose co-transporter 2 inhibitors to control glucose and treat diabetes, laboratory data aided by either timed or spot glucose levels in the urine could be used as an alternative marker of drug response. The aim of this study was to assess the agreement between overnight urinary g...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.1.9
更新日期:2017-01-01 00:00:00
abstract::The platelet-to-lymphocyte ratio (PLR) has emerged as an informative marker revealing shifts in platelet and lymphocyte counts due to acute inflammatory and prothrombotic states. PLR has been extensively examined in neoplastic diseases accompanied by immune suppression and thrombosis, which can be predicted by combine...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
doi:10.3343/alm.2019.39.4.345
更新日期:2019-07-01 00:00:00
abstract::Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood speci...
journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.2.250
更新日期:2015-03-01 00:00:00