Abstract:
:As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven MRSA isolates from 14 patients exhibiting linezolid MICs ≥8 μg/mL were examined by broth microdilution (BMD) test as well as by sequencing for mutations in the 23S rRNA gene or ribosomal proteins (L3, L4, and L22) and the presence of the optrA, cfr, and cfr(B) genes. Of the 27 isolates, four (14.8%) from one patient were confirmed as linezolid resistant by BMD and harbored a 23S rRNA T2500A mutation. The remaining 23 were confirmed as linezolid susceptible, indicating that the linezolid-resistant results were major errors generated by VITEK 2. The most commonly detected mutation (19/27, 70.4%), L3 Gly152Asp, was detected in only linezolid-susceptible isolates. No isolates contained optrA, cfr, or cfr(B) or any L4 or L22 protein alterations. Our results show that the 23S rRNA T2500A mutation was mainly associated with linezolid resistance, while the L3 Gly152Asp mutation was not related to linezolid resistance. A confirmatory test is recommended for VITEK 2 linezolid-resistant results owing to the high probability of false resistant results.
journal_name
Ann Lab Medjournal_title
Annals of laboratory medicineauthors
Yoo IY,Kang OK,Shim HJ,Huh HJ,Lee NYdoi
10.3343/alm.2020.40.1.57subject
Has Abstractpub_date
2020-01-01 00:00:00pages
57-62issue
1eissn
2234-3806issn
2234-3814pii
40.57journal_volume
40pub_type
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pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
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journal_title:Annals of laboratory medicine
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2015.35.2.250
更新日期:2015-03-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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更新日期:2015-07-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2012.32.1.57
更新日期:2012-01-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2016.36.3.223
更新日期:2016-05-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2012.32.6.413
更新日期:2012-11-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2014.34.3.223
更新日期:2014-05-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2013.33.4.248
更新日期:2013-07-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2017.37.6.516
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
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更新日期:2017-03-01 00:00:00
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更新日期:2017-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-09-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-07-01 00:00:00