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Establishing reference intervals for sex hormones on the analytical platforms Advia Centaur and Immulite 2000XP.

Abstract：

:Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 2000XP (Siemens Healthcare, Germany). We recruited healthy men (n=220), women in the follicular (n=139) or luteal (n=87) phases of the menstrual cycle, and postmenopausal women (n=103). Data was analyzed according to CLSI EP28-A3c guidelines. Although reference intervals established with both platforms showed good agreement with ranges quoted by the assay manufacturer, two discrepancies were noted. First, intervals for prolactin in women were influenced by hormonal status, and the partition analysis supported their separation into subgroups based on menstrual cycle. Second, the upper limit for estradiol in the follicular phase was nearly a half of that provided by the manufacturer. This discrepancy was attributed to the stringent definition of the follicular phase (consistently set at days 3-5 after menstruation onset). Our findings suggest that reference values for prolactin should both be gender specific and account for menstrual cycle phase. The results also emphasize that clear-cut selection criteria are required when assembling populations for establishing endocrine reference intervals.

journal_name

Ann Lab Med

journal_title

Annals of laboratory medicine

authors

Schüring AN,Kelsch R,Pierściński G,Nofer JR

doi

10.3343/alm.2016.36.1.55

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

55-9

issue

1

eissn

2234-3806

issn

2234-3814

pii

201601055

journal_volume

36

pub_type

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