Abstract:
BACKGROUND:Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. we developed QuantaMatrix Multiplexed Assay Platform (QMAP) MDR/XDR assay (QuantaMatrix Inc., Seoul, Korea) that can simultaneously detect mutations related to both first- and second-line drug resistance (rifampin, isoniazid, ethambutol, fluoroquinolones, second-line injectable drugs, and streptomycin). METHODS:We used 190 clinical Mycobacterium tuberculosis (MTB) strains isolated from Myanmar, compared QMAP and pDST results, and determined concordance rates. Additionally, we performed sequence analyses for discordant results. RESULTS:QMAP results were 87.9% (167/190) concordant with pDST results. In the 23 isolates with discordant results, the QMAP and DNA sequencing results completely matched. CONCLUSIONS:The QMAP MDR/XDR assay can detect all known DNA mutations associated with drug resistance for both MDR- and XDR-MTB strains. It can be used for molecular diagnosis of MDR- and XDR-TB to rapidly initiate appropriate anti-TB drug therapy.
journal_name
Ann Lab Medjournal_title
Annals of laboratory medicineauthors
Chang Y,Kim S,Kim Y,Ei PW,Hwang D,Lee J,Chang CL,Lee Hdoi
10.3343/alm.2020.40.2.142subject
Has Abstractpub_date
2020-03-01 00:00:00pages
142-147issue
2eissn
2234-3806issn
2234-3814pii
40.142journal_volume
40pub_type
杂志文章abstract:BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...
journal_title:Annals of laboratory medicine
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journal_title:Annals of laboratory medicine
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pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
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pub_type: 杂志文章
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journal_title:Annals of laboratory medicine
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journal_title:Annals of laboratory medicine
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journal_title:Annals of laboratory medicine
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