2,3,7,8-tetrachlorodibenzo-p-dioxin induced autophagy in a bovine kidney cell line.


:The administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to a variety of cultured cells may alter their ability to proliferate and die. In a previous study we demonstrated that TCDD induced proliferation in Madin-Darby Bovine Kidney (MDBK) cells where no signs of apoptosis were observed, but herein, analysis of MDBK cell morphology, in a large number of exposed cells, revealed some alterations, as expanded cytoplasm, an increase of intercellular spaces and many pyknotic nuclei. Hence, the aim of the current study was to elucidate the influences of dioxin on cell proliferation and cell death. We found that dioxin increased proliferation, as well as, activated cell death with autophagy, as we detected by increased amount of LC3-II, an autophagosome marker. Furthermore, formation of acidic vesicular organelles was observed by fluorescence microscopy following staining with the lysosomotropic agent acridine orange. These results were accompanied by down-regulation of telomerase activity, bTERT and c-Myc. Key tumor-suppressor protein p53 and expression of cell cycle inhibitor p21Waf1/Cip1 were activated after TCDD exposure. These changes occurred with activation of ATM phosphorylation in the presence of a decrease in Mdm2 protein levels. Taken together, these results support the idea that TCDD in MDBK cells, may exert its action, in part, by enhancing cell proliferation, but also by modulating the incidence of induced cell death with autophagy.






Fiorito F,Ciarcia R,Granato GE,Marfe G,Iovane V,Florio S,De Martino L,Pagnini U




Has Abstract


2011-12-18 00:00:00














  • Sub-acute toxicity of xylitol in rats; absence of hepatotoxicity.

    abstract::Some workers have suggested that 6 days xylitol administration to rats results in hepatic dysfunction. In our study xylitol was administered to rats by daily gastric intubation for a period of 14 days. Dose levels were 1.25, 2.5, 5 and 10 g/kg. During treatment (2, 5 and 14 days), animals were submitted to careful cli...


    pub_type: 杂志文章


    authors: Truhaut,Coquet B,Fouillet X,Galland L,Guyot D,Rouaud JL,Long DW

    更新日期:1977-08-01 00:00:00

  • Determination of mercury in fish from rivers and lakes in Hungary by atomic absorption technique.

    abstract::In 1975, in cooperation with 8 County Hygienic Stations, total mercury content in fish from rivers and lakes in Hungary was measured. The mercury content in fish muscle-tissue from 164 fish samples averaged 0.36 mg/kg. Average levels in different fish species ranged from 0.10 to 0.57 mg/kg. The amount of mercury in fi...


    pub_type: 杂志文章


    authors: Gergely A,Soós K,Erdélyi L,Cieleszky V

    更新日期:1977-06-01 00:00:00

  • Methylmercury promotes prostacyclin release from cultured human brain microvascular endothelial cells via induction of cyclooxygenase-2 through activation of the EGFR-p38 MAPK pathway by inhibiting protein tyrosine phosphatase 1B activity.

    abstract::Methylmercury is an environmental pollutant that exhibits neurotoxicity when ingested, primarily in the form of neuropathological lesions that localize along deep sulci and fissures, in addition to edematous and inflammatory changes in patient cerebrums. These conditions been known to give rise to a variety of ailment...


    pub_type: 杂志文章


    authors: Yoshida E,Kurita M,Eto K,Kumagai Y,Kaji T

    更新日期:2017-12-01 00:00:00

  • Influence of 2,3-dimercaptopropane-1-sulfonate and dimercaptosuccinic acid on the mobilization of mercury from tissues of rats pretreated with mercuric chloride, phenylmercury acetate or mercury vapors.

    abstract::The efficiency of the sodium salt of 2,3-dimercaptopropanesulfonic acid (DMPS) and meso-dimercaptosuccinic acid (DMSA) to mobilize mercury from tissues has been assessed in rats pretreated with different doses of HgCl2, phenylmercury acetate or exposed to different concentrations of mercury vapors. These pretreatments...


    pub_type: 杂志文章


    authors: Buchet JP,Lauwerys RR

    更新日期:1989-03-01 00:00:00

  • Histopathological and biochemical changes in the liver and testes of desert gerbil, after repeated exposures of Thimet (phorate).

    abstract::Intraperitoneal administration of Thimet (0.6 mg/kg) on alternate days for a period of 30 days produced various pathological and biochemical changes in the liver and testes of male gerbils. Histology of the liver showed necrosis, enlarged hepatocytes and fatty degeneration. Histological changes seen in the testes were...


    pub_type: 杂志文章


    authors: Saxena AK,Sarin K

    更新日期:1980-01-01 00:00:00

  • Inhibition of human erythrocyte Ca2+-ATPase by Zn2+.

    abstract::Recent investigations suggest that Ca2(+)-ATPase from fish gills is very sensitive to Zn2+ (Hogstrand et al., 1996. Am. J. Physiol. 270, R1141-R1147). The effect of free Zn2+ ion on the human erythrocyte plasma membrane Ca2(+)-ATPase was investigated to explore the possible extension of this finding to humans. Membran...


    pub_type: 杂志文章


    authors: Hogstrand C,Verbost PM,Wendelaar Bonga SE

    更新日期:1999-04-15 00:00:00

  • Increased carbon tetrachloride hepatotoxicity after low-level ethanol consumption.

    abstract::Male rats provided with a 5 or 15% (v/v) ethanol solution as the sole source of fluid consumed ethanol at a rate of 11.4 or 24.9% of total calories (4.2 or 8.3 g/kg daily). After ethanol consumption lasting 1, 2 and 3 weeks the hepatotoxicity of CCl4 (0.1 ml/kg i.p.) was elevated by determination of serum activities o...


    pub_type: 杂志文章


    authors: Strubelt O,Obermeier F,Siegers CP,Vöpel M

    更新日期:1978-07-01 00:00:00

  • Testosterone pretreatment mitigates cadmium toxicity in male C57 mice but not in C3H mice.

    abstract::Previous work has indicated that testosterone pretreatment protects against cadmium-induced toxicity in male rats while other data indicate that pretreatment of mice with testosterone offers no such protection against cadmium. Since cadmium toxicity may vary widely with species and strain, we examined the effect of te...


    pub_type: 杂志文章


    authors: Shimada H,Bare RM,Hochadel JF,Waalkes MP

    更新日期:1997-01-15 00:00:00

  • Inorganic tin -- a new selective inducer of the murine coumarin 7-hydroxylase (CYP2A5).

    abstract::The coumarin 7-hydroxylase of mice (Coh, CYP2A5) is known to be highly selectively inducible by both a set of heavy metals such as cobalt, indium and cerium and a variety of organic nitrogen-containing heteroaromatic compounds such as 3-amino-1,2,4-triazole, pyrazine and pyrazole. The investigations presented reveal t...


    pub_type: 杂志文章


    authors: Emde B,Tegtmeier M,Hahnemann B,Legrum W

    更新日期:1996-04-15 00:00:00

  • 109Cd accumulation in the calcified parts of rat bones.

    abstract::A recent epidemiological study showed an increased risk for bone fractures after chronic low-level cadmium exposure. This finding agrees with those of cadmium accumulation in rat bones after chronic oral exposure which reduced the mechanical strength of the bones. There are indications that ossicular cadmium uptake ma...


    pub_type: 杂志文章


    authors: Hunder G,Javdani J,Elsenhans B,Schümann K

    更新日期:2001-02-21 00:00:00

  • The acute vascular effects of cadmium in the testis do not require the presence of Leydig cells.

    abstract::In order to test the hypothesis that Leydig cells are required for the acute toxic effect of cadmium on the testis, adult male rats were treated with the Leydig cell toxin ethane dimethanesulphonate (EDS). Four days later, when all Leydig cells are removed from the testis, the EDS treated and control rats were given a...


    pub_type: 杂志文章


    authors: Bergh AR

    更新日期:1990-08-01 00:00:00

  • Olfactory and hepatic changes following inhalation of 3-trifluoromethyl pyridine in rats.

    abstract::Rats exposed by inhalation to 3-trifluoromethylpyridine (3-FMP) for 10, 30 or 90 days showed an unusual response in the nasal passages. Focal histological change including reduction in the number of cell layers, disorganisation, vacuolation and minimal necrosis was confined to the olfactory epithelium. Axon bundles an...


    pub_type: 杂志文章


    authors: Gaskell BA,Hext PM,Pigott GH,Hodge MC,Tinston DJ

    更新日期:1988-06-01 00:00:00

  • Cardio protective effect of glucose-insulin infusion on acute digoxin toxicity in rat.

    abstract:UNLABELLED:We recently observed a case of digoxin and insulin self-poisoning without cardiac repercussion. We raised the hypothesis that insulin may have a cardio-protective effect in case of digoxin toxicity. We have therefore evaluated the effect of glucose-insulin infusion on mortality and ECG abnormalities during a...


    pub_type: 杂志文章


    authors: Oubaassine R,Bilbault P,Roegel JC,Alexandre E,Sigrist S,Lavaux T,Jaeger A,Pinget M,Kessler L

    更新日期:2006-07-25 00:00:00

  • Cooperation of structurally different aryl hydrocarbon receptor agonists and β-catenin in the regulation of CYP1A expression.

    abstract::The ligand-activated nuclear receptor AhR (aryl hydrocarbon receptor) mediates the response of hepatocytes to various exogenous compounds. AhR is classically activated by planar, aromatic hydrocarbons, but also by other, structurally rather unrelated compounds. Recent data show that the canonical Wnt/β-catenin signali...


    pub_type: 杂志文章


    authors: Vaas S,Kreft L,Schwarz M,Braeuning A

    更新日期:2014-11-05 00:00:00

  • Chromosomal composition of micronuclei in mouse bone marrow treated with rifampicin and nicotine, analyzed by multicolor fluorescence in situ hybridization with pancentromeric DNA probe.

    abstract::The mechanism underlying the induction of micronuclei induced by rifampicin and nicotine in mouse bone marrow was investigated by fluorescence in situ hybridization assay using mouse minor satellite DNA probe. Colchicine and mitomycin, known to be predominantly clastogenic and aneugenic, respectively were used as posi...


    pub_type: 杂志文章


    authors: Attia SM

    更新日期:2007-06-03 00:00:00

  • Targeting specific cell signaling transduction pathways by dietary and medicinal phytochemicals in cancer chemoprevention.

    abstract::Natural phytochemicals derived from dietary sources or medicinal plants have gained significant recognition in the potential management of several human clinical conditions. Much research has also been geared towards the evaluation of plant extracts as effective prophylactic agents since they can act on specific and/o...


    pub_type: 杂志文章,评审


    authors: Neergheen VS,Bahorun T,Taylor EW,Jen LS,Aruoma OI

    更新日期:2010-12-05 00:00:00

  • Subacute toxicity studies with sodium saccharin and two hydrolytic derivatives.

    abstract::The subacute toxicity of sodium saccharin and 2 hydrolytic derivatives, o-sulfamoylbenzoic acid (Compound I) and ammonium o-carboxybenzene sulfonate (Compound II) was evaluated by feeding each of the compounds alone at a dietary level of 20 000 ppm to both beagle dogs and albino rats. Additionally, groups of dogs and ...


    pub_type: 杂志文章


    authors: Kennedy GL Jr,Fancher OE,Calandra JC

    更新日期:1976-08-01 00:00:00

  • Assessment of the sensitization potential of five metal salts in the murine local lymph node assay.

    abstract::The murine local lymph node assay (LLNA) has been proposed as a predictive test for the identification of sensitizing agents. We used this test to compare the sensitization potential of NiSO4, K2Cr2O7, CoCl2, Na2PtCl6 and BeSO4, salts of metals which have all been associated with allergic contact dermatitis and either...


    pub_type: 杂志文章


    authors: Mandervelt C,Clottens FL,Demedts M,Nemery B

    更新日期:1997-06-06 00:00:00

  • Fenvalerate inhibits the growth of primary cultured rat preantral ovarian follicles.

    abstract::Fenvalerate is a widely used synthetic pyrethroid insecticide and is reported to disrupt reproductive function in humans and animals. However, little is known about its influence on follicular development. In this study, rat preantral follicles were primary cultured to investigate the effects of fenvalerate on follicu...


    pub_type: 杂志文章


    authors: Fei J,Qu JH,Ding XL,Xue K,Lu CC,Chen JF,Song L,Xia YK,Wang SL,Wang XR

    更新日期:2010-01-12 00:00:00

  • Mutagenicity of 7-ketocholesterol in CHO cells: The role of lipid peroxidation.

    abstract::As an important cholesterol oxide, 7-ketocholesterol plays a deleterious role in the occurrence of cancer. Although the fact had been proved that 7-ketocholesterol could induce several biological phenomena, including apoptosis, DNA damage, et al., this issue whether 7-ketocholesterol led to mutagenesis in mammalian ce...


    pub_type: 杂志文章


    authors: Wang X,Li Y,Xia X,Zhang M,Ge C,Xia X,Xiao H,Xu S

    更新日期:2020-12-15 00:00:00

  • Lactational exposure to low levels of the six indicator non-dioxin-like polychlorinated biphenyls induces DNA damage and repression of neuronal activity, in juvenile male mice.

    abstract::Previously, we evaluated the effects of lactational exposure to a representative mixture of the six indicator non-dioxin-like polychlorinated biphenyls (∑6 NDL-PCBs) at low levels on the neurobiological changes and developmental/behavioral performances in mice. In this study, we analyzed the global gene expression pro...


    pub_type: 杂志文章


    authors: Elnar AA,Desor F,Marin F,Soulimani R,Nemos C

    更新日期:2015-02-03 00:00:00

  • Clostridium difficile toxin B inhibits the secretory response of human mast cell line-1 (HMC-1) cells stimulated with high free-Ca²⁺ and GTPγS.

    abstract::Clostridium difficile toxins A and B (TcdA and TcdB) belong to the class of large clostridial cytotoxins and inactivate by glucosylation some low molecular mass GTPases of the Rho-family (predominantly Rho, Rac and Cdc42), known as regulators of the actin cytoskeleton. TcdA and B also represent the main virulence fact...


    pub_type: 杂志文章


    authors: Balletta A,Lorenz D,Rummel A,Gerhard R,Bigalke H,Wegner F

    更新日期:2015-02-03 00:00:00

  • Effects of molybdate and pentachlorophenol on the sulfation of acetaminophen.

    abstract::Pentachlorophenol (PCP) is an inhibitor of phenol-sulfotransferases and has been used to ascertain the role of sulfation in toxicology. Recently, molybdate has been shown to inhibit the sulfation of various chemicals by decreasing hepatic concentrations of the cosubstrate, 3'-phosphoadenosine 5'-phosphosulfate (PAPS)....


    pub_type: 杂志文章


    authors: Boles JW,Klaassen CD

    更新日期:2000-04-20 00:00:00

  • Effects of o-aminoazotoluene on liver regeneration and p53 activation in mice susceptible and resistant to hepatocarcinogenesis.

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    pub_type: 杂志文章


    authors: Timofeeva OA,Eremeev AV,Goloshchapov A,Kalashnikova E,Ilnitskaya S,Setkov NA,Kobzev V,Buzard GS,Filipenko ML,Kaledin VI,Merkulova TI

    更新日期:2008-12-05 00:00:00

  • Application of a discovery to targeted LC-MS proteomics approach to identify deregulated proteins associated with idiosyncratic liver toxicity in a rat model of LPS/diclofenac co-administration.

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    pub_type: 杂志文章


    authors: Ramm S,Morissey B,Hernandez B,Rooney C,Pennington SR,Mally A

    更新日期:2015-05-04 00:00:00

  • Regulations on health/functional foods in Korea.

    abstract::The term "health/functional food" (HFF) refers to food supplements containing nutrients or other substances (in a concentrated form) that have a nutritional or physiological effect whose purpose is to supplement the normal diet. The Korean Health/Functional Food Act that came into effect in 2004 requires these product...


    pub_type: 杂志文章


    authors: Kim JY,Kim DB,Lee HJ

    更新日期:2006-04-03 00:00:00

  • Acute acrylonitrile exposure inhibits endogenous H2S biosynthesis in rat brain and liver: the role of CBS/3-MPST-H2S pathway in its astrocytic toxicity.

    abstract::Hydrogen sulfide (H2S) as the third gasotransmitter molecule serves various biological regulatory roles in health and disease. Acrylonitrile (AN) is a common occupational toxicant and environmental pollutant, causing brain and liver damage in mammals. The biotransformation of AN is dependent-upon reduced glutathione (...


    pub_type: 杂志文章


    authors: Yang B,Zhao W,Yin C,Bai Y,Wang S,Xing G,Li F,Bian J,Aschner M,Cai J,Shi H,Lu R

    更新日期:2021-01-21 00:00:00

  • Gut microbiota characterization and lipid metabolism disorder found in PCB77-treated female mice.

    abstract::Although the production of polychlorinated biphenyl 77 (PCB77) has already been banned globally, PCB77 is still used for a wide range of commercial purposes. Previous evidence has demonstrated that the PCB77 administration should be responsible for the gut microbiota variations and the host health risk. However, the h...


    pub_type: 杂志文章


    authors: Chi Y,Wang H,Lin Y,Lu Y,Huang Q,Ye G,Dong S

    更新日期:2019-05-15 00:00:00

  • Plasma concentration and placental transfer of bromofenofos in rats.

    abstract::Bromofenofos (BF) was administered by gavage once to non-pregnant and pregnant rats at 50 mg/kg and the plasma concentration-time curves of BF and its metabolite dephosphate bromofenofos (DBF) were determined by high-performance liquid chromatography. DBF reached a peak at 9 h, with 113.4 +/- 5.2 micrograms/ml, follow...


    pub_type: 杂志文章


    authors: Yoshimura H,Endoh YS

    更新日期:1989-05-15 00:00:00

  • Lead causes human fibroblasts to mis-sort arylsulfatase A.

    abstract::Lead exposure causes cognitive and behavioral deficits in some children. We have proposed that the effects of single nucleotide polymorphisms (SNP) of the human pseudodeficient arylsulfatase A (ARSA) gene that result in reduced levels of the enzyme, and lead concentrations that decrease ARSA activity, culminate in cel...


    pub_type: 杂志文章


    authors: Chen XG,Poretz RD

    更新日期:2001-06-21 00:00:00