Abstract：
:Arsenicals are known to induce ROS, which can lead to DNA damage, oxidative stress, and carcinogenesis. A human urothelial cell line, UROtsa, was used to study the effects of arsenicals on the human bladder. Arsenite [As(III)] and monomethylarsonous acid [MMA(III)] induce oxidative stress in UROtsa cells after exposure to concentrations as low as 1 microM and 50 nM, respectively. Previous research has implicated ROS as signaling molecules in the MAPK signaling pathway. As(III) and MMA(III) have been shown to increase phosphorylation of key proteins in the MAPK signaling cascade downstream of ErbB2. Both Src phosphorylation (p-Src) and cyclooxygenase-2 (COX-2) are induced after exposure to 50 nM MMA(III) and 1 microM As(III). These data suggest that ROS production is a plausible mechanism for the signaling alterations seen in UROtsa cells after acute arsenical exposure. To determine importance of ROS in the MAPK cascade and its downstream induction of p-Src and COX-2, specific ROS antioxidants (both enzymatic and non-enzymatic) were used concomitantly with arsenicals. COX-2 protein and mRNA was shown to be much more influenced by altering the levels of ROS in cells, particularly after MMA(III) treatment. The antioxidant enzyme superoxide dismutase (SOD) effectively blocked both As(III)-and MMA(III)- associated COX-2 induction. The generation of ROS and subsequent altered signaling did lead to changes in protein levels of SOD, which were detected after treatment with either 1 microM As(III) or 50 nM MMA(III). These data suggest that the generation of ROS by arsenicals may be a mechanism leading to the altered cellular signaling seen after low-level arsenical exposure.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Eblin KE,Hau AM,Jensen TJ,Futscher BW,Gandolfi AJdoi
10.1016/j.tox.2008.05.018subject
Has Abstractpub_date
2008-08-19 00:00:00pages
47-54issue
1eissn
0300-483Xissn
1879-3185pii
S0300-483X(08)00260-6journal_volume
250pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract：:p-Chloro-m-cresol (PCMC) and p-chloro-m-xylenol (PCMX) are known to cause allergic contact dermatitis. For risk assessment of skin sensitizers, information on dose-response profiles in the induction and elicitation phases and cross-reactivity with analogous chemicals are important. In the non-radioactive local lymph-n...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(03)00161-6
更新日期：2003-08-28 00:00:00
abstract：:Exposure to polycyclic aromatic hydrocarbons (PAHs) always involves complex mixtures that may induce synergistic or antagonistic effects on the genotoxic properties and make risk assessment more difficult. In this study, we evaluated how particulate PAHs modulated the formation of DNA damage induced by carcinogenic be...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2010.09.002
更新日期：2011-01-11 00:00:00
abstract：:Exposure of rats to 0.1 and 0.5 mg Cd/kg subcutaneously (s.c.) thrice weekly for 5 weeks resulted in an accumulation of cadmium in the liver in concentrations of 40 and 95 micrograms/g tissue, respectively, and a microsomal burden of Cd amounting to approx. 2-3% of the retained cadmium. The cytoplasm contained about 8...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(84)90079-9
更新日期：1984-09-14 00:00:00
abstract：:In the present study, we demonstrated the cytotoxic effect of a dimethylarsenic compound in seaweed, (R)-(2',3'-dihydroxypropyl) 5- deoxy-5-dimethylarsinoyl-beta-D-riboside, namely arsenosugar (AsSug), on mammalian cells, murine macrophages, in comparison with that of an inorganic arsenical, arsenite, in vitro. More t...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(97)00101-7
更新日期：1997-10-19 00:00:00
abstract：:Exposure to polychlorinated biphenyls (PCBs) is associated with numerous adverse health effects. Although the main route of exposure to PCBs is through the gastrointestinal tract, little is known about the contribution of the gut to the health effects of PCBs. We hypothesize that PCBs can disrupt intestinal integrity,...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2012.08.001
更新日期：2012-12-16 00:00:00
abstract：:Ethyl tertiary butyl ether (ETBE) is biofuel additive recently used in Japan and some other countries. Limited evidence shows that ETBE has low toxicity. Acetaldehyde (AA), however, as one primary metabolite of ETBE, is clearly genotoxic and has been considered to be a potential carcinogen. The aim of this study was t...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2013.06.005
更新日期：2013-09-15 00:00:00
abstract：:Five male and female rats per dose-group received 2,3,7,8-tetrabromodibenzo-p-dioxin (2,3,7,8-TBDD) once on the first day of the study. Doses of 10, 33, 100, or 300 micrograms 2,3,7,8-TBDD/kg body wt. and the vehicle control were administered by gavage. About 20% of 2,3,7,8-TBDD was excreted via feces. Severe body wei...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(92)90170-j
更新日期：1992-01-01 00:00:00
abstract：:Extracts from the leaves of Ginkgo biloba L. belong to the most widely used phytopharmaceuticals. In crude Ginkgo extracts, ginkgolic acids (GA) and related alkylphenols (e.g. cardanols and cardols) have been recognized as hazardous compounds with suspected cytotoxic, allergenic, mutagenic and carcinogenic properties....
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(02)00189-0
更新日期：2002-08-15 00:00:00
abstract：:Leflunomide, used for the treatment of rheumatoid arthritis, has been reported to cause severe liver problems and liver failure; however, the underlying mechanisms are not clear. In this study, we used multiple approaches including genomic analysis to investigate and characterize the possible molecular mechanisms of t...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2017.10.002
更新日期：2017-12-01 00:00:00
abstract：:Potassium dichromate was given to female Swiss mice (25 mg/kg per day) orally in water for 1-3 days. Brain homogenates were prepared to evaluate the occurrence of oxidative stress in this organ through the measurement of the antioxidant defense levels, and the extent of lipid peroxidation. In addition, mitochondrial f...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(00)00254-7
更新日期：2000-09-07 00:00:00
abstract：:Trichloroacetic acid, TCA, is a water chlorination by-product similar to dichloroacetic acid, DCA. Because DCA has been shown to have effects on intermediary metabolism, TCA was tested to determine if it possesses similar capabilities. The effects were more pronounced in females. High doses of TCA (2.45 mumol/kg three...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(90)90069-s
更新日期：1990-07-01 00:00:00
abstract：:The effects of N-acetylcysteine (NAC), 2-mercaptopropionylglycine (MPG) and dithiothreitol (DTT) on the metabolism and toxicity of acetaminophen (APAP) were examined in isolated mouse hepatocytes maintained in primary culture on collagen-coated dishes. Both NAC and MPG increased the formation of the glutathione and su...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(86)90106-x
更新日期：1986-10-01 00:00:00
abstract：:Arsenic and cadmium are both priority hazardous substances and human carcinogens. Although there is the potential for simultaneous exposure to both metals, the interactions of cadmium and arsenic are not well defined. We examined the toxicity of these metals when given alone or in alternating sequence to adult male Fi...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(96)03536-6
更新日期：1997-01-15 00:00:00
abstract：:The cytotoxicity and cardiotoxicity of benzoyl mustard (FCE 24517) and epoxamido (FCE 24561) synthetic derivatives of distamycin A were reported in the present study. The 50% inhibiting concentration (IC50) of colony formation of FCE 24517 on human SNB-19 glioblastoma, A2780 ovarian cancer and DU 145 prostate cancer w...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(92)90003-w
更新日期：1992-11-15 00:00:00
abstract：:Beside heavy metals, cadmium (Cd(2+)) is a ubiquitous toxic metal with a well established apoptotic and genotoxic effect, chronic exposure of which has been involved in a variety of pathological conditions. In the present study, we investigated by 1455 genes cDNA microarrays the toxic and apoptotic effect of Cd(2+), o...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(02)00236-6
更新日期：2002-09-02 00:00:00
abstract：:Between the DNA sequences of two randomly-selected human genomes, which consist of over 3 billion base pairs and twenty five thousand genes, there exists only 0.1% variation and 99.9% sequence identity. During the last couple of decades, extensive genome-wide studies have investigated the association between single-nu...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/j.tox.2017.03.020
更新日期：2017-05-15 00:00:00
abstract：:Gambogic acid (GA), a major active component of gamboge, exhibits potent anticancer activity in many kinds of cancer cells. However, the anticancer mechanism of GA is not clearly understood. Here we showed that GA could cause growth inhibition, induce the G0/G1 phase cell cycle arrest and apoptosis in human chronic my...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2009.04.059
更新日期：2009-08-03 00:00:00
abstract：:Troglitazone is a thiazolidinedione antidiabetic agent with insulin-sensitizing activities that was withdrawn from the market in 2000 due to its association with idiosyncratic hepatotoxicity. To address the suspected autoantibody production associated with troglitazone, we investigated autoantibodies in sera from pati...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2005.07.012
更新日期：2005-12-01 00:00:00
abstract：:Polycyclic aromatic hydrocarbons (PAHs) including benzo[a]pyrene (B[a]P) and 7,12-dimethylbenz[a]anthracene (DMBA) are environmental pollutants, which undergo metabolic activation to exert their carcinogenic effects. Our earlier studies showed that naturally occurring plant phenols, protocatechuic, chlorogenic, tannic...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2008.07.047
更新日期：2008-09-29 00:00:00
abstract：:Although it is well known that microcystin-LR (MC-LR) may cause male reproductive toxicity, less is known on its potential impact on the development of prostate. In this study, from the 12th day in the embryonic period to the 21st day after birth, 4 randomly assigned groups of pregnant mice were exposed to 0, 1, 10, a...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2017.02.004
更新日期：2017-04-01 00:00:00
abstract：:Mitochondrial fission, fusion, and mitophagy are interlinked processes that regulate mitochondrial shape, number, and size, as well as metabolic activity and stress response. The fundamental importance of these processes is evident in the fact that mutations in fission (DRP1), fusion (MFN2, OPA1), and mitophagy (PINK1...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2017.05.018
更新日期：2017-07-15 00:00:00
abstract：:Bisphenol A has been shown to affect the reproduction of male rats and mice. However, the mechanism of action of bisphenol A on the epididymal sperm is not elucidated. The present study was undertaken to evaluate the effect of bisphenol A on the antioxidant system of rat epididymal sperm. Bisphenol A was administered ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(02)00597-8
更新日期：2003-03-14 00:00:00
abstract：:D-Serine causes selective necrosis to the straight portion of the rat renal proximal tubules. The onset is rapid, occurring within 3-4 h and accompanied by proteinuria, glucosuria and aminoaciduria. The metabolism of D-serine by D-amino acid oxidase (D-AAO) may be involved in the mechanism of toxicity. D-AAO is locali...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.08.008
更新日期：2005-02-01 00:00:00
abstract：:The developing nervous system is susceptible to lead (Pb) exposure but less is known about the effect of this toxic agent in adult rat brain. Since astrocytes serve as a cellular Pb deposition site, it is of importance to investigate the response of astroglial cells in the adult rat brain in a model of acute lead expo...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(01)00415-2
更新日期：2001-08-28 00:00:00
abstract：:Induction of hepatic metallothionein (MT) by acetaminophen was characterized in the rat and mouse. Treatment of rats with the hepatotoxin resulted in increase of liver MT in a dose-dependent manner. MT concentration was elevated by 41%, 140% and 260% following acetaminophen injection at doses of 250, 500 and 1000 mg/k...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(88)90224-7
更新日期：1988-12-30 00:00:00
abstract：:The fetotoxicity of nitrofen and 5 analogous diphenyl ethers was compared in mice, using survival on postnatal day 5 to evaluate prenatal and perinatal toxicity. The effect of each chemical on the Harderian gland weight of surviving pups was also assessed, since decreased Harderian gland weight is seen in nitrofen-exp...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(86)90062-4
更新日期：1986-09-01 00:00:00
abstract：:Both catecholamine (CA) released from adrenal chromaffin cells and hydrogen sulfide (H2S) have been shown to play critical roles in the regulation of hypoxic stress response. Our previous study has demonstrated that exogenous H2S directly induced quantal CA released from adult rat adrenal chromaffin cells (ARACCs) by ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2013.06.014
更新日期：2013-10-04 00:00:00
abstract：:This paper introduces the special issue on quantitative in vitro-in vivo extrapolations (QIVIVE). It highlights important issues in the development of in vitro toxicology towards its implementation in toxicological risk assessment. ...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/j.tox.2014.04.004
更新日期：2015-06-05 00:00:00
abstract：:In the rat the hepatotoxicity of bromobenzene is greatly enhanced by the introduction of a cyano group adjacent to the bromine (i.e. o-bromobenzonitrile). Epoxide metabolites of these aryl halides are believed to be the actual toxic species, but epoxides of the latter compound also possess a second site of chemical re...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(84)90106-9
更新日期：1984-06-01 00:00:00
abstract：:It is accepted practice in presenting organ weight data to express the results relative to the animal's body weight. The data presented suggest that in rats no benefits are obtained from this method either in reducing the variability of the data or in removing bias due to different body weights. By using simulated dat...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(76)90050-0
更新日期：1976-03-01 00:00:00
