Abstract:
:The effects of N-acetylcysteine (NAC), 2-mercaptopropionylglycine (MPG) and dithiothreitol (DTT) on the metabolism and toxicity of acetaminophen (APAP) were examined in isolated mouse hepatocytes maintained in primary culture on collagen-coated dishes. Both NAC and MPG increased the formation of the glutathione and sulfate conjugates of APAP and decreased the covalent binding of the APAP reactive metabolite to cellular protein. DTT did not increase APAP metabolism but did decrease covalent binding. NAC, MPG and DTT decreased plasma membrane damage, as measured by leakage of lactate dehydrogenase from hepatocytes, during a 4-h incubation in 5.0 mM APAP. NAC, MPG and DTT also reduced the APAP-induced fall in glutathione levels in these cells. In other experiments, hepatocytes were exposed to 5.0 mM APAP for 1 h and then incubated during a post-exposure period in APAP-free medium. Damage increased during this post-exposure incubation. Addition of DTT, but not NAC or MPG, after APAP exposure protected the hepatocytes from plasma membrane damage during the post-exposure period. These results indicate that NAC and MPG exert their protective effects by their action on the reactive metabolite of APAP. As well as its effect in reducing the formation of the reactive metabolite, DTT has a potent protective effect against the toxic processes initiated by the APAP reactive metabolite.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Harman AW,Self Gdoi
10.1016/0300-483x(86)90106-xsubject
Has Abstractpub_date
1986-10-01 00:00:00pages
83-93issue
1eissn
0300-483Xissn
1879-3185pii
0300-483X(86)90106-Xjournal_volume
41pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract::There has been an intensive debate whether dietary exposure to acrylamide could increase the risk of human cancer since the first description of the presence of acrylamide in food in 2002. As the intestinal mechanisms of acrylamide absorption are poorly investigated we studied the transport of acrylamide in differenti...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.12.014
更新日期:2007-03-22 00:00:00
abstract::High-throughput in vitro toxicity screening provides an efficient way to identify potential biological targets for environmental and industrial chemicals while conserving limited testing resources. However, reliance on the nominal chemical concentrations in these in vitro assays as an indicator of bioactivity may misr...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2014.05.012
更新日期:2015-06-05 00:00:00
abstract::For half of the 30-odd years that diacetyl-exposed workers have developed disabling lung disease, obliterative bronchiolitis was unrecognized as an occupational risk. Delays in its recognition as an occupational lung disease are attributable to the absence of a work-related temporal pattern of symptoms; failure to rec...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2016.06.009
更新日期:2017-08-01 00:00:00
abstract::The use of colchicine-specific Fab fragments is of interest in human poisoning. In the present study, we show the efficacy of Fab fragments in reversing colchicine toxicity in mice. High affinity antibodies (Ka = 2 x 10(10) M-1) against colchicine were raised in goats; Fab fragments were purified by DEAE chromatograph...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(91)90015-s
更新日期:1991-01-01 00:00:00
abstract::The attempts to use nitroxide free radicals and nitrone spin traps topically in skin requires analysis of their potential cutaneous adverse effects. The objective of this study was to investigate the skin irritation and sensitizing potential of nitroxides and nitrones in the guinea pig. The following unsubstituted nit...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(97)00183-2
更新日期:1998-02-20 00:00:00
abstract::Trans-1,2-dichloroethylene (t-DCE), an industrial solvent, proved to be moderately toxic when studied in small laboratory animals. In adult female rats brief (8 h) and prolonged (8 h daily, on 5 consecutive days a week, for more than 16 weeks) inhalation of 200 ppm--the current TLV/MAC in various countries--produced h...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(77)90059-2
更新日期:1977-04-01 00:00:00
abstract::The nitric oxide (NO) donor, O(2)-vinyl 1-(pyrrolidin-1-yl)diazen-1-ium-1,2-diolate (V-PYRRO/NO), is metabolized by P450 enzymes to release NO in the liver and possibly other tissues. V-PYRRO/NO has been shown to be hepatoprotective, but little is known about its effect in the kidney, another organ rich in P450s. Thus...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(03)00129-x
更新日期:2003-08-01 00:00:00
abstract::Ochratoxin A (OTA) is a mycotoxin produced by several fungi which grow on human food source material. Consumption of OTA is almost unavoidable. The consumption leads to low but detectable amounts of OTA in human blood. Risk assessment of OTA is based on studies performed either in animals or cultured cells. So far, ma...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.12.008
更新日期:2007-03-22 00:00:00
abstract::In vitro stimulation of bronchoalveolar lavage cells from patients with chronic beryllium disease (CBD) induces the production of TNF-alpha. We tested the hypothesis that beryllium (Be)-stimulated TNF-alpha might induce apoptosis in mouse and human macrophage cell lines. These cell lines were selected because they pro...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(00)00237-7
更新日期:2000-08-21 00:00:00
abstract::To evaluate hydroperoxide generation as a potential mechanism of cyanide neurotoxicity, mice were treated with KCN (7 mg/kg, subcutaneously (s.c.)) and the level of lipid peroxidation (expressed as conjugated dienes) was measured later in various organs. Brain showed elevated conjugated diene levels after cyanide but ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(94)90221-6
更新日期:1994-04-18 00:00:00
abstract::In the clinical setting, antidotes are generally administered after the occurrence of a drug overdose. Therefore, the most pertinent evaluation of any new agent should model human exposure. This study tested whether acetaminophen (APAP) hepatotoxicity was reversed when S-adenosyl-L-methionine (SAMe) was administered a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2007.10.027
更新日期:2008-02-03 00:00:00
abstract::Technical pentabrominated diphenyl ether (pentaBDE mix) is a mixture of polybrominated diphenyl ethers (PBDEs) which has been widely used as a flame retardant. Since its ban in several countries it has been replaced by other brominated flame retardants such as hexabromocyclododecane (HBCD). Both certain PBDE congeners...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.07.009
更新日期:2009-10-01 00:00:00
abstract::Although it is well known that microcystin-LR (MC-LR) may cause male reproductive toxicity, less is known on its potential impact on the development of prostate. In this study, from the 12th day in the embryonic period to the 21st day after birth, 4 randomly assigned groups of pregnant mice were exposed to 0, 1, 10, a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2017.02.004
更新日期:2017-04-01 00:00:00
abstract::Recently, a large-scale field study in The Netherlands has focused on the effects of estrogenic contaminants on feral fish populations. The freshwater bream (Abramis brama) and the estuarine flounder (Platichthys flesus) were sampled at a large number of locations in the spring and autumn of 1999. Concentrations of th...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00271-8
更新日期:2002-12-27 00:00:00
abstract::The 4 S polycyclic aromatic hydrocarbon (PAH)-binding protein had been implicated in regulating the expression of rat cytochrome P450IA1 which is most closely associated with aryl hydrocarbon hydroxylase (AHH). We have now investigated the presence of both the 4 S PAH-binding protein and the 8 S Ah receptor in rat hep...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(91)90217-o
更新日期:1991-02-01 00:00:00
abstract::The early neonatal stage constitutes a sensitive period during which exposure to adverse events can increase the risk of neuropsychiatric disorders. Maternal deprivation (MD) is a model of early life stress that induces long-term behavioural and physiological alterations, including susceptibility to different drugs of...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.12.003
更新日期:2013-09-06 00:00:00
abstract::To further investigate the immunotoxic effects of tri-n-propyltin chloride (TPTC), tri-n-butyltin chloride (TBTC) and triphenyltin chloride (TPhTC) several cytotoxicity tests with a series of trialkyltin chlorides and TPhTC were carried out, using isolated rat thymocytes as target cells. Thymocytes, cultured in a seru...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(86)90160-5
更新日期:1986-04-01 00:00:00
abstract::Tobacco smoking is the largest preventable risk factor for morbidity and mortality in industrialized countries. WHO estimates that tobacco will become the largest single health problem by 2020, causing an estimated 8.4 million deaths annually. Tobacco has central importance in the etiology of cancers of the lung, head...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/s0300-483x(01)00445-0
更新日期:2001-09-14 00:00:00
abstract::The effects of ethanol on hamster hepatic and extrahepatic monooxygenases were determined in the present study. Chronic ethanol administration increased cytochrome P-450 (P-450) content and monooxygenase activities towards aniline, N-nitrosodimethylamine, and 7-ethoxyresorufin. In contrast, benzphetamine and benzo(a)p...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(93)90006-e
更新日期:1993-07-28 00:00:00
abstract::The long-term clinical usefulness of doxorubicin (DOX), an anthracycline with potent antitumor activity, is limited by DOX-induced cardiotoxicity. Kaempferol, one of the most common dietary flavonoids, is known to have anti-apoptotic, anti-oxidative, and anti-inflammatory properties. The current study aimed to investi...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.11.018
更新日期:2012-02-06 00:00:00
abstract::It is reported that gambogic acid (GA), the main active compound of gamboge which is a dry resin extracted from Garcinia hanburyi tree, has potent antitumor activity both in vivo and in vitro. Activation of mitochondrial apoptotic pathway in cancer cells is one effective therapy for cancer treatment. In the present st...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.03.010
更新日期:2009-06-16 00:00:00
abstract::To investigate mechanisms of chemical-induced congenital heart defects (CHD) we have developed a rat model using dimethadione (DMO), the N-demethylated metabolite of the anticonvulsant, trimethadione (TMD). Dosing pregnant rats with 300mg/kg DMO every 12h from the evening of gestational day (GD) 8 until the morning of...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2014.10.014
更新日期:2014-12-04 00:00:00
abstract::Silver nanoparticles (AgNPs) are widely utilized in various consumer products and medical devices, especially due to their antimicrobial properties. However, several studies have associated these particles with toxic effects, such as inflammation and oxidative stress in vivo and cytotoxic and genotoxic effects in vitr...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.09.014
更新日期:2013-11-08 00:00:00
abstract::To investigate the biological effects of terbium (Tb), male mice were intraperitoneally administered with TbCl3 at doses of 10, 50, or 250 mg Tb/kg. The Tb distribution in organs was determined after 18-20 h of injection by using a spectrofluorometer. The concentrations of Ca, Mg, Fe, and Zn in various organs were det...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(91)90181-y
更新日期:1991-02-11 00:00:00
abstract::Aluminum (Al) is recognized worldwide as serious inorganic contaminants. Exposure to Al is associated with low BMD and an increased risk of osteoporosis. However, the precise molecular mechanisms remains unclear. Thus, in this study, rats were orally exposed to 0 (control group, CG) and 0.4g/L AlCl3 (AlCl3 treated gro...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2016.10.002
更新日期:2016-09-14 00:00:00
abstract::The consumption of diets rich in plant foods are associated with a reduced risk of cardiovascular diseases. This study was aimed to evaluate the role of S-allylcysteine (SAC) in isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (150 mg/kg) to Wistar rats showed a significant...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.04.039
更新日期:2006-07-05 00:00:00
abstract::The objective of this study was to establish a hypospadiac rat model by maternal exposure to di-n-butyl phthalate (DBP) and to evaluate the developmental abnormalities of hypospadiac male rats. Timed-pregnant rats were given DBP by gastric intubation at doses of 0, 250, 500, 750 or 1000 mg/kg body weight (bw)/day from...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2007.01.018
更新日期:2007-04-11 00:00:00
abstract::Mitochondria are cellular powerhouses as well as metabolic and signaling hubs regulating diverse cellular functions, from basic physiology to phenotypic fate determination. It is widely accepted that reactive oxygen species (ROS) generated in mitochondria participate in the regulation of cellular signaling, and that s...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2017.07.013
更新日期:2017-11-01 00:00:00
abstract::The allergenicity of various compounds was tested by means of the optimization procedure in the guinea-pig. Results with antibiotics, preservatives, fragrance raw materials and miscellaneous contactants are reported. Their relevance is critically discussed in relation to other animal sensitization methods (Draize, Büh...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(80)90050-5
更新日期:1980-01-01 00:00:00
abstract::Dietary exposure to vomitoxin (VT) results in hyperelevated serum IgA and IgA nephropathy in mice. To assess the possible role of cytokines in this IgA dysregulation, the effects of a single oral exposure in B6C3F1 male mice to 0, 5 or 25 mg/kg BW VT on production of IgA and cytokines in Peyer's patch (PP) and spleen ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(97)00087-5
更新日期:1997-09-26 00:00:00