Abstract:
:In vitro stimulation of bronchoalveolar lavage cells from patients with chronic beryllium disease (CBD) induces the production of TNF-alpha. We tested the hypothesis that beryllium (Be)-stimulated TNF-alpha might induce apoptosis in mouse and human macrophage cell lines. These cell lines were selected because they produce a range of Be-stimulated TNF-alpha. The mouse macrophage cell line H36.12j produces high levels of Be-stimulated TNF-alpha. The mouse macrophage cell line P388D.1 produces low, constitutive, levels of TNF-alpha and does not up-regulate Be-stimulated TNF-alpha production. The DEOHS-1 human CBD macrophage cell line does not produce constitutive or Be-stimulated TNF-alpha. Apoptosis was determined by microscopic observation of propidium iodide stained fragmented nuclei in unstimulated and BeSO(4)-stimulated macrophage cell lines. BeSO(4) induced apoptosis in all macrophage cell lines tested. Beryllium-stimulated apoptosis was dose-responsive and maximal after 24 h of exposure to 100 microM BeSO(4). In contrast, unstimulated and Al(2)(SO(4))(3)-stimulated macrophage cell lines did not undergo apoptosis. The general caspase inhibitor BD-fmk inhibited Be-stimulated macrophage cell line apoptosis at concentrations above 50 microM. Our data show that Be-stimulated macrophage cell line apoptosis was caspase-dependent and not solely dependent on Be-stimulated TNF-alpha levels. We speculate that the release of Be-antigen from apoptotic macrophages may serve to re-introduce Be material back into the lung microenvironment, make it available for uptake by new macrophages, and thereby amplify Be-stimulated cytokine production, promoting ongoing inflammation and granuloma maintenance in CBD.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Sawyer RT,Fadok VA,Kittle LA,Maier LA,Newman LSdoi
10.1016/s0300-483x(00)00237-7keywords:
subject
Has Abstractpub_date
2000-08-21 00:00:00pages
129-42issue
2-3eissn
0300-483Xissn
1879-3185pii
S0300483X00002377journal_volume
149pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract::The Comet assay is an often used approach for the assessment of genetic damage in primary cells of exposed populations. In the majority of these studies lymphocytes are used. Therefore, we reviewed human biomonitoring studies of occupational exposure using the Comet assay with lymphocytes. We also tried to elucidate t...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2004.02.010
更新日期:2004-05-20 00:00:00
abstract::In a Japanese chemical factory, a lung disease like pneumoconiosis appeared at a high rate among workers handling cross-linked water-soluble acrylic acid polymer (CWAAP). To our knowledge, no such case was known in the world until very recently. The present study was designed to elucidate the effect of single intratra...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2020.152647
更新日期:2021-01-30 00:00:00
abstract::There has been an intensive debate whether dietary exposure to acrylamide could increase the risk of human cancer since the first description of the presence of acrylamide in food in 2002. As the intestinal mechanisms of acrylamide absorption are poorly investigated we studied the transport of acrylamide in differenti...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.12.014
更新日期:2007-03-22 00:00:00
abstract::Nitrated-polycyclic aromatic hydrocarbons (nitro-PAHs) and diesel exhaust particle extracts (DEPE) induced apoptosis in Hepa1c1c7 cells with the following potency: 1,3-dinitropyrene (1,3-DNP)>1-nitropyrene (1-NP) > DEPE > 1,8-dinitropyrene (1,8-DNP). The compounds induced cyp1a1, and activated various intracellular si...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.12.009
更新日期:2007-03-07 00:00:00
abstract::Gordonia axillaris (Roxb.) Dietrich (Theaceae) is a native to Taiwan and the leaves have been used as an astringent folk medicine. Camelliin B (CB), a macrocyclic hydrolyzable tannin, was isolated from G. axillaris and showed cytotoxic effects in human carcinoma cells. Among the target cells (SKHep-1, Ha-22T, DU-145, ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(01)00452-8
更新日期:2001-11-30 00:00:00
abstract::The use of colchicine-specific Fab fragments is of interest in human poisoning. In the present study, we show the efficacy of Fab fragments in reversing colchicine toxicity in mice. High affinity antibodies (Ka = 2 x 10(10) M-1) against colchicine were raised in goats; Fab fragments were purified by DEAE chromatograph...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(91)90015-s
更新日期:1991-01-01 00:00:00
abstract::Asthma, which is primarily an allergic type of respiratory disease, has increased in the U.S. and Europe by 30% over the last decade. Air pollution may play a role in this rise, since during episodes of smog, hospital admissions due to asthma increase. Ambient air quality has generally improved since the Clean Air Act...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/0300-483x(95)03230-d
更新日期:1995-12-28 00:00:00
abstract::The aim of this study was to compare the roles of dihydropyridine calcium antagonists nifedipine, nitrendipine, amlodipine on doxorubicin (DXR)-induced nephrotoxicity in rats using biochemical, histopathological and immunohistochemical approaches. Male Sprague-Dawley rats were randomly divided into five experimental g...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.11.067
更新日期:2007-02-28 00:00:00
abstract::Defining the impact on health of exposure to a low-dose pesticide mixture via food intake is a topical question since epidemiological studies suggest that this may increase the risk of pathologies and particularly haematopoietic malignancies. Here we investigated on the haematopoietic system of mice, the effect of a m...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.10.024
更新日期:2010-01-12 00:00:00
abstract::Pregnant CD-1 mice were administered a commercial 2,4-dichlorophenoxyacetic acid (2,4-D) formulation on days 6-16 days of gestation, in drinking water at concentrations ranging from 0 to 1.0% of the formulated product, equivalent to approximately 0-650 mg/kg per day expressed as the amine derivative. The effect of 2,4...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(01)00403-6
更新日期:2001-08-13 00:00:00
abstract::Role of chromium(III) in biological systems is controversial and no significant conclusions can be arrived with the available data. Chromium(VI) enters in to the cell readily and gets reduced to form stable chromium(III) complexes. Chromium(III) complexes react slowly and were considered non-toxic. Recent studies sugg...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2008.07.052
更新日期:2008-09-29 00:00:00
abstract::Phensuximide (PSX) is an antiepileptic agent which has been shown to induce hemorrhagic cystitis and mild nephrotoxicity following repeated administration in man or rats or when acutely administered to phenobarbital-pretreated rats. The purpose of this study was to explore the role of para-hydroxylation of the phenyl ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(92)90045-g
更新日期:1992-08-01 00:00:00
abstract::Several dicationic amphiphilic compounds, such as the immunomodulator tilorone and analogues, impair the lysosomal catabolism of sulphated glycosaminoglycans (GAGs). Thereby they cause lysosomal GAG storage in rats and in cultured fibroblasts of several species including man. The GAG storage is rather slowly reversibl...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(98)00052-3
更新日期:1998-07-03 00:00:00
abstract::The induction of unscheduled DNA synthesis (UDS) in early spermatid stages of mice was studied after treatment with hydrazine or procarbazine (Natulan). The UDS response was assayed by measuring the levels of [3H]dThd in the nuclei of sperm cells derived from treated and untreated early spermatid stages. The radioacti...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(82)90030-0
更新日期:1982-01-01 00:00:00
abstract::Paracetamol, in toxic doses, is associated with extensive liver damage. This represents one of the common causes of morbidity and mortality in drug poisoning cases. This study was undertaken to investigate the possible potentiation of the hepatoprotective action of N-acetylcysteine (NAC) by cimetidine (CMD), an inhibi...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(97)00069-3
更新日期:1997-09-05 00:00:00
abstract::Proliferation and differentiation of cells in female reproductive organs, the oviduct, uterus and vagina, are regulated by endogenous estrogen. In utero exposure to a synthetic estrogen, diethylstilbestrol (DES), induces vaginal clear-cell adenocarcinoma in humans. In mice, perinatal exposure to DES results in abnorma...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.02.010
更新日期:2012-06-14 00:00:00
abstract::We recently reported that Delta(9)-tetrahydrocannabinol (Delta(9)-THC) has the ability to stimulate the proliferation of human breast carcinoma MCF-7 cells. However, the mechanism of action remains to be clarified. The present study focused on the relationship between receptor expression and the effects of Delta(9)-TH...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2007.12.019
更新日期:2008-03-12 00:00:00
abstract::Cytochrome P450 (CYP) 2A13 is mainly expressed in the respiratory system and has the ability to metabolize aflatoxin B(1) (AFB(1)). However, the role of CYP2A13-mediated AFB(1) metabolism and its consequences in human lung epithelial cell is not clear. Therefore, the objectives of this study were to investigate the si...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.06.010
更新日期:2012-10-28 00:00:00
abstract::Several epidemiological studies have shown that acute exposure to particulate air pollution is associated with increases in cardiovascular morbidity and mortality, and that these effects are especially exacerbated among individuals with pre-existing compromised cardiovascular function such as hypertension. This study ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.03.018
更新日期:2011-07-11 00:00:00
abstract::The concept that the highly reactive hydroxyl radical (HO) could be generated from an interaction between superoxide (O(2)(-)) and hydrogen peroxide (H(2)O(2)) was proposed (with Joseph Weiss) in Professor Haber's final paper published in 1934. Until it was recognized that free radicals are produced in biological syst...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/s0300-483x(00)00231-6
更新日期:2000-08-14 00:00:00
abstract::Preclinical drug safety assessment includes in vitro studies with physiologically relevant cell cultures. As an in vitro system for hepatic toxicology testing, we have been generating cell clones of human hepatoblastoma cell line HepG2 by lentiviral transduction of phase I cytochrome P450 (CYP) enzymes. Here, we prese...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2018.11.008
更新日期:2019-01-15 00:00:00
abstract::Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), a major constituent of marijuana, has been shown to stimulate the growth of MCF-7 breast cancer cells through cannabinoid receptor-independent signaling [Takeda, S., Yamaori, S., Motoya, E., Matsunaga, T., Kimura, T., Yamamoto, I., Watanabe, K., 2008. Delta(9)-Tetrahydroca...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.01.024
更新日期:2009-05-02 00:00:00
abstract::The early neonatal stage constitutes a sensitive period during which exposure to adverse events can increase the risk of neuropsychiatric disorders. Maternal deprivation (MD) is a model of early life stress that induces long-term behavioural and physiological alterations, including susceptibility to different drugs of...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.12.003
更新日期:2013-09-06 00:00:00
abstract::Human liver microsomes, supersomes from baculovirus-transformed insect cells expressing different human CYP450 isoforms, and control and CYP3A4 cDNA-transfected V79 Chinese hamster cells were tested for their ability to metabolize territrem B (TRB) and territrem C (TRC). Two TRB metabolites, designated MB(2) and MB(4)...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2005.10.010
更新日期:2006-02-01 00:00:00
abstract::Ochratoxin A (OCT A) has the potential to cause myelotoxicity in addition to the well-known toxic effects on the liver and kidney. Whereas in previous studies the bone marrow parameters were examined shortly after injection, experiments reported here were designed to determine whether mice would recover from the myelo...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(88)90236-3
更新日期:1988-12-16 00:00:00
abstract::Conditions for the optimal expression of the human CYP1B1 hemoprotein in Escherichia coli have been investigated. CYP1B1 cDNA was prepared from a retinal cDNA template and used to generate cDNA fragments with modified 5'-sequences reported to allow enhanced expression in E. coli DH5alpha. Plasmids were constructed, us...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(99)00209-7
更新日期:2000-04-03 00:00:00
abstract::For quantitative in vitro-in vivo extrapolation (QIVIVE) of metabolism for the purposes of toxicokinetics prediction, a precise and robust analytical technique for identifying and measuring a chemical and its metabolites is an absolute prerequisite. Currently, high-resolution mass spectrometry (HR-MS) is a tool of cho...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2013.08.010
更新日期:2015-06-05 00:00:00
abstract::Palmitoylpentachlorophenol (PPCP), which is a lipid conjugate of a xenobiotic compound, has been found in human fat. To study the toxicity associated with PPCP, rats were given 100 mg/kg PPCP and sacrificed at 4, 8 and 12 days. The target organ identified was the exocrine pancreas; no other major organs examined showe...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(87)90137-5
更新日期:1987-10-12 00:00:00
abstract::Groundwater (GW) from the Bitterfeld industrial region, Central Germany, is contaminated mainly with monochlorobenzene (MCB). Accordingly, current research addresses the development of feasible in situ groundwater remediation technologies. Although easily degradable under aerobic conditions, MCB persists in the essent...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2004.06.052
更新日期:2004-12-15 00:00:00
abstract::The carcinogenic response of NZO/BlGd inbred mice of both sexes to oral N-nitrosohexamethyleneimine (NHEX) was investigated at 2 differing dose rates, but equal cumulative doses. Mice received 52 mg NHEX (2 mg/g body wt) in drinking water over either an 8-week period (200 mg/l) or a 32-week period (50 mg/l), and were ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(84)90042-8
更新日期:1984-12-01 00:00:00