Abstract:
:Several isolates of Bacillus thuringiensis (Bt) were screened for the vegetative insecticidal protein (Vip) effective against sap-sucking insect pests. Screening results were based on LC(50) values against cotton aphid (Aphis gossypii), one of the dangerous pests of various crop plants including cotton. Among the isolates, the Bt#BREF24 showed promising results, and upon purification the aphidicidal protein was recognized as a binary toxin. One of the components of this binary toxin was identified by peptide sequencing to be a homolog of Vip2A that has been reported previously in other Bacillus spp. Vip2 belongs to the binary toxin group Vip1-Vip2, and is responsible for the enzymatic activity; and Vip1 is the translocation and receptor binding protein. The two genes encoding the corresponding proteins of the binary toxin, designated as vip2Ae and vip1Ae, were cloned from the Bt#BREF24, sequenced, and heterologously expressed in Escherichia coli. Aphid feeding assay with the recombinant proteins confirmed that these proteins are indeed the two components of the binary toxins, and the presence of both partners is essential for the activity. Aphid specificity of the binary toxin was further verified by ligand blotting experiment, which identified an ~50 kDa receptor in the brush border membrane vesicles of the cotton aphids only, but not in the lepidopteran insects. Our finding holds a promise of its use in future as a candidate gene for developing transgenic crop plants tolerant against sap-sucking insect pests.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Sattar S,Maiti MKdoi
10.4014/jmb.1105.05030subject
Has Abstractpub_date
2011-09-01 00:00:00pages
937-46issue
9eissn
1017-7825issn
1738-8872pii
JMB021-09-07journal_volume
21pub_type
杂志文章abstract::Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1207.07018
更新日期:2012-11-01 00:00:00
abstract::Activated sludge was sequentially adapted to benzene, toluene, and o-xylene (BTX) to study the effects on the change of microbial community. Sludge adapted to BTX separately degraded each by various rates in the following order; toluene>o-xylene>benzene. Degradation rates were increased after exposure to repeated spik...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::A xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at 75 de...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1203.03045
更新日期:2012-10-01 00:00:00
abstract::In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which th...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1105.05037
更新日期:2011-12-01 00:00:00
abstract:UNLABELLED:We have previously shown that paraquat (PQ)-induced oxidative stress causes dramatic damage in various human cell lines. Naringenin (NG) is an active flavanone, which has been reported to have beneficial bioactivities, including antioxidative, anti-inflammatory, and antitumorigenic activities, with a relativ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1402.02001
更新日期:2014-05-01 00:00:00
abstract::Clitocybin A, an isoindolinone from Clitocybe aurantiaca, was investigated to assess its anti-wrinkle properties, through reactive oxygen species (ROS)-scavenging and elastase inhibitory activities, procollagen synthesis, and matrix metalloproteinase-1 (MMP-1) expression, in human primary dermal fibroblast-neonatal (H...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1702.02050
更新日期:2017-05-28 00:00:00
abstract::Synthetic biology builds programmed biological systems for a wide range of purposes such as improving human health, remedying the environment, and boosting the production of valuable chemical substances. In recent years, the rapid development of synthetic biology has enabled synthetic bacterium-based diagnoses and the...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1904.04016
更新日期:2019-06-28 00:00:00
abstract::Sourdough is made by fermentation of dough by lactic acid bacteria (LAB) and yeast to improve bread properties like volume, flavor, and texture. A Korean traditional sourdough was made by fermenting rice flour with rice wine (makgeolli) and used to make sponge-like bread (jeung-pyun). The aim of this study was to inve...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1708.08003
更新日期:2017-10-28 00:00:00
abstract::To investigate clonal variations of recombinant Chinese hamster ovary (rCHO) clones in response to culture pH and temperature, serum-free suspension cultures of two antibody-producing CHO clones (clones A and B), which were isolated from the same parental clone by the limiting dilution method, were performed in a bior...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa as judged by fast protei...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0911.11022
更新日期:2010-04-01 00:00:00
abstract::Salmonellosis is a form of gastroenteritis caused by Salmonella infection. The main transmission route of salmonellosis has been identified as poorly cooked meat and poultry products contaminated with Salmonella. However, in recent years, the number of outbreaks attributed to contaminated raw produce has increased dra...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2007.07036
更新日期:2020-11-28 00:00:00
abstract::Previous studies have demonstrated that repeated restraint stress in rodents produces increases in depression and anxietylike behaviors and alters the expression of corticotrophinreleasing factor (CRF) in the hypothalamus. The current study focused on the impact of Bupleurum falcatum (BF) extract administration on rep...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1110.10077
更新日期:2012-03-01 00:00:00
abstract::The infection of pandemic influenza viruses such as swine flu (H1N1) and avian flu viruses to the host cells is related to the following two factors: First, the surface protein such as HA (hemagglutinin) and NA (neuraminidase) of the influenza virus. Second, the specific structure of the oligosaccharide [sialic acid(a...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1009.09013
更新日期:2011-05-01 00:00:00
abstract::A Gram-staining-negative, aerobic, light brown pigment bacterium, designated strain CE80T was isolated from marine sponge Callyspongia elegans in Jeju Island, Republic of Korea. Strain CE80T grew optimally at 25°C, in the range of pH 5.0-11.0 (optimum 7.0-8.0), and with 1.0-5.0% NaCl (optimum 1-3% (w/v)). Phylogenetic...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1812.12004
更新日期:2019-12-28 00:00:00
abstract::Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which mi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2002.02059
更新日期:2020-10-28 00:00:00
abstract::A Haematococcus pluvialis strain isolated from the ruins of Ephesus in Turkey was investigated as regards its adaptation to laboratory conditions and maximum growth rate. In the first stage of the experiment, the growth of H. pluvialis was compared in common culture media. Furthermore, in an effort to minimize the cul...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::A bacterium, Pseudomonas aeruginosa BM114, capable of accumulating a blend of medium-chain-length (MCL)- and short-chain-length (SCL)-polyhydroxyalkanoic acid (PHA), was isolated. Salicylic acid (SA), without being metabolized, was found to specifically inhibit only the accumulation of MCL-PHA without affecting cell g...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00
abstract::The adhesion of Campylobacter jejuni to chicken skin, along with the associated morphological changes under aerobic conditions at 4, 25, and 37 degrees C and microaerobic (O2 5%, CO2 10%, N2 85%) conditions, were investigated using confocal laser scanning microscopy (CLSM), flow cytometry, and plate counting. The morp...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-02-01 00:00:00
abstract::Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children wi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1711.11065
更新日期:2018-04-28 00:00:00
abstract::Biologically produced 2,3-butanediol (2,3-BDO) has diverse industrial applications. In this study, schematic isolation and screening procedures were designed to obtain generally regarded as safe (GRAS) and efficient 2,3-BDO producers. Over 4,000 candidate strains were isolated by pretreatment and enrichment, and the i...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1710.10038
更新日期:2018-03-28 00:00:00
abstract::This study was undertaken to evaluate the immunomodulatory effect of dead nano-sized Lactobacillus plantarum (nLp) in RAW 264.7 cells and murine primary splenocytes. nLp is a dead, shrunken, processed form of L. plantarum nF1 isolated from kimchi (a traditional Korean fermented cabbage) and is less than 1 μm in size. ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1511.11001
更新日期:2016-03-01 00:00:00
abstract::Acetic acid is indirectly involved in cell center metabolism, and acetic acid metabolism is the core of central metabolism, affecting and regulating the production of bacteriocin. Bacteriocin is a natural food preservative that has been used in the meat and dairy industries and winemaking. In this paper, the effects o...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1905.05060
更新日期:2019-09-28 00:00:00
abstract::Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 was 8.0, 30 degre...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1004.04034
更新日期:2010-10-01 00:00:00
abstract::The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1301.01004
更新日期:2013-08-01 00:00:00
abstract::The task of improving a fungal strain is highly time-consuming due to the requirement of a large number of flasks in order to obtain a library with enough diversity. In addition, fermentations (particularly those for fungal cells) are typically performed in high-volume (100-250 ml) shake-flasks. In this study, for lar...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1610.10037
更新日期:2017-01-28 00:00:00
abstract::Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of β-glucosidase. In this st...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1305.05011
更新日期:2013-11-28 00:00:00
abstract::Clostridium difficile causes mucosal damage and diarrhea by releasing two exotoxins: toxin A and toxin B. C. difficile colitis is associated with alterations in bowel flora and the failure to mount an effective antibody response. The aim of the current study was to investigate whether antitoxin sera prevent toxin-A-in...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1401.01059
更新日期:2014-05-01 00:00:00
abstract::We isolated and functionally characterized the α- and β- subunits (ApCpnA and ApCpnB) of a chaperonin from Aeropyrum pernix K1. The constructed vectors pET3d- ApCpnA and pET21a-ApCpnB were transformed into E. coli Rosetta (DE3), BL21 (DE3), or CodonPlus (DE3) cells. The expression of ApCpnA (60.7 kDa) and ApCpnB (61.2...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1212.12041
更新日期:2013-06-28 00:00:00
abstract::Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a sing...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1810.10007
更新日期:2018-12-28 00:00:00
abstract::A 2.5 kb aga gene encoding alpha-galactosidase (alpha- Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in ce...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00