Functional characterization of the α- and β-subunits of a group II chaperonin from Aeropyrum pernix K1.

abstract：:Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...

journal_title：Journal of microbiology and biotechnology

authors： Wint NY,Han KK,Yamprayoonswat W,Ruangsuj P,Mangmool S,Promptmas C,Yasawong M

更新日期：2019-01-28 00:00:00

abstract：:To shed light on the genetic differences among food-originated coagulase-negative Staphylococcus (CNS), we performed pan-genome analysis of five species: Staphylococcus carnosus (two strains), Staphylococcus equorum (two strains), Staphylococcus succinus (three strains), Staphylococcus xylosus (two strains), and Staph...

journal_title：Journal of microbiology and biotechnology

authors： Heo S,Lee JS,Lee JH,Jeong DW

更新日期：2020-03-28 00:00:00

abstract：:Total 121 lactic acid bacteria were isolated from homemade Inner Mongolia extra hard Hurood cheese. Seven of these strains, identified as Lactobacillus plantarum, were studied for probiotic characteristics. All seven strains survived at pH 3.0 for 3 h, or in the presence of oxgall at 0.3% or 0.6% for 4 h, but their vi...

journal_title：Journal of microbiology and biotechnology

authors： Zhang J,Zhang X,Zhang L,Zhao Y,Niu C,Yang Z,Li S

更新日期：2014-02-28 00:00:00

abstract：:The gene encoding α-L-arabinofuranosidase (AFase) from Paenibacillus sp. DG-22 was cloned, sequenced, and expressed in Escherichia coli. The AFase gene (abfA) comprises a 1,509 bp open reading frame encoding 502 amino acids with a molecular mass of 56,520 daltons. The deduced amino acid sequence of the gene shows that...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Lee YE

更新日期：2014-02-28 00:00:00

abstract：:Ethyl (S)-3-hydroxy-3-(2-thienyl)propanoate((S)-HEES)acts as a key chiral intermediate for the blockbuster antidepressant drug duloxetine, which canbe achieved viathe stereoselective bioreduction ofethyl 3-oxo-3-(2-thienyl) propanoate (KEES) that containsa 3-oxoacyl structure.The sequences of the short-chain dehydroge...

journal_title：Journal of microbiology and biotechnology

authors： Ren ZQ,Liu Y,Pei XQ,Wu ZL

更新日期：2019-11-28 00:00:00

abstract：:The time-consuming and high-cost preparation of soluble peptide-major histocompatibility complexes (pMHC) currently limits their wide uses in monitoring antigen-specific T cells. The single-chain trimer (SCT) of peptide-β2m-MHC class I heavy chain was developed as an alternative strategy, but its gene fusion is hinder...

journal_title：Journal of microbiology and biotechnology

authors： Xu T,Li X,Wu Y,Shahzad KA,Wang W,Zhang L,Shen C

更新日期：2016-12-28 00:00:00

abstract：:A novel putative toxin-antitoxin segregational stability system named KyAB system was identified in a novel native plasmid pBMB8240 from Bacillus thuringiensis strain YBT-1520, based on sequences homology with other toxin-antitoxin systems, the lethal activity of the KyB putative toxin in Escherichia coli and the stab...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Zhu S,Ye W,Ruan L,Yu Z,Zhao C,Sun M

更新日期：2008-10-01 00:00:00

abstract：:The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for th...

journal_title：Journal of microbiology and biotechnology

authors： Kim JY,Kim HK,Jang HJ,Kim EK,Kim MK

更新日期：2015-04-01 00:00:00

abstract：:Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,124 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative re...

journal_title：Journal of microbiology and biotechnology

authors： Kim SY,Oh CG,Lee YJ,Choi KH,Shin DS,Lee SK,Park KJ,Shin H,Park MS,Lee JH

更新日期：2013-04-01 00:00:00

abstract：:Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. ga...

journal_title：Journal of microbiology and biotechnology

authors： Xu X,Feng L,Han Z,Luo S,Wu A,Xie J

更新日期：2016-09-28 00:00:00

abstract：:Trichoderma reesei is the major filamentous fungus used to produce cellulase and there is huge interest in promoting its ability to produce higher titers of cellulase. Among the many factors affecting cellulase production in T. reesei, the mycelial phenotype is important but seldom studied. Herein, a close homolog of ...

journal_title：Journal of microbiology and biotechnology

authors： Gao F,Li M,Liu W,Bai Y,Tu T,Wang Y,Zhang J,Luo H,Yao B,Huang H,Su X

更新日期：2020-02-28 00:00:00

abstract：:We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...

journal_title：Journal of microbiology and biotechnology

authors： Lim HD,Cheong DE,Shin HJ,Kim GJ

更新日期：2010-11-01 00:00:00

abstract：:Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using nextgeneration sequencing...

journal_title：Journal of microbiology and biotechnology

authors： Huang J,Lin M,Liang S,Qin Q,Liao S,Lu B,Wang Q

更新日期：2020-10-28 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange c...

journal_title：Journal of microbiology and biotechnology

authors： Heo S,Lee SK,Lee CH,Min SG,Park JS,Kim HY

更新日期：2007-04-01 00:00:00

abstract：:Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were...

journal_title：Journal of microbiology and biotechnology

authors： Jin SD,Kim YM,Kang HK,Jung SJ,Kim D

更新日期：2009-11-01 00:00:00

abstract：:Nanoparticles have wide-scale applications in various areas, including medicine, chemistry, electronics, and energy generation. Several physical, biological, and chemical methods have been used for synthesis of silver nanoparticles. Green synthesis of silver nanoparticles using plants provide advantages over other met...

journal_title：Journal of microbiology and biotechnology

authors： Fatima M,Zaidi NU,Amraiz D,Afzal F

更新日期：2016-01-01 00:00:00

abstract：:Gene delivery that provides targeted delivery of therapeutic genes to the cells of a lesion enhances therapeutic efficacy and reduces toxic side effects. This process is especially important in cancer therapy when it is advantageous to avoid unwanted damage to healthy, normal cells. Incorporating cancer-specific ligan...

journal_title：Journal of microbiology and biotechnology

authors： Tu Y,Kim JS

更新日期：2010-04-01 00:00:00

abstract：:Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ΔluxS (ECM101) and ΔluxSΔrelAΔspoT (ECM201...

journal_title：Journal of microbiology and biotechnology

authors： Oh KH,Cho SH

更新日期：2014-03-28 00:00:00

abstract：:This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermen...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Xu Y,Chen X,Zhang LY

更新日期：2015-01-01 00:00:00

abstract：:The bioassay-guided fractionation of MeOH extract from Naematoloma fasciculare afforded a petroleum ether fraction (NFPF) and four known compounds, which showed good antitumor activities to inhibit MCF-7 cell line proliferation in vitro and tumor growth in H(22) implanted mice in vivo. In addition, a number of unsatur...

journal_title：Journal of microbiology and biotechnology

authors： Yan D,Bao HY,Bau T,Li Y,Kim YH

更新日期：2009-10-01 00:00:00

abstract：:Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline ...

journal_title：Journal of microbiology and biotechnology

authors： Ha JS,Lee YM,Choi SL,Song JJ,Shin CS,Kim JH,Lee SG

更新日期：2008-03-01 00:00:00

abstract：:An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwh...

journal_title：Journal of microbiology and biotechnology

authors： Joo HS,Choi JW

更新日期：2012-01-01 00:00:00

abstract：:In the continued search for inhibitors of enoyl-acyl carrier protein (ACP) reductase, we found that four acylbenzenediol sulfate metabolites from Streptomyces sp. AN1761 potently inhibited bacterial enoyl-ACP reductases of Staphylococcus aureus, Streptococcus pneumoniae, and Mycobacterium tuberculosis. Their structure...

journal_title：Journal of microbiology and biotechnology

authors： Kwon YJ,Sohn MJ,Oh T,Cho SN,Kim CJ,Kim WG

更新日期：2013-02-01 00:00:00

abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging phlebovirus of the Phenuiviridae family that has been circulating in East Asian countries, such as China, Japan, and South Korea. Despite the increasing infection rates and relatively high mortality rate, there is limited information available re...

journal_title：Journal of microbiology and biotechnology

authors： Lee JK,Shin OS

更新日期：2020-12-31 00:00:00

abstract：:The bioactivities of boxthron fruits, a source of oriental medicine, are well known, whereas phytochemical studies of the boxthorn stem are rare. In this study, the stem extract of boxthorn (Lycium chinense Miller) and its subfractions were evaluated for their effects on nitric oxide (NO) inhibition and procollagen ty...

journal_title：Journal of microbiology and biotechnology

authors： Gil CS,Chang MS,Eom SH

更新日期：2017-08-28 00:00:00

abstract：:We previously reported that the CopA3 peptide (LLCIALRKK, D-form) originally isolated from the Korean dung beetle has antimicrobial and immunosuppressive effects. However, the high cost of producing the synthetic peptide, especially the D-form, has limited the development of CopA3 for therapeutic purposes. Here, we in...

journal_title：Journal of microbiology and biotechnology

authors： Yoon IN,Hong J,Zhang P,Hwang JS,Kim H

更新日期：2017-02-28 00:00:00

abstract：:It has been reported that Tat-SOD can be directly transduced into mammalian cells and skin and acts as a potential therapeutic protein in various diseases. To isolate the compound that can enhance the transduction efficiency of Tat-SOD, we screened a number of natural products. 3-O-[beta-D-Glucopyranosyl(1-->4)-alpha-...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Kim SY,Kim DW,Jang SH,Lim SS,Kwon HJ,Kang TC,Won MH,Kang IJ,Lee KS,Park J,Eum WS,Choi SY

更新日期：2008-09-01 00:00:00

abstract：:The present study shows that DR1114 (Hsp20), a small heat shock protein of the radiationresistant bacterium Deinococcus radiodurans, enhances tolerance to hydrogen peroxide (H2O2) stress when expressed in Escherichia coli. A protein profile comparison showed that E. coli cells overexpressing D. radiodurans Hsp20 (EC-p...

journal_title：Journal of microbiology and biotechnology

authors： Singh H,Appukuttan D,Lim S

更新日期：2014-08-01 00:00:00

abstract：:A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 ...

journal_title：Journal of microbiology and biotechnology

authors： Wen C,Guo W,Chen X

更新日期：2014-10-01 00:00:00