Purification and identification of a novel antifungal protein secreted by Penicillium citrinum from the Southwest Indian Ocean.

Abstract:

:A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 culture supernatants with ammonium sulfate and ion-exchange chromatography, an antifungal protein (PcPAF) was purified. The N-terminal amino acid sequence analysis showed that PcPAF might be an unknown antifungal protein. PcPAF displayed antifungal activity against Trichoderma viride, Fusarium oxysporum, Paecilomyces variotii, and Alternaria longipes at minimum inhibitory concentrations of 1.52, 6.08, 3.04, and 6.08 µg/disc, respectively. PcPAF possessed high thermostability and had a certain extent of protease and metal ion resistance. The results suggested that PcPAF may represent a novel antifungal protein with potential application in controlling plant pathogenic fungal infection.

journal_name

J Microbiol Biotechnol

authors

Wen C,Guo W,Chen X

doi

10.4014/jmb.1405.05008

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

1337-45

issue

10

eissn

1017-7825

issn

1738-8872

pii

10.4014/jmb.1405.05008

journal_volume

24

pub_type

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