Abstract：
:Different endophytic fungi isolated from Himalayan Yew plants were tested for their ability to produce taxol. The BAPT gene (C-13 phenylpropanoid side chain-CoA acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxol-producing endophytic fungi. Taxol extracted from fungal strain TBPJ-B was identified by HPLC and MS analysis. Strain TBPJ-B was identified as Fusarium redolens based on the morphology and internal transcribed spacer region of nrDNA analysis. HPLC quantification of fungal taxol showed that F. redolens was capable of producing 66 μg/l of taxol in fermentation broth. The antitumour activity of the fungal taxol was tested by potato disc tumor induction assay using Agrobacterium tumefaciens as the tumor induction agent. The present study results showed that PCR amplification of genes involved in taxol biosynthesis is an efficient and reliable method for prescreening taxol-producing fungi. We are reporting for the first time the production of taxol by F. redolens from Taxus baccata L. subsp. wallichiana (Zucc.) Pilger. This study offers important information and a new source for the production of the important anticancer drug taxol by endophytic fungus fermentation.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Garyali S,Kumar A,Reddy MSdoi
10.4014/jmb.1305.05070subject
Has Abstractpub_date
2013-10-28 00:00:00pages
1372-80issue
10eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1305.05070journal_volume
23pub_type
杂志文章abstract：:Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cell...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1107.07022
更新日期：2011-12-01 00:00:00
abstract：:In the past, reactive oxygen species (ROS) have been considered a harmful byproduct of aerobic metabolism. However, accumulating evidence implicates redox homeostasis, which maintains appropriate ROS levels, in cell proliferation and differentiation in plants and animals. Similarly, ROS generation and signaling are in...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.4014/jmb.1407.07072
更新日期：2014-11-28 00:00:00
abstract：:In the course of screening for microbes with nematicidal activity, we found that Enterobacter asburiae HK169 displayed promising nematicidal activity against the root-knot nematode Meloidogyne incognita, along with plant growth-promoting properties. Soil drenching of a culture of HK169 reduced gall formation by 66% wh...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1801.01021
更新日期：2018-06-28 00:00:00
abstract：:The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the bind...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1506.06079
更新日期：2015-11-01 00:00:00
abstract：:The effects of algal inoculation on chemical oxygen demand (COD) and total nitrogen (TN) removal, and indigenous bacterial dynamics were investigated in municipal wastewater. Experiments were conducted with municipal wastewater inoculated with either Chlorella vulgaris AG10032, Selenastrum gracile UTEX 325, or Scenede...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1512.12067
更新日期：2016-05-28 00:00:00
abstract：:A response surface model was developed for predicting the growth rates of Staphylococcus aureus in tryptic soy broth (TSB) medium as a function of combined effects of temperature, pH, and NaCl. The TSB containing six different concentrations of NaCl (0, 2, 4, 6, 8, and 10%) was adjusted to an initial of six different ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:In this study, we investigated the feasibility of producing bioethanol from the hydrolysate of rape stem. Specifically, the most ideal yeast strain was screened, and the microaeration was performed by surface aeration on a liquid medium surface. Among the yeast strains examined, Pichia stipitis CBS 7126 displayed the ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1008.08001
更新日期：2011-01-01 00:00:00
abstract：:An extensive investigation was carried out to describe the kinetics of cell growth, substrate consumption, and product formation in the batch fermentation using starch as substrate. Evaluation of intrinsic kinetic parameters was carried out using a best-fit unstructured model. A nonlinear regression technique was appl...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1206.06015
更新日期：2012-12-01 00:00:00
abstract：:To investigate clonal variations of recombinant Chinese hamster ovary (rCHO) clones in response to culture pH and temperature, serum-free suspension cultures of two antibody-producing CHO clones (clones A and B), which were isolated from the same parental clone by the limiting dilution method, were performed in a bior...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：:To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：:The antagonistic effect of Trichoderma harzianum on a range of seed-borne fungal pathogens of wheat (viz. Fusarium graminearum, Bipolaris sorokiniana, Aspergillus spp., and Penicillium spp.) was assessed. The potential of T. harzianum as a biocontrol agent was tested in vitro and under field conditions. Coculture of t...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1107.07063
更新日期：2012-05-01 00:00:00
abstract：:Bacteriophages have gained substantial attention as biocontrol and biorecognition agents, substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is hi...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1711.11017
更新日期：2017-12-28 00:00:00
abstract：:The gut microbiome influences the health and well-being of dogs. However, little is known about the impact of breed on the fecal microbiome composition in dogs. Therefore, we aimed to investigate the differences in the fecal microbiome in three breeds of dog fed and housed under the same conditions, namely eight Malte...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1906.06048
更新日期：2019-12-28 00:00:00
abstract：:Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a sing...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1810.10007
更新日期：2018-12-28 00:00:00
abstract：:Polyester cloth (PC) was selected as a prospective inexpensive substitute separator material for microbial fuel cells (MFCs). PC was compared with a traditional Nafion proton exchange membrane (PEM) as an MFC separator by analyzing its physical and electrochemical properties. A single layer of PC showed higher mass tr...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1608.08040
更新日期：2016-12-28 00:00:00
abstract：:Optimal conditions for a high cell density fermentation were investigated in a recombinant Escherichia coli producing salmosin, a platelet aggregation inhibitor. The optimized carbon and nitrogen sources were glycerol 10 g/l, yeast extract 30 g/l, and bacto-tryptone 10 g/l, yielding the dry cell weight (DCW) of 10.61 ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1105.05014
更新日期：2011-10-01 00:00:00
abstract：:The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for th...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1409.09079
更新日期：2015-04-01 00:00:00
abstract：:The present work aims to use a biofilter technology (aerated submerged filters) for the aerobic transformation at laboratory-scale of olive washing water (OWW) generated in the first steps of olive oil processing, as well as the genetic profiling and identification to the species level of the bacteria involved in the ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：:Chlamydia possesses a conserved 7.5 kb plasmid that is known to play an important role in chlamydial pathogenesis, since some chlamydial organisms lacking the plasmid are attenuated. The chlamydial transformation system developed recently required the use of plasmid-free organisms. Thus, the generation and identificat...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1503.03072
更新日期：2015-10-01 00:00:00
abstract：:Isomaltooligosaccharide (IMO) is a promising dietary component with prebiotic effect, and the long-chain IMOs are preferred to short chain ones owing to the longer persistence in the colon. To establish the optimal process for synthesis of long-chain IMOs, we systematically examined the reaction condition of dextransu...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-06-01 00:00:00
abstract：:The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and fi...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
abstract：:The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1301.01004
更新日期：2013-08-01 00:00:00
abstract：:Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomyco...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1004.04007
更新日期：2010-11-01 00:00:00
abstract：:We recently reported that the antimicrobial peptide Lumbricusin (NH2-RNRRWCIDQQA), isolated from the earthworm, increases cell proliferation in neuroblastoma SH-SY5Y cells. Here, we investigated whether Lumbricusin has neurotropic activity in mouse neural stem cells (MNSCs) and a protective effect in a mouse model of ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1507.07011
更新日期：2015-10-01 00:00:00
abstract：:Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by a...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.0804.254
更新日期：2009-04-01 00:00:00
abstract：:We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1007.07015
更新日期：2010-11-01 00:00:00
abstract：:An efficient and simple fermentation process was developed for the production of gamma-amminobutyric acid (GABA) by Lactobacillus sakei B2-16. When the L. sakei B2-16 was cultivated in the rice bran extracts medium containing 4% sucrose, 1% yeast extract and 12% monosodium glutamate, the maximum GABA concentration rea...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:A gene (acas) designated as alpha-amylase was cloned from Arthrobacter chlorophenolicus A6. The multiple amino acid sequence analysis and functional expression of acas revealed that this gene really encoded an amylosucrase (ASase) instead of alpha-amylase. In fact, the recombinant enzyme exhibited typical ASase activi...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1201.01056
更新日期：2012-09-01 00:00:00
abstract：:The first gene (alpha-gal1) encoding an extracellular alpha-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The alpha-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:The synergy effect of trisodium phosphate (TSP) and ethanol against murine norovirus 1 (MNV-1), as a surrogate for human noroviruses, on fresh produces was evaluated. More than 2% (w/v) of TSP effectively inactivated MNV-1. The single treatment of 1% TSP or 30% ethanol for 30 min was not effective on MNV-1; however, c...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1503.03032
更新日期：2015-12-28 00:00:00
