Kinetic studies of alkaline protease from Bacillus licheniformis NCIM-2042.

Abstract:

:An extensive investigation was carried out to describe the kinetics of cell growth, substrate consumption, and product formation in the batch fermentation using starch as substrate. Evaluation of intrinsic kinetic parameters was carried out using a best-fit unstructured model. A nonlinear regression technique was applied for computational purpose. The Andrew's model showed a comparatively better R2 value among all tested models. The values of specific growth rate (micronmax), saturation constant (KS), inhibition constant (KI), and YX/S were found to be 0.109 h-1, 11.1 g/l, 0.012 g/l, and 1.003, respectively. The Leudeking- Piret model was used to study the product formation kinetics and the process was found to be growth-associated. The growth-associated constant (alpha) for protease production was sensitive to substrate concentration. Its value was fairly constant up to a substrate concentration of 30.8 g/l, and then decreased.

journal_name

J Microbiol Biotechnol

authors

Bhunia B,Basak B,Bhattacharya P,Dey A

doi

10.4014/jmb.1206.06015

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1758-66

issue

12

eissn

1017-7825

issn

1738-8872

pii

JMB022-12-21

journal_volume

22

pub_type

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