Abstract：
:The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for the unknown antigen. To avoid the loss of target yeast clones that express proteins at a low frequency in the cDNA library, a comprehensive monitoring system based on magnetic-activated cell sorting, fluorescence-activated cell sorting, and immunofluorescence was established, and a small number of target yeast cells was successfully enriched. These results showed that our optimized method has potential application for identifying rare unknown antigens of the human monoclonal antibody.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Kim JY,Kim HK,Jang HJ,Kim EK,Kim MKdoi
10.4014/jmb.1409.09079subject
Has Abstractpub_date
2015-04-01 00:00:00pages
547-53issue
4eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1409.09079journal_volume
25pub_type
杂志文章abstract：:Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fe...
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abstract：:Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this...
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更新日期：2017-06-28 00:00:00
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journal_title：Journal of microbiology and biotechnology
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更新日期：2011-06-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1608.08019
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更新日期：2007-12-01 00:00:00
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更新日期：2008-05-01 00:00:00
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更新日期：2009-10-01 00:00:00
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更新日期：2007-01-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2015-02-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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更新日期：2008-03-01 00:00:00
abstract：:The application of steroids has steadily increased thanks to their therapeutic effects. However, alternatives are required due their severe side effects; thus, studies on the activities of steroid derivatives are underway. Sugar derivatives of nandrolone, which is used to treat breast cancer, as well as cortisone and ...
journal_title：Journal of microbiology and biotechnology
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