Characterization of Bacillus polyfermenticus KJS-2 as a probiotic.

Abstract:

:The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be 60.71+/-4.9%. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of 140+/-7.9% and 108+/-3.5%, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately 65+/-0.6%.

journal_name

J Microbiol Biotechnol

authors

Kim KM,Kim MJ,Kim DH,Park YS,Kang JS

doi

10.4014/jmb.0903.113

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

1013-8

issue

9

eissn

1017-7825

issn

1738-8872

pii

JMB019-09-22

journal_volume

19

pub_type

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