Biochemical Characterization of Alkaliphilic Cyclodextran Glucanotransferase from an Alkaliphilic Bacterium, Paenibacillus daejeonensis.

abstract：:In order to induce high levels of protein secretion, we have constructed a recombinant plasmid, designated pBP244, into which was incorporated key components of the type-II Sec-dependent secretion system, including LepB (signal peptidase), SecA (ATPase), and SecB (chaperone). The biological activities of the LepB, Sec...

journal_title：Journal of microbiology and biotechnology

authors： Kim SW,Kim YH,Yoo AY,Yu JE,Hur J,Lee JH,Cha J,Kang HY

更新日期：2007-08-01 00:00:00

abstract：:Dephosphocoenzyme A (CoaE) catalyzes the last step in the biosynthesis of the cofactor coenzyme A. In this study, we report the identification and application of CoaE from Stretomyces peucetius ATCC27952. After expression of coaE, the protein was found to have a molecular mass of 28.6 kDa. Purification of the His-tagg...

journal_title：Journal of microbiology and biotechnology

authors： Lee NR,Rimal H,Lee JH,Oh TJ

更新日期：2014-09-01 00:00:00

abstract：:Fungal biomass and fungal extract of the nonpathogenic fungus Neurospora crassa were successfully used as reducing agents for the biosynthesis of platinum nanoparticles (PtNPs). The experiment was carried out by exposing the fungal biomass or the fungal extract to a 0.001 M precursor solution of hexachloroplatinic(IV)...

journal_title：Journal of microbiology and biotechnology

authors： Castro-Longoria E,Moreno-Velázquez SD,Vilchis-Nestor AR,Arenas-Berumen E,Avalos-Borja M

更新日期：2012-07-01 00:00:00

abstract：:The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex...

journal_title：Journal of microbiology and biotechnology

authors： Bideshi DK,Waldrop G,Fernandez-Luna MT,Diaz-Mendoza M,Wirth MC,Johnson JJ,Park HW,Federici BA

更新日期：2013-08-01 00:00:00

abstract：:We inoculated different combinations of three starter candidates of Bacillus licheniformis, Staphylococcus succinus, and Tetragenococcus halophilus, into sterilized soybeans to predict their contributions to volatile compounds production through soybean fermentation. Simultaneously, we added NaCl to soybean cultures t...

journal_title：Journal of microbiology and biotechnology

authors： Jeong DW,Lee H,Jeong K,Kim CT,Shim ST,Lee JH

更新日期：2019-02-28 00:00:00

abstract：:The identification of bacterial pathogens to humans is critical for environmental microbial risk assessment. However, current methods for identifying pathogens in environmental samples are limited in their ability to detect highly diverse bacterial communities and accurately differentiate pathogens from commensal bact...

journal_title：Journal of microbiology and biotechnology

authors： Yang J,Howe A,Lee J,Yoo K,Park J

更新日期：2020-09-28 00:00:00

abstract：:In the continued search for inhibitors of enoyl-acyl carrier protein (ACP) reductase, we found that four acylbenzenediol sulfate metabolites from Streptomyces sp. AN1761 potently inhibited bacterial enoyl-ACP reductases of Staphylococcus aureus, Streptococcus pneumoniae, and Mycobacterium tuberculosis. Their structure...

journal_title：Journal of microbiology and biotechnology

authors： Kwon YJ,Sohn MJ,Oh T,Cho SN,Kim CJ,Kim WG

更新日期：2013-02-01 00:00:00

abstract：:Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction proce...

journal_title：Journal of microbiology and biotechnology

authors： Nie Y,Xu Y,Hu QS,Xiao R

更新日期：2009-01-01 00:00:00

abstract：:The infection of pandemic influenza viruses such as swine flu (H1N1) and avian flu viruses to the host cells is related to the following two factors: First, the surface protein such as HA (hemagglutinin) and NA (neuraminidase) of the influenza virus. Second, the specific structure of the oligosaccharide [sialic acid(a...

journal_title：Journal of microbiology and biotechnology

authors： Yoo ES

更新日期：2011-05-01 00:00:00

abstract：:Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanopa...

journal_title：Journal of microbiology and biotechnology

authors： Han AL,Park SH,Park MS

更新日期：2017-02-28 00:00:00

abstract：:In this study, DEAE-corncobs [delignified corncob grits derivatized with 2-(diethylamino)ethyl chloride hydrochloride (DEAE·HCl)] were prepared as a carrier to immobilize yeast (Saccharomyces cerevisiae) for ethanol production. The immobilized yeast cell reactor produced ethanol under optimized DEAE·HCl derivatization...

journal_title：Journal of microbiology and biotechnology

authors： Lee SE,Lee CG,Kang DH,Lee HY,Jung KH

更新日期：2012-12-01 00:00:00

abstract：:Two new glucosides (1 and 2) of geldanamycin (GA) analogs were obtained from in vitro glycosylation by UDP-glycosyltransferase (YjiC). Based on spectroscopic (HR-ESI-MS, 1D, and 2D-NMR) analyses, the glucosides were elucidated as 4,5-dihydro-7-O-descarbamoyl-7- hydroxyl GA-7-O-β-D-glucoside (1) and ACDL3172-18-O-β-D-g...

journal_title：Journal of microbiology and biotechnology

authors： Huo Q,Li HM,Lee JK,Li J,Ma T,Zhang X,Dai Y,Hong YS,Wu CZ

更新日期：2016-01-01 00:00:00

abstract：:The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and fi...

journal_title：Journal of microbiology and biotechnology

authors： Kim HL,Shin E,Kim HM,Go H,Roh J,Bae J,Lee K

更新日期：2007-10-01 00:00:00

abstract：:Drought stress is threatening the growth and productivity of many economical crops. Therefore, it is necessary to establish innovative and efficient approaches for improving crop growth and productivity. Here we investigated the potentials of the cell-free extract of Actinobacteria (Ac) isolated from a semi-arid habit...

journal_title：Journal of microbiology and biotechnology

authors： Warrad M,Hassan YM,Mohamed MSM,Hagagy N,Al-Maghrabi OA,Selim S,Saleh AM,AbdElgawad H

更新日期：2020-08-28 00:00:00

abstract：:Five fusion proteins between Z domains derived from Staphylococcal Protein A and Green Fluorescent Protein or Human Proinsulin were produced on the periplasm of Escherichia coli. The effects of the molecular weight and amino acid composition of the translocated peptide, culture medium composition, and growth phase of ...

journal_title：Journal of microbiology and biotechnology

authors： Mergulhão FJ,Monteiro GA

更新日期：2007-08-01 00:00:00

abstract：:Glucagon, a peptide hormone produced by alpha-cells of Langerhans islets, is a physiological antagonist of insulin and stimulator of its secretion. In order to improve its bioactivity, we modified its structure at the C-terminus by amidation catalyzed by a recombinant amidase in bacterial cells. The human gene coding ...

journal_title：Journal of microbiology and biotechnology

authors： Qi X,Jiang R,Yao C,Zhang R,Li Y

更新日期：2008-06-01 00:00:00

abstract：:This study was undertaken to evaluate the immunomodulatory effect of dead nano-sized Lactobacillus plantarum (nLp) in RAW 264.7 cells and murine primary splenocytes. nLp is a dead, shrunken, processed form of L. plantarum nF1 isolated from kimchi (a traditional Korean fermented cabbage) and is less than 1 μm in size. ...

journal_title：Journal of microbiology and biotechnology

authors： Lee HA,Kim H,Lee KW,Park KY

更新日期：2016-03-01 00:00:00

abstract：:Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 was 8.0, 30 degre...

journal_title：Journal of microbiology and biotechnology

authors： Jin DC,Liang RX,Dai QY,Zhang RY,Wu XL,Chao WL

更新日期：2010-10-01 00:00:00

abstract：:This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Sung JY,Cho HH,Kwon KC,Koo SH

更新日期：2014-06-28 00:00:00

abstract：:Two novel avian beta-defensins (AvBDs) isolated from duck liver were characterized and their homologies with other AvBDs were analyzed. They were shown to be duck AvBD9 and AvBD10. The mRNA expression of the two genes was analyzed in 17 different tissues from 1-28-dayold ducks. AvBD9 was differentially expressed in th...

journal_title：Journal of microbiology and biotechnology

authors： Ma D,Liao W,Wang R,Han Z,Liu S

更新日期：2009-11-01 00:00:00

abstract：:Nargenicin A1 is a 28-membered polyketide macrolide, with antibacterial activity against methicillin-resistant Staphylococcus aureus, produced by Nocardia sp. CS682. In this study, the production of nargenicin A1 was improved by enhancing the supply of different biosynthetic precursors. In Nocardia sp. CS682 (KCTC1129...

journal_title：Journal of microbiology and biotechnology

authors： Koju D,Maharjan S,Dhakal D,Yoo JC,Sohng JK

更新日期：2012-08-01 00:00:00

abstract：:Since 1990, many nucleic acid expression platforms consisting of DNA or RNA have been developed. However, although RNA expression platforms have been relatively neglected, several such platforms capped at the 5' end of RNA by an anti-reverse cap analog have now been developed. At the same time, the capping reaction is...

journal_title：Journal of microbiology and biotechnology

authors： Ko HL,Park HJ,Kim J,Kim H,Youn H,Nam JH

更新日期：2019-01-28 00:00:00

abstract：:The study objective was to prepare biodegradable branched dextran microspheres encapsulated with His-tagged interferon-alpha (BDM-hIFN-alpha) and evaluate its activity in vitro and in vivo. The glycidyl methacrylate derivatized dextrans (Dex-GMA) as precursor was primarily synthesized by substituting hydroxyl groups o...

journal_title：Journal of microbiology and biotechnology

authors： Hong H,Jo JR,Yeon JH,Hong JT,Jung KH,Yoo SK,Jang BC

更新日期：2011-02-01 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive diseas...

journal_title：Journal of microbiology and biotechnology

authors： Jung SJ,Seo ES,Yun SI,Minh BN,Jin SD,Ryu HJ,Kim D

更新日期：2011-07-01 00:00:00

abstract：:In this study, we investigated the potential of using sediment bioelectrochemical systems (SBESs) for in situ treatment of the water and sediment in brackish aquaculture ponds polluted with uneaten feed. An SBES integrated into a laboratory-scale tank simulating a brackish aquaculture pond was established. This test t...

journal_title：Journal of microbiology and biotechnology

authors： Pham HT,Tran HT,Vu LT,Dang HT,Nguyen TTT,Dang THT,Nguyen MTT,Nguyen HQ,Kim BH

更新日期：2019-07-28 00:00:00

abstract：:Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity ...

journal_title：Journal of microbiology and biotechnology

authors： Lin X,Liu S,Xie G,Chen J,Li P,Chen J

更新日期：2016-11-28 00:00:00

abstract：:Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and termina...

journal_title：Journal of microbiology and biotechnology

authors： Park HD,Noguera DR

更新日期：2008-08-01 00:00:00

abstract：:Although most algae cultivation systems are operated in suspended culture, an attached growth system can offer several advantages over suspended systems. Algal cultivation becomes light-limited as the microalgal concentration increases in the suspended system; on the other hand, sunlight penetrates deeper and stronger...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Oh HM,Jo BH,Lee SA,Shin SY,Kim HS,Lee SH,Ahn CY

更新日期：2014-11-28 00:00:00

abstract：:The effects of two different sugars (glucose and xylose) on the expression levels and patterns of xylose reductase (xyl1), xylitol dehydrogenase (xyl2) and xylulokinase (xyl3) genes were analyzed using Pichia stipitis. A significant increase in mRNA levels of xyl1 was observed after 6 hours growth in culture condition...

journal_title：Journal of microbiology and biotechnology

authors： Han JH,Park JY,Kang HW,Choi GW,Chung BW,Min J

更新日期：2010-05-01 00:00:00

abstract：:The total methylotrophic population associated with rice plants from different cultivars was enumerated at three different stages: vegetative, flowering, and harvesting. The bacterial population in the leaf, rhizosphere soil, endophytic in the stem and roots, and epiphytic in the florets and grains were determined fro...

journal_title：Journal of microbiology and biotechnology

authors： Madhaiyan M,Poonguzhali S,Sa T

更新日期：2007-10-01 00:00:00