Purification of capsular polysaccharide produced by Streptococcus pneumoniae serotype 19A.

Abstract:

:Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive disease. Among the more than 90 different S. pneumoniae serotypes, serotype 19A is globally very prevalent. A simplified purification procedure including adjustment of cell lysate pH to 4.5, fractionation with 50- 80% ethanol, and dialysis rendered capsular polysaccharide (CPS) in a yield of 31.32 +/- 3.11 mg from 1 l culture (75% recovery after lyses). The product contained only 69.6 microng of protein (99.78% purity) and 0.8 mg (sum of the precipitants from 50~60%, 60~70%, and 70~80%) of nucleic acid (97.45% purity). The purified CPS was conjugated with bovine serum albumin; the product size ranged from 100 to 180 kDa.

journal_name

J Microbiol Biotechnol

authors

Jung SJ,Seo ES,Yun SI,Minh BN,Jin SD,Ryu HJ,Kim D

doi

10.4014/jmb.1010.10043

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

734-8

issue

7

eissn

1017-7825

issn

1738-8872

pii

JMB021-07-10

journal_volume

21

pub_type

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